National Natural Science Foundation of China (NSFC)
31600593
China
National Natural Science Foundation of China (NSFC)
31870132, 82072237
China
Citation
Journal: iScience / Year: 2021 Title: Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. Authors: Zhenlin Ouyang / Fang Zheng / Li Zhu / Jan Felix / Di Wu / Ke Wu / Irina Gutsche / Yi Wu / Peter M Hwang / Junjun She / Yurong Wen / Abstract: Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The two-component regulatory system AdeRS is made ...Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The two-component regulatory system AdeRS is made up of a sensor histidine kinase AdeS and a cognate response regulator AdeR, which together reduce repression of the multidrug-resistant efflux pump AdeABC. Herein we demonstrate that an N-terminal intrinsically disordered tail in AdeR is important for the upregulation of expression, although it greatly increases the susceptibility of AdeR to proteasome-mediated degradation. We also show that AdeS assembles into a hexameric state that is necessary for its full histidine kinase activity, which appears to occur via autophosphorylation. Taken together, this study demonstrates new structural mechanisms through which two-component systems can transduce environmental signals to impact gene expression and enlightens new potential antimicrobial approach by targeting two-component regulatory systems.
History
Deposition
Jan 27, 2021
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Header (metadata) release
Jun 23, 2021
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Map release
Jun 23, 2021
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Update
Jun 23, 2021
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Current status
Jun 23, 2021
Processing site: PDBj / Status: Released
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