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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Human mitochondrial small subunit assembly intermediate (State B) | |||||||||
![]() | Mitochondrial small subunit assembly intermediate (State A) | |||||||||
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![]() | Ribonucleoprotein complex / Mitochondria Biogenesis / RIBOSOME | |||||||||
機能・相同性 | ![]() mitochondrial small ribosomal subunit assembly / rRNA modification in the mitochondrion / mitochondrial transcription factor activity / transcription initiation at mitochondrial promoter / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / Mitochondrial Fatty Acid Beta-Oxidation / mitochondrial ribosome binding / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity ...mitochondrial small ribosomal subunit assembly / rRNA modification in the mitochondrion / mitochondrial transcription factor activity / transcription initiation at mitochondrial promoter / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / Mitochondrial Fatty Acid Beta-Oxidation / mitochondrial ribosome binding / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation termination / positive regulation of mitochondrial translation / Mitochondrial translation initiation / S-adenosyl-L-methionine binding / negative regulation of mitotic nuclear division / mitochondrial small ribosomal subunit / rRNA methylation / mitochondrial ribosome / mitochondrial translation / fatty acid beta-oxidation / mitochondrial nucleoid / positive regulation of proteolysis / ribosomal small subunit binding / Mitochondrial protein degradation / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / apoptotic signaling pathway / Transcriptional activation of mitochondrial biogenesis / fatty acid biosynthetic process / cell junction / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / 4 iron, 4 sulfur cluster binding / small ribosomal subunit / small ribosomal subunit rRNA binding / nuclear membrane / cell population proliferation / mitochondrial inner membrane / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / translation / protein domain specific binding / intracellular membrane-bounded organelle / mRNA binding / nucleolus / GTP binding / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.54 Å | |||||||||
![]() | Harper NJ / Burnside C / Klinge S | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Principles of mitoribosomal small subunit assembly in eukaryotes. 著者: Nathan J Harper / Chloe Burnside / Sebastian Klinge / ![]() 要旨: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is ...Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies. | |||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 228.6 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 55.6 KB 55.6 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 14.1 KB | 表示 | ![]() |
画像 | ![]() | 69.1 KB | ||
マスクデータ | ![]() | 244.1 MB | ![]() | |
Filedesc metadata | ![]() | 13.4 KB | ||
その他 | ![]() ![]() | 191.2 MB 189.9 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8csqMC ![]() 8cspC ![]() 8csrC ![]() 8cssC ![]() 8cstC ![]() 8csuC ![]() 8d8jC ![]() 8d8kC ![]() 8d8lC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Mitochondrial small subunit assembly intermediate (State A) | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.062 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Mitochondrial small subunit assembly intermediate (State A)
ファイル | emd_26967_half_map_1.map | ||||||||||||
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注釈 | Mitochondrial small subunit assembly intermediate (State A) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Mitochondrial small subunit assembly intermediate (State A)
ファイル | emd_26967_half_map_2.map | ||||||||||||
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注釈 | Mitochondrial small subunit assembly intermediate (State A) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Human mitochondrial small subunit assembly intermediate, State B
+超分子 #1: Human mitochondrial small subunit assembly intermediate, State B
+分子 #1: 28S ribosomal protein S34, mitochondrial
+分子 #2: 28S ribosomal protein S35, mitochondrial
+分子 #3: Aurora kinase A-interacting protein
+分子 #4: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
+分子 #5: Dimethyladenosine transferase 1, mitochondrial
+分子 #6: Methyltransferase-like protein 17, mitochondrial
+分子 #7: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
+分子 #9: 28S ribosomal protein S2, mitochondrial
+分子 #10: 28S ribosomal protein S24, mitochondrial
+分子 #11: 28S ribosomal protein S5, mitochondrial
+分子 #12: 28S ribosomal protein S6, mitochondrial
+分子 #13: 28S ribosomal protein S7, mitochondrial
+分子 #14: 28S ribosomal protein S9, mitochondrial
+分子 #15: 28S ribosomal protein S10, mitochondrial
+分子 #16: 28S ribosomal protein S12, mitochondrial
+分子 #17: 28S ribosomal protein S14, mitochondrial
+分子 #18: 28S ribosomal protein S15, mitochondrial
+分子 #19: 28S ribosomal protein S16, mitochondrial
+分子 #20: 28S ribosomal protein S17, mitochondrial
+分子 #21: 28S ribosomal protein S18b, mitochondrial
+分子 #22: 28S ribosomal protein S18c, mitochondrial
+分子 #23: 28S ribosomal protein S21, mitochondrial
+分子 #24: 28S ribosomal protein S22, mitochondrial
+分子 #25: 28S ribosomal protein S23, mitochondrial
+分子 #26: 28S ribosomal protein S25, mitochondrial
+分子 #27: 28S ribosomal protein S26, mitochondrial
+分子 #28: 28S ribosomal protein S27, mitochondrial
+分子 #29: 28S ribosomal protein S28, mitochondrial
+分子 #30: 28S ribosomal protein S29, mitochondrial
+分子 #31: 28S ribosomal protein S31, mitochondrial
+分子 #32: 28S ribosomal protein S33, mitochondrial
+分子 #33: GTPase Era, mitochondrial
+分子 #8: 12S mitochondrial rRNA
+分子 #34: IRON/SULFUR CLUSTER
+分子 #35: S-ADENOSYLMETHIONINE
+分子 #36: POTASSIUM ION
+分子 #37: MAGNESIUM ION
+分子 #38: ZINC ION
+分子 #39: FE2/S2 (INORGANIC) CLUSTER
+分子 #40: ADENOSINE-5'-TRIPHOSPHATE
+分子 #41: GUANOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 撮影したグリッド数: 1 / 実像数: 47037 / 平均電子線量: 51.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm 最小 デフォーカス(公称値): 0.7000000000000001 µm 倍率(公称値): 64000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
初期モデル |
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精密化 | 空間: REAL | ||||||||
得られたモデル | ![]() PDB-8csq: |