National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01 AI157155
United States
Citation
Journal: bioRxiv / Year: 2021 Title: SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity. Authors: M Gordon Joyce / Wei-Hung Chen / Rajeshwer S Sankhala / Agnes Hajduczki / Paul V Thomas / Misook Choe / William Chang / Caroline E Peterson / Elizabeth Martinez / Elaine B Morrison / Clayton ...Authors: M Gordon Joyce / Wei-Hung Chen / Rajeshwer S Sankhala / Agnes Hajduczki / Paul V Thomas / Misook Choe / William Chang / Caroline E Peterson / Elizabeth Martinez / Elaine B Morrison / Clayton Smith / Aslaa Ahmed / Lindsay Wieczorek / Alexander Anderson / Rita E Chen / James Brett Case / Yifan Li / Therese Oertel / Lorean Rosado / Akshaya Ganesh / Connor Whalen / Joshua M Carmen / Letzibeth Mendez-Rivera / Christopher Karch / Neelakshi Gohain / Zuzana Villar / David McCurdy / Zoltan Beck / Jiae Kim / Shikha Shrivastava / Ousman Jobe / Vincent Dussupt / Sebastian Molnar / Ursula Tran / Chandrika B Kannadka / Michelle Zemil / Htet Khanh / Weimin Wu / Matthew A Cole / Debra K Duso / Larry W Kummer / Tricia J Lang / Shania E Muncil / Jeffrey R Currier / Shelly J Krebs / Victoria R Polonis / Saravanan Rajan / Patrick M McTamney / Mark T Esser / William W Reiley / Morgane Rolland / Natalia de Val / Michael S Diamond / Gregory D Gromowski / Gary R Matyas / Mangala Rao / Nelson L Michael / Kayvon Modjarrad / Abstract: The need for SARS-CoV-2 next-generation vaccines has been highlighted by the rise of variants of concern (VoC) and the long-term threat of other coronaviruses. Here, we designed and characterized ...The need for SARS-CoV-2 next-generation vaccines has been highlighted by the rise of variants of concern (VoC) and the long-term threat of other coronaviruses. Here, we designed and characterized four categories of engineered nanoparticle immunogens that recapitulate the structural and antigenic properties of prefusion Spike (S), S1 and RBD. These immunogens induced robust S-binding, ACE2-inhibition, and authentic and pseudovirus neutralizing antibodies against SARS-CoV-2 in mice. A Spike-ferritin nanoparticle (SpFN) vaccine elicited neutralizing titers more than 20-fold higher than convalescent donor serum, following a single immunization, while RBD-Ferritin nanoparticle (RFN) immunogens elicited similar responses after two immunizations. Passive transfer of IgG purified from SpFN- or RFN-immunized mice protected K18-hACE2 transgenic mice from a lethal SARS-CoV-2 virus challenge. Furthermore, SpFN- and RFN-immunization elicited ACE2 blocking activity and neutralizing ID50 antibody titers >2,000 against SARS-CoV-1, along with high magnitude neutralizing titers against major VoC. These results provide design strategies for pan-coronavirus vaccine development. HIGHLIGHTS: Iterative structure-based design of four Spike-domain Ferritin nanoparticle classes of immunogensSpFN-ALFQ and RFN-ALFQ immunization elicits potent neutralizing activity against SARS-CoV- ...HIGHLIGHTS: Iterative structure-based design of four Spike-domain Ferritin nanoparticle classes of immunogensSpFN-ALFQ and RFN-ALFQ immunization elicits potent neutralizing activity against SARS-CoV-2, variants of concern, and SARS-CoV-1Passively transferred IgG from immunized C57BL/6 mice protects K18-hACE2 mice from lethal SARS-CoV-2 challenge.
History
Deposition
Nov 19, 2021
-
Header (metadata) release
Dec 22, 2021
-
Map release
Dec 22, 2021
-
Update
Jan 17, 2024
-
Current status
Jan 17, 2024
Processing site: RCSB / Status: Released
-
Structure visualization
Movie
Surface view with section colored by density value
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)
EMDB accession codes are about to change! (news from PDBe EMDB page)
The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
The EM Navigator/Yorodumi systems omit the EMD- prefix.
Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator
Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.
Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi