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- EMDB-22705: Cryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium... -

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Basic information

Entry
Database: EMDB / ID: EMD-22705
TitleCryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium tuberculosis with D8 symmetry
Map dataCryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium tuberculosis with D8 symmetry
Sample
  • Complex: mtTiME-FL (Rv3705c-FL)
Function / homologyPknH-like extracellular domain / PknH-like extracellular domain superfamily / PknH-like extracellular domain / Conserved protein
Function and homology information
Biological speciesMycobacterium tuberculosis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.4 Å
AuthorsCai X / He Y / Zhou ZH
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: Sci Adv / Year: 2021
Title: Identification and architecture of a putative secretion tube across mycobacterial outer envelope.
Authors: Xiaoying Cai / Lei Liu / Chunhong Qiu / Chongzheng Wen / Yao He / Yanxiang Cui / Siyu Li / Xuan Zhang / Longhua Zhang / Changlin Tian / Lijun Bi / Z Hong Zhou / Weimin Gong /
Abstract: Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion ...Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion system, but none has been reported. We show that Rv3705c and its homologous MSMEG_6251 in are tube-forming proteins in the mycobacterial envelope (TiME). Crystallographic and cryo-EM structures of these two proteins show that both proteins form rotationally symmetric rings. Two layers of TiME rings pack together in a tail-to-tail manner into a ring-shaped complex, which, in turn, stacks together to form tubes. TiME was detected mainly in the cell wall and capsule. Knocking out the TiME gene markedly decreased the amount of secreted protein in the culture medium, and expression of this gene in knocked-out strain partially restored the level of secreted protein. Our structure and functional data thus suggest that TiME forms a protein transport tube across the mycobacterial outer envelope.
History
DepositionSep 24, 2020-
Header (metadata) releaseSep 1, 2021-
Map releaseSep 1, 2021-
UpdateSep 1, 2021-
Current statusSep 1, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22705.png

Downloads & links

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Map

FileDownload / File: emd_22705.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium tuberculosis with D8 symmetry
Voxel sizeX=Y=Z: 1.07 Å
Density
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.02
Minimum - Maximum-0.05234936 - 0.12334127
Average (Standard dev.)0.0005263695 (±0.004601587)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 273.92 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z273.920273.920273.920
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.0520.1230.001

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Supplemental data

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Sample components

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Entire : mtTiME-FL (Rv3705c-FL)

EntireName: mtTiME-FL (Rv3705c-FL)
Components
  • Complex: mtTiME-FL (Rv3705c-FL)

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Supramolecule #1: mtTiME-FL (Rv3705c-FL)

SupramoleculeName: mtTiME-FL (Rv3705c-FL) / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Mycobacterium tuberculosis (bacteria)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 100.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 28290

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