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Yorodumi- EMDB-25449: RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displayi... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-25449 | |||||||||
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Title | RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displaying the SARS-CoV-2 Receptor-Binding Domain | |||||||||
Map data | RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displaying the SARS-CoV-2 Receptor-Binding Domain | |||||||||
Sample |
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Keywords | vaccine / nanoparticle / SARS-CoV-2 / RBD / VIRUS LIKE PARTICLE | |||||||||
Biological species | Severe acute respiratory syndrome coronavirus 2 | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 30.0 Å | |||||||||
Authors | Thomas PV / Smith C / Chen WH / Sankhala RS / Hajduczki A / Choe M / Martinez E / Chang W / Peterson CE / Karch C ...Thomas PV / Smith C / Chen WH / Sankhala RS / Hajduczki A / Choe M / Martinez E / Chang W / Peterson CE / Karch C / Gohain N / Kannadka CB / de Val N / Joyce MG / Modjarrad K | |||||||||
Funding support | United States, 2 items
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Citation | Journal: bioRxiv / Year: 2021 Title: SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity. Authors: M Gordon Joyce / Wei-Hung Chen / Rajeshwer S Sankhala / Agnes Hajduczki / Paul V Thomas / Misook Choe / William Chang / Caroline E Peterson / Elizabeth Martinez / Elaine B Morrison / Clayton ...Authors: M Gordon Joyce / Wei-Hung Chen / Rajeshwer S Sankhala / Agnes Hajduczki / Paul V Thomas / Misook Choe / William Chang / Caroline E Peterson / Elizabeth Martinez / Elaine B Morrison / Clayton Smith / Aslaa Ahmed / Lindsay Wieczorek / Alexander Anderson / Rita E Chen / James Brett Case / Yifan Li / Therese Oertel / Lorean Rosado / Akshaya Ganesh / Connor Whalen / Joshua M Carmen / Letzibeth Mendez-Rivera / Christopher Karch / Neelakshi Gohain / Zuzana Villar / David McCurdy / Zoltan Beck / Jiae Kim / Shikha Shrivastava / Ousman Jobe / Vincent Dussupt / Sebastian Molnar / Ursula Tran / Chandrika B Kannadka / Michelle Zemil / Htet Khanh / Weimin Wu / Matthew A Cole / Debra K Duso / Larry W Kummer / Tricia J Lang / Shania E Muncil / Jeffrey R Currier / Shelly J Krebs / Victoria R Polonis / Saravanan Rajan / Patrick M McTamney / Mark T Esser / William W Reiley / Morgane Rolland / Natalia de Val / Michael S Diamond / Gregory D Gromowski / Gary R Matyas / Mangala Rao / Nelson L Michael / Kayvon Modjarrad / Abstract: The need for SARS-CoV-2 next-generation vaccines has been highlighted by the rise of variants of concern (VoC) and the long-term threat of other coronaviruses. Here, we designed and characterized ...The need for SARS-CoV-2 next-generation vaccines has been highlighted by the rise of variants of concern (VoC) and the long-term threat of other coronaviruses. Here, we designed and characterized four categories of engineered nanoparticle immunogens that recapitulate the structural and antigenic properties of prefusion Spike (S), S1 and RBD. These immunogens induced robust S-binding, ACE2-inhibition, and authentic and pseudovirus neutralizing antibodies against SARS-CoV-2 in mice. A Spike-ferritin nanoparticle (SpFN) vaccine elicited neutralizing titers more than 20-fold higher than convalescent donor serum, following a single immunization, while RBD-Ferritin nanoparticle (RFN) immunogens elicited similar responses after two immunizations. Passive transfer of IgG purified from SpFN- or RFN-immunized mice protected K18-hACE2 transgenic mice from a lethal SARS-CoV-2 virus challenge. Furthermore, SpFN- and RFN-immunization elicited ACE2 blocking activity and neutralizing ID50 antibody titers >2,000 against SARS-CoV-1, along with high magnitude neutralizing titers against major VoC. These results provide design strategies for pan-coronavirus vaccine development. HIGHLIGHTS: Iterative structure-based design of four Spike-domain Ferritin nanoparticle classes of immunogensSpFN-ALFQ and RFN-ALFQ immunization elicits potent neutralizing activity against SARS-CoV- ...HIGHLIGHTS: Iterative structure-based design of four Spike-domain Ferritin nanoparticle classes of immunogensSpFN-ALFQ and RFN-ALFQ immunization elicits potent neutralizing activity against SARS-CoV-2, variants of concern, and SARS-CoV-1Passively transferred IgG from immunized C57BL/6 mice protects K18-hACE2 mice from lethal SARS-CoV-2 challenge. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_25449.map.gz | 9.6 MB | EMDB map data format | |
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Header (meta data) | emd-25449-v30.xml emd-25449.xml | 12.7 KB 12.7 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_25449_fsc.xml | 5.9 KB | Display | FSC data file |
Images | emd_25449.png | 17.8 KB | ||
Filedesc metadata | emd-25449.cif.gz | 4.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-25449 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-25449 | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_25449.map.gz / Format: CCP4 / Size: 15.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displaying the SARS-CoV-2 Receptor-Binding Domain | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.39 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displayi...
Entire | Name: RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displaying the SARS-CoV-2 Receptor-Binding Domain |
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Components |
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-Supramolecule #1: RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displayi...
Supramolecule | Name: RFN_131, a Ferritin-based Nanoparticle Vaccine Candidate Displaying the SARS-CoV-2 Receptor-Binding Domain type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Molecular weight | Theoretical: 1.1 MDa |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.05 mg/mL |
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Buffer | pH: 7.4 / Details: 1x PBS |
Staining | Type: NEGATIVE / Material: Uranyl Formate |
-Electron microscopy
Microscope | FEI TECNAI 20 |
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Image recording | Film or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: LAB6 |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |