Japan Agency for Medical Research and Development (AMED)
19am0101117
Japan
Japan Agency for Medical Research and Development (AMED)
17pc0101020
Japan
Citation
Journal: Nat Commun / Year: 2021 Title: Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries. Authors: Akihiro Kawamoto / Tomoko Miyata / Fumiaki Makino / Miki Kinoshita / Tohru Minamino / Katsumi Imada / Takayuki Kato / Keiichi Namba / Abstract: The bacterial flagellar MS ring is a transmembrane complex acting as the core of the flagellar motor and template for flagellar assembly. The C ring attached to the MS ring is involved in torque ...The bacterial flagellar MS ring is a transmembrane complex acting as the core of the flagellar motor and template for flagellar assembly. The C ring attached to the MS ring is involved in torque generation and rotation switch, and a large symmetry mismatch between these two rings has been a long puzzle, especially with respect to their role in motor function. Here, using cryoEM structural analysis of the flagellar basal body and the MS ring formed by full-length FliF from Salmonella enterica, we show that the native MS ring is formed by 34 FliF subunits with no symmetry variation. Symmetry analysis of the C ring shows a variation with a peak at 34-fold, suggesting flexibility in C ring assembly. Finally, our data also indicate that FliF subunits assume two different conformations, contributing differentially to the inner and middle parts of the M ring and thus resulting in 23- and 11-fold subsymmetries in the inner and middle M ring, respectively. The internal core of the M ring, formed by 23 subunits, forms a hole of the right size to accommodate the protein export gate.
History
Deposition
Oct 7, 2020
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Header (metadata) release
May 19, 2021
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Map release
May 19, 2021
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Update
Mar 27, 2024
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Current status
Mar 27, 2024
Processing site: PDBj / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Entire : transmembrane protein complex made of FliF
Entire
Name: transmembrane protein complex made of FliF
Components
Complex: transmembrane protein complex made of FliF
Protein or peptide: Flagellar M-ring protein
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Supramolecule #1: transmembrane protein complex made of FliF
Supramolecule
Name: transmembrane protein complex made of FliF / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all Details: the MS ring formed by full-length FliF from Salmonella enterica serovar Typhimurium
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