|Entry||Database: EMDB / ID: 2739|
|Title||Conformational Snapshots of Inducible Nitric Oxide Synthase (iNOS)|
|Keywords||heterogeneity / random conical tilt / nitric oxide synthase / calmodulin / heme / electron transfer / flavin|
|Sample||Murine Inducible Nitric Oxide Synthase|
|Source||Mus musculus / mammal / Mouse / ハツカネズミ, はつかねずみ /|
|Map data||Single-particle reconstruction of iNOS: Group II, Conformation vii|
|Method||single particle reconstruction, at 67 Å resolution|
|Authors||Campbell MG / Smith BC / Potter CS / Carragher B / Marletta MA|
|Citation||Proc. Natl. Acad. Sci. U.S.A., 2014, 111, E3614-E3623|
Proc. Natl. Acad. Sci. U.S.A., 2014, 111, E3614-E3623 Yorodumi Papers
|Date||Deposition: Jul 29, 2014 / Header (metadata) release: Sep 3, 2014 / Map release: Sep 10, 2014 / Last update: Sep 17, 2014|
Downloads & links
|File||emd_2739.map.gz (map file in CCP4 format, 170369 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 1.36 Å|
CCP4 map header:
-Entire Murine Inducible Nitric Oxide Synthase
|Entire||Name: Murine Inducible Nitric Oxide Synthase / Details: Sample is highly flexible / Number of components: 1 / Oligomeric State: Homodimer|
|Mass||Theoretical: 260 kDa|
-Component #1: protein, Inducible Nitric Oxide Synthase
|Protein||Name: Inducible Nitric Oxide Synthase / a.k.a: iNOS / Oligomeric Details: Homodimer / Recombinant expression: Yes / Number of Copies: 1|
|Mass||Theoretical: 260 kDa|
|Source||Species: Mus musculus / mammal / Mouse / ハツカネズミ, はつかねずみ /|
|Source (engineered)||Expression System: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 / |
Vector: pCWiNOS / Cell of expression system: JM109
|Source (natural)||Cell: Macrophages|
|External references||InterPro: InterPro: 000333 / UniProt: UniProt: P29477|
Gene Ontology: GO: 0006809, GO: 0010181, GO: 0050661, GO: 0005516, GO: 0050660, GO: 0020037, GO: 0005506, GO: 0004517
|Sample solution||Buffer solution: 50 mM TEA pH 7.5, 150 mM NaCl, and 5 mM DTT|
|Support film||Glow discharged C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbon|
|Staining||3 microliters of sample were applied to grid. The specimen was stained twice with 2% uranyl formate, then allowed to air-dry.|
|Vitrification||Instrument: NONE / Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai F20 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F20 / Date: Mar 6, 2013|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 37 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 62000 X (nominal), 114705 X (calibrated) / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000 - 2500 nm|
|Specimen Holder||Model: SIDE ENTRY, EUCENTRIC / Tilt Angle: 0 - 55 deg.|
|Camera||Detector: TVIPS TEMCAM-F416 (4k x 4k)|
Eachareais imagedtwic eonceat55d egreesanda gainwithno tilt
|Processing||Method: single particle reconstruction / Number of class averages: 1 / Applied symmetry: C1 (asymmetric) / Number of projections: 207 / Details: See publication.|
|3D reconstruction||Algorithm: RCT / Software: Appion, Spider / CTF correction: Each Image / Details: See publication. / Resolution: 67 Å / Resolution method: FSC 0.5, semi-independent|
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