National Natural Science Foundation of China (NSFC)
31971123
中国
National Natural Science Foundation of China (NSFC)
81920108015
中国
National Natural Science Foundation of China (NSFC)
31930059
中国
引用
ジャーナル: Science / 年: 2020 タイトル: Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. 著者: Renhong Yan / Yuanyuan Zhang / Yaning Li / Lu Xia / Yingying Guo / Qiang Zhou / 要旨: Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious ...Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious coronavirus disease 2019 (COVID-19) epidemic. Here, we present cryo-electron microscopy structures of full-length human ACE2 in the presence of the neutral amino acid transporter BAT1 with or without the receptor binding domain (RBD) of the surface spike glycoprotein (S protein) of SARS-CoV-2, both at an overall resolution of 2.9 angstroms, with a local resolution of 3.5 angstroms at the ACE2-RBD interface. The ACE2-BAT1 complex is assembled as a dimer of heterodimers, with the collectrin-like domain of ACE2 mediating homodimerization. The RBD is recognized by the extracellular peptidase domain of ACE2 mainly through polar residues. These findings provide important insights into the molecular basis for coronavirus recognition and infection.
名称: the SARS-coV-2 RBD/ACE2-B0AT1 complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3 詳細: cryo EM map of the complex of 2019-nCoV RBD with the ACE2-B0AT1 complex
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超分子 #2: the SARS-coV-2 RBD
超分子
名称: the SARS-coV-2 RBD / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #3 詳細: cryo EM map of the complex of 2019-nCoV RBD with the ACE2-B0AT1 complex, focused refined on 2019-nCoV RBD
由来(天然)
生物種: Severe acute respiratory syndrome coronavirus 2 (ウイルス)
組換発現
生物種: Homo sapiens (ヒト)
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超分子 #3: ACE2-B0AT1
超分子
名称: ACE2-B0AT1 / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #1-#2 詳細: cryo EM map of the complex of 2019-nCoV RBD with the ACE2-B0AT1 complex, focused refined on ACE2-B0AT1