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- EMDB-2739: Conformational Snapshots of Inducible Nitric Oxide Synthase (iNOS) -

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Basic information

Database: EMDB / ID: 2739
TitleConformational Snapshots of Inducible Nitric Oxide Synthase (iNOS)
Keywordsheterogeneity / random conical tilt / nitric oxide synthase / calmodulin / heme / electron transfer / flavin
SampleMurine Inducible Nitric Oxide Synthase
SourceMus musculus / mammal / Mouse / ハツカネズミ, はつかねずみ /
Map dataSingle-particle reconstruction of iNOS: Group II, Conformation vii
Methodsingle particle reconstruction, at 67 Å resolution
AuthorsCampbell MG / Smith BC / Potter CS / Carragher B / Marletta MA
CitationProc. Natl. Acad. Sci. U.S.A., 2014, 111, E3614-E3623

Proc. Natl. Acad. Sci. U.S.A., 2014, 111, E3614-E3623 Yorodumi Papers
Molecular architecture of mammalian nitric oxide synthases.
Melody G Campbell / Brian C Smith / Clinton S Potter / Bridget Carragher / Michael A Marletta

DateDeposition: Jul 29, 2014 / Header (metadata) release: Sep 3, 2014 / Map release: Sep 10, 2014 / Last update: Sep 17, 2014

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.882
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by height
  • Surface level: 0.882
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

fix: /
Orientation Rotation
Misc. /
Supplemental images

Downloads & links


Fileemd_2739.map.gz (map file in CCP4 format, 170369 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
352 pix
1.36 Å/pix.
= 478.72 Å
352 pix
1.36 Å/pix.
= 478.72 Å
352 pix
1.36 Å/pix.
= 478.72 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.36 Å
Contour Level:0.882 (by author), 0.882 (movie #1):
Minimum - Maximum-0.84902978 - 2.34935594
Average (Standard dev.)-0.00679095 (0.11729628)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 478.72 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.361.361.36
M x/y/z352352352
origin x/y/z0.0000.0000.000
length x/y/z478.720478.720478.720
start NX/NY/NZ000
MAP C/R/S123
start NC/NR/NS-176-176-176
D min/max/mean-0.8492.349-0.007

Supplemental data

Sample components

Entire Murine Inducible Nitric Oxide Synthase

EntireName: Murine Inducible Nitric Oxide Synthase / Details: Sample is highly flexible / Number of components: 1 / Oligomeric State: Homodimer
MassTheoretical: 260 kDa

Component #1: protein, Inducible Nitric Oxide Synthase

ProteinName: Inducible Nitric Oxide Synthase / a.k.a: iNOS / Oligomeric Details: Homodimer / Recombinant expression: Yes / Number of Copies: 1
MassTheoretical: 260 kDa
SourceSpecies: Mus musculus / mammal / Mouse / ハツカネズミ, はつかねずみ /
Source (engineered)Expression System: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
Vector: pCWiNOS / Cell of expression system: JM109
Source (natural)Cell: Macrophages
External referencesInterPro: InterPro: 000333 / UniProt: UniProt: P29477
Gene Ontology: GO: 0006809, GO: 0010181, GO: 0050661, GO: 0005516, GO: 0050660, GO: 0020037, GO: 0005506, GO: 0004517

Experimental details

Sample preparation

Specimen stateparticle
Sample solutionBuffer solution: 50 mM TEA pH 7.5, 150 mM NaCl, and 5 mM DTT
pH: 7.5
Support filmGlow discharged C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbon
Staining3 microliters of sample were applied to grid. The specimen was stained twice with 2% uranyl formate, then allowed to air-dry.
VitrificationInstrument: NONE / Cryogen name: NONE

Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20 / Date: Mar 6, 2013
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 37 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 62000 X (nominal), 114705 X (calibrated) / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000 - 2500 nm
Specimen HolderModel: SIDE ENTRY, EUCENTRIC / Tilt Angle: 0 - 55 deg.
CameraDetector: TVIPS TEMCAM-F416 (4k x 4k)

Image acquisition

Image acquisition
Image acquisition
Eachareais imagedtwic eonceat55d egreesanda gainwithno tilt

Image processing

ProcessingMethod: single particle reconstruction / Number of class averages: 1 / Applied symmetry: C1 (asymmetric) / Number of projections: 207 / Details: See publication.
3D reconstructionAlgorithm: RCT / Software: Appion, Spider / CTF correction: Each Image / Details: See publication. / Resolution: 67 Å / Resolution method: FSC 0.5, semi-independent

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