- EMDB-22524: Analysis of a strand exchange reaction with a mini filament of 9-... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-22524
タイトル
Analysis of a strand exchange reaction with a mini filament of 9-RecA, 27-mer ssDNA, partially-homologous 67 bp dsDNA and ATPgammaS.
マップデータ
consensus reconstruction postprocessed with relion
試料
複合体: Ensemble of products of a strand exchange reaction with partially-homologous 67 bp dsDNA and ATPgammaS
タンパク質・ペプチド: Protein RecA
DNA: DNA (27-MER)
DNA: DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')
リガンド: MAGNESIUM ION
リガンド: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
キーワード
Recombination / DNA repair / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
機能・相同性
機能・相同性情報
DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding ...DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding / DNA-binding transcription factor binding / DNA recombination / damaged DNA binding / DNA repair / DNA damage response / ATP hydrolysis activity / ATP binding / cytosol / cytoplasm 類似検索 - 分子機能
: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. ...: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
ジャーナル: Nature / 年: 2020 タイトル: Mechanism of strand exchange from RecA-DNA synaptic and D-loop structures. 著者: Haijuan Yang / Chun Zhou / Ankita Dhar / Nikola P Pavletich / 要旨: The strand-exchange reaction is central to homologous recombination. It is catalysed by the RecA family of ATPases, which form a helical filament with single-stranded DNA (ssDNA) and ATP. This ...The strand-exchange reaction is central to homologous recombination. It is catalysed by the RecA family of ATPases, which form a helical filament with single-stranded DNA (ssDNA) and ATP. This filament binds to a donor double-stranded DNA (dsDNA) to form synaptic filaments, which search for homology and then catalyse the exchange of the complementary strand, forming either a new heteroduplex or-if homology is limited-a D-loop. How synaptic filaments form, search for homology and catalyse strand exchange is poorly understood. Here we report the cryo-electron microscopy analysis of synaptic mini-filaments with both non-complementary and partially complementary dsDNA, and structures of RecA-D-loop complexes containing a 10- or a 12-base-pair heteroduplex. The C-terminal domain of RecA binds to dsDNA and directs it to the RecA L2 loop, which inserts into and opens up the duplex. The opening propagates through RecA sequestering the homologous strand at a secondary DNA-binding site, which frees the complementary strand to sample pairing with the ssDNA. At each RecA step, there is a roughly 20% probability that duplex opening will terminate and the as-yet-unopened dsDNA portion will bind to another C-terminal domain. Homology suppresses this process, through the cooperation of heteroduplex pairing with the binding of ssDNA to the secondary site, to extend dsDNA opening. This mechanism locally limits the length of ssDNA sampled for pairing if homology is not encountered, and could allow for the formation of multiple, widely separated synapses on the donor dsDNA, which would increase the likelihood of encountering homology. These findings provide key mechanistic insights into homologous recombination.
全体 : Ensemble of products of a strand exchange reaction with partially...
全体
名称: Ensemble of products of a strand exchange reaction with partially-homologous 67 bp dsDNA and ATPgammaS
要素
複合体: Ensemble of products of a strand exchange reaction with partially-homologous 67 bp dsDNA and ATPgammaS
タンパク質・ペプチド: Protein RecA
DNA: DNA (27-MER)
DNA: DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')
リガンド: MAGNESIUM ION
リガンド: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
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超分子 #1: Ensemble of products of a strand exchange reaction with partially...
超分子
名称: Ensemble of products of a strand exchange reaction with partially-homologous 67 bp dsDNA and ATPgammaS タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3