- EMDB-22617: Cryo-EM structure of heterologous protein complex loaded Thermoto... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-22617
タイトル
Cryo-EM structure of heterologous protein complex loaded Thermotoga maritima encapsulin capsid
マップデータ
試料
細胞器官・細胞要素: Thermotoga maritima encapsulin
機能・相同性
Type 1 encapsulin shell protein / Encapsulating protein for peroxidase / encapsulin nanocompartment / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素 / peptidase activity / iron ion transport / intracellular iron ion homeostasis / proteolysis / Type 1 encapsulin shell protein
ジャーナル: Biomolecules / 年: 2020 タイトル: Cryo-EM Structure of Heterologous Protein Complex Loaded Encapsulin Capsid. 著者: Xiansong Xiong / Chen Sun / Frank S Vago / Thomas Klose / Jiankang Zhu / Wen Jiang / 要旨: Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM ...Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM structure of encapsulin with heterologous protein complex loaded. It is the first successful case of expressing encapsulin and heterologous cargo protein in the insect cell system. Although we failed to reconstruct the cargo protein complex structure due to the signal interference of the capsid shell, we were able to observe some unique features of the cargo-loaded encapsulin shell, for example, an extra density at the fivefold pore that has not been reported before. These results would lead to a more complete understanding of the encapsulin cargo assembly process of .