Journal: Science / Year: 2014 Title: Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures. Authors: Nicholas J Shikuma / Martin Pilhofer / Gregor L Weiss / Michael G Hadfield / Grant J Jensen / Dianne K Newman / Abstract: Many benthic marine animal populations are established and maintained by free-swimming larvae that recognize cues from surface-bound bacteria to settle and metamorphose. Larvae of the tubeworm ...Many benthic marine animal populations are established and maintained by free-swimming larvae that recognize cues from surface-bound bacteria to settle and metamorphose. Larvae of the tubeworm Hydroides elegans, an important biofouling agent, require contact with surface-bound bacteria to undergo metamorphosis; however, the mechanisms that underpin this microbially mediated developmental transition have been enigmatic. Here, we show that a marine bacterium, Pseudoalteromonas luteoviolacea, produces arrays of phage tail-like structures that trigger metamorphosis of H. elegans. These arrays comprise about 100 contractile structures with outward-facing baseplates, linked by tail fibers and a dynamic hexagonal net. Not only do these arrays suggest a novel form of bacterium-animal interaction, they provide an entry point to understanding how marine biofilms can trigger animal development.
History
Deposition
Dec 31, 2013
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Header (metadata) release
Jan 15, 2014
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Map release
Feb 12, 2014
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Update
Feb 12, 2014
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Current status
Feb 12, 2014
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_2544.map.gz / Format: CCP4 / Size: 1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Subtomogram average of a contracted contractile structure in a MAC array
Voxel size
X=Y=Z: 16.8 Å
Density
Contour Level
By AUTHOR: 127.0 / Movie #1: 127
Minimum - Maximum
22.141006470000001 - 226.5
Average (Standard dev.)
115.221542360000001 (±11.83436584)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
65
65
65
Spacing
65
65
65
Cell
A=B=C: 1092.0 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
16.8
16.8
16.8
M x/y/z
65
65
65
origin x/y/z
0.000
0.000
0.000
length x/y/z
1092.000
1092.000
1092.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-207
-207
-206
NX/NY/NZ
414
414
414
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
65
65
65
D min/max/mean
22.141
226.500
115.222
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Supplemental data
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Sample components
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Entire : Culture of Pseudoalteromonas violacea cells
Entire
Name: Culture of Pseudoalteromonas violacea cells
Components
Sample: Culture of Pseudoalteromonas violacea cells
Organelle or cellular component: Contracted contractile structure in a MAC array
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Supramolecule #1000: Culture of Pseudoalteromonas violacea cells
Supramolecule
Name: Culture of Pseudoalteromonas violacea cells / type: sample / ID: 1000 / Number unique components: 1
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Supramolecule #1: Contracted contractile structure in a MAC array
Supramolecule
Name: Contracted contractile structure in a MAC array / type: organelle_or_cellular_component / ID: 1 / Oligomeric state: Structure is part of a MAC array / Recombinant expression: No / Database: NCBI
Source (natural)
Organism: Pseudoalteromonas luteoviolacea (bacteria) / Organelle: MAC array / Location in cell: cytoplasmic and extracellular
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Experimental details
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Structure determination
Method
cryo EM
Processing
subtomogram averaging
Aggregation state
cell
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Sample preparation
Grid
Details: Quantifoil
Vitrification
Cryogen name: ETHANE-PROPANE MIXTURE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK III
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Electron microscopy
Microscope
FEI POLARA 300
Specialist optics
Energy filter - Name: GIF
Date
Jul 12, 2013
Image recording
Category: CCD / Film or detector model: GATAN K2 (4k x 4k)
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Sample stage
Specimen holder model: OTHER
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
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Image processing
Details
PEET
Final reconstruction
Algorithm: OTHER / Software - Name: ETOMO / Number subtomograms used: 25
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