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- EMDB-30624: Mouse Toll-like receptor 3 ectodomain in complex with lncRNA Rmrp... -

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Basic information

Entry
Database: EMDB / ID: EMD-30624
TitleMouse Toll-like receptor 3 ectodomain in complex with lncRNA Rmrp in elongated form
Map data
Sample
  • Complex: Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C-domain of lncRNA Rmrp
    • Protein or peptide: Toll-like receptor 3
    • RNA: RNA component of mitochondrial RNAase P (Rmrp), RNase MRP RNA
Function / homology
Function and homology information


type III interferon production / positive regulation of type III interferon production / response to dsRNA / regulation of dendritic cell cytokine production / inflammatory response to wounding / toll-like receptor 3 signaling pathway / necroptotic signaling pathway / positive regulation of cytokine production involved in inflammatory response / positive regulation of macrophage cytokine production / pattern recognition receptor activity ...type III interferon production / positive regulation of type III interferon production / response to dsRNA / regulation of dendritic cell cytokine production / inflammatory response to wounding / toll-like receptor 3 signaling pathway / necroptotic signaling pathway / positive regulation of cytokine production involved in inflammatory response / positive regulation of macrophage cytokine production / pattern recognition receptor activity / toll-like receptor signaling pathway / response to exogenous dsRNA / cellular response to exogenous dsRNA / positive regulation of interferon-alpha production / positive regulation of type I interferon production / cellular response to interferon-beta / positive regulation of chemokine production / extrinsic apoptotic signaling pathway / JNK cascade / positive regulation of interleukin-12 production / positive regulation of interferon-beta production / positive regulation of interleukin-8 production / positive regulation of JNK cascade / microglial cell activation / response to virus / defense response / cellular response to virus / cellular response to type II interferon / positive regulation of interleukin-6 production / positive regulation of non-canonical NF-kappaB signal transduction / cellular response to mechanical stimulus / male gonad development / positive regulation of angiogenesis / MAPK cascade / positive regulation of type II interferon production / transmembrane signaling receptor activity / cellular response to xenobiotic stimulus / positive regulation of tumor necrosis factor production / double-stranded RNA binding / signaling receptor activity / defense response to virus / positive regulation of canonical NF-kappaB signal transduction / early endosome / endosome membrane / inflammatory response / positive regulation of apoptotic process / innate immune response / endoplasmic reticulum membrane / positive regulation of gene expression / cell surface / positive regulation of transcription by RNA polymerase II / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
Toll-like receptor 3 trans-membrane domain / Toll-like receptor 3 trans-membrane domain / Toll-like receptor / TIR domain / Leucine Rich Repeat / Cysteine-rich flanking region, C-terminal / Leucine rich repeat C-terminal domain / Toll - interleukin 1 - resistance / Leucine-rich repeat, SDS22-like subfamily / TIR domain profile. ...Toll-like receptor 3 trans-membrane domain / Toll-like receptor 3 trans-membrane domain / Toll-like receptor / TIR domain / Leucine Rich Repeat / Cysteine-rich flanking region, C-terminal / Leucine rich repeat C-terminal domain / Toll - interleukin 1 - resistance / Leucine-rich repeat, SDS22-like subfamily / TIR domain profile. / Toll/interleukin-1 receptor homology (TIR) domain / Toll/interleukin-1 receptor homology (TIR) domain superfamily / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat profile. / Leucine-rich repeat / Leucine-rich repeat domain superfamily
Similarity search - Domain/homology
Toll-like receptor 3
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.47 Å
AuthorsLi B / Cao X
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81788101 China
CitationJournal: To Be Published
Title: Endosomal lncRNA Rmrp is required for TLR3 innate sensing by engaging TLR3 dimerization
Authors: Zhang S / Li B / Cao X
History
DepositionOct 15, 2020-
Header (metadata) releaseOct 20, 2021-
Map releaseOct 20, 2021-
UpdateOct 20, 2021-
Current statusOct 20, 2021Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7da7
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_30624.png

Downloads & links

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Map

FileDownload / File: emd_30624.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.82 Å
Density
Contour LevelBy AUTHOR: 0.25 / Movie #1: 0.3
Minimum - Maximum-0.80397487 - 1.6417792
Average (Standard dev.)0.0026856775 (±0.04095878)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 314.88 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.820.820.82
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z314.880314.880314.880
α/β/γ90.00090.00090.000
start NX/NY/NZ-250-250-250
NX/NY/NZ500500500
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.8041.6420.003

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Supplemental data

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Sample components

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Entire : Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C...

EntireName: Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C-domain of lncRNA Rmrp
Components
  • Complex: Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C-domain of lncRNA Rmrp
    • Protein or peptide: Toll-like receptor 3
    • RNA: RNA component of mitochondrial RNAase P (Rmrp), RNase MRP RNA

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Supramolecule #1: Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C...

SupramoleculeName: Cryo-EM structure of mouse Toll-like receptor 3 ectodomain with C-domain of lncRNA Rmrp
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightExperimental: 230 KDa

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Macromolecule #1: Toll-like receptor 3

MacromoleculeName: Toll-like receptor 3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 78.101648 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: HHHHHHENLY FQSQCTVRYN VADCSHLKLT HIPDDLPSNI TVLNLTHNQL RRLPPTNFTR YSQLAILDAG FNSISKLEPE LCQILPLLK VLNLQHNELS QISDQTFVFC TNLTELDLMS NSIHKIKSNP FKNQKNLIKL DLSHNGLSST KLGTGVQLEN L QELLLAKN ...String:
HHHHHHENLY FQSQCTVRYN VADCSHLKLT HIPDDLPSNI TVLNLTHNQL RRLPPTNFTR YSQLAILDAG FNSISKLEPE LCQILPLLK VLNLQHNELS QISDQTFVFC TNLTELDLMS NSIHKIKSNP FKNQKNLIKL DLSHNGLSST KLGTGVQLEN L QELLLAKN KILALRSEEL EFLGNSSLRK LDLSSNPLKE FSPGCFQTIG KLFALLLNNA QLNPHLTEKL CWELSNTSIQ NL SLANNQL LATSESTFSG LKWTNLTQLD LSYNNLHDVG NGSFSYLPSL RYLSLEYNNI QRLSPRSFYG LSNLRYLSLK RAF TKQSVS LASHPNIDDF SFQWLKYLEY LNMDDNNIPS TKSNTFTGLV SLKYLSLSKT FTSLQTLTNE TFVSLAHSPL LTLN LTKNH ISKIANGTFS WLGQLRILDL GLNEIEQKLS GQEWRGLRNI FEIYLSYNKY LQLSTSSFAL VPSLQRLMLR RVALK NVDI SPSPFRPLRN LTILDLSNNN IANINEDLLE GLENLEILDF QHNNLARLWK RANPGGPVNF LKGLSHLHIL NLESNG LDE IPVGVFKNLF ELKSINLGLN NLNKLEPFIF DDQTSLRSLN LQKNLITSVE KDVFGPPFQN LNSLDMRFNP FDCTCES IS WFVNWINQTH TNISELSTHY LCNTPHHYYG FPLKLFDTSS CK

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Macromolecule #2: RNA component of mitochondrial RNAase P (Rmrp), RNase MRP RNA

MacromoleculeName: RNA component of mitochondrial RNAase P (Rmrp), RNase MRP RNA
type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.12452 KDa
SequenceString:
GCUCGCUCUG AAGGCCUGUU UCCUAGGCUA CAUACGAGGG ACAUGUUCCU UAUCCUUUCG CCUAGGGGAA AGUCCCCGGA AGCUCACAU AGUGACGCAG GCAGUGCGAC CUGGCUCGCA CCAACCACAC GGGGCUCAUU CUCAGCGCGG C

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.0 mg/mL
BufferpH: 6
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: OTHER / Pretreatment - Pressure: 101.325 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK IV
DetailsThis sample was mono disperse.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 2.0 µm / Calibrated defocus min: 1.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 165000
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 40 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
TemperatureMin: 78.5 K / Max: 78.5 K
DetailsPreliminary grid screening was preformed manually.
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Number grids imaged: 2 / Number real images: 10617 / Average exposure time: 5.0 sec. / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2545278
CTF correctionSoftware - Name: cryoSPARC (ver. 2.9.1) / Details: CTF correction was done by patch CTF correction.
Startup modelType of model: NONE / Details: The startup models were generated automatically.
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 2.9.1)
Details: The initial angle assignment was generated by Ab-initio reconstruction.
Final 3D classificationNumber classes: 5 / Software - Name: cryoSPARC (ver. 2.9.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 2.9.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.47 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 2.9.1) / Number images used: 108128
DetailsThe selected images were normalized.

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Atomic model buiding 1

Initial modelPDB ID:

3cig


Chain - Chain ID: A / Chain - Residue range: 27-697
RefinementProtocol: RIGID BODY FIT / Overall B value: 97.5 / Target criteria: correlation coefficient
Output model

PDB-7da7:
Mouse Toll-like receptor 3 ectodomain in complex with lncRNA Rmrp in elongated form

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