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- EMDB-31009: CryoEM structure of the human Kv4.2-KChIP1 complex, intracellular... -

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Basic information

Entry
Database: EMDB / ID: EMD-31009
TitleCryoEM structure of the human Kv4.2-KChIP1 complex, intracellular region
Map datamap A
Sample
  • Cell: CryoEM structure of the human Kv4.2-KChIP1 complex
    • Protein or peptide: Potassium voltage-gated channel subfamily D member 2
    • Protein or peptide: Kv channel-interacting protein 1
Function / homology
Function and homology information


Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / membrane repolarization / Voltage gated Potassium channels / postsynaptic specialization membrane / regulation of potassium ion transmembrane transport / anchoring junction / regulation of heart contraction ...Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / membrane repolarization / Voltage gated Potassium channels / postsynaptic specialization membrane / regulation of potassium ion transmembrane transport / anchoring junction / regulation of heart contraction / action potential / neuronal cell body membrane / locomotor rhythm / voltage-gated potassium channel activity / plasma membrane raft / potassium channel activity / neuronal action potential / GABA-ergic synapse / potassium channel regulator activity / voltage-gated potassium channel complex / sensory perception of pain / potassium ion transmembrane transport / muscle contraction / protein homooligomerization / cytoplasmic side of plasma membrane / cellular response to hypoxia / perikaryon / chemical synaptic transmission / postsynaptic membrane / transmembrane transporter binding / dendritic spine / dendrite / neuronal cell body / glutamatergic synapse / calcium ion binding / metal ion binding / plasma membrane / cytoplasm
Similarity search - Function
Potassium channel, voltage dependent, Kv4.2 / Potassium channel, voltage dependent, Kv4 / Shal-type voltage-gated potassium channels, N-terminal / Potassium channel, voltage dependent, Kv4, C-terminal / Shal-type voltage-gated potassium channels, N-terminal / Domain of unknown function (DUF3399) / Recoverin family / Potassium channel, voltage dependent, Kv / Potassium channel tetramerisation-type BTB domain / BTB/POZ domain ...Potassium channel, voltage dependent, Kv4.2 / Potassium channel, voltage dependent, Kv4 / Shal-type voltage-gated potassium channels, N-terminal / Potassium channel, voltage dependent, Kv4, C-terminal / Shal-type voltage-gated potassium channels, N-terminal / Domain of unknown function (DUF3399) / Recoverin family / Potassium channel, voltage dependent, Kv / Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / EF-hand domain pair / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / Voltage-dependent channel domain superfamily / SKP1/BTB/POZ domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / Ion transport domain / Ion transport protein / EF-hand domain / EF-hand domain pair
Similarity search - Domain/homology
Kv channel-interacting protein 1 / Potassium voltage-gated channel subfamily D member 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsKise Y / Nureki O
Funding support Japan, 1 items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS) Japan
CitationJournal: Nature / Year: 2021
Title: Structural basis of gating modulation of Kv4 channel complexes.
Authors: Yoshiaki Kise / Go Kasuya / Hiroyuki H Okamoto / Daichi Yamanouchi / Kan Kobayashi / Tsukasa Kusakizako / Tomohiro Nishizawa / Koichi Nakajo / Osamu Nureki /
Abstract: Modulation of voltage-gated potassium (Kv) channels by auxiliary subunits is central to the physiological function of channels in the brain and heart. Native Kv4 tetrameric channels form ...Modulation of voltage-gated potassium (Kv) channels by auxiliary subunits is central to the physiological function of channels in the brain and heart. Native Kv4 tetrameric channels form macromolecular ternary complexes with two auxiliary β-subunits-intracellular Kv channel-interacting proteins (KChIPs) and transmembrane dipeptidyl peptidase-related proteins (DPPs)-to evoke rapidly activating and inactivating A-type currents, which prevent the backpropagation of action potentials. However, the modulatory mechanisms of Kv4 channel complexes remain largely unknown. Here we report cryo-electron microscopy structures of the Kv4.2-DPP6S-KChIP1 dodecamer complex, the Kv4.2-KChIP1 and Kv4.2-DPP6S octamer complexes, and Kv4.2 alone. The structure of the Kv4.2-KChIP1 complex reveals that the intracellular N terminus of Kv4.2 interacts with its C terminus that extends from the S6 gating helix of the neighbouring Kv4.2 subunit. KChIP1 captures both the N and the C terminus of Kv4.2. In consequence, KChIP1 would prevent N-type inactivation and stabilize the S6 conformation to modulate gating of the S6 helices within the tetramer. By contrast, unlike the reported auxiliary subunits of voltage-gated channel complexes, DPP6S interacts with the S1 and S2 helices of the Kv4.2 voltage-sensing domain, which suggests that DPP6S stabilizes the conformation of the S1-S2 helices. DPP6S may therefore accelerate the voltage-dependent movement of the S4 helices. KChIP1 and DPP6S do not directly interact with each other in the Kv4.2-KChIP1-DPP6S ternary complex. Thus, our data suggest that two distinct modes of modulation contribute in an additive manner to evoke A-type currents from the native Kv4 macromolecular complex.
History
DepositionFeb 28, 2021-
Header (metadata) releaseOct 13, 2021-
Map releaseOct 13, 2021-
UpdateFeb 16, 2022-
Current statusFeb 16, 2022Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0336
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  • Surface view colored by cylindrical radius
  • Surface level: 0.0336
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  • Surface view with fitted model
  • Atomic models: PDB-7e83
  • Surface level: 0.0336
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7e83
  • Imaged by Jmol
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_31009.png

Downloads & links

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Map

FileDownload / File: emd_31009.map.gz / Format: CCP4 / Size: 36.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmap A
Voxel sizeX=Y=Z: 1.00226 Å
Density
Contour LevelBy AUTHOR: 0.0336 / Movie #1: 0.0336
Minimum - Maximum-0.17440513 - 0.2720796
Average (Standard dev.)0.0008286828 (±0.007749031)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions212212212
Spacing212212212
CellA=B=C: 212.47911 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.00225943396231.00225943396231.0022594339623
M x/y/z212212212
origin x/y/z0.0000.0000.000
length x/y/z212.479212.479212.479
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS212212212
D min/max/mean-0.1740.2720.001

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Supplemental data

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Mask #1

Fileemd_31009_msk_1.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: #1

Fileemd_31009_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_31009_half_map_2.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Sample components

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Entire : CryoEM structure of the human Kv4.2-KChIP1 complex

EntireName: CryoEM structure of the human Kv4.2-KChIP1 complex
Components
  • Cell: CryoEM structure of the human Kv4.2-KChIP1 complex
    • Protein or peptide: Potassium voltage-gated channel subfamily D member 2
    • Protein or peptide: Kv channel-interacting protein 1

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Supramolecule #1: CryoEM structure of the human Kv4.2-KChIP1 complex

SupramoleculeName: CryoEM structure of the human Kv4.2-KChIP1 complex / type: cell / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Potassium voltage-gated channel subfamily D member 2

MacromoleculeName: Potassium voltage-gated channel subfamily D member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.755738 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AAGVAAWLPF ARAAAIGWMP VASGPMPAPP RQERKRTQDA LIVLNVSGTR FQTWQDTLER YPDTLLGSSE RDFFYHPETQ QYFFDRDPD IFRHILNFYR TGKLHYPRHE CISAYDEELA FFGLIPEIIG DCCYEEYKDR RRENAERLQD DADTDTAGES A LPTMTARQ ...String:
AAGVAAWLPF ARAAAIGWMP VASGPMPAPP RQERKRTQDA LIVLNVSGTR FQTWQDTLER YPDTLLGSSE RDFFYHPETQ QYFFDRDPD IFRHILNFYR TGKLHYPRHE CISAYDEELA FFGLIPEIIG DCCYEEYKDR RRENAERLQD DADTDTAGES A LPTMTARQ RVWRAFENPH TSTMALVFYY VTGFFIAVSV IANVVETVPC GSSPGHIKEL PCGERYAVAF FCLDTACVMI FT VEYLLRL AAAPSRYRFV RSVMSIIDVV AILPYYIGLV MTDNEDVSGA FVTLRVFRVF RIFKFSRHSQ GLRILGYTLK SCA SELGFL LFSLTMAIII FATVMFYAEK GSSASKFTSI PAAFWYTIVT MTTLGYGDMV PKTIAGKIFG SICSLSGVLV IALP VPVIV SNFSRIYHQN QRADKRRAQK KARLARIRAA KSGSANAYMQ SKRSGLLSNQ LQSSEDEQAF VSKSGSSFET QHHHL LHCL EKTTNHEFVD

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Macromolecule #2: Kv channel-interacting protein 1

MacromoleculeName: Kv channel-interacting protein 1 / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.242941 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
PEGLEQLEAQ TNFTKRELQV LYRGFKNECP SGVVNEDTFK QIYAQFFPHG DASTYAHYLF NAFDTTQTGS VKFEDFVTAL SILLRGTVH EKLRWTFNLY DINKDGYINK EEMMDIVKAI YDMMGKYTYP VLKEDTPRQH VDVFFQKMDK NKDGIVTLDE F LESCQEDD NIMRSLQLFQ NVM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 48.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 434296
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-7e83:
CryoEM structure of the human Kv4.2-KChIP1 complex, intracellular region

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