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- EMDB-2884: Cryo-Molecular electron tomography of PfEMP1-IT4Var60) -

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Basic information

Entry
Database: EMDB / ID: EMD-2884
TitleCryo-Molecular electron tomography of PfEMP1-IT4Var60)
Map dataReconstruction of PfEMP1-IT4Var60 ectodomain
Sample
  • Sample: ectodomain of PfEMP1-IT4Var60
  • Protein or peptide: PfEMP1
KeywordsMalaria / Rosetting / PfEMP1
Biological speciesPlasmodium falciparum (malaria parasite P. falciparum)
Methodelectron tomography / cryo EM / Resolution: 20.0 Å
AuthorsAkhouri RR / Goel S / Furusho H / Skoglund U / Wahlgren M
CitationJournal: Cell Rep / Year: 2016
Title: Architecture of Human IgM in Complex with P. falciparum Erythrocyte Membrane Protein 1.
Authors: Reetesh Raj Akhouri / Suchi Goel / Hirotoshi Furusho / Ulf Skoglund / Mats Wahlgren /
Abstract: Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex with non-immune immunoglobulin M (IgM) is common among ...Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex with non-immune immunoglobulin M (IgM) is common among parasites that cause both severe childhood malaria and pregnancy-associated malaria. Here, we present cryo-molecular electron tomography structures of human IgM, PfEMP1 and their complex. Three-dimensional reconstructions of IgM reveal that it has a dome-like core, randomly oriented Fab2s units, and the overall shape of a turtle. PfEMP1 is a C- shaped molecule with a flexible N terminus followed by an arc-shaped backbone and a bulky C terminus that interacts with IgM. Our data demonstrate that the PfEMP1 binding pockets on IgM overlap with those of C1q, and the bulkiness of PfEMP1 limits the capacity of IgM to interact with PfEMP1. We suggest that P. falciparum exploits IgM to cluster PfEMP1 into an organized matrix to augment its affinity to host cell receptors.
History
DepositionFeb 8, 2015-
Header (metadata) releaseApr 1, 2015-
Map releaseJan 13, 2016-
UpdateFeb 17, 2016-
Current statusFeb 17, 2016Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.013
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.013
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_2884.tif

Downloads & links

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Map

FileDownload / File: emd_2884.map.gz / Format: CCP4 / Size: 1.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of PfEMP1-IT4Var60 ectodomain
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.27 Å/pix.
x 77 pix.
= 174.559 Å		2.27 Å/pix.
x 103 pix.
= 233.501 Å		2.27 Å/pix.
x 62 pix.
= 140.554 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 2.267 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.013 / Movie #1: 0.013
Minimum - Maximum0.00021451 - 0.02836445
Average (Standard dev.)0.00636834 (±0.00430598)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions1036277
Spacing1036277
CellA: 140.554 Å / B: 233.50099 Å / C: 174.55899 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.2672.2672.267
M x/y/z6210377
origin x/y/z0.0000.0000.000
length x/y/z140.554233.501174.559
α/β/γ90.00090.00090.000
start NX/NY/NZ-180-180-179
NX/NY/NZ360360360
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS6210377
D min/max/mean0.0000.0280.006

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Supplemental data

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Sample components

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Entire : ectodomain of PfEMP1-IT4Var60

EntireName: ectodomain of PfEMP1-IT4Var60
Components
  • Sample: ectodomain of PfEMP1-IT4Var60
  • Protein or peptide: PfEMP1

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Supramolecule #1000: ectodomain of PfEMP1-IT4Var60

SupramoleculeName: ectodomain of PfEMP1-IT4Var60 / type: sample / ID: 1000 / Details: the sample was monodisperse. / Oligomeric state: Monomer / Number unique components: 1
Molecular weightExperimental: 358 KDa / Theoretical: 280 KDa

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Macromolecule #1: PfEMP1

MacromoleculeName: PfEMP1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Oligomeric state: monomer / Recombinant expression: Yes
Source (natural)Organism: Plasmodium falciparum (malaria parasite P. falciparum)
Strain: FCR3S1.2 / synonym: Malaria Parasite / Location in cell: Infected erythrocyte membrane
Molecular weightExperimental: 358 KDa / Theoretical: 280 KDa
Recombinant expressionOrganism: Drosophila melanogaster (fruit fly) / Recombinant cell: S2 cells / Recombinant plasmid: pMTBipV5HisA

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation stateparticle

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Sample preparation

Concentration2 mg/mL
BufferpH: 7.5 / Details: 20mM Hepes, 200mM NaCl
GridDetails: C-Flat (Copper grid with thin Carbon support)
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 78 K / Instrument: FEI VITROBOT MARK IV / Method: Blot for 4 seconds before plunging

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Electron microscopy

MicroscopeFEI TITAN KRIOS
TemperatureMin: 78 K / Max: 100 K
Specialist opticsEnergy filter - Name: FEI
DateSep 13, 2013
Image recordingCategory: CCD / Film or detector model: FEI FALCON II (4k x 4k) / Number real images: 281 / Average electron dose: 40 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 0.0015 µm / Nominal defocus min: 0.0007 µm / Nominal magnification: 37000
Sample stageSpecimen holder: LN2 cooled / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Tilt series - Axis1 - Min angle: -70 ° / Tilt series - Axis1 - Max angle: 70 ° / Tilt series - Axis1 - Angle increment: 0.5 °
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsCTF correction and alignment using gold particles
Final reconstructionResolution.type: BY AUTHOR / Resolution: 20.0 Å / Software - Name: COMET / Number images used: 281
CTF correctionDetails: each frame

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