EMDB-29260: Human nucleolar pre-60S ribosomal subunit (State E) - Composite map

基本情報

登録情報

データベース: EMDB / ID: EMD-29260

• タイトル: Human nucleolar pre-60S ribosomal subunit (State E) - Composite map

試料

• 複合体: Human nucleolar pre-60S ribosomal subunit (State E)
  • タンパク質・ペプチド: x 50種
  • RNA: x 3種
• リガンド: x 5種

• キーワード: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

機能・相同性

分子機能:

RNA 2'-O-methyltransferase activity / Noc1p-Noc2p complex / Noc2p-Noc3p complex / RNA metabolic process / TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / rRNA (uridine-2'-O-)-methyltransferase activity / rRNA (guanine) methyltransferase activity / rRNA (cytosine-C5-)-methyltransferase activity / dendrite extension / preribosome binding / regulation of cellular senescence / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / PeBoW complex / RNA methylation / positive regulation of protein sumoylation / miRNA-mediated post-transcriptional gene silencing / stem cell division / miRNA-mediated gene silencing by inhibition of translation / positive regulation of telomere maintenance / rRNA base methylation / ribosomal protein import into nucleus / negative regulation of formation of translation preinitiation complex / blastocyst formation / protein localization to nucleolus / TORC2 complex binding / negative regulation of B cell apoptotic process / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methylation / GAIT complex / regulation of glycolytic process / maturation of 5.8S rRNA / A band / regulation of reactive oxygen species metabolic process / rRNA modification in the nucleus and cytosol / regulation of G1 to G0 transition / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / preribosome, small subunit precursor / protein-DNA complex disassembly / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / negative regulation of ubiquitin protein ligase activity / mitotic metaphase chromosome alignment / stem cell population maintenance / regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of cell-cell adhesion / positive regulation of dendritic spine development / homeostatic process / rRNA metabolic process / G1 to G0 transition / negative regulation of DNA replication / macrophage chemotaxis / lung morphogenesis / fat cell differentiation / Protein hydroxylation / ribosomal large subunit binding / Peptide chain elongation / rRNA transcription / preribosome, large subunit precursor / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / nuclear-transcribed mRNA catabolic process / Formation of a pool of free 40S subunits / blastocyst development / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / nucleosome binding / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / DNA replication initiation / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / cellular response to interleukin-4 / hematopoietic progenitor cell differentiation / negative regulation of intrinsic apoptotic signaling pathway / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / RNA processing / rough endoplasmic reticulum / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / MDM2/MDM4 family protein binding / negative regulation of protein ubiquitination / maturation of LSU-rRNA / estrogen receptor signaling pathway / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly

ドメイン・相同性:

P120R repeat / P120R (NUC006) repeat / DBP10, C-terminal / DDX54/DBP10 family / DBP10CT (NUC160) domain / DBP10CT (NUC160) domain / Methyltr_RsmF/B-like, ferredoxin-like domain / Nucleolar complex protein 2 / Noc2p family / Nucleolar complex-associated protein 3, N-terminal / Nucleolar complex-associated protein 3 / Nucleolar complex-associated protein / MKI67 FHA domain-interacting nucleolar phosphoprotein, FHA Ki67 binding / FHA Ki67 binding domain of hNIFK / Ribosome biogenesis factor, NIP7 / RNA (C5-cytosine) methyltransferase, NOP2 / UPF0113, pre-PUA domain / UPF0113 Pre-PUA domain / UPF0113 PUA domain / UPF0113, PUA domain / Nop2p / Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site / NOL1/NOP2/sun family signature. / Learning associated protein 18-like / LLP homolog / Ribosomal RNA methyltransferase, SPB1-like, C-terminal / Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 / AdoMet-dependent rRNA methyltransferase SPB1-like / Spb1 C-terminal domain / Ribosomal RNA methyltransferase Spb1, DUF3381 / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / : / Ribosomal RNA large subunit methyltransferase E / Ribosome biogenesis protein BRX1 / : / CCAAT-binding factor / DDX18/Has1, DEAD-box helicase domain / CBF/Mak21 family / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein Nop16 / Domain of unknown function DUF4217 / Domain of unknown function (DUF4217) / DUF4217 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / BOP1NT (NUC169) domain / BOP1NT (NUC169) domain / SAM-dependent methyltransferase RsmB/NOP2-type / RNA (C5-cytosine) methyltransferase / : / 16S rRNA methyltransferase RsmB/F / SAM-dependent MTase RsmB/NOP-type domain profile. / Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase / PUA domain / Guanine nucleotide-binding protein-like 3, N-terminal domain / GNL3L/Grn1 putative GTPase / PUA domain superfamily / PUA domain profile. / : / Pescadillo / Pescadillo N-terminus / Ribosomal protein L24e / Ribosomal biogenesis NSA2 family / Ribosome assembly factor Mrt4 / : / BRCT domain, a BRCA1 C-terminus domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Brix domain / Brix domain / Brix domain profile. / Brix / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / 50S ribosome-binding GTPase / DEAD-box subfamily ATP-dependent helicases signature. / RNA helicase, DEAD-box type, Q motif / ATP-dependent RNA helicase DEAD-box, conserved site / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / DEAD-box RNA helicase Q motif profile. / GTP binding domain / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / PUA-like superfamily / Ribosomal protein L23 / 50S ribosomal protein L10, insertion domain superfamily

構成要素:

Pescadillo homolog / Ribosomal L1 domain-containing protein 1 / Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / 28S rRNA (cytosine(4447)-C(5))-methyltransferase / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Ribosome biogenesis protein BOP1 / Ribosome biogenesis regulatory protein homolog / Ribosomal protein uL30-like / pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / ATP-dependent RNA helicase DDX54 / Ribosome biogenesis protein BRX1 homolog / Nucleolar complex protein 3 homolog / Probable rRNA-processing protein EBP2 / Protein LLP homolog / Guanine nucleotide-binding protein-like 3 / MKI67 FHA domain-interacting nucleolar phosphoprotein / GTP-binding protein 4 / ATP-dependent RNA helicase DDX18 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog / 60S ribosome subunit biogenesis protein NIP7 homolog / Nucleolar protein 16 / Nucleolar complex protein 2 homolog / Large ribosomal subunit protein eL36

• 生物種: Homo sapiens (ヒト) / human (ヒト)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.59 Å
• データ登録者: Vanden Broeck A / Klinge S

資金援助

European Union, 米国, 2件

Organization	Grant number	国
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	米国

• 引用: ジャーナル: Science / 年: 2023; タイトル: Principles of human pre-60 biogenesis.; 著者: Arnaud Vanden Broeck / Sebastian Klinge / ; 要旨: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

履歴

登録	2022年12月21日	-
ヘッダ(付随情報) 公開	2023年7月12日	-
マップ公開	2023年7月12日	-
更新	2023年7月19日	-
現状	2023年7月19日	処理サイト: RCSB / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_29260.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• ボクセルのサイズ: X=Y=Z: 1.072 Å

密度

表面レベル	登録者による: 0.85
最小 - 最大	-0.0083639305 - 9.181450999999999
平均 (標準偏差)	0.044719234 (±0.17788212)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 480 480 480 Spacing 480 480 480
セル	A=B=C: 514.56 Å α=β=γ: 90.0 °

添付データ

マスク #1

• ファイル: emd_29260_msk_1.map

ハーフマップ: #2

• ファイル: emd_29260_half_map_1.map

ハーフマップ: #1

• ファイル: emd_29260_half_map_2.map

試料の構成要素

全体 : Human nucleolar pre-60S ribosomal subunit (State E)

• 全体: 名称: Human nucleolar pre-60S ribosomal subunit (State E)

要素

• 複合体: Human nucleolar pre-60S ribosomal subunit (State E)
  • タンパク質・ペプチド: 60S ribosomal protein L12
  • タンパク質・ペプチド: 60S ribosomal protein L10a
  • RNA: 5.8S rRNA
  • RNA: ITS2 rRNA
  • RNA: 28S rRNA
  • タンパク質・ペプチド: 60S ribosomal protein L13
  • タンパク質・ペプチド: 60S ribosomal protein L13a
  • タンパク質・ペプチド: 60S ribosomal protein L14
  • タンパク質・ペプチド: 60S ribosomal protein L15
  • タンパク質・ペプチド: 60S ribosomal protein L17
  • タンパク質・ペプチド: 60S ribosomal protein L18
  • タンパク質・ペプチド: 60S ribosomal protein L18a
  • タンパク質・ペプチド: 60S ribosomal protein L21
  • タンパク質・ペプチド: 60S ribosomal protein L23
  • タンパク質・ペプチド: 60S ribosomal protein L23a
  • タンパク質・ペプチド: 60S ribosomal protein L26
  • タンパク質・ペプチド: 60S ribosomal protein L27a
  • タンパク質・ペプチド: 60S ribosomal protein L28
  • タンパク質・ペプチド: 60S ribosomal protein L3
  • タンパク質・ペプチド: 60S ribosomal protein L31
  • タンパク質・ペプチド: 60S ribosomal protein L32
  • タンパク質・ペプチド: 60S ribosomal protein L35
  • タンパク質・ペプチド: 60S ribosomal protein L35a
  • タンパク質・ペプチド: 60S ribosomal protein L36
  • タンパク質・ペプチド: 60S ribosomal protein L37
  • タンパク質・ペプチド: Nucleolar complex protein 2 homolog
  • タンパク質・ペプチド: Guanine nucleotide-binding protein-like 3
  • タンパク質・ペプチド: Ribosome biogenesis protein NSA2 homolog
  • タンパク質・ペプチド: 60S ribosome subunit biogenesis protein NIP7 homolog
  • タンパク質・ペプチド: ATP-dependent RNA helicase DDX54
  • タンパク質・ペプチド: Protein LLP homolog
  • タンパク質・ペプチド: 60S ribosomal protein L4
  • タンパク質・ペプチド: 60S ribosomal protein L6
  • タンパク質・ペプチド: 60S ribosomal protein L7
  • タンパク質・ペプチド: 60S ribosomal protein L7a
  • タンパク質・ペプチド: 60S ribosomal protein L9
  • タンパク質・ペプチド: MKI67 FHA domain-interacting nucleolar phosphoprotein
  • タンパク質・ペプチド: 60S ribosomal protein L7-like 1
  • タンパク質・ペプチド: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
  • タンパク質・ペプチド: Eukaryotic translation initiation factor 6
  • タンパク質・ペプチド: Ribosomal L1 domain-containing protein 1
  • タンパク質・ペプチド: Pescadillo homolog
  • タンパク質・ペプチド: Probable rRNA-processing protein EBP2
  • タンパク質・ペプチド: Ribosome biogenesis protein BRX1 homolog
  • タンパク質・ペプチド: mRNA turnover protein 4 homolog
  • タンパク質・ペプチド: GTP-binding protein 4
  • タンパク質・ペプチド: Ribosome biogenesis protein BOP1
  • タンパク質・ペプチド: Ribosome biogenesis regulatory protein homolog
  • タンパク質・ペプチド: Nucleolar complex protein 3 homolog
  • タンパク質・ペプチド: Probable ribosome biogenesis protein RLP24
  • タンパク質・ペプチド: ATP-dependent RNA helicase DDX18
  • タンパク質・ペプチド: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
  • タンパク質・ペプチド: Nucleolar protein 16
• リガンド: MAGNESIUM ION
• リガンド: ZINC ION
• リガンド: ADENOSINE-5'-DIPHOSPHATE
• リガンド: GUANOSINE-5'-DIPHOSPHATE
• リガンド: POTASSIUM ION

超分子 #1: Human nucleolar pre-60S ribosomal subunit (State E)

• 超分子: 名称: Human nucleolar pre-60S ribosomal subunit (State E) / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#53
• 由来(天然): 生物種: Homo sapiens (ヒト) / 株: HEK293F

分子 #1: 60S ribosomal protein L12

• 分子: 名称: 60S ribosomal protein L12 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.847619 KDa

配列

文字列:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

分子 #2: 60S ribosomal protein L10a

• 分子: 名称: 60S ribosomal protein L10a / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 24.879422 KDa

配列

文字列:

MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

UniProtKB: Large ribosomal subunit protein uL1

分子 #6: 60S ribosomal protein L13

• 分子: 名称: 60S ribosomal protein L13 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 24.321682 KDa

配列

文字列:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

分子 #7: 60S ribosomal protein L13a

• 分子: 名称: 60S ribosomal protein L13a / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 23.633412 KDa

配列

文字列:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

分子 #8: 60S ribosomal protein L14

• 分子: 名称: 60S ribosomal protein L14 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 23.485016 KDa

配列

文字列:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

分子 #9: 60S ribosomal protein L15

• 分子: 名称: 60S ribosomal protein L15 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 24.207285 KDa

配列

文字列:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

分子 #10: 60S ribosomal protein L17

• 分子: 名称: 60S ribosomal protein L17 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.44317 KDa

配列

文字列:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

分子 #11: 60S ribosomal protein L18

• 分子: 名称: 60S ribosomal protein L18 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.687676 KDa

配列

文字列:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

分子 #12: 60S ribosomal protein L18a

• 分子: 名称: 60S ribosomal protein L18a / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 20.808514 KDa

配列

文字列:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

分子 #13: 60S ribosomal protein L21

• 分子: 名称: 60S ribosomal protein L21 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 18.609988 KDa

配列

文字列:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

分子 #14: 60S ribosomal protein L23

• 分子: 名称: 60S ribosomal protein L23 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.892505 KDa

配列

文字列:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

分子 #15: 60S ribosomal protein L23a

• 分子: 名称: 60S ribosomal protein L23a / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.740193 KDa

配列

文字列:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

分子 #16: 60S ribosomal protein L26

• 分子: 名称: 60S ribosomal protein L26 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.303363 KDa

配列

文字列:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

分子 #17: 60S ribosomal protein L27a

• 分子: 名称: 60S ribosomal protein L27a / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 16.604535 KDa

配列

文字列:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

分子 #18: 60S ribosomal protein L28

• 分子: 名称: 60S ribosomal protein L28 / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.784622 KDa

配列

文字列:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

分子 #19: 60S ribosomal protein L3

• 分子: 名称: 60S ribosomal protein L3 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 46.224133 KDa

配列

文字列:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

分子 #20: 60S ribosomal protein L31

• 分子: 名称: 60S ribosomal protein L31 / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.494938 KDa

配列

文字列:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

分子 #21: 60S ribosomal protein L32

• 分子: 名称: 60S ribosomal protein L32 / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.898932 KDa

配列

文字列:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

分子 #22: 60S ribosomal protein L35

• 分子: 名称: 60S ribosomal protein L35 / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.593624 KDa

配列

文字列:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

分子 #23: 60S ribosomal protein L35a

• 分子: 名称: 60S ribosomal protein L35a / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.564743 KDa

配列

文字列:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

分子 #24: 60S ribosomal protein L36

• 分子: 名称: 60S ribosomal protein L36 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.290859 KDa

配列

文字列:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

分子 #25: 60S ribosomal protein L37

• 分子: 名称: 60S ribosomal protein L37 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 11.111032 KDa

配列

文字列:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

分子 #26: Nucleolar complex protein 2 homolog

• 分子: 名称: Nucleolar complex protein 2 homolog / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 85.028359 KDa

配列

文字列:

MAAAGSRKRR LAELTVDEFL ASGFDSESES ESENSPQAET REAREAARSP DKPGGSPSAS RRKGRASEHK DQLSRLKDRD PEFYKFLQE NDQSLLNFSD SDSSEEEEGP FHSLPDVLEE ASEEEDGAEE GEDGDRVPRG LKGKKNSVPV TVAMVERWKQ A AKQRLTPK LFHEVVQAFR AAVATTRGDQ ESAEANKFQV TDSAAFNALV TFCIRDLIGC LQKLLFGKVA KDSSRMLQPS SS PLWGKLR VDIKAYLGSA IQLVSCLSET TVLAAVLRHI SVLVPCFLTF PKQCRMLLKR MVIVWSTGEE SLRVLAFLVL SRV CRHKKD TFLGPVLKQM YITYVRNCKF TSPGALPFIS FMQWTLTELL ALEPGVAYQH AFLYIRQLAI HLRNAMTTRK KETY QSVYN WQYVHCLFLW CRVLSTAGPS EALQPLVYPL AQVIIGCIKL IPTARFYPLR MHCIRALTLL SGSSGAFIPV LPFIL EMFQ QVDFNRKPGR MSSKPINFSV ILKLSNVNLQ EKAYRDGLVE QLYDLTLEYL HSQAHCIGFP ELVLPVVLQL KSFLRE CKV ANYCRQVQQL LGKVQENSAY ICSRRQRVSF GVSEQQAVEA WEKLTREEGT PLTLYYSHWR KLRDREIQLE ISGKERL ED LNFPEIKRRK MADRKDEDRK QFKDLFDLNS SEEDDTEGFS ERGILRPLST RHGVEDDEED EEEGEEDSSN SEDGDPDA E AGLAPGELQQ LAQGPEDELE DLQLSEDD

UniProtKB: Nucleolar complex protein 2 homolog

分子 #27: Guanine nucleotide-binding protein-like 3

• 分子: 名称: Guanine nucleotide-binding protein-like 3 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 62.098242 KDa

配列

文字列:

MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKK RKLETNPDIK PSNVEPMEKE FGLCKTENKA KSGKQNSKKL YCQELKKVIE ASDVVLEVLD ARDPLGCRCP Q VEEAIVQS GQKKLVLILN KSDLVPKENL ESWLNYLKKE LPTVVFRAST KPKDKGKITK RVKAKKNAAP FRSEVCFGKE GL WKLLGGF QETCSKAIRV GVIGFPNVGK SSIINSLKQE QMCNVGVSMG LTRSMQVVPL DKQITIIDSP SFIVSPLNSS SAL ALRSPA SIEVVKPMEA ASAILSQADA RQVVLKYTVP GYRNSLEFFT VLAQRRGMHQ KGGIPNVEGA AKLLWSEWTG ASLA YYCHP PTSWTPPPYF NESIVVDMKS GFNLEELEKN NAQSIRAIKG PHLANSILFQ SSGLTNGIIE EKDIHEELPK RKERK QEER EDDKDSDQET VDEEVDENSS GMFAAEETGE ALSEETTAGE QSTRSFILDK IIEEDDAYDF STDYV

UniProtKB: Guanine nucleotide-binding protein-like 3

分子 #28: Ribosome biogenesis protein NSA2 homolog

• 分子: 名称: Ribosome biogenesis protein NSA2 homolog / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 30.136703 KDa

配列

文字列:

MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

分子 #29: 60S ribosome subunit biogenesis protein NIP7 homolog

• 分子: 名称: 60S ribosome subunit biogenesis protein NIP7 homolog; タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 20.491633 KDa

配列

文字列:

MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCF GKFTKTHKFR LHVTALDYL APYAKYKVWI KPGAEQSFLY GNHVLKSGLG RITENTSQYQ GVVVYSMADI PLGFGVAAKS TQDCRKVDPM A IVVFHQAD IGEYVRHEET LT

UniProtKB: 60S ribosome subunit biogenesis protein NIP7 homolog

分子 #30: ATP-dependent RNA helicase DDX54

• 分子: 名称: ATP-dependent RNA helicase DDX54 / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 98.769164 KDa

配列

文字列:

MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG PGRPLPTFPT SECTSDVEPD TREMVRAQN KKKKKSGGFQ SMGLSYPVFK GIMKKGYKVP TPIQRKTIPV ILDGKDVVAM ARTGSGKTAC FLLPMFERLK T HSAQTGAR ALILSPTREL ALQTLKFTKE LGKFTGLKTA LILGGDRMED QFAALHENPD IIIATPGRLV HVAVEMSLKL QS VEYVVFD EADRLFEMGF AEQLQEIIAR LPGGHQTVLF SATLPKLLVE FARAGLTEPV LIRLDVDTKL NEQLKTSFFL VRE DTKAAV LLHLLHNVVR PQDQTVVFVA TKHHAEYLTE LLTTQRVSCA HIYSALDPTA RKINLAKFTL GKCSTLIVTD LAAR GLDIP LLDNVINYSF PAKGKLFLHR VGRVARAGRS GTAYSLVAPD EIPYLLDLHL FLGRSLTLAR PLKEPSGVAG VDGML GRVP QSVVDEEDSG LQSTLEASLE LRGLARVADN AQQQYVRSRP APSPESIKRA KEMDLVGLGL HPLFSSRFEE EELQRL RLV DSIKNYRSRA TIFEINASSR DLCSQVMRAK RQKDRKAIAR FQQGQQGRQE QQEGPVGPAP SRPALQEKQP EKEEEEE AG ESVEDIFSEV VGRKRQRSGP NRGAKRRREE ARQRDQEFYI PYRPKDFDSE RGLSISGEGG AFEQQAAGAV LDLMGDEA Q NLTRGRQQLK WDRKKKRFVG QSGQEDKKKI KTESGRYISS SYKRDLYQKW KQKQKIDDRD SDEEGASDRR GPERRGGKR DRGQGASRPH APGTPAGRVR PELKTKQQIL KQRRRAQKLH FLQRGGLKQL SARNRRRVQE LQQGAFGRGA RSKKGKMRKR M

UniProtKB: ATP-dependent RNA helicase DDX54

分子 #31: Protein LLP homolog

• 分子: 名称: Protein LLP homolog / タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.268361 KDa

配列

文字列:

MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

分子 #32: 60S ribosomal protein L4

• 分子: 名称: 60S ribosomal protein L4 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 47.804621 KDa

配列

文字列:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

分子 #33: 60S ribosomal protein L6

• 分子: 名称: 60S ribosomal protein L6 / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 32.810176 KDa

配列

文字列:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

分子 #34: 60S ribosomal protein L7

• 分子: 名称: 60S ribosomal protein L7 / タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 29.290973 KDa

配列

文字列:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

分子 #35: 60S ribosomal protein L7a

• 分子: 名称: 60S ribosomal protein L7a / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 30.061785 KDa

配列

文字列:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

分子 #36: 60S ribosomal protein L9

• 分子: 名称: 60S ribosomal protein L9 / タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.899471 KDa

配列

文字列:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

分子 #37: MKI67 FHA domain-interacting nucleolar phosphoprotein

• 分子: 名称: MKI67 FHA domain-interacting nucleolar phosphoprotein; タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 34.285309 KDa

配列

文字列:

MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYA FVEFESEDVA KIVAETMNNY LFGERLLECH FMPPEKVHKE LFKDWNIPFK QPSYPSVKRY NRNRTLTQKL R MEERFKKK ERLLRKKLAK KGIDYDFPSL ILQKTESISK TNRQTSTKGQ VLRKKKKKVS GTLDTPEKTV DSQGPTPVCT PT FLERRKS QVAELNDDDK DDEIVFKQPI SCVKEEIQET QTPTHSRKKR RRSSNQ

UniProtKB: MKI67 FHA domain-interacting nucleolar phosphoprotein

分子 #38: 60S ribosomal protein L7-like 1

• 分子: 名称: 60S ribosomal protein L7-like 1 / タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 29.730354 KDa

配列

文字列:

MISSCTTRKM AEQEQRKIPL VPENLLKKRK AYQALKATQA KQALLAKKEQ KKGKGLRFKR LESFLHDSWR QKRDKVRLRR LEVKPHALE LPDKHSLAFV VRIERIDGVS LLVQRTIARL RLKKIFSGVF VKVTPQNLKM LRIVEPYVTW GFPNLKSVRE L ILKRGQAK VKNKTIPLTD NTVIEEHLGK FGVICLEDLI HEIAFPGKHF QEISWFLCPF HLSVARHATK NRVGFLKEMG TP GYRGERI NQLIRQLN

UniProtKB: Ribosomal protein uL30-like

分子 #39: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

• 分子: 名称: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO; EC番号: 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 96.726875 KDa

配列

文字列:

MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD LCAAPGGWLQ VAAKFMPVSS LIVGVDLVPI KPLPNVVTL QQDITTERCR QALRKELKTW KVDVVLNDGA PNVGASWVHD AYSQAHLTLM ALRLACDFLA RGGSFITKVF R SRDYQPLL WIFQQLFRRV QATKPQASRH ESAEIFVVCQ GFLAPDKVDS KFFDPKFAFK EVEVQAKTVT ELVTKKKPKA EG YAEGDLT LYHRTSVTDF LRAANPVDFL SKASEIMVDD EELAQHPATT EDIRVCCQDI RVLGRKELRS LLNWRTKLRR YVA KKLKEQ AKALDISLSS GEEDEGDEED STAGTTKQPS KEEEEEEEEE QLNQTLAEMK AQEVAELKRK KKKLLREQRK QRER VELKM DLPGVSIADE GETGMFSLST IRGHQLLEEV TQGDMSAADT FLSDLPRDDI YVSDVEDDGD DTSLDSDLDP EELAG VRGH QGLRDQKRMR LTEVQDDKEE EEEENPLLVP LEEKAVLQEE QANLWFSKGS FAGIEDDADE ALEISQAQLL FENRRK GRQ QQQKQQLPQT PPSCLKTEIM SPLYQDEAPK GTEASSGTEA ATGLEGEEKD GISDSDSSTS SEEEESWEPL RGKKRSR GP KSDDDGFEIV PIEDPAKHRI LDPEGLALGA VIASSKKAKR DLIDNSFNRY TFNEDEGELP EWFVQEEKQH RIRQLPVG K KEVEHYRKRW REINARPIKK VAEAKARKKR RMLKRLEQTR KKAEAVVNTV DISEREKVAQ LRSLYKKAGL GKEKRHVTY VVAKKGVGRK VRRPAGVRGH FKVVDSRMKK DQRAQQRKEQ KKKHKRK

UniProtKB: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

分子 #40: Eukaryotic translation initiation factor 6

• 分子: 名称: Eukaryotic translation initiation factor 6 / タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 26.62001 KDa

配列

文字列:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

分子 #41: Ribosomal L1 domain-containing protein 1

• 分子: 名称: Ribosomal L1 domain-containing protein 1 / タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 55.08975 KDa

配列

文字列:

MEDSASASLS SAAATGTSTS TPAAPTARKQ LDKEQVRKAV DALLTHCKSR KNNYGLLLNE NESLFLMVVL WKIPSKELRV RLTLPHSIR SDSEDICLFT KDEPNSTPEK TEQFYRKLLN KHGIKTVSQI ISLQTLKKEY KSYEAKLRLL SSFDFFLTDA R IRRLLPSL IGRHFYQRKK VPVSVNLLSK NLSREINDCI GGTVLNISKS GSCSAIRIGH VGMQIEHIIE NIVAVTKGLS EK LPEKWES VKLLFVKTEK SAALPIFSSF VSNWDEATKR SLLNKKKKEA RRKRRERNFE KQKERKKKRQ QARKTASVLS KDD VAPESG DTTVKKPESK KEQTPEHGKK KRGRGKAQVK ATNESEDEIP QLVPIGKKTP ANEKVEIQKH ATGKKSPAKS PNPS TPRGK KRKALPASET PKAAESETPG KSPEKKPKIK EEAVKEKSPS LGKKDARQTP KKPEAKFFTT PSKSVRKASH TPKKW PKKP KVPQST

UniProtKB: Ribosomal L1 domain-containing protein 1

分子 #42: Pescadillo homolog

• 分子: 名称: Pescadillo homolog / タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 68.114727 KDa

配列

文字列:

MGGLEKKKYE RGSATNYITR NKARKKLQLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA ARTFYLIKDI RFLLHEPIVN KFREYKVFV RKLRKAYGKS EWNTVERLKD NKPNYKLDHI IKERYPTFID ALRDLDDALS MCFLFSTFPR TGKCHVQTIQ L CRRLTVEF MHYIIAARAL RKVFLSIKGI YYQAEVLGQP IVWITPYAFS HDHPTDVDYR VMATFTEFYT TLLGFVNFRL YQ LLNLHYP PKLEGQAQAE AKAGEGTYAL DSESCMEKLA ALSASLARVV VPATEEEAEV DEFPTDGEMS AQEEDRRKEL EAQ EKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFD SVNAR LLLPVAEYFS GVQLPPHLSP FVTEKEGDYV PPEKLKLLAL QRGEDPGNLN ESEEEEEEDD NNEGDGDEEG ENEEE EEDA EAGSEKEEEA RLAALEEQRM EGKKPRVMAG TLKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG KRRKIR EAN KLAEKRKAHD EAVRSEKKAK KARPE

UniProtKB: Pescadillo homolog

分子 #43: Probable rRNA-processing protein EBP2

• 分子: 名称: Probable rRNA-processing protein EBP2 / タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 34.925145 KDa

配列

文字列:

MDTPPLSDSE SESDESLVTD RELQDAFSRG LLKPGLNVVL EGPKKAVNDV NGLKQCLAEF KRDLEWVERL DVTLGPVPEI GGSEAPAPQ NKDQKAVDPE DDFQREMSFY RQAQAAVLAV LPRLHQLKVP TKRPTDYFAE MAKSDLQMQK IRQKLQTKQA A MERSEKAK QLRALRKYGK KVQTEVLQKR QQEKAHMMNA IKKYQKGFSD KLDFLEGDQK PLAQRKKAGA KGQQMRKGPS AK RRYKNQK FGFGGKKKGS KWNTRESYDD VSSFRAKTAH GRGLKRPGKK GSNKRPGKRT REKMKNRTH

UniProtKB: Probable rRNA-processing protein EBP2

分子 #44: Ribosome biogenesis protein BRX1 homolog

• 分子: 名称: Ribosome biogenesis protein BRX1 homolog / タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 41.483516 KDa

配列

文字列:

MAATKRKRRG GFAVQAKKPK RNEIDAEPPA KRHATAEEVE EEERDRIPGP VCKGKWKNKE RILIFSSRGI NFRTRHLMQD LRMLMPHSK ADTKMDRKDK LFVINEVCEM KNCNKCIYFE AKKKQDLYMW LSNSPHGPSA KFLVQNIHTL AELKMTGNCL K GSRPLLSF DPAFDELPHY ALLKELLIQI FSTPRYHPKS QPFVDHVFTF TILDNRIWFR NFQIIEEDAA LVEIGPRFVL NL IKIFQGS FGGPTLYENP HYQSPNMHRR VIRSITAAKY REKQQVKDVQ KLRKKEPKTL LPHDPTADVF VTPAEEKPIE IQW VKPEPK VDLKARKKRI YKRQRKMKQR MDSGKTK

UniProtKB: Ribosome biogenesis protein BRX1 homolog

分子 #45: mRNA turnover protein 4 homolog

• 分子: 名称: mRNA turnover protein 4 homolog / タイプ: protein_or_peptide / ID: 45 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 27.602535 KDa

配列

文字列:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

分子 #46: GTP-binding protein 4

• 分子: 名称: GTP-binding protein 4 / タイプ: protein_or_peptide / ID: 46 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 74.10782 KDa

配列

文字列:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

分子 #47: Ribosome biogenesis protein BOP1

• 分子: 名称: Ribosome biogenesis protein BOP1 / タイプ: protein_or_peptide / ID: 47 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 83.901469 KDa

配列

文字列:

MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF SGLEDSGSDS SEDDDEGDEE GEDGALDDE GHSGIKKTTE EQVQASTPCP RTEMASARIG DEYAED(SEP)(SEP)DE EDIRNTVGNV PLEWYDDFPH VGY DLDGRR IYKPLRTRDE LDQFLDKMDD PDYWRTVQDP MTGRDLRLTD EQVALVRRLQ SGQFGDVGFN PYEPAVDFFS GDVM IHPVT NRPADKRSFI PSLVEKEKVS RMVHAIKMGW IQPRRPRDPT PSFYDLWAQE DPNAVLGRHK MHVPAPKLAL PGHAE SYNP PPEYLLSEEE RLAWEQQEPG ERKLSFLPRK FPSLRAVPAY GRFIQERFER CLDLYLCPRQ RKMRVNVDPE DLIPKL PRP RDLQPFPTCQ ALVYRGHSDL VRCLSVSPGG QWLVSGSDDG SLRLWEVATA RCVRTVPVGG VVKSVAWNPS PAVCLVA AA VEDSVLLLNP ALGDRLVAGS TDQLLSAFVP PEEPPLQPAR WLEASEEERQ VGLRLRICHG KPVTQVTWHG RGDYLAVV L ATQGHTQVLI HQLSRRRSQS PFRRSHGQVQ RVAFHPARPF LLVASQRSVR LYHLLRQELT KKLMPNCKWV SSLAVHPAG DNVICGSYDS KLVWFDLDLS TKPYRMLRHH KKALRAVAFH PRYPLFASGS DDGSVIVCHG MVYNDLLQNP LLVPVKVLKG HVLTRDLGV LDVIFHPTQP WVFSSGADGT VRLFT

UniProtKB: Ribosome biogenesis protein BOP1

分子 #48: Ribosome biogenesis regulatory protein homolog

• 分子: 名称: Ribosome biogenesis regulatory protein homolog / タイプ: protein_or_peptide / ID: 48 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 41.278523 KDa

配列

文字列:

MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE DQFAKRIQAK KERVAKNELN RLRNLARAHK MQLPSAAGLH PTGHQSKEEL GRAMQVAKVS TASVGRFQER LP KEKVPRG SGKKRKFQPL FGDFAAEKKN QLELLRVMNS KKPQLDVTRA TNKQMREEDQ EEAAKRRKMS QKGKRKGGRQ GPG GKRKGG PPSQGGKRKG GLGGKMNSGP PGLGGKRKGG QRPGGKRRK

UniProtKB: Ribosome biogenesis regulatory protein homolog

分子 #49: Nucleolar complex protein 3 homolog

• 分子: 名称: Nucleolar complex protein 3 homolog / タイプ: protein_or_peptide / ID: 49 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 92.709945 KDa

配列

文字列:

MKARRNKKQI PSFRKLIKTS KVKLENKLKN KQFKQQSTLK KYRKEQRKLR QAVKDAVSKK PIPLENPKEK RPGKRIEREE EEEEEALPL DMMDEDDLQL MKDLGQRVSF LTRDLSSSEP VHAKKRKHER IIDKYEKIPR TLQTAPEKEL IHLLPIKDKS G IIPQTREK PVTDSNKDEE DQEEERELEE EIIEDPIQEL TIEEHLIERK KKLQEKKMHI AALASAILSD PENNIKKLKE LR SMLMEQD PDVAVTVRKL VIVSLMELFK DITPSYKIRP LTEAEKSTKT RKETQKLREF EEGLVSQYKF YLENLEQMVK DWK QRKLKK SNVVSLKAYK GLAEVAVKSL CELLVALPHF NFHNNIIVLI VPLMNDMSKL ISEMCCEAVK KLFKQDKLGQ ASLG VIKVI SGFVKGRNYE VRPEMLKTFL CLRIKEVEVK KDTEDINKPK KFMTFKEKRK SLSRMQRKWK KAEEKLEREL REAEA SEST EKKLKLHTET LNIVFVTYFR ILKKAQRSPL LPAVLEGLAK FAHLINVEFF DDLLVVLHTL IESGDLSYQE SLHCVQ TAF HILSGQGDVL NIDPLKFYTH LYKTLFKLHA GATNEGVEIV LQCLDVMLTK RRKQVSQQRA LAFIKRLCTL ALHVLPN SS IGILATTRIL MHTFPKTDLL LDSESQGSGV FLPELDEPEY CNAQNTALWE LHALRRHYHP IVQRFAAHLI AGAPSEGS G ALKPELSRRS ATELFEAYSM AEMTFNPPVE SSNPKIKGKF LQGDSFLNED LNQLIKRYSS EVATESPLDF TKYLKTSLH

UniProtKB: Nucleolar complex protein 3 homolog

分子 #50: Probable ribosome biogenesis protein RLP24

• 分子: 名称: Probable ribosome biogenesis protein RLP24 / タイプ: protein_or_peptide / ID: 50 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 19.666258 KDa

配列

文字列:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

分子 #51: ATP-dependent RNA helicase DDX18

• 分子: 名称: ATP-dependent RNA helicase DDX18 / タイプ: protein_or_peptide / ID: 51 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 75.526789 KDa

配列

文字列:

MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQK STVLTNGEAA MQSSNSESKK KKKKKRKMVN DAEPDTKKAK TENKGKSEEE SAETTKETEN NVEKPDNDED E SEVPSLPL GLTGAFEDTS FASLCNLVNE NTLKAIKEMG FTNMTEIQHK SIRPLLEGRD LLAAAKTGSG KTLAFLIPAV EL IVKLRFM PRNGTGVLIL SPTRELAMQT FGVLKELMTH HVHTYGLIMG GSNRSAEAQK LGNGINIIVA TPGRLLDHMQ NTP GFMYKN LQCLVIDEAD RILDVGFEEE LKQIIKLLPT RRQTMLFSAT QTRKVEDLAR ISLKKEPLYV GVDDDKANAT VDGL EQGYV VCPSEKRFLL LFTFLKKNRK KKLMVFFSSC MSVKYHYELL NYIDLPVLAI HGKQKQNKRT TTFFQFCNAD SGTLL CTDV AARGLDIPEV DWIVQYDPPD DPKEYIHRVG RTARGLNGRG HALLILRPEE LGFLRYLKQS KVPLSEFDFS WSKISD IQS QLEKLIEKNY FLHKSAQEAY KSYIRAYDSH SLKQIFNVNN LNLPQVALSF GFKVPPFVDL NVNSNEGKQK KRGGGGG FG YQKTKKVEKS KIFKHISKKS SDSRQFSH

UniProtKB: ATP-dependent RNA helicase DDX18

分子 #52: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase

• 分子: 名称: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; タイプ: protein_or_peptide / ID: 52 / コピー数: 1 / 光学異性体: LEVO; EC番号: 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 89.441836 KDa

配列

文字列:

MGRKLDPTKE KRGPGRKARK QKGAETELVR FLPAVSDENS KRLSSRARKR AAKRRLGSVE APKTNKSPEA KPLPGKLPKG ISAGAVQTA GKKGPQSLFN APRGKKRPAP GSDEEEEEED SEEDGMVNHG DLWGSEDDAD TVDDYGADSN SEDEEEGEAL L PIERAARK QKAREAAAGI QWSEEETEDE EEEKEVTPES GPPKVEEADG GLQINVDEEP FVLPPAGEME QDAQAPDLQR VH KRIQDIV GILRDFGAQR EEGRSRSEYL NRLKKDLAIY YSYGDFLLGK LMDLFPLSEL VEFLEANEVP RPVTLRTNTL KTR RRDLAQ ALINRGVNLD PLGKWSKTGL VVYDSSVPIG ATPEYLAGHY MLQGASSMLP VMALAPQEHE RILDMCCAPG GKTS YMAQL MKNTGVILAN DANAERLKSV VGNLHRLGVT NTIISHYDGR QFPKVVGGFD RVLLDAPCSG TGVISKDPAV KTNKD EKDI LRCAHLQKEL LLSAIDSVNA TSKTGGYLVY CTCSITVEEN EWVVDYALKK RNVRLVPTGL DFGQEGFTRF RERRFH PSL RSTRRFYPHT HNMDGFFIAK FKKFSNSIPQ SQTGNSETAT PTNVDLPQVI PKSENSSQPA KKAKGAAKTK QQLQKQQ HP KKASFQKLNG ISKGADSELS TVPSVTKTQA SSSFQDSSQP AGKAEGIREP KVTGKLKQRS PKLQSSKKVA FLRQNAPP K GTDTQTPAVL SPSKTQATLK PKDHHQPLGR AKGVEKQQLP EQPFEKAAFQ KQNDTPKGPQ PPTVSPIRSS RPPPAKRKK SQSRGNSQLL LS

UniProtKB: 28S rRNA (cytosine(4447)-C(5))-methyltransferase

分子 #53: Nucleolar protein 16

• 分子: 名称: Nucleolar protein 16 / タイプ: protein_or_peptide / ID: 53 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.234486 KDa

配列

文字列:

MPKAKGKTRR QKFGYSVNRK RLNRNARRKA APRIECSHIR HAWDHAKSVR QNLAEMGLAV DPNRAVPLRK RKVKAMEVDI EERPKELVR KPYVLNDLEA EASLPEKKGN TLSRDLIDYV RYMVENHGED YKAMARDEKN YYQDTPKQIR SKINVYKRFY P AEWQDFLD SLQKRKMEVE

UniProtKB: Nucleolar protein 16

分子 #3: 5.8S rRNA

• 分子: 名称: 5.8S rRNA / タイプ: rna / ID: 3 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 50.449812 KDa

配列

文字列:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

GENBANK: GENBANK: U13369.1

分子 #4: ITS2 rRNA

• 分子: 名称: ITS2 rRNA / タイプ: rna / ID: 4 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 376.425312 KDa

配列

文字列:

GCCGAUCAAU CGCCCCCGGG GGUGCCUCCG GGCUCCUCGG GGUGCGCGGC UGGGGGUUCC CUCGCAGGGC CCGCCGGGGG CCCUCCGUC CCCCUAAGCG CAGACCCGGC GGCGUCCGCC CUCCUCUUGC CGCCGCGCCC GCCCCUUCCC CCUCCCCCCG C GGGCCCUG CGUGGUCACG CGUCGGGUGG CGGGGGGGAG AGGGGGGCGC GCCCGGCUGA GAGAGACGGG GAGGGCGGCG CC GCCGCCG CCCGCGAAGA CGGAGAGGGA AAGAGAGAGC CGGCUCGGGC CGAGUUCCCG UGGCCGCCGC CUGCGGUCCG GGU UCCUCC CUCGGGGGGC UCCCUCGCGC CGCGCGCGGC UCGGGGUUCG GGGUUCGUCG GCCCCGGCCG GGUGGAAGGU CCCG UGCCC GUCGUCGUCG UCGUCGUCGC GCGUCGUCGG CGGUGGGGGC GUGUUGCGUG CGGUGUGGUG GUGGGGGAGG AGGAA GGCG GGUCCGGAAG GGGAAGGGUG CCGGCGGGGA GAGAGGGUCG GGGGAGCGCG UCCCGGUCGC CGCGGUUCGC CGCCCG CCC CCGGUGGCGG CCCGGCGUCC GGCCGACCGC CGCUCCCGCG CCCCUCCUCC UCCCCGCCGC CCCUCCUCCG AGGCCCC GC CCGUCCUCCU CGCCCUCCCC GCGCGUACGC GCGCCCGCCC GCCCGGCUCG CCUCGCGGCG CGUCGGCCGG GGCCGGGA G CCCGCCCCGC GGCCCGCCCG GCCGCGCCCG UGGCCGCGGC GCCGGGGUUC GCGUGUCCCC GGCGGCGACC CGCGGGACG CCGCGGUGUC GUCCGCCGUC GCGCGCCCGC CUCCGGCUCG CGGCCGCGCC GCGCCGCGCC GGGGCCCCGU CCCGAGCUUC CGCGUCGGG GCGGGGCGGC UCCGCCGCCG CGUCCUCGGA CCCGUCCCCC CGACCUCCGC GGGGGAGACG GGUCGGGGCG U GCGGCGCC CGUCCCGCCC CCGGCCCGUG CCCCUCCCUC CGGUCGUCCC GCUCCGGCGG GGCGGCGCGG GGGUGCCGCC GG CCGCGCG CUCUCUCUCC CGUCGCCUCU CCCCCUCGCC GGGCCCGUCU CCCGACGGAG CGUCGGGCGG GCGGUCGGGC CGG CGCGAU UCCGUCCGUC CGUCCGCCGA GCGGCCCGUC CCCCUCCGAG A

GENBANK: GENBANK: AL592188.60

分子 #5: 28S rRNA

• 分子: 名称: 28S rRNA / タイプ: rna / ID: 5 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 1.640222125 MDa

配列

文字列:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCCCUCCC UUCCCC CGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCGGGGG UGGGGUC GG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCG G GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCUCC UCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGGG A GCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCCG CG GGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CCCUCCUCCC CGC GCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGGG UCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGGAGCGAG CGCAC GGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC CGUCUUGAAA CACGGACCAA GGAGUCUAAC ACGUGCGCGA GUCGGG GGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGGU GGGAUCCCGA GGCCUCU CC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACC C GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGG CGCUCUCGCA GACCCGACGC ACCCCCGCCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG G GCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGGAGCC GGGCGUGGAA UG CGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGC CGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAG GAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGU CGCC GGCAGUCGAG AGUGGACGGG AGCGGCGGGG GCGGCGCGCG CGCGCGCGCG UGUGGUGUGC GUCGGAGGGC GGCGGC GGC GGCGGCGGCG GGGGUGUGGG GUCCUUCCCC CGCCCCCCCC CCCACGCCUC CUCCCCUCCU CCCGCCCACG CCCCGCU CC CCGCCCCCGG AGCCCCGCGG ACGCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAGC CUUGAAGCCU AGGGCGCG G GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU UCAAACGAGA ACUUUGAAGG CCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCCCCGAAUC CGGAGUGGCG GAGAUGGGCG C CGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UG AAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCC GGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGCGCCG GGCCGUACCC AUAUCCGCAG CAGG UCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGUCGG CAAGCCGGAU CCGUAACUUC GGGAU AAGG AUUGGCUCUA AGGGCUGGGU CGGUCGGGCU GGGGCGCGAA GCGGGGCUGG GCGCGCGCCG CGGCUGGACG AGGCGC CGC CGCCCCCCCC ACGCCCGGGG CACCCCCCUC GCGGCCCUCC CCCGCCCCAC CCCGCGCGCG CCGCUCGCUC CCUCCCC GC CCCGCGCCCU CUCUCUCUCU CUCUCCCCCG CUCCCCGUCC UCCCCCCUCC CCGGGGGAGC GCCGCGUGGG GGCGGCGG C GGGGGGAGAA GGGUCGGGGC GGCAGGGGCC GGCGGCGGCC CGCCGCGGGG CCCCGGCGGC GGGGGCACGG UCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGG GCGUCGCGGC CGCCCCCGGG GAGCCCGGCG GGCGCCGGCG CGCCCCCCCC CCCACCCCAC GUCUCGUCGC G CGCGCGUC CGCUGGGGGC GGGGAGCGGU CGGGCGGCGG CGGUCGGCGG GCGGCGGGGC GGGGCGGUUC GUCCCCCCGC CC UACCCCC CCGGCCCCGU CCGCCCCCCG UUCCCCCCUC CUCCUCGGCG CGCGGCGGCG GCGGCGGCAG GCGGCGGAGG GGC CGCGGG CCGGUCCCCC CCGCCGGGUC CGCCCCCGGG GCCGCGGUUC CGCGCGGCGC CUCGCCUCGG CCGGCGCCUA GCAG CCGAC UUAGAACUGG UGCGGACCAG GGGAAUCCGA CUGUUUAAUU AAAACAAAGC AUCGCGAAGG CCCGCGGCGG GUGUU GACG CGAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAU GAAGCGCGGG UAAACGGCGG GAGUAA CUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGU CC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCU U GACUCUAGUC UGGCACGGUG AAGAGACAUG AGAGGUGUAG AAUAAGUGGG AGGCCCCCGG CGCCCCCCCG GUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCG GGGGGGCGAG CCCCGAGGGG CUCUCGCUUC UGGCGCCAAG CGCCCGGCCG CGCGCCGGCC GGGCGCGACC C GCUCCGGG GACAGUGCCA GGUGGGGAGU UUGACUGGGG CGGUACACCU GUCAAACGGU AACGCAGGUG UCCUAAGGCG AG CUCAGGG AGGACAGAAA CCUCCCGUGG AGCAGAAGGG CAAAAGCUCG CUUGAUCUUG AUUUUCAGUA CGAAUACAGA CCG UGAAAG CGGGGCCUCA CGAUCCUUCU GACCUUUUGG GUUUUAAGCA GGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUG GCUUG UGGCGGCCAA GCGUUCAUAG CGACGUCGCU UUUUGAUCCU UCGAUGUCGG CUCUUCCUAU CAUUGUGAAG CAGAA UUCA CCAAGCGUUG GAUUGUUCAC CCACUAAUAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUAC CCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGC UU GGCUGAGGAG CCAAUGGGGC GAAGCUACCA UCUGUGGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCC GCCCAGGC G GAACGAUACG GCAGCGCCGC GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCUGU CCCCGCCGGC GGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUG GGAAACGGGG CGCGGCCGGA GAGGCGGCCG CCCCCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU G GCGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUCCCUCG CUGCGAUCUA UU GAAAGUC AGCCCUCGAC ACAAGGGUUU GUC

GENBANK: GENBANK: AL592188.60

分子 #54: MAGNESIUM ION

• 分子: 名称: MAGNESIUM ION / タイプ: ligand / ID: 54 / コピー数: 61 / 式: MG
• 分子量: 理論値: 24.305 Da

分子 #55: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 55 / コピー数: 2 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #56: ADENOSINE-5'-DIPHOSPHATE

• 分子: 名称: ADENOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 56 / コピー数: 1 / 式: ADP
• 分子量: 理論値: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM

分子 #57: GUANOSINE-5'-DIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 57 / コピー数: 1 / 式: GDP
• 分子量: 理論値: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM

分子 #58: POTASSIUM ION

• 分子: 名称: POTASSIUM ION / タイプ: ligand / ID: 58 / コピー数: 1 / 式: K
• 分子量: 理論値: 39.098 Da

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 緩衝液: pH: 7.6
• グリッド: モデル: Quantifoil R3.5/1 / 材質: GOLD / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 2 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec.
• 凍結: 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 283 K / 装置: FEI VITROBOT MARK IV; 詳細: Four applications with manual blotting before last blotting with the vitrobot..

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 特殊光学系: エネルギーフィルター - スリット幅: 20 eV
• 撮影: フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 撮影したグリッド数: 4 / 実像数: 172699 / 平均露光時間: 2.0 sec. / 平均電子線量: 60.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2.5 µm / 最小 デフォーカス（公称値）: 0.5 µm / 倍率（公称値）: 64000
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

• 粒子像選択: 選択した数: 15679142
• 初期モデル: モデルのタイプ: INSILICO MODEL
• 最終 再構成: 想定した対称性 - 点群: C₁ (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 2.59 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: cryoSPARC (ver. 3.3.2) / 使用した粒子像数: 126301
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 4.0)
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC (ver. 3.3.2)
• 最終 3次元分類: ソフトウェア: (名称: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

原子モデル構築 1

• 精密化: 空間: REAL

得られたモデル

PDB-8fkx:
Human nucleolar pre-60S ribosomal subunit (State E)