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Open data
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Basic information
Entry | Database: PDB / ID: 5wel | ||||||||||||||||||||||||||||||||
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Title | GluA2 bound to antagonist ZK and GSG1L in digitonin, state 2 | ||||||||||||||||||||||||||||||||
![]() | Chimera of Glutamate receptor 2, Germ cell-specific gene 1-like protein | ||||||||||||||||||||||||||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||
Specimen source | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||
![]() | Twomey, E.C. / Yelshanskaya, M.V. / Grassucci, R.A. / Frank, J. / Sobolevsky, A.I. | ||||||||||||||||||||||||||||||||
![]() | Journal: Nature / Year: 2017 Title: Channel opening and gating mechanism in AMPA-subtype glutamate receptors. ![]() | ||||||||||||||||||||||||||||||||
Validation Report | ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||
Date | Deposition: Jul 10, 2017 / Release: Aug 2, 2017
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmcif format | ![]() ![]() |
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PDB format | ![]() ![]() |
PDBML Plus | ![]() |
Others | ![]() |
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Links
-Related structure data
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein/peptide | Mass: 117471.211 Da / Num. of mol.: 4 Fragment: UNP P19491 residues 25-847, UNP D3Z7H4 residues 2-238 linked via LINKER GTG Mutation: N241E, V382L, G384E, N385D, N392Q, V1151L Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() Gene: Gria2, Glur2 ![]() ![]() ![]() #2: Chemical | ChemComp-ZK1 / {[ #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / Reconstruction method: ![]() |
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Sample preparation
Component | Name: GluA2 bound to antagonist ZK and GSG1L in digitonin, state 2 Type: COMPLEX / Entity ID: 1 / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Conc.: 5 mg/ml Details: GluA2 bound to antagonist ZK and GSG1L in digitonin, state 2 Embedding applied: NO / Shadowing applied: NO / Staining applied ![]() ![]() |
Specimen support | Details: Gold-gold grids, hydrogen and oxygen glow discharge (20s, 10 watts, 6.4 sccm H2, 27.5 sccm O2) Grid material: GOLD / Grid mesh size: 200 / Grid type: C-flat-1.2/1.3 |
Vitrification![]() | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 295 kelvins |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Microscopy | Microscope model: FEI POLARA 300 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 67 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
Image scans | Movie frames/image: 40 |
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Processing
Software | Name: PHENIX / Version: 1.11.1_2575 / Classification: refinement | ||||||||||||||||||||||||
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Symmetry | Point symmetry: C2 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 41926 / Symmetry type: POINT | ||||||||||||||||||||||||
Atomic model building | Ref space: REAL | ||||||||||||||||||||||||
Refine LS restraints |
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