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基本情報
登録情報 | データベース: EMDB / ID: EMD-23017 | |||||||||
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タイトル | GluK2/K5 apo | |||||||||
![]() | GluK2/K5 apo | |||||||||
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機能・相同性 | ![]() regulation of synaptic vesicle fusion to presynaptic active zone membrane / protein retention in ER lumen / chemical synaptic transmission, postsynaptic / mossy fiber rosette / detection of cold stimulus involved in thermoception / Activation of Na-permeable kainate receptors / kainate selective glutamate receptor complex / Activation of Ca-permeable Kainate Receptor / negative regulation of synaptic transmission, glutamatergic / regulation of short-term neuronal synaptic plasticity ...regulation of synaptic vesicle fusion to presynaptic active zone membrane / protein retention in ER lumen / chemical synaptic transmission, postsynaptic / mossy fiber rosette / detection of cold stimulus involved in thermoception / Activation of Na-permeable kainate receptors / kainate selective glutamate receptor complex / Activation of Ca-permeable Kainate Receptor / negative regulation of synaptic transmission, glutamatergic / regulation of short-term neuronal synaptic plasticity / inhibitory postsynaptic potential / glutamate receptor activity / ubiquitin conjugating enzyme binding / receptor clustering / modulation of excitatory postsynaptic potential / regulation of JNK cascade / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / neuronal action potential / behavioral fear response / regulation of postsynaptic membrane potential / positive regulation of synaptic transmission / glutamate-gated receptor activity / glutamate-gated calcium ion channel activity / monoatomic ion transmembrane transport / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / presynaptic modulation of chemical synaptic transmission / dendrite cytoplasm / hippocampal mossy fiber to CA3 synapse / bioluminescence / regulation of membrane potential / SNARE binding / excitatory postsynaptic potential / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / generation of precursor metabolites and energy / synaptic transmission, glutamatergic / establishment of localization in cell / PDZ domain binding / cellular response to glucose stimulus / postsynaptic density membrane / regulation of long-term neuronal synaptic plasticity / modulation of chemical synaptic transmission / terminal bouton / SH3 domain binding / intracellular calcium ion homeostasis / positive regulation of neuron apoptotic process / presynaptic membrane / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / perikaryon / neuron apoptotic process / negative regulation of neuron apoptotic process / postsynaptic density / axon / neuronal cell body / glutamatergic synapse / ubiquitin protein ligase binding / synapse / dendrite / endoplasmic reticulum / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.5 Å | |||||||||
![]() | Khanra N / Brown PMGE / Perozzo AM / Bowie D / Meyerson JM | |||||||||
![]() | ![]() タイトル: Architecture and structural dynamics of the heteromeric GluK2/K5 kainate receptor. 著者: Nandish Khanra / Patricia Mge Brown / Amanda M Perozzo / Derek Bowie / Joel R Meyerson / ![]() ![]() 要旨: Kainate receptors (KARs) are L-glutamate-gated ion channels that regulate synaptic transmission and modulate neuronal circuits. KARs have strict assembly rules and primarily function as heteromeric ...Kainate receptors (KARs) are L-glutamate-gated ion channels that regulate synaptic transmission and modulate neuronal circuits. KARs have strict assembly rules and primarily function as heteromeric receptors in the brain. A longstanding question is how KAR heteromer subunits organize and coordinate together to fulfill their signature physiological roles. Here we report structures of the GluK2/GluK5 heteromer in apo, antagonist-bound, and desensitized states. The receptor assembles with two copies of each subunit, ligand binding domains arranged as two heterodimers and GluK5 subunits proximal to the channel. Strikingly, during desensitization, GluK2, but not GluK5, subunits undergo major structural rearrangements to facilitate channel closure. We show how the large conformational differences between antagonist-bound and desensitized states are mediated by the linkers connecting the pore helices to the ligand binding domains. This work presents the first KAR heteromer structure, reveals how its subunits are organized, and resolves how the heteromer can accommodate functionally distinct closed channel structures. | |||||||||
履歴 |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 259 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 11.9 KB 11.9 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 15 KB | 表示 | ![]() |
画像 | ![]() | 50.1 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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関連構造データ | ![]() 7ks0C ![]() 7ks3C C: 同じ文献を引用 ( |
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「今月の分子」の関連する項目 |
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ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | GluK2/K5 apo | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.083 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
-全体 : GluK2/K5 apo
全体 | 名称: GluK2/K5 apo |
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要素 |
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-超分子 #1: GluK2/K5 apo
超分子 | 名称: GluK2/K5 apo / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() ![]() |
組換発現 | 生物種: ![]() |
-分子 #1: Glutamate receptor ionotropic, kainate 5,Green fluorescent protei...
分子 | 名称: Glutamate receptor ionotropic, kainate 5,Green fluorescent protein chimera タイプ: protein_or_peptide / ID: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
組換発現 | 生物種: ![]() |
配列 | 文字列: MPAELLLLLI VAFANPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ INGIIEVPAK ARVEVDIFE LQRDSQYETT DTMCQILPKG VVSVLGPSSS PASASTVSHI CGEKEIPHIK V GPEETPRL QYLRFASVSL YPSNEDVSLA VSRILKSFNY PSASLICAKA ...文字列: MPAELLLLLI VAFANPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ INGIIEVPAK ARVEVDIFE LQRDSQYETT DTMCQILPKG VVSVLGPSSS PASASTVSHI CGEKEIPHIK V GPEETPRL QYLRFASVSL YPSNEDVSLA VSRILKSFNY PSASLICAKA ECLLRLEELV RG FLISKET LSVRMLDDSR DPTPLLKEIR DDKVSTIIID ANASISHLVL RKASELGMTS AFY KYILTT MDFPILHLDG IVEDSSNILG FSMFNTSHPF YPEFVRSLNM SWRENCEAST YPGP ALSAA LMFDAVHVVV SAVRELNRSQ EIGVKPLACT SANIWPHGTS LMNYLRMVEY DGLTG RVEF NSKGQRTNYT LRILEKSRQG HREIGVWYSN RTLAMNATTL DINLSQTLAN KTLVVT TIL ENPYVMRRPN FQALSGNERF EGFCVDMLRE LAELLRFRYR LRLVEDGLYG APEPNGS WT GMVGELINRK ADLAVAAFTI TAEREKVIDF SKPFMTLGIS ILYRVHMGRK PGYFSFLD P FSPAVWLFML LAYLAVSVVL FLAARLSPYE WYNPHPSLRA RPHILENQYT LGNSLWFPV GGFMQQGSEI MPRALSTRIV SGVWWAFTLI IISSYTANLA AFLTVQRMEV PVESADDLAD QTNIEYGTI HAGSTMTFFQ NSRYQTYQRM WNYMQSKQPS VFVKSTEEGI ARVLNSRYAF L LESTMNEY HRRLNCNLTQ IGGLLDTKGY GIGMPLGSPF RDEITLAILQ LQENNRLEIL KR KWWEGGR CPKEEDHRAK GLGMENIGGI FVVLIAGLII AVFVAVMEFI YKSRAEAKRM KGL VPRGSA AAAMVSKGEE LFTGVVPILV ELDGDVNGHK FSVSGEGEGD ATYGKLTLKF ICTT GKLPV PWPTLVTTLT YGVQCFSRYP DHMKQHDFFK SAMPEGYVQE RTIFFKDDGN YKTRA EVKF EGDTLVNRIE LKGIDFKEDG NILGHKLEYN YNSHNVYIMA DKQKNGIKVN FKIRHN IED GSVQLADHYQ QNTPIGDGPV LLPDNHYLST QSKLSKDPNE KRDHMVLLEF VTAAGIT LG MDELYKSGLR TETSQVAPA |
-分子 #2: Glutamate receptor ionotropic, kainate 2
分子 | 名称: Glutamate receptor ionotropic, kainate 2 / タイプ: protein_or_peptide / ID: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
組換発現 | 生物種: ![]() |
配列 | 文字列: MKIISPVLSN LVFSRSIKVL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK ...文字列: MKIISPVLSN LVFSRSIKVL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI RLVEDGKYGA QDDVNGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI LYRKPNGTNP GVFSFLNPLS PDIWMYVLLA YLGVSVVLFV IARFSPYEWY NPHPSNPDSD VVENNFTLLN SFWFGVGALM QQGSELMPKA LSTRIVGGIW WFFTLIIISS YTANLAAFLT VERMESPIDS ADDLAKQTKI EYGAVEDGAT MTFFKKSKIS TYDKMWAFMS SRRQSVLVKS NEEGIQRVLT SDYAFLMEST TIEFVTQRNC NLTQIGGLID SKGYGVGTPM GSPYRDKITI AILQLQEEGK LHMMKEKWWR GNGCPEEESK EASALGVQNI GGIFIVLAAG LVLSVFVAVG EFLYKSKKNA QLEKRSFCSA MVEELRMSLK CQRRLKHKPQ APVIVKTEEV INMHTFNDRR LPGKETMASG LRSAWSHPQF EKGGGSGGGS GGGSWSHPQF EK |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 3.7 mg/mL | |||||||||
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緩衝液 | pH: 8 構成要素:
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 295 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 51.23 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |