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- EMDB-25100: Unmethylated Mtb Ribosome 50S with SEQ-9 -

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Basic information

Entry
Database: EMDB / ID: EMD-25100
TitleUnmethylated Mtb Ribosome 50S with SEQ-9
Map data
Sample
  • Complex: Unmethylated Mth Ribosome with SEQ-9
    • Protein or peptide: x 48 types
    • RNA: x 3 types
  • Ligand: x 3 types
Keywordsunmethylated / Mtb ribosome / drug discovery / SEQ-9 / Structural Genomics / TB Structural Genomics Consortium / TBSGC / RIBOSOME-ANTIBIOTIC complex
Function / homology
Function and homology information


large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit ...large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / zinc ion binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 ...Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein S14/S29 / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S19, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S13, bacterial-type / Ribosomal protein L27 signature. / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / : / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S15, bacterial-type / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL14 ...Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS8 / 50S ribosomal protein L15 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL13 / 30S ribosomal protein THX / 50S ribosomal protein L36 / 50S ribosomal protein L34 / : / Small ribosomal subunit protein uS12
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsXing Z / Cui Z
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P01AI095208 United States
Welch FoundationA1863 United States
CitationJournal: Cell / Year: 2023
Title: Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents.
Authors: Jidong Zhang / Christine Lair / Christine Roubert / Kwame Amaning / María Belén Barrio / Yannick Benedetti / Zhicheng Cui / Zhongliang Xing / Xiaojun Li / Scott G Franzblau / Nicolas ...Authors: Jidong Zhang / Christine Lair / Christine Roubert / Kwame Amaning / María Belén Barrio / Yannick Benedetti / Zhicheng Cui / Zhongliang Xing / Xiaojun Li / Scott G Franzblau / Nicolas Baurin / Florence Bordon-Pallier / Cathy Cantalloube / Stephanie Sans / Sandra Silve / Isabelle Blanc / Laurent Fraisse / Alexey Rak / Lasse B Jenner / Gulnara Yusupova / Marat Yusupov / Junjie Zhang / Takushi Kaneko / T J Yang / Nader Fotouhi / Eric Nuermberger / Sandeep Tyagi / Fabrice Betoudji / Anna Upton / James C Sacchettini / Sophie Lagrange /
Abstract: The emergence of drug-resistant tuberculosis has created an urgent need for new anti-tubercular agents. Here, we report the discovery of a series of macrolides called sequanamycins with outstanding ...The emergence of drug-resistant tuberculosis has created an urgent need for new anti-tubercular agents. Here, we report the discovery of a series of macrolides called sequanamycins with outstanding in vitro and in vivo activity against Mycobacterium tuberculosis (Mtb). Sequanamycins are bacterial ribosome inhibitors that interact with the ribosome in a similar manner to classic macrolides like erythromycin and clarithromycin, but with binding characteristics that allow them to overcome the inherent macrolide resistance of Mtb. Structures of the ribosome with bound inhibitors were used to optimize sequanamycin to produce the advanced lead compound SEQ-9. SEQ-9 was efficacious in mouse models of acute and chronic TB as a single agent, and it demonstrated bactericidal activity in a murine TB infection model in combination with other TB drugs. These results support further investigation of this series as TB clinical candidates, with the potential for use in new regimens against drug-susceptible and drug-resistant TB.
History
DepositionOct 4, 2021-
Header (metadata) releaseOct 12, 2022-
Map releaseOct 12, 2022-
UpdateJun 5, 2024-
Current statusJun 5, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_25100.png

Downloads & links

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Map

FileDownload / File: emd_25100.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.83 Å
Density
Contour LevelBy AUTHOR: 0.4
Minimum - Maximum-0.3354427 - 1.6184096
Average (Standard dev.)0.017556854 (±0.08232476)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 348.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_25100_msk_1.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: #2

Fileemd_25100_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_25100_half_map_2.map
Projections & Slices
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Sample components

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Entire : Unmethylated Mth Ribosome with SEQ-9

EntireName: Unmethylated Mth Ribosome with SEQ-9
Components
  • Complex: Unmethylated Mth Ribosome with SEQ-9
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31
    • RNA: 23S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: peptide
  • Ligand: ZINC ION
  • Ligand: Sequanamycin 9
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Unmethylated Mth Ribosome with SEQ-9

SupramoleculeName: Unmethylated Mth Ribosome with SEQ-9 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)

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Macromolecule #1: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 6.525757 KDa
SequenceString:
MAVPKRRKSR SNTRSRRSQW KAAKTELVGV TVAGHAHKVP RRLLKAARLG LIDFDKR

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #2: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 6.512581 KDa
SequenceString:
MASSTDVRPK ITLACEVCKH RNYITKKNRR NDPDRLELKK FCPNCGKHQA HRETR

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #3: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 5.643697 KDa
SequenceString:
MTKGKRTFQP NNRRRARVHG FRLRMRTRAG RSIVSSRRRK GRRTLSA

UniProtKB: 50S ribosomal protein L34

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Macromolecule #4: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 7.24143 KDa
SequenceString:
MPKAKTHSGA SKRFRRTGTG KIVRQKANRR HLLEHKPSTR TRRLDGRTVV AANDTKRVTS LLNG

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #5: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 4.323239 KDa
SequenceString:
VKVNPSVKPI CDKCRLIRRH GRVMVICSDP RHKQRQG

UniProtKB: 50S ribosomal protein L36

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Macromolecule #6: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 8.770074 KDa
SequenceString:
MKSDIHPAYE ETTVVCGCGN TFQTRSTKPG GRIVVEVCSQ CHPFYTGKQK ILDSGGRVAR FEKRYGKRKV GADKAVSTGK

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #9: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 30.512807 KDa
SequenceString: AIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGRG GRNAHGRITT RHKGGGHKRA YRMIDFRRND KDGVNAKVAH IEYDPNRTA RIALLHYLDG EKRYIIAPNG LSQGDVVESG ANADIKPGNN LPLRNIPAGT LIHAVELRPG GGAKLARSAG S SIQLLGKE ...String:
AIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGRG GRNAHGRITT RHKGGGHKRA YRMIDFRRND KDGVNAKVAH IEYDPNRTA RIALLHYLDG EKRYIIAPNG LSQGDVVESG ANADIKPGNN LPLRNIPAGT LIHAVELRPG GGAKLARSAG S SIQLLGKE ASYASLRMPS GEIRRVDVRC RATVGEVGNA EQANINWGKA GRMRWKGKRP SVRGVVMNPV DHPHGGGEGK TS GGRHPVS PWGKPEGRTR NANKSSNKFI VRRRRTGKKH SR

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #10: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 22.680992 KDa
SequenceString: ARKGILGTKL GMTQVFDESN RVVPVTVVKA GPNVVTRIRT PERDGYSAVQ LAYGEISPRK VNKPLTGQYT AAGVNPRRYL AELRLDDSD AATEYQVGQE LTAEIFADGS YVDVTGTSKG KGFAGTMKRH GFRGQGASHG AQAVHRRPGS IGGCATPARV F KGTRMAGR ...String:
ARKGILGTKL GMTQVFDESN RVVPVTVVKA GPNVVTRIRT PERDGYSAVQ LAYGEISPRK VNKPLTGQYT AAGVNPRRYL AELRLDDSD AATEYQVGQE LTAEIFADGS YVDVTGTSKG KGFAGTMKRH GFRGQGASHG AQAVHRRPGS IGGCATPARV F KGTRMAGR MGNDRVTVLN LLVHKVDAEN GVLLIKGAVP GRTGGLVMVR SAIKR

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #11: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 22.086125 KDa
SequenceString: LKIDVKTPAG KVDGAIELPA ELFDVPANIA LMHQVVTAQR AAARQGTHST KTRGEVSGGG RKPYRQKGTG RARQGSTRAP QFTGGGVVH GPKPRDYSQR TPKKMIAAAL RGALSDRARN GRIHAITELV EGQNPSTKSA RAFLASLTER KQVLVVIGRS D EAGAKSVR ...String:
LKIDVKTPAG KVDGAIELPA ELFDVPANIA LMHQVVTAQR AAARQGTHST KTRGEVSGGG RKPYRQKGTG RARQGSTRAP QFTGGGVVH GPKPRDYSQR TPKKMIAAAL RGALSDRARN GRIHAITELV EGQNPSTKSA RAFLASLTER KQVLVVIGRS D EAGAKSVR NLPGVHILAP DQLNTYDVLR ADDVVFSVEA LNAYIAANT

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #12: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 20.048164 KDa
SequenceString:
QPRLKERYRS EIRDALRKQF GYGNVMQIPT VTKVVVNMGV GEAARDAKLI NGAVNDLALI TGQKPEVRRA RKSIAQFKLR EGMPVGVRV TLRGDRMWEF LDRLTSIALP RIRDFRGLSP KQFDGVGNYT FGLAEQAVFH EVDVDKIDRV RGMDINVVTS A ATDDEGRA LLRALGFPFK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #13: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 19.190088 KDa
SequenceString:
RIGKQPIPVP AGVDVTIEGQ SISVKGPKGT LGLTVAEPIK VARNDDGAIV VTRPDDERRN RSLHGLSRTL VSNLVTGVTQ GYTTKMEIF GVGYRVQLKG SNLEFALGYS HPVVIEAPEG ITFAVQAPTK FTVSGIDKQK VGQIAANIRR LRRPDPYKGK G VRYEGEQI RRKVGKTGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #14: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 16.196654 KDa
SequenceString:
MKLILTADVD HLGSIGDTVE VKDGYGRNFL LPRGLAIVAS RGAQKQADEI RRARETKSVR DLEHANEIKA AIEALGPIAL PVKTSADSG KLFGSVTAAD VVAAIKKAGG PNLDKRIVRL PKTHIKAVGT HFVSVHLHPE IDVEVSLDVV AQS

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #15: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 21.646121 KDa
SequenceString: MLVGKLLGWR AVRRDLGSMS VAETNPPFTP DRRPGHPGIH PREKKVSAVP TYAPKAGDTT RSWYVIDATD VVLGRLAVAA ANLLRGKHK PTFAPNVDGG DFVIVINADK VAISGDKLQH KMVYRHSGYP GGLHKRTIGE LMQRHPDRVV EKAILGMLPK N RLSRQIQR ...String:
MLVGKLLGWR AVRRDLGSMS VAETNPPFTP DRRPGHPGIH PREKKVSAVP TYAPKAGDTT RSWYVIDATD VVLGRLAVAA ANLLRGKHK PTFAPNVDGG DFVIVINADK VAISGDKLQH KMVYRHSGYP GGLHKRTIGE LMQRHPDRVV EKAILGMLPK N RLSRQIQR KLRVYAGPEH PHSAQQPVPY ELKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #16: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 13.419649 KDa
SequenceString:
VIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RPDGSYIKFD ENAAVIIKP DNDPRGTRIF GPVGRELREK RFMKIISLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #17: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 15.566126 KDa
SequenceString:
MTLKLHDLRP ARGSKIARTR VGRGDGSKGK TAGRGTKGTR ARKQVPVTFE GGQMPIHMRL PKLKGFRNRF RTEYEIVNVG DINRLFPQG GAVGVDDLVA KGAVRKNALV KVLGDGKLTA KVDVSAHKFS GSARAKITAA GGSATEL

UniProtKB: 50S ribosomal protein L15

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Macromolecule #18: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 15.724277 KDa
SequenceString:
MLIPRKVKHR KQHHPRQRGI ASGGTTVNFG DYGIQALEHA YVTNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP NEGVARAALT RAIHKLPIKA RIITREEQF

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #19: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 19.512273 KDa
SequenceString:
MPKPTKGPRL GGSSSHQKAI LANLATSLFE HGRITTTEPK ARALRPYAEK LITHAKKGAL HNRREVLKKL RDKDVVHTLF AEIGPFFAD RDGGYTRIIK IEARKGDNAP MAVIELVREK TVTSEANRAR RVAAAQAKAK KAAAMPTEES EAKPAEEGDV V GASEPDAK APEEPPAEAP EN

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #20: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 13.211122 KDa
SequenceString:
MAQSVSATRR ISRLRRHTRL RKKLSGTAER PRLVVHRSAR HIHVQLVNDL NGTTVAAASS IEADVRGVPG DKKARSVRVG QLIAERAKA AGIDTVVFDR GGYTYGGRIA ALADAARENG LSF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #21: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 13.041126 KDa
SequenceString:
MNRLDFVDKP SLRDDIPAFN PGDTINVHVK VIEGAKERLQ VFKGVVIRRQ GGGIRETFTV RKESYGVGVE RTFPVHSPNI DHIEVVTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #22: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 14.557777 KDa
SequenceString:
MARVKRAVNA HKKRRSILKA SRGYRGQRSR LYRKAKEQQL HSLNYAYRDR RARKGEFRKL WIARINAAAR LNDITYNRLI QGLKAAGVE VDRKNLADIA ISDPAAFTAL VDVARAALPE DVNAPSGEAA

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #23: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 11.173147 KDa
SequenceString:
MMATYAIVKT GGKQYKVAVG DVVKVEKLES EQGEKVSLPV ALVVDGATVT TDAKALAKVA VTGEVLGHTK GPKIRIHKFK NKTGYHKRQ GHRQQLTVLK VTGIA

UniProtKB: UNIPROTKB: A0A7G7DRC6

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Macromolecule #24: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 20.424594 KDa
SequenceString: MTAATKATEY PSAVAKARFV RVSPRKARRV IDLVRGRSVS DALDILRWAP QAASGPVAKV IASAAANAQN NGGLDPATLV VATVYADQG PTAKRIRPRA QGRAFRIRRR TSHITVVVES RPAKDQRSAK SSRARRTEAS KAASKVGATA PAKKAAAKAP A KKAPASSG ...String:
MTAATKATEY PSAVAKARFV RVSPRKARRV IDLVRGRSVS DALDILRWAP QAASGPVAKV IASAAANAQN NGGLDPATLV VATVYADQG PTAKRIRPRA QGRAFRIRRR TSHITVVVES RPAKDQRSAK SSRARRTEAS KAASKVGATA PAKKAAAKAP A KKAPASSG VKKTPAKKAP AKKAPAKASE TSAAKGGSD

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #25: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 10.976785 KDa
SequenceString:
MATLADPRDI ILAPVISEKS YGLLDDNVYT FLVRPDSNKT QIKIAVEKIF AVKVASVNTA NRQGKRKRTR TGYGKRKSTK RAIVTLAPG SRPIDLFGAP A

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #26: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 11.500274 KDa
SequenceString:
MKVHKGDTVL VISGKDKGAK GKVLQAYPDR NRVLVEGVNR IKKHTAISTT QRGARSGGIV TQEAPIHVSN VMVVDSDGKP TRIGYRVDE ETGKRVRISK RNGKDI

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #27: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 22.466166 KDa
SequenceString: MAKSASNQLR VTVRTETGKG ASRRARRAGK IPAVLYGHGA EPQHLELPGH DYAAVLRHSG TNAVLTLDIA GKEQLALTKA LHIHPIRRT IQHADLLVVR RGEKVVVEVS VVVEGQAGPD TLVTQETNSI EIEAEALSIP EQLTVSIEGA EPGTQLTAGQ I ALPAGVSL ...String:
MAKSASNQLR VTVRTETGKG ASRRARRAGK IPAVLYGHGA EPQHLELPGH DYAAVLRHSG TNAVLTLDIA GKEQLALTKA LHIHPIRRT IQHADLLVVR RGEKVVVEVS VVVEGQAGPD TLVTQETNSI EIEAEALSIP EQLTVSIEGA EPGTQLTAGQ I ALPAGVSL ISDPDLLVVN VVKAPTAEEL EGEVAGAEEA EEAAVEAGEA EAAGESE

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #28: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 8.987272 KDa
SequenceString:
MAHKKGASSS RNGRDSAAQR LGVKRYGGQV VKAGEILVRQ RGTKFHPGVN VGRGGDDTLF AKTAGAVEFG IKRGRKTVSI VGSTTA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #29: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 6.920075 KDa
SequenceString:
MAAVCDICGK GPGFGKSVSH SHRRTSRRWD PNIQTVHAVT RPGGNKKRLN VCTSCIKAGK ITRG

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #30: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 8.872015 KDa
SequenceString:
MAVGVSPGEL RELTDEELAE RLRESKEELF NLRFQMATGQ LNNNRRLRTV RQEIARIYTV LRERELGLAT GPDGKES

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #31: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 7.361569 KDa
SequenceString:
MSQLKITQVR STIGARWKQR ESLRTLGLRR IRHSVIREDN AATRGLIAVV RHLVEVEPAQ TGGKT

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #33: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 30.071018 KDa
SequenceString: MGQKINPHGF RLGITTDWKS RWYADKQYAE YVKEDVAIRR LLSSGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY ...String:
MGQKINPHGF RLGITTDWKS RWYADKQYAE YVKEDVAIRR LLSSGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY REGRVPLHTL RADIDYGLYE AKTTFGRIGV KVWIYKGDIV GGKRELAAAA PAGADRPRRE RPSGTRPRRS GA SGTTATG TDAGRAAGGE EAAPDAAAPV EAQSTES

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #34: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 23.515898 KDa
SequenceString: MARYTGPVTR KSRRLRTDLV GGDQAFEKRP YPPGQHGRAR IKESEYLLQL QEKQKARFTY GVMEKQFRRY YEEAVRQPGK TGEELLKIL ESRLDNVIYR AGLARTRRMA RQLVSHGHFN VNGVHVNVPS YRVSQYDIVD VRDKSLNTVP FQIARETAGE R PIPSWLQV ...String:
MARYTGPVTR KSRRLRTDLV GGDQAFEKRP YPPGQHGRAR IKESEYLLQL QEKQKARFTY GVMEKQFRRY YEEAVRQPGK TGEELLKIL ESRLDNVIYR AGLARTRRMA RQLVSHGHFN VNGVHVNVPS YRVSQYDIVD VRDKSLNTVP FQIARETAGE R PIPSWLQV VGERQRVLIH QLPERAQIDV PLTEQLIVEY YSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #35: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 22.925242 KDa
SequenceString: MAEQPAGQAG TTDNRDARGD REGRRRDSGR GSRERDGEKS NYLERVVAIN RVSKVVKGGR RFSFTALVIV GDGNGMVGVG YGKAKEVPA AIAKGVEEAR KSFFRVPLIG GTITHPVQGE AAAGVVLLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL G SDNAINVV ...String:
MAEQPAGQAG TTDNRDARGD REGRRRDSGR GSRERDGEKS NYLERVVAIN RVSKVVKGGR RFSFTALVIV GDGNGMVGVG YGKAKEVPA AIAKGVEEAR KSFFRVPLIG GTITHPVQGE AAAGVVLLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL G SDNAINVV HATVAALKLL QRPEEVAARR GLPIEDVAPA GMLKARRKSE ALAASVLPDR TI

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #36: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 10.952728 KDa
SequenceString:
MRPYEIMVIL DPTLDERTVA PSLETFLNVV RKDGGKVEKV DIWGKRRLAY EIAKHAEGIY VVIDVKAAPA TVSELDRQLS LNESVLRTK VMRTDKH

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #37: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 17.633418 KDa
SequenceString:
MPRKGPAPKR PLVNDPVYGS QLVTQLVNKV LLKGKKSLAE RIVYGALEQA RDKTGTDPVI TLKRALDNVK PALEVRSRRV GGATYQVPV EVRPDRSTTL ALRWLVGYSR QRREKTMIER LANEILDASN GLGASVKRRE DTHKMAEANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #38: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 14.432563 KDa
SequenceString:
MTMTDPIADF LTRLRNANSA YHDEVSLPHS KLKANIAQIL KNEGYISDFR TEDARVGKSL VIQLKYGPSR ERSIAGLRRV SKPGLRVYA KSTNLPRVLG GLGVAIISTS SGLLTDRQAA RQGVGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #39: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 16.465061 KDa
SequenceString:
MTETTPAPQT PAAPAGPAQS FVLERPIQTV GRRKEAVVRV RLVPGTGKFD LNGRSLEDYF PNKVHQQLIK APLVTVDRVE SFDIFAHLG GGGPSGQAGA LRLGIARALI LVSPEDRPAL KKAGFLTRDP RATERKKYGL KKARKAPQYS KR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #40: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 11.45234 KDa
SequenceString:
MAGQKIRIRL KAYDHEAIDA SARKIVETVV RTGASVVGPV PLPTEKNVYC VIRSPHKYKD SREHFEMRTH KRLIDIIDPT PKTVDALMR IDLPASVDVN IQ

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #41: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 14.815918 KDa
SequenceString:
MPPAKKGPAT SARKGQKTRR REKKNVPHGA AHIKSTFNNT IVTITDPQGN VIAWASSGHV GFKGSRKSTP FAAQLAAENA ARKAQDHGV RKVDVFVKGP GSGRETAIRS LQAAGLEVGA ISDVTPQPHN GVRPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #42: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 13.882341 KDa
SequenceString:
MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKD LPGVRYKIIR GSLDTQGVKN RKQARSRYGA KKEKG

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #43: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 14.383779 KDa
SequenceString:
MARLVGVDLP RDKRMEVALT YIFGIGRTRS NEILAATGID RDLRTRDLTE EQLIHLRDYI EANLKVEGDL RREVQADIRR KIEIGCYQG LRHRRGMPVR GQRTKTNART RKGPKRTIAG KKKAR

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #44: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 6.841249 KDa
SequenceString:
MAKKALVNKA AGKPRFAVRA YTRCSKCGRP RAVYRKFGLC RICLREMAHA GELPGVQKSS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #45: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 10.500277 KDa
SequenceString:
MALTAEQKKE ILRSYGLHET DTGSPEAQIA LLTKRIADLT EHLKVHKHDH HSRRGLLLLV GRRRRLIKYI SQIDVERYRS LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #46: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 17.4661 KDa
SequenceString:
MAVKIKLTRL GKIRNPQYRV AVADARTRRD GRAIEVIGRY HPKEEPSLIE INSERAQYWL SVGAQPTEPV LKLLKITGDW QKFKGLPGA QGRLKVAAPK PSKLEVFNAA LAAADGGPTT EATKPKKKSP AKKAAKAAEP APQPEQPDTP ALGGEQAELT A ES

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #47: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 14.776373 KDa
SequenceString:
MAEAKTGAKA APRVAKAAKA APKKAAPNDA EAIGAANAAN VKGPKHTPRT PKPRGRRKTR IGYVVSDKMQ KTIVVELEDR MRHPLYGKI IRTTKKVKAH DEDSVAGIGD RVSLMETRPL SATKRWRLVE ILEKAK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #48: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 9.565279 KDa
SequenceString:
MAKSSKRRPA PEKPVKTRKC VFCAKKDQAI DYKDTALLRT YISERGKIRA RRVTGNCVQH QRDIALAVKN AREVALLPFT SSVR

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #49: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 10.830629 KDa
SequenceString:
MPRSLKKGPF VDEHLLKKVD VQNEKNTKQV IKTWSRRSTI IPDFIGHTFA VHDGRKHVPV FVTESMVGHK LGEFAPTRTF KGHIKDDRK SKRR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #50: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 9.428941 KDa
SequenceString:
MANIKSQQKR NRTNERARLR NKAVKSSLRT AVRAFREAAH AGDKAKAAEL LASTNRKLDK AASKGVIHKN QAANKKSALA QALNKL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #51: peptide

MacromoleculeName: peptide / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 2.664129 KDa
SequenceString:
AKRGRKKRDR KYSKANHGKR PN

UniProtKB: 30S ribosomal protein THX

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Macromolecule #7: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 1.017862688 MDa
SequenceString: UUGUAAGUGU CUAAGGGCGC AUGGUGGAUG CCUUGGCAUC GAGAGCCGAU GAAGGACGUG GGAGGCUGCG AUAUGCCUCG GGGAGCUGU CAACCGAGCG UGGAUCCGAG GAUUUCCGAA UGGGGAAACC CAGCACGAGU GAUGUCGUGC UACCCGCAUC U GAAUAUAU ...String:
UUGUAAGUGU CUAAGGGCGC AUGGUGGAUG CCUUGGCAUC GAGAGCCGAU GAAGGACGUG GGAGGCUGCG AUAUGCCUCG GGGAGCUGU CAACCGAGCG UGGAUCCGAG GAUUUCCGAA UGGGGAAACC CAGCACGAGU GAUGUCGUGC UACCCGCAUC U GAAUAUAU AGGGUGCGGG AGGGAACGCG GGGAAGUGAA ACAUCUCAGU ACCCGUAGGA GGAGAAAACA AUUGUGAUUC CG CAAGUAG UGGCGAGCGA ACGCGGAACA GGCUAAACCG CACGCAUGGG UAACCGGGUA GGGGUUGUGU GUGCGGGGUU GUG GGAGGA UAUGUCUCAG CGCUACCCGG CUGAGAGGCA GUCAGAAAGU GUCGUGGUUA GCGGAAGUGG CCUGGGAUGG UCUG CCGUA GACGGUGAGA GCCCGGUACG CGAAAACCCG GCACCUGCCU AGUAUCAAUU CCCGAGUAGC AGCGGGCCCG UGGAA UCCG CUGUGAAUCC GCCGGGACCA CCCGGUAAGC CUAAAUACUC CUCGAUGACC GAUAGCGGAU UAGUACCGUG AGGGAA UGG UGAAAAGUAC CCCGGGAGGG GAGUGAAAGA GUACCUGAAA CCGUGUGCCU ACAAUCCGUC AGAGCCUCCU UUUCCUC UC CGGAGGAGGG UGGUGAUGGC GUGCCUUUUG AAGAAUGAGC CUGCGAGUCA GGGACAUGUC GCAAGGUUAA CCCGUGUG G GGUAGCCGCA GCGAAAGCGA GUCUGAAUAG GGCGACCCAC ACGCGCAUAC GCGCGUGUGA AUAGUGGCGU GUUCUGGAC CCGAAGCGGA GUGAUCUACC CAUGGCCAGG GUGAAGCGCG GGUAAGACCG CGUGGAGGCC CGAACCCACU UAGGUUGAAG ACUGAGGGG AUGAGCUGUG GGUAGGGGUG AAAGGCCAAU CAAACUCCGU GAUAGCUGGU UCUCCCCGAA AUGCAUUUAG G UGCAGCGU UGCGUGGUUC ACCGCGGAGG UAGAGCUACU GGAUGGCCGA UGGGCCCUAC UAGGUUACUG ACGUCAGCCA AA CUCCGAA UGCCGUGGUG UAAAGCGUGG CAGUGAGACG GCGGGGGAUA AGCUCCGUAC GUCGAAAGGG AAACAGCCCA GAU CGCCGG CUAAGGCCCC CAAGCGUGUG CUAAGUGGGA AAGGAUGUGC AGUCGCAAAG ACAACCAGGA GGUUGGCUUA GAAG CAGCC ACCCUUGAAA GAGUGCGUAA UAGCUCACUG GUCAAGUGAU UGUGCGCCGA UAAUGUAGCG GGGCUCAAGC ACACC GCCG AAGCCGCGGC ACAUCCACCU UGUGGUGGGU GUGGGUAGGG GAGCGUCCCU CAUUCAGCGA AGCCACCGGG UGACCG GUG GUGGAGGGUG GGGGAGUGAG AAUGCAGGCA UGAGUAGCGA CAAGGCAAGU GAGAACCUUG CCCGCCGAAA GACCAAG GG UUCCUGGGCC AGGCCAGUCC GCCCAGGGUG AGUCGGGACC UAAGGCGAGG CCGACAGGCG UAGUCGAUGG ACAACGGG U UGAUAUUCCC GUACCCGUGU GUGGGCGCCC GUGACGAAUC AGCGGUACUA ACCACCCAAA ACCGGAUCGA UCACUCCCC UUCGGGGGUG UGGAGUUCUG GGGCUGCGUG GGAACUUCGC UGGUAGUAGU CAAGCGAAGG GGUGACGCAG GAAGGUAGCC GUACCAGUC AGUGGUAACA CUGGGGCAAG CCGGUAGGGA GAGCGAUAGG CAAAUCCGUC GCUCACUAAU CCUGAGAGGU G ACGCAUAG CCGGUUGAGG CGAAUUCGGU GAUCCUCUGC UGCCAAGAAA AGCCUCUAGC GAGCACACAC ACGGCCCGUA CC CCAAACC GACACAGGUG GUCAGGUAGA GCAUACCAAG GCGUACGAGA UAACUAUGGU UAAGGAACUC GGCAAAAUGC CCC CGUAAC UUCGGGAGAA GGGGGACCGG AAUAUCGUGA ACACCCUUGC GGUGGGAGCG GGAUCCGGUC GCAGAAACCA GUGA GGAGC GACUGUUUAC UAAAAACACA GGUCCGUGCG AAGUCGCAAG ACGAUGUAUA CGGACUGACG CCUGCCCGGU GCUGG AAGG UUAAGAGGAC CCGUUAACCC GCAAGGGUGA AGCGGAGAAU UUAAGCCCCA GUAAACGGCG GUGGUAACUA UAACCA UCC UAAGGUAGCG AAA(5MU)UCCUUG UCGGGUAAGU UCCGACCUGC ACGAAUGGCG UAACGACUUC UCAACUGUCU CAA CCAUAG ACUCGGCGAA AUUGCACUAC GAGUA(6MZ)AGAU GCUCGUUACG CGCGGCAGGA CGAAAAGACC CCGGGACCUU CACUACAAC UUGGUAUUGA UGUUCGGUAC GGUUUGUGUA GGAUAGGUGG GAGACUGUGA AACCUCGACG CCAGUUGGGG C GGAGUCGU UGUUGAAAUA CCACUCUGAU CGUAUUGGGC AUCUAACCUC GAACCCUGAA UCGGGUUUAG GGACAGUGCC UG GCGGGUA GUUUAACUG(OMG) GGCGGUUGCC UCCUAAAAUG UAACGGAGGC GCCCAAAGGU UCCCUCAACC UGGACGGCA AUCAGGUGGC GAGUGUAAAU GCACAAGGGA GCUUGACUGC GAGACUUACA AGUCAAGCAG GGACGAAAGU CGGGAUUAGU GAUCCGGCA CCCCCGAGUG GAAGGGGUGU CGCUCAACGG AUAAAAGGUA CCCCGGGGAU AACAGGCUGA UCUUCCCCAA G AGUCCAUA UCGACGGGAU GGUUUGGCA(OMC) CUCGAUGUCG GCUCGUCGCA UCCUGGGGCU GGAGCAGGUC CCAAGGGU U GGGCU(OMG)UUCG CCCAUUAAAG CGGCACGCGA GCUGGGUUUA GAACGUCGUG AGACAGUUCG GUCUCUAUCC GCCGC GCGC GUCAGAAACU UGAGGAAACC UGUCCCUAGU ACGAGAGGAC CGGGACGGAC GAACCUCUGG UGCACCAGUU GUCCCG CCA GGGGCACCGC UGGAUAGCCA CGUUCGGUCA GGAUAACCGC UGAAAGCAUC UAAGCGGGAA ACCUUCUCCA AGAUCAG GU UUCUCACCCA CUUGGUGGGA UAAGGCCCCC CGCAGAACAC GGGUUCAAUA GGUCAGACCU GGAAGCUCAG UAAUGGGU G UAGGGAACUG GUGCUAACCG GCCGAAAACU UACAACA

GENBANK: GENBANK: CP023578.1

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Macromolecule #8: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 37.097238 KDa
SequenceString:
UUACGGCGGC CACAGCGGCA GGGAAACGCC CGGUCCCAUU CCGAACCCGG AAGCUAAGCC UGCCAGCGCC GAUGAUACUG CCCCUCCGG GUGGAAAAGU AGGACACCGC CGAACA

GENBANK: GENBANK: CP023593.1

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Macromolecule #32: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Molecular weightTheoretical: 498.41575 KDa
SequenceString: UUUUGUUUGG AGAGUUUGAU CCUGGCUCAG GACGAACGCU GGCGGCGUGC UUAACACAUG CAAGUCGAAC GGAAAGGUCU CUUCGGAGA UACUCGAGUG GCGAACGGGU GAGUAACACG UGGGUGAUCU GCCCUGCACU UCGGGAUAAG CCUGGGAAAC U GGGUCUAA ...String:
UUUUGUUUGG AGAGUUUGAU CCUGGCUCAG GACGAACGCU GGCGGCGUGC UUAACACAUG CAAGUCGAAC GGAAAGGUCU CUUCGGAGA UACUCGAGUG GCGAACGGGU GAGUAACACG UGGGUGAUCU GCCCUGCACU UCGGGAUAAG CCUGGGAAAC U GGGUCUAA UACCGGAUAG GACCACGGGA UGCAUGUCUU GUGGUGGAAA GCGCUUUAGC GGUGUGGGAU GAGCCCGCGG CC UAUCAGC UUGUUGGUGG GGUGACGGCC UACCAAGGCG ACGACGGGUA GCCGGCCUGA GAGGGUGUCC GGCCACACUG GGA CUGAGA UACGGCCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGCACAAUGG GCGCAAGCCU GAUGCAGCGA CGCC GCGUG GGGGAUGACG GCCUUCGGGU UGUAAACCUC UUUCACCAUC GACGAAGGUC CGGGUUCUCU CGGAUUGACG GUAGG UGGA GAAGAAGCAC CGGCCAACUA CGUGCCAGCA GCC(G7M)CGGUAA UACGUAGGGU GCGAGCGUUG UCCGGAAUUA CU GGGCGUA AAGAGCUCGU AGGUGGUUUG UCGCGUUGUU CGUGAAAUCU CACGGCUUAA CUGUGAGCGU GCGGGCGAUA CGG GCAGAC UAGAGUACUG CAGGGGAGAC UGGAAUUCCU GGUGUAGCGG UGGAAUGCGC AGAUAUCAGG AGGAACACCG GUGG CGAAG GCGGGUCUCU GGGCAGUAAC UGACGCUGAG GAGCGAAAGC GUGGGGAGCG AACAGGAUUA GAUACCCUGG UAGUC CACG CCGUAAACGG UGGGUACUAG GUGUGGGUUU CCUUCCUUGG GAUCCGUGCC GUAGCUAACG CAUUAAGUAC CCCGCC UGG GGAGUACGGC CGCAAGGCUA AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGCGGA GCAUGUGGAU UAAUUCG AU (2MG)(5MC)AACGCGAA GAACCUUACC UGGGUUUGAC AUGCACAGGA CGCGUCUAGA GAUAGGCGUU CCCUUGUGGC CUGUGUGCA GGUGGUGCAU GGCUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGCAACCCUU G UCUCAUGU UGCCAGCACG UAAUGGUGGG GACUCGUGAG AGACUGCCGG GGUCAACUCG GAGGAAGGUG GGGAUGACGU CA AGUCAUC AUGCCCCUUA UGUCCAGGGC UUCACACAUG CUACAAUGGC CGGUACAAAG GGCUGCGAUG CCGCGAGGUU AAG CGAAUC CUUAAAAGCC GGUCUCAGUU CGGAUCGGGG UCUGCAACUC GACCCCGUGA AGUCGGAGUC GCUAGUAAUC GCAG AUCAG CAACGCUGCG GUGAAUACGU UCCCGGGCCU UGUACACACC G(4OC)CCGUCACG UCAUGAAAGU CGGUAACACC C GAAGCCAG UGGCCUAACC CUCGGGAGGG AGCUGUCGAA GGUGGGAUCG GCGAUUGGGA CGAAGUCG(UR3)A ACAAGGUA G CCGUACC(2MG)G(MA6) (MA6)GGUGCGGCU GGAUCACCUC CUUUCU

GENBANK: GENBANK: CP020381.2

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Macromolecule #52: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 52 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #53: Sequanamycin 9

MacromoleculeName: Sequanamycin 9 / type: ligand / ID: 53 / Number of copies: 1 / Formula: WDP
Molecular weightTheoretical: 1.243542 KDa
Chemical component information

ChemComp-WDP:
Sequanamycin 9

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Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 447 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
50.0 mMKClPotassium Chloride
10.0 mMNH4ClAmmonium Chloride
10.0 mMMgClMagnesium Chloride
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 4 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK III
DetailsMonodispersed unmethylated Mtb ribosome with SEQ-9

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

7kgb

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 719250
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

7kgb


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7sfr:
Unmethylated Mtb Ribosome 50S with SEQ-9

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