ジャーナル: Elife / 年: 2015 タイトル: Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1. 著者: Agata Butryn / Jan M Schuller / Gabriele Stoehr / Petra Runge-Wollmann / Friedrich Förster / David T Auble / Karl-Peter Hopfner / 要旨: Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent ...Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box-binding protein (TBP):DNA complexes, offering a useful system to address the structural mechanisms of Swi2/Snf2 ATPases. Here, we report the crystal structure of the N-terminal domain of Mot1 in complex with TBP, DNA, and the transcription regulator negative cofactor 2 (NC2). Our data show that Mot1 reduces DNA:NC2 interactions and unbends DNA as compared to the TBP:DNA:NC2 state, suggesting that Mot1 primes TBP:NC2 displacement in an ATP-independent manner. Electron microscopy and cross-linking data suggest that the Swi2/Snf2 domain of Mot1 associates with the upstream DNA and the histone fold of NC2, thereby revealing parallels to some nucleosome remodelers. This study provides a structural framework for how a Swi2/Snf2 ATPase interacts with its substrate DNA:protein complex.
ダウンロード / ファイル: emd_2828.map.gz / 形式: CCP4 / 大きさ: 12.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Negative Stain reconstruction of the Mot1 TBP NC2 complex
ボクセルのサイズ
X=Y=Z: 1.61 Å
密度
表面レベル
登録者による: 0.03 / ムービー #1: 0.03
最小 - 最大
-0.04712194 - 0.15408267
平均 (標準偏差)
0.00092478 (±0.01138391)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
150
150
150
Spacing
150
150
150
セル
A=B=C: 241.5 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.61
1.61
1.61
M x/y/z
150
150
150
origin x/y/z
0.000
0.000
0.000
length x/y/z
241.500
241.500
241.500
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
150
150
150
D min/max/mean
-0.047
0.154
0.001
-
添付データ
-
試料の構成要素
-
全体 : Mot1 in complex with TBP, NC2a, NC2b and DNA
全体
名称: Mot1 in complex with TBP, NC2a, NC2b and DNA
要素
試料: Mot1 in complex with TBP, NC2a, NC2b and DNA
タンパク質・ペプチド: Mot1
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超分子 #1000: Mot1 in complex with TBP, NC2a, NC2b and DNA
超分子
名称: Mot1 in complex with TBP, NC2a, NC2b and DNA / タイプ: sample / ID: 1000 詳細: only the C-terminal part of Mot1 was used in the construct Number unique components: 5