|Entry||Database: EMDB / ID: 2739|
|Title||Conformational Snapshots of Inducible Nitric Oxide Synthase (iNOS)|
|Map data||Single-particle reconstruction of iNOS: Group II, Conformation vii|
|Sample||Murine Inducible Nitric Oxide Synthase|
|Keywords||heterogeneity / random conical tilt / nitric oxide synthase / calmodulin / heme / electron transfer / flavin|
|Function / homology||FAD-binding, type 1 / Flavoprotein-like superfamily / NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily / Nitric oxide synthase, N-terminal / Oxidoreductase NAD-binding domain / Riboflavin synthase-like beta-barrel / Flavodoxin / Ferredoxin reductase-type FAD-binding domain / FAD binding domain / Nitric-oxide synthase, eukaryote ...FAD-binding, type 1 / Flavoprotein-like superfamily / NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily / Nitric oxide synthase, N-terminal / Oxidoreductase NAD-binding domain / Riboflavin synthase-like beta-barrel / Flavodoxin / Ferredoxin reductase-type FAD-binding domain / FAD binding domain / Nitric-oxide synthase, eukaryote / Flavodoxin/nitric oxide synthase / Nitric oxide synthase, oxygenase domain / Nitric oxide synthase, N-terminal domain superfamily / Nitric oxide synthase (NOS) signature. / Flavoprotein pyridine nucleotide cytochrome reductase / Oxidoreductase FAD/NAD(P)-binding / Peroxisomal protein import / Nitric oxide stimulates guanylate cyclase / ROS, RNS production in phagocytes / Ferredoxin reductase-type FAD binding domain profile. / Flavodoxin-like / Flavodoxin-like domain profile. / interleukin-6 secretion / prostaglandin secretion / Ser/Thr protein kinase, TGFB receptor / positive regulation of killing of cells of other organism / tetrahydrobiopterin binding / interleukin-8 secretion / cortical cytoskeleton / arginine binding / superoxide metabolic process / regulation of cytokine production involved in inflammatory response / peptidyl-cysteine S-nitrosylation / cellular response to cytokine stimulus / regulation of insulin secretion / cellular response to organic cyclic compound / nitric-oxide synthase / positive regulation of guanylate cyclase activity / cellular response to interferon-gamma / nitric oxide mediated signal transduction / negative regulation of blood pressure / arginine catabolic process / nitric-oxide synthase activity / NADPH-hemoprotein reductase activity / nitric oxide biosynthetic process / negative regulation of protein catabolic process / cellular response to drug / peroxisome / positive regulation of blood vessel diameter / circadian rhythm / FMN binding / NADP binding / flavin adenine dinucleotide binding / response to lipopolysaccharide / regulation of cell proliferation / calmodulin binding / negative regulation of gene expression / cellular response to lipopolysaccharide / response to hypoxia / inflammatory response / iron ion binding / intracellular / defense response to bacterium / signaling receptor binding / heme binding / perinuclear region of cytoplasm / protein homodimerization activity / metal ion binding / cytosol / cytoplasm / Nitric oxide synthase, inducible|
Function and homology information
|Source||Mus musculus (house mouse)|
|Method||single particle reconstruction / negative staining / 67 Å resolution|
|Authors||Campbell MG / Smith BC / Potter CS / Carragher B / Marletta MA|
|Citation||Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2014|
Title: Molecular architecture of mammalian nitric oxide synthases.
Authors: Melody G Campbell / Brian C Smith / Clinton S Potter / Bridget Carragher / Michael A Marletta
|Date||Deposition: Jul 29, 2014 / Header (metadata) release: Sep 3, 2014 / Map release: Sep 10, 2014 / Last update: Sep 17, 2014|
|Structure viewer||EM map: |
Downloads & links
|File||emd_2739.map.gz (map file in CCP4 format, 170369 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.36 Å|
CCP4 map header:
-Entire Murine Inducible Nitric Oxide Synthase
|Entire||Name: Murine Inducible Nitric Oxide Synthase / Details: Sample is highly flexible / Number of components: 1 / Oligomeric State: Homodimer|
|Mass||Theoretical: 260 kDa|
-Component #1: protein, Inducible Nitric Oxide Synthase
|Protein||Name: Inducible Nitric Oxide SynthaseNitric oxide synthase / a.k.a: iNOSNitric oxide synthase / Oligomeric Details: Homodimer / Recombinant expression: Yes / Number of Copies: 1|
|Mass||Theoretical: 260 kDa|
|Source||Species: Mus musculus (house mouse)|
|Source (engineered)||Expression System: Escherichia coli (E. coli) / Vector: pCWiNOS / Cell of expression system: JM109|
|Source (natural)||Cell: Macrophages|
|External references||InterPro: Ser/Thr protein kinase, TGFB receptor / UniProt: Nitric oxide synthase, inducible|
Gene Ontology: nitric oxide biosynthetic process, FMN binding, NADP binding, calmodulin binding, flavin adenine dinucleotide binding, heme binding, iron ion binding, nitric-oxide synthase activity
|Specimen||Specimen state: particle / Method: negative staining|
|Sample solution||Buffer solution: 50 mM TEA pH 7.5, 150 mM NaCl, and 5 mM DTT|
|Support film||Glow discharged C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbon|
|Staining||3 microliters of sample were applied to grid. The specimen was stained twice with 2% uranyl formate, then allowed to air-dry.|
|Vitrification||Instrument: NONE / Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai F20 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F20 / Date: Mar 6, 2013|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 37 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 62000 X (nominal), 114705 X (calibrated) / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000 - 2500 nm|
|Specimen Holder||Model: SIDE ENTRY, EUCENTRIC / Tilt Angle: 0 - 55 deg.|
|Camera||Detector: TVIPS TEMCAM-F416 (4k x 4k)|
Eachareais imagedtwic eonceat55d egreesanda gainwithno tilt
|Processing||Method: single particle reconstruction / Number of class averages: 1 / Applied symmetry: C1 (asymmetric) / Number of projections: 207 / Details: See publication.|
|3D reconstruction||Algorithm: RCT / Software: Appion, Spider / CTF correction: Each Image / Details: See publication. / Resolution: 67 Å / Resolution method: FSC 0.5, semi-independent|
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