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- EMDB-24307: Structure of the translating Neurospora crassa ribosome arrested ... -

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Basic information

Entry
Database: EMDB / ID: EMD-24307
TitleStructure of the translating Neurospora crassa ribosome arrested by cycloheximide
Map data
Sample
  • Complex: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
    • Complex: 60S ribosomal subunit
      • RNA: x 3 types
      • Protein or peptide: x 42 types
    • Complex: 40S ribosomal subunit
      • RNA: x 1 types
      • Protein or peptide: x 33 types
    • Complex: tRNATransfer RNA
      • RNA: x 1 types
    • Complex: Nascent peptide
      • Protein or peptide: x 1 types
    • Complex: mRNAMessenger RNA
      • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


positive regulation of translational fidelity / : / preribosome, small subunit precursor / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein kinase A catalytic subunit binding / protein-RNA complex assembly / ribosomal small subunit export from nucleus / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / translational termination ...positive regulation of translational fidelity / : / preribosome, small subunit precursor / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein kinase A catalytic subunit binding / protein-RNA complex assembly / ribosomal small subunit export from nucleus / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / translational termination / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / DNA-(apurinic or apyrimidinic site) endonuclease activity / small-subunit processome / assembly of large subunit precursor of preribosome / protein kinase C binding / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / large ribosomal subunit / ribosome biogenesis / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. ...Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L29e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal L29e protein family / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein L27e superfamily / Ribosomal protein S21e / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein S7e / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal L27e protein family / Ribosomal protein S4, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal
Similarity search - Domain/homology
Uncharacterized protein / 60S ribosomal protein L35 / 40S ribosomal protein S7 / 60S ribosomal protein L5 / 40S ribosomal protein S21 / 60S ribosomal protein L28 / Ubiquitin-40S ribosomal protein S27a / 40S ribosomal protein S26E / 40S ribosomal protein S17 / 60S ribosomal protein L3 ...Uncharacterized protein / 60S ribosomal protein L35 / 40S ribosomal protein S7 / 60S ribosomal protein L5 / 40S ribosomal protein S21 / 60S ribosomal protein L28 / Ubiquitin-40S ribosomal protein S27a / 40S ribosomal protein S26E / 40S ribosomal protein S17 / 60S ribosomal protein L3 / 40S ribosomal protein S0 / Guanine nucleotide-binding protein subunit beta-like protein / Ribosomal protein L26 / 60S ribosomal protein L27 / 40S ribosomal protein S24 / 60S ribosomal protein L22 / 60S ribosomal protein L4-A / 60S ribosomal protein L8 / Cytoplasmic ribosomal protein 10 / 40S ribosomal protein S12 / 40S ribosomal protein S22 / 40S ribosomal protein S5 / 60S ribosomal protein L44 / 60S ribosomal protein L11 / 40S ribosomal protein S27 / 60S ribosomal protein L2 / 60S ribosomal protein L34 / 60s ribosomal protein / 60S ribosomal protein L18 / 40S ribosomal protein S18 / 60S ribosomal protein L32 / 60S ribosomal protein L29 / 60S ribosomal protein L33 / 60S ribosomal protein L43 / 60S ribosomal protein L39 / 40S ribosomal protein S20 / 40S ribosomal protein S2 / Ribosomal protein L19 / 40S ribosomal protein S28 / 60S ribosomal protein L13 / 60S ribosomal protein L10 / 60S ribosomal protein L30 / Ribosomal_L28e domain-containing protein / 60S ribosomal protein L7 / 40S ribosomal protein S19 / 60S ribosomal protein L25 / 40S ribosomal protein S25 / 40S ribosomal protein S13 / 60S ribosomal protein L24 / 40S ribosomal protein S9 / Ribosomal protein Srp1 / 40S ribosomal protein S4 / 60S ribosomal protein L36 / 40S ribosomal protein S16 / 40S ribosomal protein S11 / 60S ribosomal protein L9 / Ribosomal protein S12 / 60S ribosomal protein L6 / 60S ribosomal protein L23 / Ribosomal protein L14 / 40S ribosomal protein S1 / Probable 40s ribosomal protein s10-b / 60S ribosomal protein L15 / 60S ribosomal protein L38 / 40S ribosomal protein S29 / 60S ribosomal protein L17 / 40S ribosomal protein S23 / 60S ribosomal protein L41 / 60S ribosomal protein L16 / 40S ribosomal protein S30 / 60S ribosomal protein L40 / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Ribosomal protein L37 / 60S ribosomal protein L20
Similarity search - Component
Biological speciesNeurospora crassa (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsShen L / Su Z / Yang K / Wu C / Becker T / Bell-Pedersen D / Zhang J / Sachs MS
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R21AI138158 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM126966 United States
Welch FoundationA1863 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: Structure of the translating ribosome arrested by cycloheximide.
Authors: Lunda Shen / Zhaoming Su / Kailu Yang / Cheng Wu / Thomas Becker / Deborah Bell-Pedersen / Junjie Zhang / Matthew S Sachs /
Abstract: Ribosomes translate RNA into proteins. The protein synthesis inhibitor cycloheximide (CHX) is widely used to inhibit eukaryotic ribosomes engaged in translation elongation. However, the lack of ...Ribosomes translate RNA into proteins. The protein synthesis inhibitor cycloheximide (CHX) is widely used to inhibit eukaryotic ribosomes engaged in translation elongation. However, the lack of structural data for actively translating polyribosomes stalled by CHX leaves unanswered the question of which elongation step is inhibited. We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating ribosomes bound with CHX at 2.7-Å resolution. The ribosome structure from this filamentous fungus contains clearly resolved ribosomal protein eL28, like higher eukaryotes but unlike budding yeast, which lacks eL28. Despite some differences in overall structures, the ribosomes from , yeast, and humans all contain a highly conserved CHX binding site. We also sequenced classic CHX-resistant alleles. These mutations, including one at a residue not previously observed to affect CHX resistance in eukaryotes, were in the large subunit proteins uL15 and eL42 that are part of the CHX-binding pocket. In addition to A-site transfer RNA (tRNA), P-site tRNA, messenger RNA, and CHX that are associated with the translating ribosome, spermidine is present near the CHX binding site close to the E site on the large subunit. The tRNAs in the peptidyl transferase center are in the A/A site and the P/P site. The nascent peptide is attached to the A-site tRNA and not to the P-site tRNA. The structural and functional data obtained show that CHX arrests the ribosome in the classical PRE translocation state and does not interfere with A-site reactivity.
History
DepositionJun 25, 2021-
Header (metadata) releaseDec 1, 2021-
Map releaseDec 1, 2021-
UpdateDec 1, 2021-
Current statusDec 1, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.5
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.5
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7r81
  • Surface level: 0.5
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_24307.png

Downloads & links

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Map

FileDownload / File: emd_24307.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 0.5 / Movie #1: 0.5
Minimum - Maximum-2.0006697 - 4.039758
Average (Standard dev.)0.0019136198 (±0.13177417)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 407.03998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z407.040407.040407.040
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-2.0014.0400.002

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Supplemental data

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Mask #1

Fileemd_24307_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_24307_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_24307_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of the translating Neurospora crassa ribosome arrested ...

EntireName: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
Components
  • Complex: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
    • Complex: 60S ribosomal subunit
      • RNA: 26S rRNA
      • RNA: 5S rRNA5S ribosomal RNA
      • RNA: 5.8S rRNA5.8S ribosomal RNA
      • Protein or peptide: 60S ribosomal protein L2
      • Protein or peptide: 60S ribosomal protein L3
      • Protein or peptide: 60S ribosomal protein L4-ARibosome
      • Protein or peptide: 60S ribosomal protein L5
      • Protein or peptide: 60S ribosomal protein L6
      • Protein or peptide: 60S ribosomal protein L7
      • Protein or peptide: 60S ribosomal protein L8
      • Protein or peptide: 60S ribosomal protein L9
      • Protein or peptide: 60S ribosomal protein L10
      • Protein or peptide: 60S ribosomal protein L11
      • Protein or peptide: 60S ribosomal protein L13
      • Protein or peptide: Ribosomal protein L14
      • Protein or peptide: 60S ribosomal protein L15
      • Protein or peptide: 60S ribosomal protein L16
      • Protein or peptide: 60S ribosomal protein L17
      • Protein or peptide: 60S ribosomal protein L18
      • Protein or peptide: Ribosomal protein L19
      • Protein or peptide: 60S ribosomal protein L20
      • Protein or peptide: Ribosomal protein Srp1
      • Protein or peptide: 60S ribosomal protein L22
      • Protein or peptide: 60S ribosomal protein L23
      • Protein or peptide: 60S ribosomal protein L24
      • Protein or peptide: 60S ribosomal protein L25
      • Protein or peptide: Ribosomal protein L26
      • Protein or peptide: 60S ribosomal protein L27 (eL27)Ribosome
      • Protein or peptide: 60S ribosomal protein L28
      • Protein or peptide: 60S ribosomal protein L29
      • Protein or peptide: 60S ribosomal protein L30
      • Protein or peptide: 60s ribosomal protein
      • Protein or peptide: 60S ribosomal protein L32
      • Protein or peptide: 60S ribosomal protein L33
      • Protein or peptide: 60S ribosomal protein L34
      • Protein or peptide: 60S ribosomal protein L35
      • Protein or peptide: 60S ribosomal protein L36
      • Protein or peptide: Ribosomal protein L37
      • Protein or peptide: 60S ribosomal protein L38
      • Protein or peptide: 60S ribosomal protein L39
      • Protein or peptide: 60S ribosomal protein L40
      • Protein or peptide: hypothetical protein NCU16635 (eL41)Hypothesis
      • Protein or peptide: 60S ribosomal protein L44
      • Protein or peptide: 60S ribosomal protein L43
      • Protein or peptide: Ribosomal_L28e domain-containing protein
    • Complex: 40S ribosomal subunit
      • RNA: 18S rRNA18S ribosomal RNA
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: 40S ribosomal protein S1
      • Protein or peptide: 40S ribosomal protein S2
      • Protein or peptide: Cytoplasmic ribosomal protein 10
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: 40S ribosomal protein S5
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: 40S ribosomal protein S9
      • Protein or peptide: Probable 40s ribosomal protein s10-b
      • Protein or peptide: 40S ribosomal protein S11
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: 40S ribosomal protein S13
      • Protein or peptide: 40S ribosomal protein S14 (uS11)Ribosome
      • Protein or peptide: Ribosomal protein S12
      • Protein or peptide: 40S ribosomal protein S16
      • Protein or peptide: 40S ribosomal protein S17
      • Protein or peptide: 40S ribosomal protein S18
      • Protein or peptide: 40S ribosomal protein S19
      • Protein or peptide: 40S ribosomal protein S20
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: 40S ribosomal protein S22
      • Protein or peptide: 40S ribosomal protein S23
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: 40S ribosomal protein S25
      • Protein or peptide: 40S ribosomal protein S26ERibosome
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S28
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
      • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Complex: tRNATransfer RNA
      • RNA: tRNATransfer RNA
    • Complex: Nascent peptide
      • Protein or peptide: Nascent peptide
    • Complex: mRNAMessenger RNA
      • RNA: mRNAMessenger RNA
  • Ligand: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione
  • Ligand: SPERMIDINE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Structure of the translating Neurospora crassa ribosome arrested ...

SupramoleculeName: Structure of the translating Neurospora crassa ribosome arrested by cycloheximide
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#82

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Supramolecule #2: 60S ribosomal subunit

SupramoleculeName: 60S ribosomal subunit / type: complex / ID: 2 / Parent: 1
Macromolecule list: #1, #3, #5, #7, #9, #11, #13, #15, #17, #19, #21, #23, #25, #27, #29, #31, #33, #35, #37, #39, #41, #43, #45, #47, #49, #51, #53, #55, #57, #59, #61, #63, #65, #67, #69-#79
Source (natural)Organism: Neurospora crassa (fungus)

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Supramolecule #3: 40S ribosomal subunit

SupramoleculeName: 40S ribosomal subunit / type: complex / ID: 3 / Parent: 1
Macromolecule list: #2, #4, #6, #8, #10, #12, #14, #16, #18, #20, #22, #24, #26, #28, #30, #32, #34, #36, #38, #40, #42, #44, #46, #48, #50, #52, #54, #56, #58, #60, #62, #64, #66, #68
Source (natural)Organism: Neurospora crassa (fungus)

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Supramolecule #4: tRNA

SupramoleculeName: tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #80
Source (natural)Organism: Neurospora crassa (fungus)

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Supramolecule #5: Nascent peptide

SupramoleculeName: Nascent peptide / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #81
Source (natural)Organism: Neurospora crassa (fungus)

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Supramolecule #6: mRNA

SupramoleculeName: mRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #82
Source (natural)Organism: Neurospora crassa (fungus)

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Macromolecule #1: 26S rRNA

MacromoleculeName: 26S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 1.07843175 MDa
SequenceString: GGUUGACCUC GGAUCAGGUA GGAAUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACAGGGAUUG CCCUAGUAA CGGCGAGUGA AGCGGCAACA GCUCAAAUUU GAAAUCUGGC UUCGGCCCGA GUUGUAAUUU GUAGAGGAAG C UUUUGGUG ...String:
GGUUGACCUC GGAUCAGGUA GGAAUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACAGGGAUUG CCCUAGUAA CGGCGAGUGA AGCGGCAACA GCUCAAAUUU GAAAUCUGGC UUCGGCCCGA GUUGUAAUUU GUAGAGGAAG C UUUUGGUG AGGCACCUUC UGAGUCCCCU GGAACGGGGC GCCAUAGAGG GUGAGAGCCC CGUAUAGUCG GAUGCCGAUC CA AUGUAAA GCUCCUUCGA CGAGUCGAGU AGUUUGGGAA UGCUGCUCAA AAUGGGAGGU AAAUUUCUUC UAAAGCUAAA UAC CGGCCA GAGACCGAUA GCGCACAAGU AGAGUGAUCG AAAGAUGAAA AGCACUUUGA AAAGAGGGUU AAAUAGCACG UGAA AUUGU UGAAAGGGAA GCGUUUGUGA CCAGACUUGC GCCGUUCCGA UCAUCCGGUG UUCUCACCGG UGCACUCGGG ACGGC UCAG GCCAGCAUCG GUUUUGGCGG GGGGAUAAAG GUCCGGGGAA CGUAGCUCCU CCGGGAGUGU UAUAGCCCCG GGCGUA AUG CCCUCGCCGG GACCGAGGUU CGCGCAUCUG CAAGGAUGCU GGCGUAAUGG UCAUCAACGA CCCGUCUUGA AACACGG AC CAAGGAGUCA AGGUUUUGCG CGAGUGUUUG GGUGUAAAAC CCGCACGCGU AAUGAAAGUG AACGUAGGUG AGAGCUUC G GCGCAUCAUC GACCGAUCCU GAUGUAUUCG GAUGGAUUUG AGUAAGAGCG UUAAGCCUUG GACCCGAAAG AUGGUGAAC UAUGCUUGGA UAGGGUGAAG CCAGAGGAAA CUCUGGUGGA GGCUCGCAGC GGUUCUGACG UGCAAAUCGA UCGUCAAAUC UGAGCAUGG GGGCGAAAGA CUAAUCGAAC CAUCUAGUAG CUGGUUACCG CCGAAGUUUC CCUCAGGAUA GCAGUGUUGU U CUUCAGUU UUAUGAGGUA AAGCGAAUGA UUAGGGACUC GGGGGCGCUU UUUAGCCUUC AUCCAUUCUC AAACUUUAAA UA UGUAAGA AGCCCUUGUU ACUUAAUUGA ACGUGGGCAU UCGAAUGUAC CAACACUAGU GGGCCAUUUU UGGUAAGCAG AAC UGGCGA UGCGGGAUGA ACCGAACGCG GGGUUAAGGU GCCGGAGUGG ACGCUCAUCA GACACCACAA AAGGCGUUAG UACA UCUUG ACAGCAGGAC GGUGGCCAUG GAAGUCGGAA UCCGCUAAGG ACUGUGUAAC AACUCACCUG CCGAAUGUAC UAGCC CUGA AAAUGGAUGG CGCUCAAGCG UCCCACCCAU ACCCCGCCCU CAGGGUAGAA ACGAUGCCCU GAGGAGUAGG CGGCCG UGG AGGUCAGUGA CGAAGCCUAG GGCGUGAGCC CGGGUCGAAC GGCCUCUAGU GCAGAUCUUG GUGGUAGUAG CAAAUAC UU CAAUGAGAAC UUGAAGGACC GAAGUGGGGA AAGGUUCCAU GUGAACAGCG GUUGGACAUG GGUUAGUCGA UCCUAAGC C AUAGGGAAGU UCCGUUUCAA AGGGGCACUU GUGCCCCGUG UGGCGAAAGG GAAGCCGGUU AACAUUCCGG CACCUGGAU GUGGGUUUUG CGCGGCAACG CAACUGAACG CGGAGACGAC GGCGGGGGCC CCGGGCAGAG UUCUCUUUUC UUCUUAACGG UCUAUCACC CUGAAAACAG UUUGUCUGGA GAUAGGGUUU AAUGGCCGGA AGAGCCCGAC ACUUCUGUCG GGUCCGGUGC G CUCUCGAC GUCCCUUGAA AAUCCGCGGG AGGGAAUAAU UCUCACGCCA GGUCGUACUC AUAACCGCAG CAGGUCUCCA AG GUGAACA GCCUCUGGUU GAUAGAACAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGUAACUUCG GGAAAAGGAU UGG CUCUAA GGGUUGGGUA CGUCGGGCUU UGGGCAGACG CCCUGGGAGC AGAUCGCCAC UAGCCGGGCA ACCGGCCGGC GGCU UUCAG CAUCCGGGUG CAGAAGCCCU UAGCAGACUU CGGUCGUCCG GCGUACGUUU AACAACCAAC UUAGAACUGG UACGG ACAG GGGGAAUCUG ACUGUCUAAU UAAAACAUAG CAUUGCGAUG GCCAGAAAGU GGUGUUGACG CAAUGUGAUU UCUGCC CAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA CCAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAG CC AAAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU UAACGAGAUU CCCACUGUCC CUAUCUACUA UCUAGCGA A ACCACAGCCA AGGGAACGGG CUUGGCAGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUU UGACAUUGU GAAAAGACAU AGGAGGUGUA GAAUAGGUGG GAGCUUCGGC GCCGGUGAAA UACCACUACU CCUAUUGUUU UUUUACUUAU UCAAUUAAG CGGGGCUGGA UUUUCGUCCA ACUUCUGGUU UUAAGGUCCU UCGCGGGCCG ACCCGGGUUG AAGACAUUGU C AGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC CAUAACGCAG GUGUCCUAAG GGGGGCUCAU GGAGAACAGA AA UCUCCAG UAGAACAAAA GGGUAAAAGU CCCCUUGAUU UUGAUUUUCA GUGUGAAUAC AAACCAUGAA AGUGUGGCCU AUC GAUCCU UUAGUCCCUC GAAAUUUGAG GCUAGAGGUG CCAGAAAAGU UACCACAGGG AUAACUGGCU UGUGGCGGCC AAGC GUUCA UAGCGACGUC GCUUUUUGAU CCUUCGAUGU CGGCUCUUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAU UGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACCGUCG UGAGACAGGU UAGUUUUACC CUACUGAUGA CCUCAU CGC AAUGGUAAUU GAGCUUAGUA CGAGAGGAAC CGCUCAUUCA GAUAAUUGGU UUUUGCGGUU GUCCGACCGG GCAGUGC CG CGACGCUACC AUCUGCUGGA UAAUGGCUGA ACGCCUCUAA GUCAGAAUCC AUGCCAGAAC GCGAUGAUAC CUCCAGCA C GUUGUAGACG UAUAAGAAUA GGCUCCGGCC UCGUAUCUUA GCAGGCGAUU CCUCCGUUGG CCUUGAAGUG GCCAGCGGU AAUUCGCGUA UUGUAAUUUU AACACGCGCU GGAUCAAAUC CUUUGCAGAC GACUUAGAUG UGCGAAAGGG UCCUGUAAGC AGUAGAGUA GCCUUGUUGU UACGAUCUGC UGAGGGUAAG CCCUCCUUCG CCUAGAUUU

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Macromolecule #2: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 578.821625 KDa
SequenceString: UACCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUUUAAGC AAUUAAACCG CGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUAGUUUAU UUGAUAGUAC CUUACUACAU GGAUAACCGU GGUAAUUCUA G AGCUAAUA ...String:
UACCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUUUAAGC AAUUAAACCG CGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUAGUUUAU UUGAUAGUAC CUUACUACAU GGAUAACCGU GGUAAUUCUA G AGCUAAUA CAUGCUAAAA ACCCCGACUU CGGAAGGGGU GUAUUUAUUA GAUUAAAAAC CAAUGCCCUU CGGGGCUAAC UG GUGAUUC AUAAUAACUU CUCGAAUCGC AUGGCCUUGC GCUGGCGAUG GUUCAUUCAA AUUUCUGCCC UAUCAACUUU CGA CGGCUG GGUCUUGGCC AGCCAUGGUG ACAACGGGUA ACGGAGGGUU AGGGCUCGAC CCCGGAGAAG GAGCCUGAGA AACG GCUAC UACAUCCAAG GAAGGCAGCA GGCGCGCAAA UUACCCAAUC CCGACACGGG GAGGUAGUGA CAAUAAAUAC UGAUA CAGG GCUCUUUUGG GUCUUGUAAU UGGAAUGAGU ACAAUUUAAA UCCCUUAACG AGGAACAAUU GGAGGGCAAG UCUGGU GCC AGCAGCCGCG GUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGUUGAGG UUAAAAAGCU CGUAGUUGAA CCUUGGG CU CGGCCCGUCG GUCCGCCUCA CCGCGUGCAC UGACUGGGUC GGGCCUUUUU UCCUGGAGAA CCGCAUGCCC UUCACUGG G UGUGUCGGGG AACCAGGACU UUUACCGUGA ACAAAUCAGA UCGCUCAAAG AAGGCCUAUG CUCGAAUGUA CUAGCAUGG AAUAAUAGAA UAGGACGUGU GGUUCUAUUU UGUUGGUUUC UAGGACCGCC GUAAUGAUUA AUAGGGACAG UCGGGGGCAU CAGUAUUCA AUUGUCAGAG GUGAAAUUCU UGGAUUUAUU GAAGACUAAC UACUGCGAAA GCAUUUGCCA AGGAUGUUUU C AUUAAUCA GGAACGAAAG UUAGGGGAUC GAAGACGAUC AGAUACCGUC GUAGUCUUAA CCAUAAACUA UGCCGAUUAG GG AUCGGAC GGUGUUAUUU UUUGACCCGU UCGGCACCUU ACGAUAAAUC AAAAUGUUUG GGCUCCUGGG GGAGUAUGGU CGC AAGGCU GAAACUUAAA GAAAUUGACG GAAGGGCACC ACCAGGGGUG GAGCCUGCGG CUUAAUUUGA CUCAACACGG GGAA ACUCA CCAGGUCCAG ACACGAUGAG GAUUGACAGA UUGAGAGCUC UUUCUUGAUU UCGUGGGUGG UGGUGCAUGG CCGUU CUUA GUUGGUGGAG UGAUUUGUCU GCUUAAUUGC GAUAACGAAC GAGACCUUAA CCUGCUAAAU AGCCCGUAUU GCUUUG GCA GUACGCUGGC UUCUUAGAGG GACUAUCGGC UCAAGCCGAU GGAAGUUUGA GGCAAUAACA GGUCUGUGAU GCCCUUA GA UGUUCUGGGC CGCACGCGCG CUACACUGAC ACAGCCAGCG AGUACUCCCU UGGCCGGAAG GUCCGGGUAA UCUUGUUA A ACUGUGUCGU GCUGGGGAUA GAGCAUUGCA AUUAUUGCUC UUCAACGAGG AAUCCCUAGU AAGCGCAAGU CAUCAGCUU GCGUUGAUUA CGUCCCUGCC CUUUGUACAC ACCGCCCGUC GCUACUACCG AUUGAAUGGC UCAGUGAGGC UUCCGGACUG GCCCAGGGA GGUCGGCAAC GACCACCCAG GGCCGGAAAG CUAUCCAAAC UCGGUCAUUU AGAGGAAGUA AAAGUCGUAA C AAGGUCUC CGUUGGUGAA CCAGCGGAGG GAUCAUUA

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Macromolecule #3: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 38.579906 KDa
SequenceString:
ACAUACGACC AUACCCACUG GAAAACUCGG GAUCCCGUCC GCUCUCCCAU AGAUAAGCCA GUGAGGGCCA GACUAGUAGU UGGGUCGGU GACGACCAGC GAAUCCCUGG UGUUGUAUGU U

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Macromolecule #5: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 50.770008 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUG GCAUCGAUGA AGAACGCAGC GAAAUGCGAU AGGUAAUGUG AAUUGCAGAA UUCAGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCUC GCCAGUAUUC UGGCGAGCAU GCCUGUUCGA GCGUCAUUU

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Macromolecule #80: tRNA

MacromoleculeName: tRNA / type: rna / ID: 80
Details: One molecule is found in the A-site, and one molecule is found in the P-site. No specific tRNAs are represented in the peptidyltransferase centers of polysomal ribosomes. Therefore, we ...Details: One molecule is found in the A-site, and one molecule is found in the P-site. No specific tRNAs are represented in the peptidyltransferase centers of polysomal ribosomes. Therefore, we adapted the tRNA model for a eukaryotic ICG-anticodon tRNA that decodes Arg codons (PDB ID: 6T4Q) to model the tRNA densities of both A- and P-site tRNAs.
Number of copies: 2
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 24.468551 KDa
SequenceString:
UUCCUCGUGG CCCAAUGGUC ACGGCGUCUG GCUACGAACC AGAAGAUUCC AGGUUCAAGU CCUGGCGGGG AAGCCA

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Macromolecule #82: mRNA

MacromoleculeName: mRNA / type: rna / ID: 82
Details: No specific mRNA is represented in the polysomal ribosomes. Therefore, we used Uracil to model the mRNA.
Number of copies: 1
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 5.159861 KDa
SequenceString:
UUUUUUUUUU UUUUUUU

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Macromolecule #4: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 31.529408 KDa
SequenceString: MAPANLPSIF NATSTDIEQL LAAQCHIGSK NLGVHMQPYL WKTRADGVNI LNVGKTWEKI VLAARIIAAI DNPADICVIS ARPYGQRAV LKFAAHTGAQ AIAGRFTPGS FTNYITRSFK EPRLIIVTDP RTDAQAIKEA SYVNIPVIAL CDADSPTEYV D VAIPTNNK ...String:
MAPANLPSIF NATSTDIEQL LAAQCHIGSK NLGVHMQPYL WKTRADGVNI LNVGKTWEKI VLAARIIAAI DNPADICVIS ARPYGQRAV LKFAAHTGAQ AIAGRFTPGS FTNYITRSFK EPRLIIVTDP RTDAQAIKEA SYVNIPVIAL CDADSPTEYV D VAIPTNNK GRHAIGCVWW MLAREVLRLR GTIYNRETPW DVMVDLYFYR DPEAEAEEKV EEEKLPGVEE EGVAAIESGF PA TGDWEAA PAGFPATGEW SDAAPGAAAP NWDATAPATT ADWAATEAKE SSW

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Macromolecule #6: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 29.12384 KDa
SequenceString: MAVGKNKRLS KGKKGLKKKT QDPFARKDWY GIKAPAPFNV RDVGKTLVNR TTGLKNANDA LKGRIFEVSL ADLQKDEDHS FRKVKLRVD EIQGKNCLTN FHGLDFTSDK LRSLVRKWQT LIEANITVKT TDDYLLRLFA IAFTKRRPNQ VKKTTYAASS Q IRAIRRKM ...String:
MAVGKNKRLS KGKKGLKKKT QDPFARKDWY GIKAPAPFNV RDVGKTLVNR TTGLKNANDA LKGRIFEVSL ADLQKDEDHS FRKVKLRVD EIQGKNCLTN FHGLDFTSDK LRSLVRKWQT LIEANITVKT TDDYLLRLFA IAFTKRRPNQ VKKTTYAASS Q IRAIRRKM TEIIQREASS CTLTQLTSKL IPEVIGREIE KATQGIYPLQ NVHIRKVKLL KQPKFDLGAL MSLHGESGSD EA GQKVERE FKETVLESV

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Macromolecule #7: 60S ribosomal protein L2

MacromoleculeName: 60S ribosomal protein L2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 27.410562 KDa
SequenceString: MGRVIRNQRK GRGSIFTANT RLNKAPAKFR SLDYAERHGY IRGVVKEIIH DPGRGAPLAR VVFNSPYKFK KQRETFIANE GMYTGQFIY AGKNAALTVG NVLPLGSVPE GTVVSNVEEK VGDRGAIGRT SGNYVTVVGH NPEEGKTRIK LPSGAKKVVS S SARGMIGI ...String:
MGRVIRNQRK GRGSIFTANT RLNKAPAKFR SLDYAERHGY IRGVVKEIIH DPGRGAPLAR VVFNSPYKFK KQRETFIANE GMYTGQFIY AGKNAALTVG NVLPLGSVPE GTVVSNVEEK VGDRGAIGRT SGNYVTVVGH NPEEGKTRIK LPSGAKKVVS S SARGMIGI VAGGGRTDKP LLKASRAKHK FAVKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRY AAQGQKAGLI AA RRTGLLR GTQKTKE

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Macromolecule #8: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 28.80733 KDa
SequenceString: MADRGTSGPR GGGFASRGDR GGDRGRGRGG RGRGRRGGKS EEKEWQPVTK LGRLVKAGKI KSMEEIYLHS LPIKEYQIVD FFLPKLKDE VMKIKPVQKQ TRAGQRTRFK AIVIIGDSEG HVGLGIKTSK EVATAIRAAI IIAKLSVIPV RRGYWGANLG L PHSLPVKE ...String:
MADRGTSGPR GGGFASRGDR GGDRGRGRGG RGRGRRGGKS EEKEWQPVTK LGRLVKAGKI KSMEEIYLHS LPIKEYQIVD FFLPKLKDE VMKIKPVQKQ TRAGQRTRFK AIVIIGDSEG HVGLGIKTSK EVATAIRAAI IIAKLSVIPV RRGYWGANLG L PHSLPVKE SGKCGSVTVR LIPAPRGTSL VASPAVKRLL QLAGIEDAYT SSSGSTKTLE NTLKATFAAV SNTYGFLTPN LW KETKLTR SPLEEFADTL REGKRYAN

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Macromolecule #9: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 44.131371 KDa
SequenceString: MSHRKYEAPR HGSLAYLPRK RAARHRGKVK SFPKDDAKKP VHLTAAMGYK AGMTTIVRDL DRPGAKAHKK EVVEAVTIID TPPMIVVGL VGYIETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYVKKHSD NNGAAITREL ERIKKYCTVV R VLAHTQIR ...String:
MSHRKYEAPR HGSLAYLPRK RAARHRGKVK SFPKDDAKKP VHLTAAMGYK AGMTTIVRDL DRPGAKAHKK EVVEAVTIID TPPMIVVGL VGYIETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYVKKHSD NNGAAITREL ERIKKYCTVV R VLAHTQIR KTPLKQKKAH LMEIQINGGS VADKVEFGHG LFEKPVSIDS IFEKDEVIDV IAVTKGHGFT GVTARWGTKK LP RKTHKGL RKVACIGAWH PSHVQWTVAR AGQAGYHHRT SVNHKIYRIG KGDAEDSAAT EVDVTKKKIT PMGGFVRYGE INN DFVMVK GSVPGVKKRV MTLRKSMFVH TSRKALEKVE LKWIDTSSEF GHGAFQTPAE KKQFQGTLKK DLAASS

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Macromolecule #10: Cytoplasmic ribosomal protein 10

MacromoleculeName: Cytoplasmic ribosomal protein 10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 28.706771 KDa
SequenceString: MAVPGTQISK RRKFVADGVF YAELNEFFQR ELAEEGYSGV EVRVTPTVTD IIIRATHTQE VLGEQGRRIR ELTSLIQKRF KFPENSVSL YAAKVQNRGL SAVAQCESLR YKLLNGLAVR RACYGVLRFI MESGAKGCEV VVSGKLRAAR AKSMKFTDGF M IHSGQPAK ...String:
MAVPGTQISK RRKFVADGVF YAELNEFFQR ELAEEGYSGV EVRVTPTVTD IIIRATHTQE VLGEQGRRIR ELTSLIQKRF KFPENSVSL YAAKVQNRGL SAVAQCESLR YKLLNGLAVR RACYGVLRFI MESGAKGCEV VVSGKLRAAR AKSMKFTDGF M IHSGQPAK DFIDSATRHV LLRQGVLGIK VKIMRGSDPE GKSGPQKSLP DAVTIIEPKE EQPVTQPISQ DYGAKAAQVQ AA AEAARQE EQAGEEEAAA PAAEE

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Macromolecule #11: 60S ribosomal protein L4-A

MacromoleculeName: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 38.882977 KDa
SequenceString: MASRPTVTII GADGKATGTT EVLPKVFGAP IRPDIVKHVH TGMAKNKRQP YSVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQA AFGNMCRSGR MFAPTKIWRK WHVKVNQGQK RFATASALAA SAAAPLLMAR GHQVSTVPEV PLVVDSAAFS G VNKTANAL ...String:
MASRPTVTII GADGKATGTT EVLPKVFGAP IRPDIVKHVH TGMAKNKRQP YSVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQA AFGNMCRSGR MFAPTKIWRK WHVKVNQGQK RFATASALAA SAAAPLLMAR GHQVSTVPEV PLVVDSAAFS G VNKTANAL ALLKAVGAGA DVQKAKASKK LRAGKGKLRD RRHRQRRGPL VVYSAETDGK DLIKGFRNIP GVETSPVDAL NL LQLAPGG HLGRFIVWTS AAIKELDAVY ESKKGFQLPA NVVAQADLTR LINSSEIQSV LRTPKGDAKT KRSNVQKKNP LRN KQVLLR LNPYAATFAK EKLGEIKEEG KPQRVPATFK TLLQE

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Macromolecule #12: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 29.654619 KDa
SequenceString: MGRGPKKHQK RLSAPSHWLL DKLSGVYAPR PSAGPHKLRE CMPLIVFVRN RLKYALNYRE TKAIMMQRLI KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRIQDEEA KYKLGKVKRV QLGKGGIPFL VTHDARTIRY PDPLIKVNDT V KINLETGK ...String:
MGRGPKKHQK RLSAPSHWLL DKLSGVYAPR PSAGPHKLRE CMPLIVFVRN RLKYALNYRE TKAIMMQRLI KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRIQDEEA KYKLGKVKRV QLGKGGIPFL VTHDARTIRY PDPLIKVNDT V KINLETGK IEDFVKFDTG AIAMVTGGRN MGRVGVITHR ERHDGGFNII HVKDALDNTF ATRESNVFVI GSEKPWISLP KG KGVKLSI AEERDRRRAN ALAH

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Macromolecule #13: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 34.47077 KDa
SequenceString: MAFHKLVKNS AYYSRFQTKY KRRREGKTDY YARKRLITQA KNKYNAPKYR LVVRFTNRDI ILQIVSSEIT GDKVFASAYS HELKAYGIE HGLTNWAAAY ATGLLLARRV LKKLGLDETF KGVEEADGEY KLTEAAETDD GERRPFKAFL DVGLARTSTG A RVFGAMKG ...String:
MAFHKLVKNS AYYSRFQTKY KRRREGKTDY YARKRLITQA KNKYNAPKYR LVVRFTNRDI ILQIVSSEIT GDKVFASAYS HELKAYGIE HGLTNWAAAY ATGLLLARRV LKKLGLDETF KGVEEADGEY KLTEAAETDD GERRPFKAFL DVGLARTSTG A RVFGAMKG ASDGGIFIPH SENRFPGYDM ESEELDAETL KKYIFGGHVA EYMETLADDD EERYKSQFNR YIEDDLEADG LE DLYAEAH AAIREDPFKK AESEAPKKTK EEWKAESLKY KKSKLTREQR AAGVQERIAA LRSE

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Macromolecule #14: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 23.715213 KDa
SequenceString: MSEGEVDVAA VSQYEVLPKE VLAEVGSVKL FNRWSYEDVE IRDISLTDYI QIRSPVYLPH SAGRYAAKRF RKANCPIIER LTNSLMMHG RNNGKKLMAV RIVAHAFEII HLMTDQNPIQ IAVDAIVNCG PREDSTRIGS AGTVRRQAVD VSPLRRVNQA I ALLTTGAR ...String:
MSEGEVDVAA VSQYEVLPKE VLAEVGSVKL FNRWSYEDVE IRDISLTDYI QIRSPVYLPH SAGRYAAKRF RKANCPIIER LTNSLMMHG RNNGKKLMAV RIVAHAFEII HLMTDQNPIQ IAVDAIVNCG PREDSTRIGS AGTVRRQAVD VSPLRRVNQA I ALLTTGAR EASFRNVKSI AECLAEELIN AAKGSSNSYA IKKKDELERV AKSNR

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Macromolecule #15: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 22.516178 KDa
SequenceString: MSSAAAPQTK TFGKATRTVP HSSEKAQKWY PAEDEAQPRK VRKTIRPWSP RSTLVPGTVL ILLAGRFRGK RVVLLKALDQ GVLLVTGPF KINGVPLRRV NARYVIATSQ KVDLAGIDEA KINEIAQPKY FTAEKSKEKA GEEAFFKQGE KPQKKEVSSS R VADQKAID ...String:
MSSAAAPQTK TFGKATRTVP HSSEKAQKWY PAEDEAQPRK VRKTIRPWSP RSTLVPGTVL ILLAGRFRGK RVVLLKALDQ GVLLVTGPF KINGVPLRRV NARYVIATSQ KVDLAGIDEA KINEIAQPKY FTAEKSKEKA GEEAFFKQGE KPQKKEVSSS R VADQKAID KALIANIKKV EFLASYLASS FTLRKGDKPH EMKW

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Macromolecule #16: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 27.395119 KDa
SequenceString: MKLNISYPAN GSQKLIEVED ERKLRHFIEK RMGAEVPADP LGDEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSDG HSCYRARRT GERKRKSVRG CIVGSDLSVL ALAIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFGLSK DDDVRKYVIR R EVQPKGEG ...String:
MKLNISYPAN GSQKLIEVED ERKLRHFIEK RMGAEVPADP LGDEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSDG HSCYRARRT GERKRKSVRG CIVGSDLSVL ALAIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFGLSK DDDVRKYVIR R EVQPKGEG KKPYTKAPKI QRLVTPQRLQ HKRHRLALKR RQAEKVKEEA SEYAQILAKR IAEAKVQKAD ARKRRASSMR K

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Macromolecule #17: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 28.741695 KDa
SequenceString: MSTSVPTKND VLVPETLLKK RKSQEKARAE RQAEIEKKKA ANKEKRAVIF KRAETYVKEY RDVEREKIRL QRAAKQDGSF HIPAEAKLI FLIRIKGINK IPPKPRKILQ LLRLLQINNG VFVRVTKATA EMIKIVEPWV AYGYPNLKSV KELIYKRGYG K VNKQRVAL ...String:
MSTSVPTKND VLVPETLLKK RKSQEKARAE RQAEIEKKKA ANKEKRAVIF KRAETYVKEY RDVEREKIRL QRAAKQDGSF HIPAEAKLI FLIRIKGINK IPPKPRKILQ LLRLLQINNG VFVRVTKATA EMIKIVEPWV AYGYPNLKSV KELIYKRGYG K VNKQRVAL TDNSIIEENL GKYGIICMED LIHEIYTVGP NFKQASNFLW PFKLSNPTGG FRTRKFKHFI EGGDLGNREE HI NALIRQM N

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Macromolecule #18: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 22.913477 KDa
SequenceString: MSAPQLNKIA ANSPSRQNPS ELETAVAGAL YDLESNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PSLQGFHRVQ QRLTRELEK KFSDRHVLIV ASRRILPRPK RSSRSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRVKEDGSKT L KVILDEKE ...String:
MSAPQLNKIA ANSPSRQNPS ELETAVAGAL YDLESNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PSLQGFHRVQ QRLTRELEK KFSDRHVLIV ASRRILPRPK RSSRSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRVKEDGSKT L KVILDEKE RGSVDYRLDT YSAVYRRLTG KNVLFEFPLV SAEY

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Macromolecule #19: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 29.427127 KDa
SequenceString: MPQKSGKKAA PAPFPQGKAG SKKAPKNPLI EKRPRNFGIG QAIQPKRNLS RMVKWPEYVR LQRQKKILNM RLKVPPALAQ FQQVLDKNT AAQAFKLLNK YRPETKTEKK ERLLQEATAI KEGKKKEDVS KKPYVVKYGL NHVVGLIENK KASLVLIPND V DPIELVVF ...String:
MPQKSGKKAA PAPFPQGKAG SKKAPKNPLI EKRPRNFGIG QAIQPKRNLS RMVKWPEYVR LQRQKKILNM RLKVPPALAQ FQQVLDKNT AAQAFKLLNK YRPETKTEKK ERLLQEATAI KEGKKKEDVS KKPYVVKYGL NHVVGLIENK KASLVLIPND V DPIELVVF LPALCRKMGI PYAIIKGKAR LGTVVHKKTA AVLALTEVRA EDKNELAKLV SAIKEGYLEK NEQARRQWGG GI MGVKSQM KIAKRKKALE NAIKV

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Macromolecule #20: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 23.061273 KDa
SequenceString: MGISRDSRHK RSHTGAKRAY YRKKRAFEAG RQPANTRIGA KRIHTVRTRG GNHKYRALRL DSGNFAWASE GCTRKTRVIV VAYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAER FAAAGKVDPA L EKQFEAGR ...String:
MGISRDSRHK RSHTGAKRAY YRKKRAFEAG RQPANTRIGA KRIHTVRTRG GNHKYRALRL DSGNFAWASE GCTRKTRVIV VAYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAER FAAAGKVDPA L EKQFEAGR LYAVISSRPG QSGRCDGYIL EGEELAFYQR KLHK

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Macromolecule #21: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 21.787223 KDa
SequenceString: MRYIHSEETL EVPENVKVSI KSRIVTVEGP RGKLVKDLSH IAVNFSVIKK GVIGLEIHHG NRKNVAALRT VRTIINNLII GVTKGFKYK MRYVYAHFPI NVNVEKNAET GNFEVEIRNF IGEKIVRRVV MQPGVDVEIS KAQKDELVLS GNSLEGVSQS A ADIQQICR ...String:
MRYIHSEETL EVPENVKVSI KSRIVTVEGP RGKLVKDLSH IAVNFSVIKK GVIGLEIHHG NRKNVAALRT VRTIINNLII GVTKGFKYK MRYVYAHFPI NVNVEKNAET GNFEVEIRNF IGEKIVRRVV MQPGVDVEIS KAQKDELVLS GNSLEGVSQS A ADIQQICR VRNKDIRKFL DGIYVSEKGN IVEDQ

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Macromolecule #22: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 21.852533 KDa
SequenceString:
MAPRSYSKTA KVPRRPFEAA RLDSELKLVG EYGLRNKREV WRVLLTLSKI RRAARQLLTL DEKDPKRLFE GNALIRRLVR VGVLDESRM KLDYVLALKA EDFLERRLQT CVYKLGLAKS IHHARVLIRQ RHIRVGKQIV NVPSFVVRLD SQKHIDFALT S PFGGGRPG RVRRKKAKAA EGGEAAEEED EE

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Macromolecule #23: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 25.373594 KDa
SequenceString: MARRPARCYR YCKNKPYPKS RFNRGVPDPK IRIFDLGRKR ATVDDFPLCI HMVSNEYEQL SSEALEAARI CANKYLVKTA GKEGFHLRV RVHPYHVVRI NKMLSCAGAD RLQTGMRGAW GKPNGTVARV NIGQIIMSVR TRDSNKAFAL EALRRSQYKF P GRQKIIIS ...String:
MARRPARCYR YCKNKPYPKS RFNRGVPDPK IRIFDLGRKR ATVDDFPLCI HMVSNEYEQL SSEALEAARI CANKYLVKTA GKEGFHLRV RVHPYHVVRI NKMLSCAGAD RLQTGMRGAW GKPNGTVARV NIGQIIMSVR TRDSNKAFAL EALRRSQYKF P GRQKIIIS KNWGFTPLRR EEYLEKKAAG KLLIDGAYVQ FLGNRGSLER NMKRFPDAFA TEA

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Macromolecule #24: Probable 40s ribosomal protein s10-b

MacromoleculeName: Probable 40s ribosomal protein s10-b / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 18.463754 KDa
SequenceString:
MLIPKADRKA IHEYLFREGV MVAEKNYEST HETGIRNLYV IKACQSLTSR GYVKTQFSWQ YYYYTLTPEG LDYLREWLHL PAEIVPATH IKSQRSHAPP RGMLGGEERR ERPFGGRGRG DREGGYRRRE AGEGKEGGAP GEFAPQFRGG FGRGRAGRGE A PPS

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Macromolecule #25: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 20.123266 KDa
SequenceString:
MSTEQKAQNP MRELRIQKLV LNICVGESGD RLTRAAKVLE QLSGQTPVYS KARYTVRTFG IRRNEKISVH VTVRGPKAEE ILERGLKVK EYELRKRNFS ETGNFGFGIS EHIDLGIKYD PGIGIYGMDF YCCMTRPGER VSRRRRAKSR VGATHRITRD D TVKWFKSR FDAIVR

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Macromolecule #26: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 18.468691 KDa
SequenceString:
MATELTVQSE RAFQKQPHIF LNSKTKVKSA RPGKGGRRWY KDVGLGFRTP AAAIEGQYID KKCPFTGQVS IRGRILSGTV VSTKMHRTI IIRREYLHFI PKYNRYEKRH KNLAAHVSPA FRVEEGDKVT VGQCRPLSKT VRFNVLRVLP RTGKSVKKFA K F

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Macromolecule #27: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 23.929869 KDa
SequenceString: MAIKHNQQIP NNHFRKDWQR RVRCHFDQAG KKASRRVARQ AKAAALAPRP VDKLRPIVRC PTIKYNRRTR LGRGFSLAEL KAAGIPKLV APTIGISVDP RRANLSEESL AANVERLKAY QARLVVFPRK SNKPKKADTP KDQQTGEFVK SVEAVFGVER P IASGFKEI ...String:
MAIKHNQQIP NNHFRKDWQR RVRCHFDQAG KKASRRVARQ AKAAALAPRP VDKLRPIVRC PTIKYNRRTR LGRGFSLAEL KAAGIPKLV APTIGISVDP RRANLSEESL AANVERLKAY QARLVVFPRK SNKPKKADTP KDQQTGEFVK SVEAVFGVER P IASGFKEI PKSELPSNIE GGAFRALRKA RSDAKLIGVR EKRAKDKAAA EAEKSK

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Macromolecule #28: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.250645 KDa
SequenceString:
MSDVEENTVP ASEEVEVSAD AAPKGQMSVL DALKGVLKIS LMHDGLARGL REASKALDRR EAHMCVLNES CEEEAYKKLV IALCSEHKI PLIKVPDGKQ LGEWAGLCVL DREGNARKVV NCSCVVVKNW GEMSSEREIL LNYFQTEQ

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Macromolecule #29: Ribosomal protein L14

MacromoleculeName: Ribosomal protein L14 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.885671 KDa
SequenceString:
MADIQIGSSA WRLVEVGRVL KLEGGSLATI VEIVDHKRVL VDGPSSDPKL AAPRGVVSLS RTLLTPLVVE KLPRGARTGA VKKAWEAAG IDAKWKESNW AKKQLQQERR KALTDFDRFK VMRLKKQRRF EERKALAKIK ASA

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Macromolecule #30: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.90491 KDa
SequenceString:
MGRMHSKGKG ISASAIPYSR NPPSWLKTTP EQVVDQICKL AKKGATPSQI GVILRDSHGI AQVKVVTGNK ILRILKSNGL APDLPEDLY MLIKKAVAVR KHLERNRKDK DSKFRLILIE SRIHRLARYY KTVGVLPPTW KYESSTASTI VS

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Macromolecule #31: 60S ribosomal protein L15

MacromoleculeName: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 24.250232 KDa
SequenceString: MGALKYLEEL QKKKQSDVVR FLLRVRCWEL RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRARVRRGG RKKPVPKGAT YGKPTNQGV NQLKYQRSLK STAEERVGRR CANLRVLNSY WINQDSTYKY FEVILVDPQH KAIRRDPRIN WIVNPVHKHR E SRGLTSTG ...String:
MGALKYLEEL QKKKQSDVVR FLLRVRCWEL RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRARVRRGG RKKPVPKGAT YGKPTNQGV NQLKYQRSLK STAEERVGRR CANLRVLNSY WINQDSTYKY FEVILVDPQH KAIRRDPRIN WIVNPVHKHR E SRGLTSTG KRSRGLNKGH RYNKTRAGRR KTWKRHNTLS LWRYR

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Macromolecule #32: 40S ribosomal protein S14 (uS11)

MacromoleculeName: 40S ribosomal protein S14 (uS11) / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.025438 KDa
SequenceString:
MPPKKAARPA QENISLGPQI REGELVFGVA RIFASFNDTF VHVTDLSGRE TICRVTGGMK VKADRDESSP YAAMLAAQDV AARCKELGI TALHIKIRAT GGNGTKTPGP GAQSALRALA RSGMKIGRIE DVTPTPSDST RRKGGRRGRR L

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Macromolecule #33: 60S ribosomal protein L16

MacromoleculeName: 60S ribosomal protein L16 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 26.184609 KDa
SequenceString: MSQHQAHGQF SAHTKKSQTV NHRESSPAKM SSFESVVVID GKGHLLGRLA SIVAKQLLNG QKIVVVRCEA LNISGEFFRA KLKYHSYLR KMTRYNPTRG GPFHFRAPSR IFYKAVRGMI PHKTARGAAA LERLKVFEGV PPPYDKKKKM VVPQALRVLR L QPGRKFCT ...String:
MSQHQAHGQF SAHTKKSQTV NHRESSPAKM SSFESVVVID GKGHLLGRLA SIVAKQLLNG QKIVVVRCEA LNISGEFFRA KLKYHSYLR KMTRYNPTRG GPFHFRAPSR IFYKAVRGMI PHKTARGAAA LERLKVFEGV PPPYDKKKKM VVPQALRVLR L QPGRKFCT VGRLSSEVGW KYEDVVSRLE ERRKAKGAAY YERKKVAARQ LSEAKKSAKV NDKTAEALKE FGY

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Macromolecule #34: Ribosomal protein S12

MacromoleculeName: Ribosomal protein S12 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 17.419408 KDa
SequenceString:
MADEYNAEEA AELKKKRTFR KFSYRGIDLD ALLDLGSDEL RDVVHARARR RINRGLKRKP MGLIKKLRKA KQEAKPNEKP DLVKTHLRD MIVVPEMIGS VIGIYSGKEF NQVEIKPEMV GHYLAEFSIS YKPVKHGRPG IGATHSSRFI PLK

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Macromolecule #35: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 20.802846 KDa
SequenceString:
MVRYAATEIA PAKSARSRGS YLRVSFKNTR ETAQAINGWK LQRAQKFLEN VLEKKEAVPM RRYAGGTGRA AQGKQFGVSR ARWPAKSAE FLLGLLKNAE ANADAKGLDT GNLVVKHIQV NQAPKQRRRT YRAHGRINPY MSNPCHIELI LTEADEVVQK S EAVVREEA HLSSRQRGAR VRRAITAA

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Macromolecule #36: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.752556 KDa
SequenceString:
MATQAVQVFG KKKNATAVAR CVQGKGLIKV NGVPLKLYAP EILRAKLYEP ILLLGTDKFA EVDIRLKVSG GGHVSQVYAV RQAIAKAIV AYYAKYVDEH SKNTLKTALI QFDRTLLVAD PRRCEPKKFG GKGARSRFQK SYR

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Macromolecule #37: 60S ribosomal protein L18

MacromoleculeName: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 20.6514 KDa
SequenceString:
MGIDLRKHHE RSTHRKAPKS DNVYLKLLVK LYRFLARRTD SAFNKVVLRR LFMSRINRPP MSISRIVANL KGNEKKTVVV VGTVTDDNR LLTVPKVSVA ALRFTATARA RIEAAGGQAI TLDQLALEKP TGANTLLLRG PKNAREAVKH FGFGPHKHKK P YVASKGRK FEKARGRRRS KGFKV

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Macromolecule #38: 40S ribosomal protein S17

MacromoleculeName: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.974506 KDa
SequenceString:
MGRVRTKTVK KSAKVIIERY YPKLTLDFET NKRICDEIAI IASKRLRNKI AGYTTHLMKR IQRGPVRGIS FKLQEEERER KDQYVPEVS ALDFTQNSES GQLDVDTETK DLLKHLGFDS IPVNVIPVTQ AQPVERGRRF GDRPRRD

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Macromolecule #39: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 22.407271 KDa
SequenceString: MVNLRTQKRL ASSVLGVGQR KIWLDPNEVS EISNANSRQT IRKLVADGLI IKKPVTMHSR SRARELNLAR RIGRHRGFGK RKGTADARM PEQVLWMRRQ RVLRRLLVKY RASGKIDKHL YHELYHLAKG NTFKHKRALV EHIHRAKAEK AREKAIKEEM D AKRARTKA ...String:
MVNLRTQKRL ASSVLGVGQR KIWLDPNEVS EISNANSRQT IRKLVADGLI IKKPVTMHSR SRARELNLAR RIGRHRGFGK RKGTADARM PEQVLWMRRQ RVLRRLLVKY RASGKIDKHL YHELYHLAKG NTFKHKRALV EHIHRAKAEK AREKAIKEEM D AKRARTKA ARERKLERQA AKRNALAGEE EESK

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Macromolecule #40: 40S ribosomal protein S18

MacromoleculeName: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 17.806611 KDa
SequenceString:
MSLVSGEKSN FNHILRLLNT NVDGKQKVVY SLTKIKGVGR RYSNLVCKKA DVDLSKRAGE LTSEELERIV TIMQNPTAYK IPAWFLNRQ RDIVDGKDSQ ILANGVDSKL RDDLERLKKI RAHRGLRHYW GLRVRGQHTK TTGRRGRTVG VSKKKGN

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Macromolecule #41: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 20.385027 KDa
SequenceString:
MGRLQEYQVI GRHLPTESNP TPALYRMRIF APNAVVAKSR FWYFLRGLRK VKKATGEIVT CNVITEKHPL KVKNFGVWIR YDSRSGTHN MYKEYRELSR TAAVEALYSD MAARHRARFR SIHILKVVEL EKTEDIKRPY LKQLVTKKLS FPLPHRVPKI S NKKIFSAK RPATFA

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Macromolecule #42: 40S ribosomal protein S19

MacromoleculeName: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.700889 KDa
SequenceString:
MPGGVTVRDV EPHKFVNAYA AFLKRQGKLP VPGWVDTVKT GPAKEMPPQD IDWFYVRAAS VARHVYLRKT VGVGRLRRVH GTAKNRGSR PSHHVEASGS VDRKVLQALE KIGVLEHDEE KGGRRITQQG QRDLDRIAQT VIEADEEDDE

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Macromolecule #43: Ribosomal protein Srp1

MacromoleculeName: Ribosomal protein Srp1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 18.253395 KDa
SequenceString:
MGHAAGLRAG TRYAFSRNFR EKGMIRLSTY LKQYKVGDIV DIKVNGAVQK GMAHKVYHGK TGVIYNVTKS AVGIIIYKKV KHRYIEKRI NVRIEHIQPS RSRDDFLRRV KQNAELKKQA KAEGKPVQLK RQALLPREAR TISVVDNKPE TVAPVAYETT I

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Macromolecule #44: 40S ribosomal protein S20

MacromoleculeName: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 13.250597 KDa
SequenceString:
MSSYNKDQKD FGEAPKVHRV RITLTSRKVQ ALEKVCSELV ERAKTKDLRV KGPVRLPTKI LKITTRKSPC GEGSKTWDLF EMRIHKRLI DLTAPTEIVK QIIVNIEAGV EVEVTIAA

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Macromolecule #45: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 14.234482 KDa
SequenceString:
MAPITKKSAK GKGPKVTKKF VINASQPASD KIFDVSAFEK FLTEKIKVEG RVGNLGDAIK ISQVGDGKIE IVAHNELSGR YLKYLTKKF LKKMQLRDWL RVVSTSRGVY ELKFFNVVND EAEEDEE

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Macromolecule #46: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 9.674954 KDa
SequenceString:
MENDRGEIVD LYVPRKCSAT GRIIKSKDHG SCQITIAKVD ENGRAIQGEN IIYALSGFVR AMGESDDSLN RLAQRDGLLK AVWNPQR

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Macromolecule #47: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 14.722408 KDa
SequenceString:
MAKQSRGAPG GKLKMTLGLP VGAIMNCADN SGARNLYIIS VKGIGARLNR LPAGGVGDMV MATVKKGKPE LRKKVHPAVI VRQSKPWKR FDGVFLYFED NAGVIVNPKG EMKGSAITGP VGKEAAELWP RIASNSGVVM

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Macromolecule #48: 40S ribosomal protein S22

MacromoleculeName: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 14.846377 KDa
SequenceString:
MVRTSVLHDA LNSINNAEKA GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EEVDDHRSGK IVVQLNGRMN KCGVISPRYN VRLAELEKW VVKLLPARQF GYVILTTSAG IMDHEEARRK HVSGKIIGFF Y

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Macromolecule #49: 60S ribosomal protein L24

MacromoleculeName: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 17.656621 KDa
SequenceString:
MRTYDDTFSG QRIYPGKGKL YVRGDSKIFR FQNGKSESLF LQRKNPRRIA WTVLYRRQHK KGISEEVAKK RSRRTVKAQR AIVGASLEV IKERRSMRPE ARSAARLAAI KESKAKKQET QAAKKAEKAK NAANPKARVT SKQGAKGAPV KVAAKSR

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Macromolecule #50: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.944671 KDa
SequenceString:
MSGGKPRGLN AARKLRNNRR EQRWSDLHYK KRALGTAFKS SPFGGSSHAK GIVLEKVGVE AKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VGLLALWKEK KEKPRS

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Macromolecule #51: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 17.15816 KDa
SequenceString:
MAPKDTKKGG ASKAGKGAQA KKAAQAALKG VHSHKKTKVR YSTTFHRPKT LQLSRAPKYP RKSIPHEPRL DEHKVIVHPL NTEGALKKI EEQNTLVFIV DVKANKAQIK QALKKLYDID TVKINTLIRP DGSKKAFARL TADVDALDIA ATKLGLV

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Macromolecule #52: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.5983 KDa
SequenceString:
MADSDSPVTL RTRKFIRNPL LGRKQMVVDI LHPNRPNISK DELREKLATL YKATKDQVNV FGLRTQFGGG KTTGFALVYD SPEAMKKFE PHYRLVRVGL ASKIEKASRQ QRKQRKNRQK TLRGTAKVKG AKPKKEK

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Macromolecule #53: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.366948 KDa
SequenceString:
MVKVNNSVSS SRRKARKAHF SAPSSVRRNI MSAPLSKELR EKYNVRSIPI RKDDEVTIVR GSNKDKEGKV TSVYRLKYVI HVERVTRDK ATGASVPVGI HPSNVVITKL KLDKDREAIL ERIKRGRELA KPTKVSA

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Macromolecule #54: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 10.822743 KDa
SequenceString:
MAPAASGAKK QKKKWSKGKV KDKAQHAVIL DKSTSDKLYK DVQSYRLVTV ATLVDRLKIN GSLARRCLKD LEEKGQIKQV VGHSKMKIY TRAIGADE

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Macromolecule #55: 60S ribosomal protein L27 (eL27)

MacromoleculeName: 60S ribosomal protein L27 (eL27) / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.75651 KDa
SequenceString:
MKFLKTSRVC LVTRGRYAGK KVVIIQPVDN GSKTHPYGHA IVAGIERYPS KITRRMSKTR QEKRSKVKPF IKVINYNHLM PTRYTLELE GLKGSVSAET FKEVSQREDA KKTVKKVLEE RYTSGKNRWF FTPLRF

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Macromolecule #56: 40S ribosomal protein S26E

MacromoleculeName: 40S ribosomal protein S26E / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 13.580064 KDa
SequenceString:
MVKKRKNNGR NKKGRGHVKP IRCSNCSRCT PKDKAIKRFT IRNMVESAAI RDISDASVFA EYTVPKMYLK LQYCVSCAIH GKIVRVRSR EGRRNRAPPP RVRYNKDGKK VTPTQGAKTA

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Macromolecule #57: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.636375 KDa
SequenceString:
MPTRFSKTRK HRGHVSAGKG RVGKHRKHPG GRGMAGGQHH HRTNLDKYHP GYFGKVGMRH FHLLRNHQWA PILNIEKLWT LVPAEAREK YVSGAATETA PVIDLLSHGY AKLLGKGRLP QVPIVVRARY VSAEAERKIK EAGGVIELVA

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Macromolecule #58: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 8.910573 KDa
SequenceString:
MVLAVDLLNP SPASEARKHK LKTLVPAPRS FFMDVKCPGC FTITTVFSHA QTVVICQGCT TVLCQPTGGK ARLTEGCSFR RK

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Macromolecule #59: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 7.488608 KDa
SequenceString:
MAKSKNSSQH NQSRKAHRNG IKKPKTSRYP SLKGTDPKFR RNHRHALHGT AKALKEFKEG KRETA

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Macromolecule #60: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 7.735974 KDa
SequenceString:
MDSSKAPVKL VKVTRVLGRT GSRGGVTQVR VEFMDDQTRS IIRNVKGPVR EDDILVLLES EREARRLR

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Macromolecule #61: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 11.720708 KDa
SequenceString:
MAPKKSKSDA QSIGAKLALV IKSGKVVLGY RSTLKALRSG KAKLILISAN TPPLRKSELE YYSMMSKTAV HHYTGTNIEL GTACGKLFR CSTMAILDAG DSDILADQQQ

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Macromolecule #62: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 6.515507 KDa
SequenceString:
MSHESVWNSR PRTYGKGSRS CRVCTHSAGL IRKYGLNICR QCFREKANDI GFTKHR

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Macromolecule #63: 60s ribosomal protein

MacromoleculeName: 60s ribosomal protein / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 14.097444 KDa
SequenceString:
MSSKKPTGKT QRSAIADVVA REYTIHMHKR MHGVTFKKRA PRAIKEIKEF AYKAMGTTDV RIDPQLNKKV WEQGVKGVAY RLRVRISRR RNDEEGAKEK LYSYVQAVNV KNPKGLHTVV VEE

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Macromolecule #64: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 6.912104 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEPQEKPKTP KGRAHKREIY TRRFVNVVTG PGGKRKMNAN PSA

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Macromolecule #65: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 15.001829 KDa
SequenceString:
MVAAKKHVPI VKKRSKGFMR HQSDRFMRVD SAWRKPKGID NRVRRRFRGT LAMPSIGYGS NKKTRHMMPS GHKAFLVSNV KDVELLLMH NKTYAAEIAH NVSSRKRIEI IARAKQLSVK VTNAKAKVTT EV

+
Macromolecule #66: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 17.775771 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKQKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIKHKRKKTK LAVLKYYKVD SDGKIERLRR ECPNETCGAG VFMAAMQDRQ YCGRCHLTYV FEKSS

+
Macromolecule #67: 60S ribosomal protein L33

MacromoleculeName: 60S ribosomal protein L33 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 12.203169 KDa
SequenceString:
MPTEAGHRLY VKGRHLSYQR GRHTTHPKTS LIKIEGVDDT AAANFYLGKR VAYVYRGQKE VRGTKIRVIW GKVTRPHGNS GVVRAKFAA PLPSRSFGAS VRIMLYPSSI

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Macromolecule #68: Guanine nucleotide-binding protein subunit beta-like protein

MacromoleculeName: Guanine nucleotide-binding protein subunit beta-like protein
type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 35.173578 KDa
SequenceString: MAEQLILKGT LEGHNGWVTS LATSLENPNM LLSGSRDKSL IIWNLTRDET SYGYPKRRLH GHSHIVSDCV ISSDGAYALS ASWDKTLRL WELSTGTTTR RFVGHTNDVL SVSFSADNRQ IVSGSRDRTI KLWNTLGDCK FTITEKGHTE WVSCVRFSPN P QNPVIVSS ...String:
MAEQLILKGT LEGHNGWVTS LATSLENPNM LLSGSRDKSL IIWNLTRDET SYGYPKRRLH GHSHIVSDCV ISSDGAYALS ASWDKTLRL WELSTGTTTR RFVGHTNDVL SVSFSADNRQ IVSGSRDRTI KLWNTLGDCK FTITEKGHTE WVSCVRFSPN P QNPVIVSS GWDKLVKVWE LSSCKLQTDH IGHTGYINAV TISPDGSLCA SGGKDGTTML WDLNESKHLY SLNANDEIHA LV FSPNRYW LCAATSSSII IFDLEKKSKV DELKPEFQNI GKKSREPECV SLAWSADGQT LFAGYTDNII RAWGVMSRA

+
Macromolecule #69: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 13.225604 KDa
SequenceString:
MASSRVTYRR RNPYNTTSNK TRIVKTPGGQ LRVLHIKKRG TAPKCGDCGI KLPGVPALRP REYAQLSKPK KTVQRAYGGS RCGNCVRDR VVRAFLIEEQ KIVKKVLKEQ SQAEKSKK

+
Macromolecule #70: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 14.445221 KDa
SequenceString:
MSSGKVKAGA LWSKNKDELT KQLGELKTEL GQLRIQKIVS SGTKLNKIHD LRKSIARVLT IINAKQRAQL RLFYKNKKYL PLDLRAKQT RAIRRRLSKE DASRVLEKTK KRTTHFPLRK FAVKAA

+
Macromolecule #71: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 11.587708 KDa
SequenceString:
MAAESKTGLA VGLKKGHKTT ARVSKPRVSR TKGHLSKRTA FVRSIVQEVA GLAPYERRVI ELLRNSKDKR ARKLAKKRLG TFGRAKAKV EDLQRVIAEA RRAGH

+
Macromolecule #72: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 10.261002 KDa
SequenceString:
MTKGTSSFGK RHTKTHGLCR RCGRRSLHNQ KKVCASCGYP AAKTRKYNWS EKAKRRKTTG TGRLRYLSTV SRKFKNGFQT GVPKGSKGP VAA

+
Macromolecule #73: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 9.169931 KDa
SequenceString:
MPQEIGDIKK FIEICRRKDA SAARIKKNKA TQQIKFKVRC QKYLYTLVLK DSDKAEKLKA SLPPSLTIAD VAKRNKKQTA

+
Macromolecule #74: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 6.283508 KDa
SequenceString:
MPSHKTFRVK QKLAKAQKQN RPIPQWIRLR TGNTIRYNAK RRHWRKTRLG L

+
Macromolecule #75: 60S ribosomal protein L40

MacromoleculeName: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 6.092504 KDa
SequenceString:
MIEPSLKALA SKFNCDKMIC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

+
Macromolecule #76: hypothetical protein NCU16635 (eL41)

MacromoleculeName: hypothetical protein NCU16635 (eL41) / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 3.384335 KDa
SequenceString:
MRAKWRKKRV RRLKRKRRKM RARSK

+
Macromolecule #77: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 11.945273 KDa
SequenceString:
MVNVPKTRKT YCAGRSCGKH TLHKVTQYKA GKASAFAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKVVL RLECSVCKQK KQLPLKRCK HFELGGDKKT KGAALVF

+
Macromolecule #78: 60S ribosomal protein L43

MacromoleculeName: 60S ribosomal protein L43 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 10.154967 KDa
SequenceString:
MSKRTKKVGI SGKYGTRYGA SLRKLVKKQE VSQHARYTCT FCGKNTVRRS SVGIWSCKAC KKTLAGGAYT VSTPAAAAMR STLRRLREI TEV

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Macromolecule #79: Ribosomal_L28e domain-containing protein

MacromoleculeName: Ribosomal_L28e domain-containing protein / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 16.011481 KDa
SequenceString:
MSLSNVSADL IWEITRSHNS FLVKRKSGGG VQFSRDPLNL TNKHSRKYAG FVNDKAVGVV PGEKGGVVVT TKKAGSINKP AARYTVAFG ANKTARKTYK AIARQVAGYR SDLLKAAVAR ASAIRRSQRA VKPEPAQKLR GNAAKKAAAS A

+
Macromolecule #81: Nascent peptide

MacromoleculeName: Nascent peptide / type: protein_or_peptide / ID: 81
Details: No specific nascent peptide is represented in the polysomal ribosomes. Therefore, we used UNK (unknown residue) to represent each residue of the nascent peptide.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa (fungus)
Molecular weightTheoretical: 358.434 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #83: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethy...

MacromoleculeName: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione
type: ligand / ID: 83 / Number of copies: 1 / Formula: 3HE
Molecular weightTheoretical: 281.347 Da
Chemical component information

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione / Cycloheximide

+
Macromolecule #84: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 84 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

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Macromolecule #85: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 85 / Number of copies: 260 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 32.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4u3u


Details: 4U3U and 6T4Q
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 196154
FSC plot (resolution estimation)

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