EMDB-27251: Yeast mitochondrial small subunit assembly intermediate (State 3)

Basic information

Entry

Database: EMDB / ID: EMD-27251

• Title: Yeast mitochondrial small subunit assembly intermediate (State 3)

Sample

• Complex: Yeast mitochondrial small subunit assembly intermediate (State 3)
  • Protein or peptide: x 34 types
  • RNA: x 1 types
• Ligand: x 4 types

• Keywords: Ribonucleoprotein complex Mitochondria Biogenesis / RIBOSOME

Function / homology

Function:

Branched-chain amino acid catabolism / mitochondrial small ribosomal subunit assembly / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / L-valine catabolic process / Mitochondrial protein degradation / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / methyltransferase activity / peroxisome / 4 iron, 4 sulfur cluster binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / mitochondrial inner membrane / rRNA binding / structural constituent of ribosome / ribosome / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding / cytoplasm

Domain/homology:

Ribosomal protein S22, mitochondrial, budding yeast / Ribosomal protein VAR1 / Mitochondrial ribosomal protein (VAR1) / : / : / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein S35, mitochondrial / Ribosomal protein MRP10, mitochondrial / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / Small ribosomal subunit protein mS41 SAM domain / IGR protein motif / IGR / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / CHCH / CHCH domain / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / ClpP/crotonase-like domain superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 2. / Ribosomal protein S14, conserved site / Ribosomal protein S14 signature. / Ribosomal protein S2 signature 1. / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S7, conserved site / Ribosomal protein S7 signature. / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S5, C-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein S13, conserved site / Ribosomal protein S13 signature. / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S13/S18 / Ribosomal protein S13 family profile. / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S4, conserved site / Ribosomal protein S15 signature. / Ribosomal protein S4 signature. / Ribosomal protein S4/S9 / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / S4 RNA-binding domain profile. / Ribosomal protein S10p/S20e

Component:

Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS43 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS47 / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein uS5m / Ribosome assembly protein RSM22, mitochondrial / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS41 / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS42 / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS19m / Small ribosomal subunit protein uS13m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS8m / Small ribosomal subunit protein mS35

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.6 Å
• Authors: Burnside C / Harper N / Klinge S

Funding support

United States, 2 items

Organization	Grant number	Country
Other private
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	5T32GM136640-02	United States

• Citation: Journal: Nature / Year: 2023; Title: Principles of mitoribosomal small subunit assembly in eukaryotes.; Authors: Nathan J Harper / Chloe Burnside / Sebastian Klinge / ; Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.

History

Deposition	Jun 8, 2022	-
Header (metadata) release	Dec 14, 2022	-
Map release	Dec 14, 2022	-
Update	Oct 9, 2024	-
Current status	Oct 9, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_27251.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.057 Å

Density

Contour Level	By AUTHOR: 3.46
Minimum - Maximum	-15.756205 - 39.186259999999997
Average (Standard dev.)	0.02194962 (±0.97032654)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 422.80002 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_27251_msk_1.map

Half map: #2

• File: emd_27251_half_map_1.map

Half map: #1

• File: emd_27251_half_map_2.map

Sample components

Entire : Yeast mitochondrial small subunit assembly intermediate (State 3)

• Entire: Name: Yeast mitochondrial small subunit assembly intermediate (State 3)

Components

• Complex: Yeast mitochondrial small subunit assembly intermediate (State 3)
  • Protein or peptide: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP13, mitochondrial
  • Protein or peptide: Ribosomal protein VAR1, mitochondrial
  • Protein or peptide: 37S ribosomal protein SWS2, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP2, mitochondrial
  • Protein or peptide: 37S ribosomal protein S28, mitochondrial
  • Protein or peptide: 37S ribosomal protein S16, mitochondrial
  • Protein or peptide: 37S ribosomal protein S17, mitochondrial
  • Protein or peptide: 37S ribosomal protein RSM18, mitochondrial
  • Protein or peptide: 37S ribosomal protein S19, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP21, mitochondrial
  • Protein or peptide: 37S ribosomal protein S25, mitochondrial
  • Protein or peptide: 37S ribosomal protein PET123, mitochondrial
  • Protein or peptide: 37S ribosomal protein S23, mitochondrial
  • Protein or peptide: Mitochondrial 37S ribosomal protein S27
  • Protein or peptide: 37S ribosomal protein S24, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP10, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP51, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP4, mitochondrial
  • Protein or peptide: Protein FYV4, mitochondrial
  • Protein or peptide: 37S ribosomal protein S26, mitochondrial
  • Protein or peptide: 37S ribosomal protein NAM9, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP1, mitochondrial
  • Protein or peptide: 37S ribosomal protein S5, mitochondrial
  • Protein or peptide: 37S ribosomal protein MRP17, mitochondrial
  • Protein or peptide: 37S ribosomal protein S35, mitochondrial
  • Protein or peptide: 37S ribosomal protein S7, mitochondrial
  • Protein or peptide: 37S ribosomal protein S8, mitochondrial
  • Protein or peptide: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
  • Protein or peptide: 37S ribosomal protein S9, mitochondrial
  • RNA: 15S ribosomal RNA
  • Protein or peptide: 37S ribosomal protein S10, mitochondrial
  • Protein or peptide: 37S ribosomal protein S18, mitochondrial
  • Protein or peptide: unknown protein sequence
  • Protein or peptide: 37S ribosomal protein S12, mitochondrial
• Ligand: IRON/SULFUR CLUSTER
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: water

Supramolecule #1: Yeast mitochondrial small subunit assembly intermediate (State 3)

• Supramolecule: Name: Yeast mitochondrial small subunit assembly intermediate (State 3); type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.26 MDa

Macromolecule #1: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase ...

• Macromolecule: Name: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial; type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 72.308328 KDa

Sequence

String:

MMKRCFSILP QNVRFSSKFT SLNLPKLDLA DFIDSNKRGI NVLPSYRDET ASTTQATNSK ELRLLSKTLQ GQSYRDQLEL NPDVSKAIN NNIMAVHIPN NLRRVATNYY KEIQEPNSLH RPCRTKMEVD AHIASIFLQN YGSIFQSLKE LQKRVGPDNF K PQRILDVG YGPATGIVAL NDILGPNYRP DLKDAVILGN AEMQERAKII LSRQLNEVVD TVEENVSTEK EQETDRRNKN FQ EDEHIGE VMTKKINIMT NLRSSIPASK EYDLIILTHQ LLHDGNQFPI QVDENIEHYL NILAPGGHIV IIERGNPMGF EII ARARQI TLRPENFPDE FGKIPRPWSR GVTVRGKKDA ELGNISSNYF LKVIAPCPHQ RKCPLQVGNP NFYTHKEGKD LKFC NFQKS IKRPKFSIEL KKGKLLATSW DGSQGNASRL KGTGRRNGRD YEILNYSYLI FERSHKDENT LKEIKKLRNE NVNGK YDIG SLGDDTQNSW PRIINDPVKR KGHVMMDLCA PSGELEKWTV SRSFSKQIYH DARKSKKGDL WASAAKTQIK GLGDLN VKK FHKLEKERIK QLKKEERQKA RKAMESYNEL EDSLQFDDHQ FSNFEVMKKL STFHGNDFLQ HVNRK

UniProtKB: Ribosome assembly protein RSM22, mitochondrial

Macromolecule #2: 37S ribosomal protein MRP13, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP13, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 39.045031 KDa

Sequence

String:

MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK LKPFIYRPKN AQILLTKDIR DPKTREPLQ PRPPVKPLSK QTLNDFIYSV EPNSTELLDW FKEWTGTSIR KRAIWTYISP IHVQKMLTAS FFKIGKYAHM V GLLYGIEH KFLKAQNPSV FDIEHFFNTN IMCALHRNRL KDYKDAEIAQ RKLQVAWKKV LNRKNNTGLA NILVATLGRQ IG FTPELTG LQPVDISLPD IPNSSSGAEL KDLLSKYEGI YLIARTLLDI DQHNAQYLEL QEFIRQYQNA LSESSDPYDT HLK ALGLLE TPPPQESTEK EEK

UniProtKB: Small ribosomal subunit protein mS27

Macromolecule #3: Ribosomal protein VAR1, mitochondrial

• Macromolecule: Name: Ribosomal protein VAR1, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 47.170996 KDa

Sequence

String:

MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN NMNPAGANPV VHRIGPAGNI NNKLQHLNN MNNWNTQIYN YNKNMEIMNT MNDKLINKLL YKMMTLKLNN MNINKIIMSK TINQHSLNKL NIKFYYYNND I NNNNNNNN NNYYMNMMNK LMNIMNNNMN NNLCNILSYY YKKKVTIEPI KLSYIYLNSD IFSKYISLND MDKYNNGILT NY QRMLNNI MPKLNDHNIS MNYINNINNI NNNKYNNMIN LLNNNNNINN NNNYNNNNNN YIGNINNIYN NMTIDNIPMD ILM YKYLVG WSIKFKGRLS NNNGRTSTTN LLNGTFNNKK YLWSNINNNY KLNYIPSNHN LYNNSNINKN GKYNIKVKLN FI

UniProtKB: Small ribosomal subunit protein uS3m

Macromolecule #4: 37S ribosomal protein SWS2, mitochondrial

• Macromolecule: Name: 37S ribosomal protein SWS2, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.121047 KDa

Sequence

String:

MVVHILGKGF KGKEVIKIAL ASKFYGIGKT TAEKICSKLG FYPWMRMHQL SEPQIMSIAS ELSTMTIEGD ARAIVKDNIA LKRKIGSYS GMRHTLHLPV RGQHTRNNAK TARKLNKIDR RGIHTFSQAK VQHNPSLWSC IFGK

UniProtKB: Small ribosomal subunit protein uS13m

Macromolecule #5: 37S ribosomal protein MRP2, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP2, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.563061 KDa

Sequence

String:

MGNFRFPIKT KLPPGFINAR ILRDNFKRQQ FKENEILVKS LKFIARNMNL PTKLRLEAQL KLNALPNYMR STQIKNRCVD SGHARFVLS DFRLCRYQFR ENALKGNLPG VKKGIW

UniProtKB: Small ribosomal subunit protein uS14m

Macromolecule #6: 37S ribosomal protein S28, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.116383 KDa

Sequence

String:

MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS TDKVDPVLGR ADTPFITRIM AELKEPLVL SKGYNIEEVD KFLAAIESAK RERAELSGLN TEVVGIEDIE KLEDRREAIL RILSMRNSEN KNAIKMAVEL A RKEFERFP GDTGSSEVQA ACMTVRIQNM ANHIKEHRKD FANTRNLRIL VQQRQAILRY LKRDNPEKYY WTIQKLGLND AA ITDEFNM DRRYMQDYEF FGDKILIRDS KKVANQKRKE IRKQKRATF

UniProtKB: Small ribosomal subunit protein uS15m

Macromolecule #7: 37S ribosomal protein S16, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.663011 KDa

Sequence

String:

MTCGLVRIRL ARFGRKNSPV YNIVVANSRK ARDAKPIEVL GTYVPVPSPV TKRELKRGVV PIKDVKLDFD RTKYWIGVGA QPSETVTKL LRKAGILNDA WATSKNSNVN RKVVFERMET LE

UniProtKB: Small ribosomal subunit protein bS16m

Macromolecule #8: 37S ribosomal protein S17, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.683986 KDa

Sequence

String:

MARQNFLGLV VSQGKMQKTV KVRVETKVFN KKINKELFHR RDYLVHDEGE ISREGDLVRI EATRPLSKRK FFAIAEIIRN KGQQFALYE SEAQLSVAKE EAQKAKEFLD KRSVRENKLN EKTTLLRDIR TIQDALSSGS TPKELLEIKQ RYGIQDFSQE T VRQLLQLD ISGLEVNLEK QRSLIDRIQT RLSELLSNDL KCDQFLKDHG VEDPLTLKKN IKKNLLRKHV MMDMQQPSQ

UniProtKB: Small ribosomal subunit protein uS17m

Macromolecule #9: 37S ribosomal protein RSM18, mitochondrial

• Macromolecule: Name: 37S ribosomal protein RSM18, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.86649 KDa

Sequence

String:

MQPIIKGAVS STFKRALYNF GIKEKKSVNI EMGRTQQTKK IDQSLSKKLP KGTIYDPFDF SMGRIHLDRK YQANKNSNRN DIMKSGANP LEFYARPRIL SRYVTSTGRI QHRDITGLSA KNQRRLSKAI RRCQAIGLM

UniProtKB: Small ribosomal subunit protein bS18m

Macromolecule #10: 37S ribosomal protein S19, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S19, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.292044 KDa

Sequence

String:

MQPAARLLSR SVWKGPNIVP LPIREAMTKG TPIRTNARAA TILPQFVGLK FQIHNGKEYV PIEISEDMVG HKLGEFAPTR KRFSYTQTK NK

UniProtKB: Small ribosomal subunit protein uS19m

Macromolecule #11: 37S ribosomal protein MRP21, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP21, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.432744 KDa

Sequence

String:

MLKSTLRLSR ISLRRGFTTI DCLRQQNSDI DKIILNPIKL AQGSNSDRGQ TSKSKTDNAD ILSMEIPVDM MQSAGRINKR ELLSEAEIA RSSVENAQMR FNSGKSIIVN KNNPAESFKR LNRIMFENNI PGDKRSQRFY MKPGKVAELK RSQRHRKEFM M GFKRLIEI VKDAKRKGY

UniProtKB: Small ribosomal subunit protein bS21m

Macromolecule #12: 37S ribosomal protein S25, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 30.554354 KDa

Sequence

String:

MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL VDVTEQPHAN NKGLYKTRPN SSDKRVGVK RLYRPPKLTY VEDRLRSLFY KQHPWELSRP KILVENEIGD ENYDWSHMLQ IGRPLDGESV IQRTMYLIKT K QYGDMVEA YDHARYEFYA LRMQEETEQQ VALEEAEMFG SLFGVSAIEH GIQKEQEVLD VWEKKVVEET ELMAARTSNP AG SWKDDTT LNTAQEEEST TSENLHF

UniProtKB: Small ribosomal subunit protein mS23

Macromolecule #13: 37S ribosomal protein PET123, mitochondrial

• Macromolecule: Name: 37S ribosomal protein PET123, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 36.059281 KDa

Sequence

String:

MGKGAAKYGF KSGVFPTTRS ILKSPTTKQT DIINKVKSPK PKGVLGIGYA KGVKHPKGSH RLSPKVNFID VDNLIAKTVA EPQSIKSSN GSAQKVRLQK AELRRKFLIE AFRKEEARLL HKHEYLQKRT KELEKAKELE LEKLNKEKSS DLTIMTLDKM M SQPLLRNR SPEESELLKL KRNYNRSLLN FQAHKKKLNE LLNLYHVANE FIVTESQLLK KIDKVFNDET EEFTDAYDVT SN FTQFGNR KLLLSGNTTL QTQINNAIMG SLSNEKFFDI SLVDSYLNKD LKNISNKIDS KLNPTSNGAG NNGNNNNTTN L

UniProtKB: Small ribosomal subunit protein mS26

Macromolecule #14: 37S ribosomal protein S23, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 50.940742 KDa

Sequence

String:

MLRMSTSRFI GQRLFTTARS LQAAKPAPKG KTQGFSKKSS SVSSYSSAKR VTPGSLYKNW TNTTHTAQLQ QTAVPLALPI FNFDDISKT LNKVVSYSNK QYKSLHHLGS FKKSQFNELF QKPVCLVRED ATNSFLKKLV SHPVKKFIIT GEPGVGKTVL L SQAHAYAV DSKQIIINIS YPELFLNGRN DFSYDDDLKL FIQPMYLKKL IRKILKANDP ALLKSIELSK DYKFSNANPK NA SVKPFVT LNKTKNTVLD LLSVMTHPHN RGKLMKAIID ELSVQSKVPI MFTVDNFSKV LTTAYSAYRN TENKQIYSLD LQM GKLMMD IISGETKFAN GESSTILAIS GVDRTNKTLP VALGKIPVDP YVTRYHYEPK FVELLQKGNV TEFEVPKLNK QEVN ELIDY YKQSNVLLDK DITGKKWENL IDEKYFLSGN GNPRELLKSL VLSHR

UniProtKB: Small ribosomal subunit protein mS29

Macromolecule #15: Mitochondrial 37S ribosomal protein S27

• Macromolecule: Name: Mitochondrial 37S ribosomal protein S27 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.420666 KDa

Sequence

String:

MNVPKARLLK VAELSAKIFD QNFNPSGIRT GSKILNERLK GPSVASYYGN PDILKFRHLK TLYPDIEFVD LEEQYRLSMV EAKKRRGKG APKKMKKDAA ATAKGKGKKK K

UniProtKB: Small ribosomal subunit protein mS33

Macromolecule #16: 37S ribosomal protein S24, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 37.458496 KDa

Sequence

String:

MKVPLGLWKV SRGNLWSTQK RVLTMSRCLN SDAGNEAKTV REGPAFSADL YMHPEKWKGL PPQRILELYW ERMARLGSEY KPNKDELNA LLTTSEYSNV PVNDIKKLYH RGEQGAIDIK GGNVNRDNSL RPFMFDELPS QAQELVAQHR EQRFYNRLAA Y ELPLLAQY RQEYKRPSPE SHPVTYRYTS YVGEEHPNSR KVVLSVKTKE LGLEEKSLHK FRILARSRYD HTTDIFKMSS DK FEHASQN ARYLHDILQR LLAESKDLTE DDFSDVPLDT RHTIAKSLRK KKRDYEFPEH WKRPEDAPKK KFDIVDQLLS TL

UniProtKB: Small ribosomal subunit protein mS35

Macromolecule #17: 37S ribosomal protein MRP10, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP10, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.709598 KDa

Sequence

String:

MSGKPPVYRL PPLPRLKVKK PIIRQEANKC LVLMSNLLQC WSSYGHMSPK CAGLVTELKS CTSESALGKR NNVQKSNINY HAARLYDRI NGKPHD

UniProtKB: Small ribosomal subunit protein mS37

Macromolecule #18: 37S ribosomal protein MRP51, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP51, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 39.507457 KDa

Sequence

String:

MTLAELLGRS RIAQVANNHK PLTYTGKKFH PTHQIIETKP STLYRQEWGL KSAIPSKIKS RYLVYNDLDT LERITTFEPR GGTQWNRLR FQEMGVPIVS NIGRQNPFFK YISRPEDESH AKLSLFKEMK GDTDISPAAM KKRLKKITAL IRSFQDEFKE W LVENHPDE LKLNSNKLED YVVKFLNKKL ETKTNKKFNT EIIGTGGLSY SLPGKLKNSP NGVIQRTVVP GRILNVVKEN ND NKWLAAI GGFVADVVFF QSPPSSFNSM GDFIRMKTFL FEILEASMEK NGSVSMHARL LEPQNDKTRE FFNKRPIYKP LTS RRARRP SVGNIQEANN LLNIIKGN

UniProtKB: Small ribosomal subunit protein bS1m

Macromolecule #19: 37S ribosomal protein MRP4, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP4, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 44.205727 KDa

Sequence

String:

MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS LSQVPEDEIL PELKKSLEQD KLSDKEQQL EAELSDFFRN YALLNKLFDS KTLDGQSSTT TAAATPTKPY PNLIPSANDK PYSSQELFLR QLNHSMRTAK L GATISKVY YPHKDIFYPP LPENITVESL MSAGVHLGQS TSLWRSSTQS YIYGEYKGIH IIDLNQTLSY LKRAAKVVEG VS ESGGIIL FLGTRQGQKR GLEEAAKKTH GYYVSTRWIP GTLTNSTEIS GIWEKQEIDS NDNPTERALS PNETSKQVKP DLL VVLNPT ENRNALLEAI KSRVPTIAII DTDSEPSLVT YPIPGNDDSL RSVNFLLGVL ARAGQRGLQN RLARNNEK

UniProtKB: Small ribosomal subunit protein uS2m

Macromolecule #20: Protein FYV4, mitochondrial

• Macromolecule: Name: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.321684 KDa

Sequence

String:

MIPSRISHKF PLFLRSSLAA PKAAYRFSST IPKPSDQVPD VDAFLNKIGR NCNELKDTFE NNWNNLFQWD SKILKEKGVN IQQRKYILK QVHNYRNNRP IHEIKLGKKS FFGGERKRKA FTAKWKAENK Q

UniProtKB: Small ribosomal subunit protein mS41

Macromolecule #21: 37S ribosomal protein S26, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 30.257449 KDa

Sequence

String:

MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES YYPFHILLKT AGNPLQSNIF NLASSIHNN HLFVENILPS AVEHGTNSNA VVKTEPSRLF LSKIKDSFNG SDWEVVKEEM IYRAENEVLG QGWLFLVENN E KKLFILTS NNNGTPYYFP RNQSFDLNSA ISIDEFATLK QMKELIGKST KLNGKVQDWT MPIICVNLWD HAYLHDYGVG NR SKYVKNV LDNLNWSVVN NRIFSGISK

UniProtKB: Small ribosomal subunit protein mS42

Macromolecule #22: 37S ribosomal protein NAM9, mitochondrial

• Macromolecule: Name: 37S ribosomal protein NAM9, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.450949 KDa

Sequence

String:

MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIK ETPFLLQTFA VLEKRLDFAL FRAMFASSVR QARQFILHGN VRVNGVKIKH PSYTLKPGDM FSVKPDKVLE A LGAKKPSF QEALKIDKTQ IVLWNKYVKE AKTEPKEVWE KKLENFEKMS DSNPKKLQFQ EFLRQYNKNL ESQQYNALKG CT QEGILRK LLNVEKEIGK SNNEPLSIDE LKQGLPEIQD SQLLESLNNA YQEFFKSGEI RREIISKCQP DELISLATEM MNP NETTKK ELSDGAKSAL RSGKRIIAES VKLWTKNITD HFKTRMSDIS DGSLTFDPKW AKNLKYHDPI KLSELEGDEP KARK LINLP WQKNYVYGRQ DPKKPFFTPW KPRPFLSPFA ILPHHLEISF KTCHAVYLRD PVARPGQSEV ISPFDVPVHE RAYMY YLRN GK

UniProtKB: Small ribosomal subunit protein uS4m

Macromolecule #23: 37S ribosomal protein MRP1, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP1, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 36.775781 KDa

Sequence

String:

MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ QKPLETLIHE NSKSASKKHI VNYASSLYN LKFSMSSLQG CIRTPPEECP RLGPEALLQT PDFNRTISNE PLTTGNERLQ AALISSFGSL MEFRTLLINS N LAISGDGF TWLVARRQLD KRAMRNDMPN RDIEYDKLFI LNTYNAGTPF NFSTSGVMNE LNNQYTNMEK QRAKEAGNLE DS EMTAKQA KTKFIYETQQ KGFSGKEVSY IPLLAIDASP KTWLTDYGVF GKREYLERVW DSIEWKIVES RLPQRTKIQA FNT L

UniProtKB: Small ribosomal subunit protein mS43

Macromolecule #24: 37S ribosomal protein S5, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.941102 KDa

Sequence

String:

MFKRQLSTSV RYLQHYDESL LSRYYPESLL KSIKLAQQTI PEDTKFRVSR NVEFAPPYLD DFTKIHPFWD YKPGMPHLHA QEENNNFSI FRWDQVQQPL PGEGNILPPG VSLPNDGGRK SKSADVAAGL HKQTGVDPDY ITRKLTMKPL VMKRVSNQTG K GKIASFYA LVVVGDKNGM VGLGEGKSRE EMSKAIFKAH WDAVRNLKEI PRYENRTIYG DIDFRYHGVK LHLRSAKPGF GL RVNHVIF EICECAGIKD LSGKVYKSRN DMNIAKGTIE AFTKAQKTLD EVALGRGKKL VDVRKVYYSS

UniProtKB: Small ribosomal subunit protein uS5m

Macromolecule #25: 37S ribosomal protein MRP17, mitochondrial

• Macromolecule: Name: 37S ribosomal protein MRP17, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.046702 KDa

Sequence

String:

MLYELIGLVR ITNSNAPKLE AKELSSTIGK LIIQNRGVVR DIVPMGIRYL PKIMKKDQEK HFRAYHFLML FDSSAAVQSE ILRTLKKDP RVIRSSIVKV DLDKQLDRAS SLHRSLGKKS ILELVNEDYQ SI

UniProtKB: Small ribosomal subunit protein bS6m

Macromolecule #26: 37S ribosomal protein S35, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 39.638172 KDa

Sequence

String:

MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ FLGPKNYKGE YVMNKYFTVP TNHVPNYIK PDLERGQSLE HPVTKKPLQL RYDGTLGPPP VENKRLQNIF KDRLLQPFPS NPHCKTNYVL SPQLKQSIFE E ITVEGLSA QQVSQKYGLK IPRVEAIVKL VSVENSWNRR NRVSSDLKTM DETLYRMFPV FDSDASFKRE NLSEIPVPQK TL ASRFLTI AESEPFGPVD AAHVLELEPA VETLRNLSTV GEHSSGHQQS TNKNTKVIYG ELVEGERSQY KFTNAKVGKV GYR YGSGNR DNKKDRRIGF NKLGQMVYI

UniProtKB: Small ribosomal subunit protein mS45

Macromolecule #27: 37S ribosomal protein S7, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.857141 KDa

Sequence

String:

MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK EYLPETSLAP PGQSKVDLLQ GSQAGSKIK PTAEQLAQWE ALKSVPIPPR KNATLDHITN MIMRHGKKEK AQTILSRALY LVYCQTRQDP IQALEKSLDE L APLMMTKT FNTGVAKASV IPVPLNKRQR NRIAWNWIVQ SANQRVSSDF AVRLGEELTA IAKGTSSAFE KRDQIHKTAI AH RAYIQLK

UniProtKB: Small ribosomal subunit protein uS7m

Macromolecule #28: 37S ribosomal protein S8, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S8, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.496602 KDa

Sequence

String:

MSLVKLANTC AHLQNCSKVR VALTSIPYTK LQLQFAYNLY QQGFLSSLQK GSTMGPDKDF VEVTPDNIST RRLWVGLKYR DNKPVLSSC KLISKPNSRI HLPMEDMKKL CSGVTIRNIK PLQPGELILV RAHNNIMDIN EAISKKLDGE VLCRVK

UniProtKB: Small ribosomal subunit protein uS8m

Macromolecule #29: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

• Macromolecule: Name: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO / EC number: 3-hydroxyisobutyryl-CoA hydrolase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.351195 KDa

Sequence

String:

MLRNTLKCAQ LSSKYGFKTT TRTFMTTQPQ LNVTDAPPVL FTVQDTARVI TLNRPKKLNA LNAEMSESMF KTLNEYAKSD TTNLVILKS SNRPRSFCAG GDVATVAIFN FNKEFAKSIK FFTDEYSLNF QIATYLKPIV TFMDGITMGG GVGLSIHTPF R IATENTKW AMPEMDIGFF PDVGSTFALP RIVTLANSNS QMALYLCLTG EVVTGADAYM LGLASHYVSS ENLDALQKRL GE ISPPFNN DPQSAYFFGM VNESIDEFVS PLPKDYVFKY SNEKLNVIEA CFNLSKNGTI EDIMNNLRQY EGSAEGKAFA QEI KTKLLT KSPSSLQIAL RLVQENSRDH IESAIKRDLY TAANMCMNQD SLVEFSEATK HKLIDKQRVP YPWTKKEQLF VSQL TSITS PKPSLPMSLL RNTSNVTWTQ YPYHSKYQLP TEQEIAAYIE KRTNDDTGAK VTEREVLNHF ANVIPSRRGK LGIQS LCKI VCERKCEEVN DGLRWK

UniProtKB: Small ribosomal subunit protein mS47

Macromolecule #30: 37S ribosomal protein S9, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 31.980258 KDa

Sequence

String:

MFSRLSLFRR AALAPAPMRM SFRTIYQKTE DELPRRIVPK LATFYSANPN HEDRINRLER LLRKYIKLPS QNNNEAQQTK APWISFDEY ALIGGGTKLK PTQYTQLLYM LNKLHNIDPQ LTNDEITSEL SQYYKKSSML SNNIKIKTLD EFGRSIAVGK R KSSTAKVF VVRGTGEILV NGRQLNDYFL KMKDRESIMY PLQVIESVGK YNIFATTSGG GPTGQAESIM HAIAKALVVF NP LLKSRLH KAGVLTRDYR HVERKKPGKK KARKMPTWVK R

UniProtKB: Small ribosomal subunit protein uS9m

Macromolecule #32: 37S ribosomal protein S10, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 23.45892 KDa

Sequence

String:

MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD FILRTGYYLG IPLTGPKPLP TRRERWTVI KSPFVHAKSK ENFERHTHKR LIRAWDTNPE VLQMLIAYIT KHSMAGVGMK CNFFQRSEIS LDLGSDANGL E KSLSNIDE LYSLRNDDKA QTSAVGQKVL ELLDSPDFKK HLEKK

UniProtKB: Small ribosomal subunit protein uS10m

Macromolecule #33: 37S ribosomal protein S18, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S18, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.60342 KDa

Sequence

String:

MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS NGSSEVYKPK EEVVKYILHG KFTKNNTHL TFSSVVEDKN FHKNKGLTYN DTMLYYLNLP QKVKISLSTG CLGFRKAARG EYEAAFQTSG RMFELIKEKN M LNKDIEVV MDDFGKGRAA FISALVGKEG ASVVKKVVKI SDATKLKFGG VRSPKMRRL

UniProtKB: Small ribosomal subunit protein uS11m

Macromolecule #34: unknown protein sequence

• Macromolecule: Name: unknown protein sequence / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.017875 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #35: 37S ribosomal protein S12, mitochondrial

• Macromolecule: Name: 37S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.94874 KDa

Sequence

String:

MLSRFMSNTW CTPLRQAQRL FSSTTTMQAT LNQIKRGSGP PRRKKISTAP QLDQCPQRKG VVLRVMVLKP KKPNSAQRKA CRVRLTNGN VVSAYIPGEG HDAQEHSIVY VRGGRCQDLP GVKYHVIRGA GDLSGVVNRI SSRSKYGAKK PSKS

UniProtKB: Small ribosomal subunit protein uS12m

Macromolecule #31: 15S ribosomal RNA

• Macromolecule: Name: 15S ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 549.234062 KDa

Sequence

String:

UUUUAUAUAA UAAUAAUAAU AUAUAUAUAU AUAUAUUAUU AUAUUAGUUA UAUAAUAAGG AAAAGUAAAA AAUUUAUAAG AAUAUGAUG UUGGUUCAGA UUAAGCGCUA AAUAAGGACA UGACACAUGC GAAUCAUACG UUUAUUAUUG AUAAGAUAAU A AAUAUGUG GUGUAAACGU GAGUAAUUUU AUUAGGAAUU AAUGAACUAU AGAAUAAGCU AAAUACUUAA UAUAUUAUUA UA UAAAAAU AAUUUAUAUA AUAAAAAGGA UAUAUAUAUA AUAUAUAUUU AUCUAUAGUC AAGCCAAUAA UGGUUUAGGU AGU AGGUUU AUUAAGAGUU AAACCUAGCC AACGAUCCAU AAUCGAUAAU GAAAGUUAGA ACGAUCACGU UGACUCUGAA AUAU AGUCA AUAUCUAUAA GAUACAGCAG UGAGGAAUAU UGGACAAUGA UCGAAAGAUU GAUCCAGUUA CUUAUUAGGA UGAUA UAUA AAAAUAUUUU AUUUUAUUUA UAAAUAUUAA AUAUUUAUAA UAAUAAUAAU AAUAAUAUAU AUAUAUAAAU UGAUUA AAA AUAAAAUCCA UAAAUAAUUA AAAUAAUGAU AUUAAUUACC AUAUAUAUUU UUAUAUGGAU AUAUAUAUUA AUAAUAA UA UUAAUUUUAU UAUUAUUAAU AAUAUAUUUU AAUAGUCCUG ACUAAUAUUU GUGCCAGCAG UCGCGGUAAC ACAAAGAG G GCGAGCGUUA AUCAUAAUGG UUUAAAGGAU CCGUAGAAUG AAUUAUAUAU UAUAAUUUAG AGUUAAUAAA AUAUAAUUA AAGAAUUAUA AUAGUAAAGA UGAAAUAAUA AUAAUAAUUA UAAGACUAAU AUAUGUGAAA AUAUUAAUUA AAUAUUAACU GACAUUGAG GGAUUAAAAC UAGAGUAGCG AAACGGAUUC GAUACCCGUG UAGUUCUAGU AGUAAACUAU GAAUACAAUU A UUUAUAAU AUAUAUUAUA UAUAAAUAAU AAAUGAAAAU GAAAGUAUUC CACCUGAAGA GUACGUUAGC AAUAAUGAAA CU CAAAACA AUAGACGGUU ACAGACUUAA GCAGUGGAGC AUGUUAUUUA AUUCGAUAAU CCACGACUAA CCUUACCAUA UUU UGAAUA UUAUAAUAAU UAUUAUAAUU AUUAUAUUAC AGGCGUUACA UUGUUGUCUU UAGUUCGUGC UGCAAAGUUU UAGA UUAAG UUCAUAAACG AACAAAACUC CAUAUAUAUA AUUUUAAUUA UAUAUAAUUU UAUAUUAUUU AUUAAUAUAA AGAAA GGAA UUAAGACAAA UCAUAAUGAU CCUUAUAAUA UGGGUAAUAG ACGUGCUAUA AUAAAAUGAU AAUAAAAUUA UAUAAA AUA UAUUUAAUUA UAUUUAAUUA AUAAUAUAAA ACAUUUUAAU UUUUAAUAUA UUUUUUUAUU AUAUAUUAAU AUGAAUU AU AAUCUGAAAU UCGAUUAUAU GAAAAAAGAA UUGCUAGUAA UACGUAAAUU AGUAUGUUAC GGUGAAUAUU CUAACUGU U UCGCACUAAU CACUCAUCAC GCGUUGAAAC AUAUUAUUAU CUUAUUAUUU AUAUAAUAUU UUUUAAUAAA UAUUAAUAA UUAUUAAUUU AUAUUUAUUU AUAUCAGAAA UAAUAUGAAU UAAUGCGAAG UUGAAAUACA GUUACCGUAG GGGAACCUGC GGUGGGCUU AUAAAUAUCU UAAAUAUUCU UACA

GENBANK: GENBANK: CP059539.1

Macromolecule #36: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 36 / Number of copies: 1 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #37: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 37 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #38: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 72 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #39: water

• Macromolecule: Name: water / type: ligand / ID: 39 / Number of copies: 3 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 14111 / Average electron dose: 61.73 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Calibrated magnification: 64000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 3544843
• Startup model: Type of model: OTHER / Details: Ab Initio (Cryosparc)
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: PHENIX (ver. 1.19); Details: 2 focused maps calculated in RELION 3.1.1 were combined into a composite map using phenix.combine_focused_maps. Composite half maps were generated by combining each half map from multi body refinements.; Number images used: 381285
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)

Atomic model buiding 1

Initial model

PDB ID:

5mrc

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL

Output model

PDB-8d8l:
Yeast mitochondrial small subunit assembly intermediate (State 3)