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- EMDB-24868: CryoEM structure of modular PKS holo-Lsd14 stalled at the condens... -

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Basic information

Entry
Database: EMDB / ID: EMD-24868
TitleCryoEM structure of modular PKS holo-Lsd14 stalled at the condensation step and bound to antibody fragment 1B2, composite structure
Map dataComposite map
Sample
  • Complex: Dimeric holo-Lsd14 containing docking domains from DEBS module 3 (holo-Lsd14-DD*) bound to two copies each of Fab 1B2 light and heavy chains and NADP, treated with 2-Acetaminoethyl-thio-3-oxobutanoate
    • Complex: Polyketide synthase
    • Complex: Fab 1B2 heavy chain, Fab 1B2 light chain
    • Protein or peptide: 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion
    • Protein or peptide: Fab 1B2 heavy chain
    • Protein or peptide: Fab 1B2 light chain
  • Ligand: 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE
  • Ligand: 4'-PHOSPHOPANTETHEINE
Function / homology
Function and homology information


6-deoxyerythronolide-B synthase / erythronolide synthase activity / macrolide biosynthetic process / DIM/DIP cell wall layer assembly / fatty acid synthase activity / phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / oxidoreductase activity / plasma membrane / cytoplasm
Similarity search - Function
Erythronolide synthase, docking / Erythronolide synthase, docking domain superfamily / Erythronolide synthase, docking / Polyketide synthase dimerisation element domain / Polyketide synthase dimerisation element domain / Polyketide synthase, docking domain superfmaily / Zinc-binding dehydrogenase / Polyketide synthase, docking domain / Erythronolide synthase docking domain / : ...Erythronolide synthase, docking / Erythronolide synthase, docking domain superfamily / Erythronolide synthase, docking / Polyketide synthase dimerisation element domain / Polyketide synthase dimerisation element domain / Polyketide synthase, docking domain superfmaily / Zinc-binding dehydrogenase / Polyketide synthase, docking domain / Erythronolide synthase docking domain / : / : / Polyketide synthase dehydratase domain / : / Polyketide and metazoan fatty acid synthase dehydratase (PKS/mFAS DH) domain profile. / PKS_PP_betabranch / Polyketide synthase dehydratase N-terminal domain / PKS_DH / Polyketide synthase, dehydratase domain / Polyketide synthase, dehydratase domain superfamily / Polyketide synthase, ketoreductase domain / KR domain / Malonyl-CoA ACP transacylase, ACP-binding / Polyketide synthase, C-terminal extension / Ketoacyl-synthetase C-terminal extension / PKS_KR / Acyl transferase domain superfamily / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase/acyl hydrolase/lysophospholipase / Alcohol dehydrogenase, N-terminal / Alcohol dehydrogenase GroES-like domain / Polyketide synthase, enoylreductase domain / Enoylreductase / Ketosynthase family 3 (KS3) domain profile. / Polyketide synthase, phosphopantetheine-binding domain / Phosphopantetheine attachment site / Beta-ketoacyl synthase / Beta-ketoacyl synthase, active site / Ketosynthase family 3 (KS3) active site signature. / Polyketide synthase, beta-ketoacyl synthase domain / Beta-ketoacyl synthase, N-terminal / Beta-ketoacyl synthase, C-terminal / Beta-ketoacyl synthase, N-terminal domain / Beta-ketoacyl synthase, C-terminal domain / GroES-like superfamily / Thiolase-like / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Putative polyketide synthase / 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4
Similarity search - Component
Biological speciesStreptomyces lasalocidi (bacteria) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsBagde SR / Kim C-Y / Fromme JC
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM136258 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM138990 United States
National Science Foundation (NSF, United States)DMR-1719875 United States
CitationJournal: Science / Year: 2021
Title: Modular polyketide synthase contains two reaction chambers that operate asynchronously.
Authors: Saket R Bagde / Irimpan I Mathews / J Christopher Fromme / Chu-Young Kim /
Abstract: Type I modular polyketide synthases are homodimeric multidomain assembly line enzymes that synthesize a variety of polyketide natural products by performing polyketide chain extension and β-keto ...Type I modular polyketide synthases are homodimeric multidomain assembly line enzymes that synthesize a variety of polyketide natural products by performing polyketide chain extension and β-keto group modification reactions. We determined the 2.4-angstrom-resolution x-ray crystal structure and the 3.1-angstrom-resolution cryo–electron microscopy structure of the Lsd14 polyketide synthase, stalled at the transacylation and condensation steps, respectively. These structures revealed how the constituent domains are positioned relative to each other, how they rearrange depending on the step in the reaction cycle, and the specific interactions formed between the domains. Like the evolutionarily related mammalian fatty acid synthase, Lsd14 contains two reaction chambers, but only one chamber in Lsd14 has the full complement of catalytic domains, indicating that only one chamber produces the polyketide product at any given time.
History
DepositionSep 13, 2021-
Header (metadata) releaseNov 3, 2021-
Map releaseNov 3, 2021-
UpdateNov 17, 2021-
Current statusNov 17, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 9
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 9
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7s6c
  • Surface level: 9
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_24868.png

Downloads & links

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Map

FileDownload / File: emd_24868.map.gz / Format: CCP4 / Size: 184 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map
Voxel sizeX=Y=Z: 1.043 Å
Density
Contour LevelBy AUTHOR: 7.0 / Movie #1: 9
Minimum - Maximum-21.71567 - 47.70866
Average (Standard dev.)-0.0023788104 (±1.126138)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions364364364
Spacing364364364
CellA=B=C: 379.652 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0431.0431.043
M x/y/z364364364
origin x/y/z0.0000.0000.000
length x/y/z379.652379.652379.652
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ364364364
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS364364364
D min/max/mean-21.71647.709-0.002

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Supplemental data

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Sample components

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Entire : Dimeric holo-Lsd14 containing docking domains from DEBS module 3 ...

EntireName: Dimeric holo-Lsd14 containing docking domains from DEBS module 3 (holo-Lsd14-DD*) bound to two copies each of Fab 1B2 light and heavy chains and NADP, treated with 2-Acetaminoethyl-thio-3-oxobutanoate
Components
  • Complex: Dimeric holo-Lsd14 containing docking domains from DEBS module 3 (holo-Lsd14-DD*) bound to two copies each of Fab 1B2 light and heavy chains and NADP, treated with 2-Acetaminoethyl-thio-3-oxobutanoate
    • Complex: Polyketide synthase
    • Complex: Fab 1B2 heavy chain, Fab 1B2 light chain
    • Protein or peptide: 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion
    • Protein or peptide: Fab 1B2 heavy chain
    • Protein or peptide: Fab 1B2 light chain
  • Ligand: 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE
  • Ligand: 4'-PHOSPHOPANTETHEINE

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Supramolecule #1: Dimeric holo-Lsd14 containing docking domains from DEBS module 3 ...

SupramoleculeName: Dimeric holo-Lsd14 containing docking domains from DEBS module 3 (holo-Lsd14-DD*) bound to two copies each of Fab 1B2 light and heavy chains and NADP, treated with 2-Acetaminoethyl-thio-3-oxobutanoate
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3
Molecular weightTheoretical: 450 KDa

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Supramolecule #2: Polyketide synthase

SupramoleculeName: Polyketide synthase / type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Streptomyces lasalocidi (bacteria)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #3: Fab 1B2 heavy chain, Fab 1B2 light chain

SupramoleculeName: Fab 1B2 heavy chain, Fab 1B2 light chain / type: complex / ID: 3 / Parent: 1
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria) / Recombinant strain: BL21(DE3)

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Macromolecule #1: 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Pol...

MacromoleculeName: 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion
type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: 6-deoxyerythronolide-B synthase
Source (natural)Organism: Streptomyces lasalocidi (bacteria)
Molecular weightTheoretical: 174.085734 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MVTDSEKVAE YLRRATLDLR AARQRIRELE SEPIAIIGMA CRLPGGVDSP EGLWELVDSG TDAIAGFPLD RGWDVEGMYD PDAEAPGKT YVKEAGFLYD AGEFDAGFFG ISPREAVSMD PQQRLMLEAS WEAFERAGLD PARQRGTATG VFVGATATGY V SPAAEVPE ...String:
MVTDSEKVAE YLRRATLDLR AARQRIRELE SEPIAIIGMA CRLPGGVDSP EGLWELVDSG TDAIAGFPLD RGWDVEGMYD PDAEAPGKT YVKEAGFLYD AGEFDAGFFG ISPREAVSMD PQQRLMLEAS WEAFERAGLD PARQRGTATG VFVGATATGY V SPAAEVPE GAEGFAITGN MTAVTSGRIS YTLGLQGPAV TIDTACSSSL VALHLACQSL RQGECTTALA GGVTVMPTPT AF TEFSRQR GLAPDGRCKS FAAAADGTNW AEGVAVLVVE RLSDARRNGH RVLAVVRGTA INQDGASNGL SAPNDLAQER VIR SALDNA GLTASDVDAV EAHGTGTTLG DPIEAQALLA AYGHERPAHR PLRVGSLKSN IGHAGPAAGV AGVIKMVMAM RHGV LPRSL HIDEPTPQVD WSSGAVTLLT EPVDWPDSDR PRRAGVSAFG ISGTNAHVIL EQAPTQDPQQ PAPPVPAAPW LLSAK TPAA LRAQARRLHT HLARHPHPDP TDIAHALATT RTPHEHRAAL VTDDHGTRGP ALAALAEGAP DACLISGTAL SKGRTV FVF PGQGSQWTGM GRELLHTSPE FAAYIAECET ALNDFVDWSL TDVLRGTEGA PGYDRVDVVQ PALFAVMVSL ARLWQHH GI HPDAVIGHSQ GEIAAAHIAG ALSLQDAARI VALRSQALLP LAGLGGMTSL ALPHDQALQL IQPWGQDLSI ASVNGPHS T VVSGTTHALD ELHTTCDTQG VRARRIPVDY ASHSAQVESI RDTVLQAATG INPQPTTIPL YSTVTGQPID GTQLDADYW YTNLRHTVRF EETTRALLGS GHRHFIETTA HPVLALALEE TIEATGSDAR VTGTLRRDHG DLTQLHTALA TAWTHGIDVD WTAVLGDRR TPFELPTYAF QRQRYWLEPG AGVGVPVGGT SAEARFWDAV EDEDLEALVA AIGADGDDAS WAGVLPALAG W RRRQREQS ALDDLRYKVT WKPTAVADGA SATGTWLVVV PESLAGGGWP VVVARAVDQA GGRPVVLSVD AADGADRSRL GL RIHEALG EGPVPDAVVS LLALDPSALP GLPDVPQALA STAALVQALL DLGLEARLWC VTSGAVSVSG ADGPSAPEQA AVW GFGRVA GLEHPHLWAG LVDLPPEADE RTAARLVGVL AGAGGEDQVA LRSSGVFVRR LVRAPASEVP AVRSWKPGGT VLVT GGTGG LGRQVARWLA RGGADHLLLV SRRGVDAPGA DELVDELTDL GARVTVAACD VADRDAVQRL LSEQVPSDAP LTAVI HTAA VLDDGVIDSL SPERMEQVLR VKVGGAVHLY ELTRESDLSA FVLFSSFGST FGLPGLGNYA PGNAALEALA EQWRAE GRP ATAVGWGTWA GGGMADGGVG ERGRTHGIHE LEPALATAAL EQALERDESS PVIIDIDWER FAVAFHAKRP TRGFELV PE AQAALEAADG GPGPDGGAGD PSELLDRLAA LPDAERDRAL LEVVRGNAAS VMSHGAMRTA TLEAVEPTRA FRDLGFDS L MAVELRNRIG AATGLRLAPT LVFDHPTPEA VVRHLRAELG LEGDGAPDPV FDELDGLERA LSSYTPDTDT RVKITKRLE SLLWEWTRSE ADAPDPVDAA DLAAVSDDEM FELIDRELGS ALEHHHHHH

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Macromolecule #2: Fab 1B2 heavy chain

MacromoleculeName: Fab 1B2 heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.447611 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MAEVQLVQSG GGLVQPGRSL RLSCTASGFT FGDYAMSWVR QAPGKGLEWV GFIRSKAYGG TTEYAASVKG RFTISRDDSK SIAYLQMNS LKTEDTAVYY CTRGGTLFDY WGQGTLVTVS SASTKGPSVF PLAPSSKSTS GGTAALGCLV KDYFPEPVTV S WNSGALTS ...String:
MAEVQLVQSG GGLVQPGRSL RLSCTASGFT FGDYAMSWVR QAPGKGLEWV GFIRSKAYGG TTEYAASVKG RFTISRDDSK SIAYLQMNS LKTEDTAVYY CTRGGTLFDY WGQGTLVTVS SASTKGPSVF PLAPSSKSTS GGTAALGCLV KDYFPEPVTV S WNSGALTS GVHTFPAVLQ SSGLYSLSSV VTVPSSSLGT QTYICNVNHK PSNTKVDKKV EPKSCAALVP RGSAHHHHHH AA DYKDDDD KA

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Macromolecule #3: Fab 1B2 light chain

MacromoleculeName: Fab 1B2 light chain / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.715832 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: LFAIPLVVPF YSHSALDVVM TQSPLSLPVT PGEPASISCR SSQSLLHSNG YNYLDWYLQK PGQSPQLLIY LGSNRASGVP DRFSGSGSG TDFTLKISRV EAEDVGVYYC MQSLQTPRLT FGPGTKVDIK RTVAAPSVFI FPPSDEQLKS GTASVVCLLN N FYPRGAKV ...String:
LFAIPLVVPF YSHSALDVVM TQSPLSLPVT PGEPASISCR SSQSLLHSNG YNYLDWYLQK PGQSPQLLIY LGSNRASGVP DRFSGSGSG TDFTLKISRV EAEDVGVYYC MQSLQTPRLT FGPGTKVDIK RTVAAPSVFI FPPSDEQLKS GTASVVCLLN N FYPRGAKV QWKVDNALQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV THQGLSSPVT KSFNRGEC

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Macromolecule #4: 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 1 / Formula: ATR
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATR:
2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE

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Macromolecule #5: 4'-PHOSPHOPANTETHEINE

MacromoleculeName: 4'-PHOSPHOPANTETHEINE / type: ligand / ID: 5 / Number of copies: 1 / Formula: PNS
Molecular weightTheoretical: 358.348 Da
Chemical component information

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4.2 mg/mL
BufferpH: 7.2
Component:
ConcentrationName
100.0 mMsodium chloride
100.0 mMcitrate
10.0 mMHEPES
1.0 mM2-Acetaminoethyl-thio-3-oxobutanoate
0.4 mMmethylmalonyl-CoA
0.8 mMNADP
0.8 mMTCEP
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 120.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details: Blot for 5 seconds before plunging in liquid ethane..
DetailsSize exclusion chromatography purified monodisperse holo-Lsd14-DD* + 1B2 complex was first incubated with KS substrate analog (2-Acetaminoethyl-thio-3-oxobutanoate) and TCEP at 30C for 20 minutes, followed by addition of NADP and methylmalonyl-CoA. The sample was further incubated at 30C for 15 minutes before applying to cryoEM grids.

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 1278 / Average exposure time: 2.2 sec. / Average electron dose: 53.0 e/Å2 / Details: Images were collected as 50 frame movies.
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 79000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 397539
CTF correctionSoftware:
Namedetails
cryoSPARC (ver. 3.2.0)Patch CTF estimation (multi)
RELION (ver. 3.1)
Startup modelType of model: INSILICO MODEL
In silico model: Used Ab-initio Reconstruction job in cryoSPARC to generate starting map.
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1)
Details: Composite map was generated by combining the unsharpened focused maps for KS-KS'-ACP domains (EMD-24863), LDAT domains (EMD-24864), LD'AT' domains (EMD-24865), KR domain (EMD-24866), Fab + ...Details: Composite map was generated by combining the unsharpened focused maps for KS-KS'-ACP domains (EMD-24863), LDAT domains (EMD-24864), LD'AT' domains (EMD-24865), KR domain (EMD-24866), Fab + DD* (EMD-24867), and the unsharpened consensus map (EMD-24862) using Combine Focused Maps in Phenix.
Number images used: 397539
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.2.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)

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Atomic model buiding 1

Initial model
PDB IDChain

7s6b


6c9u

chain_id: H

6c9u

chain_id: L
DetailsModel was manually rebuilt in Coot and phenix.real_space_refine was used for refinement.
RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-7s6c:
CryoEM structure of modular PKS holo-Lsd14 stalled at the condensation step and bound to antibody fragment 1B2, composite structure

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