+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-30385 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Simplified Alpha-Carboxysome, T=4 | |||||||||
![]() | Simplified Alpha-Carboxysome, T=4 | |||||||||
![]() |
| |||||||||
![]() | Bacterial Microcompartment / Alpha-carboxysome / VIRUS LIKE PARTICLE | |||||||||
Function / homology | ![]() structural constituent of carboxysome shell / carboxysome / carbon fixation Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
![]() | Tan YQ / Ali S | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Structure of a Minimal α-Carboxysome-Derived Shell and Its Utility in Enzyme Stabilization. Authors: Yong Quan Tan / Samson Ali / Bo Xue / Wei Zhe Teo / Lay Hiang Ling / Maybelle Kho Go / Hong Lv / Robert C Robinson / Akihiro Narita / Wen Shan Yew / ![]() ![]() ![]() ![]() Abstract: Bacterial microcompartments are proteinaceous shells that encase specialized metabolic processes in bacteria. Recent advances in simplification of these intricate shells have encouraged ...Bacterial microcompartments are proteinaceous shells that encase specialized metabolic processes in bacteria. Recent advances in simplification of these intricate shells have encouraged bioengineering efforts. Here, we construct minimal shells derived from the α-carboxysome, which we term Cso-shell. Using cryogenic electron microscopy, the atomic-level structures of two shell forms were obtained, reinforcing notions of evolutionarily conserved features in bacterial microcompartment shell architecture. Encapsulation peptide sequences that facilitate loading of heterologous protein cargo within the shells were identified. We further provide a first demonstration in utilizing minimal bacterial microcompartment-derived shells for hosting heterologous enzymes. Cso-shells were found to stabilize enzymatic activities against heat shock, presence of methanol co-solvent, consecutive freeze-thawing, and alkaline environments. This study yields insights into α-carboxysome assembly and advances the utility of synthetic bacterial microcompartments as nanoreactors capable of stabilizing enzymes with varied properties and reaction chemistries. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 49.7 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 11.7 KB 11.7 KB | Display Display | ![]() |
Images | ![]() | 251.5 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ckcMC ![]() 7ckbC ![]() 7dhqC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Simplified Alpha-Carboxysome, T=4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.86 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Simplified Alpha-Carboxysome, T=4
Entire | Name: Simplified Alpha-Carboxysome, T=4 |
---|---|
Components |
|
-Supramolecule #1: Simplified Alpha-Carboxysome, T=4
Supramolecule | Name: Simplified Alpha-Carboxysome, T=4 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 2.3 MDa |
-Macromolecule #1: Unidentified carboxysome polypeptide
Macromolecule | Name: Unidentified carboxysome polypeptide / type: protein_or_peptide / ID: 1 / Number of copies: 60 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 10.17364 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKIMQVEKTL VSTNRIADMG HKPLLVVWEK PGAPRQVAVD AIGCIPGDWV LCVGSSAARE AAGSKSYPSD LTIIGIIDQW NGEGSSWSH PQFEK UniProtKB: Carboxysome shell vertex protein CsoS4A |
-Macromolecule #2: Major carboxysome shell protein 1A
Macromolecule | Name: Major carboxysome shell protein 1A / type: protein_or_peptide / ID: 2 / Number of copies: 180 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() Strain: ATCC 23641 / c2 |
Molecular weight | Theoretical: 9.973478 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MADVTGIALG MIETRGLVPA IEAADAMTKA AEVRLVGRQF VGGGYVTVLV RGETGAVNAA VRAGADACER VGDGLVAAHI IARVHSEVE NILPKAPQA UniProtKB: Major carboxysome shell protein CsoS1A |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.9 |
---|---|
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 298 K |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 64.3 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |