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- EMDB-22284: Head-Middle module of Mediator -

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Basic information

Entry
Database: EMDB / ID: EMD-22284
TitleHead-Middle module of Mediator
Map dataHead-Middle module of Mediator
Sample
  • Complex: Head-Middle module of Mediator
    • Protein or peptide: x 15 types
KeywordsTranscription
Function / homology
Function and homology information


core mediator complex / mediator complex / transcription coregulator activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II
Similarity search - Function
Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 ...Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med14 / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 9 / Uncharacterized protein / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 1 ...Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 9 / Uncharacterized protein / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 11
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus) / Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.2 Å
AuthorsZhang HQ / Chen DC
CitationJournal: Mol Cell / Year: 2021
Title: Mediator structure and conformation change.
Authors: Heqiao Zhang / Dong-Hua Chen / Rayees U H Mattoo / David A Bushnell / Yannan Wang / Chao Yuan / Lin Wang / Chunnian Wang / Ralph E Davis / Yan Nie / Roger D Kornberg /
Abstract: Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription ...Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription machinery. Previous structural studies revealed a relatively small part of Mediator and none of the gene activator-binding regions. We have determined the cryo-EM structure of the Mediator at near-atomic resolution. The structure reveals almost all amino acid residues in ordered regions, including the major targets of activator proteins, the Tail module, and the Med1 subunit of the Middle module. Comparison of Mediator structures with and without pol II reveals conformational changes that propagate across the entire Mediator, from Head to Tail, coupling activator- and pol II-interacting regions.
History
DepositionJul 8, 2020-
Header (metadata) releaseMar 3, 2021-
Map releaseMar 3, 2021-
UpdateMay 21, 2025-
Current statusMay 21, 2025Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.4
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.4
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6xp5
  • Surface level: 0.4
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22284.png

Downloads & links

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Map

FileDownload / File: emd_22284.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHead-Middle module of Mediator
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 480 pix.
= 526.08 Å		1.1 Å/pix.
x 480 pix.
= 526.08 Å		1.1 Å/pix.
x 480 pix.
= 526.08 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.096 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy EMDB: 0.17 / Movie #1: 0.4
Minimum - Maximum-0.40937865 - 1.271582
Average (Standard dev.)-0.00021797215 (±0.031755727)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 526.07996 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0961.0961.096
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z526.080526.080526.080
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.4091.272-0.000

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Supplemental data

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Sample components

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Entire : Head-Middle module of Mediator

EntireName: Head-Middle module of Mediator
Components
  • Complex: Head-Middle module of Mediator
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Med22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Unknown peptide
    • Protein or peptide: HEAT

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Supramolecule #1: Head-Middle module of Mediator

SupramoleculeName: Head-Middle module of Mediator / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Chaetomium thermophilum (fungus)

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Macromolecule #1: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.718674 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString:
DRLTQLQEAM DELLEMFLSA FFWVERHHDL KTFGPNDKIP DLKADQPKEV DTKSPEEYKA GQLQLARDII VKEQQIEYLI STLPGLDNS EREQIQLLRE LEDDLVTAEA QRQEALKERD EVLGKLDALL RGLERP

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

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Macromolecule #2: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 37.924664 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MDKELDGRFE RLEKALATMI DSLSKNNPST KLAQDLVAAE AELLEGLKLL EAHQNNHARI QQLRQSTEQA DAQIKDIISS LWKMRQELT SVQTSPIPKG AKFQFTTGEL LDFARRISRN TLPPPGVTNG VNMTPAAARH SQQPSSVEPE DSFRVTSQSQ T QTPNTSFN ...String:
MDKELDGRFE RLEKALATMI DSLSKNNPST KLAQDLVAAE AELLEGLKLL EAHQNNHARI QQLRQSTEQA DAQIKDIISS LWKMRQELT SVQTSPIPKG AKFQFTTGEL LDFARRISRN TLPPPGVTNG VNMTPAAARH SQQPSSVEPE DSFRVTSQSQ T QTPNTSFN VSFNGTLVDT PGPFNNNSTP IPNAGPANSQ NPHTELPEHL KLATNPLHGA SFFPWPSVEQ VRSGALGAYQ LL VDKGIDP RGYDPEFEEQ RRKDAEREAE ERAKQEREEA ERRAREEAER IARQRELERQ RARESMAAAA EEGRRDSVVG GPA AGKPKQ FTFLGGDDDD DDDDD

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

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Macromolecule #3: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 36.470812 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MADPSSSTSA MPLDEIQWHT NPAVVGGSIH DNSVLFYFRE SPFYDKTSNN EVLYQQGLHN QTMMQFLETR ERFERRLREM SGLEFVVAQ EPAETAPGTG TGVWVINKQT RRKRQGEEDE ISVHGTYFLV GENVYMAAPT LADIISMRLA SASSSISRLL P IAASVQSW ...String:
MADPSSSTSA MPLDEIQWHT NPAVVGGSIH DNSVLFYFRE SPFYDKTSNN EVLYQQGLHN QTMMQFLETR ERFERRLREM SGLEFVVAQ EPAETAPGTG TGVWVINKQT RRKRQGEEDE ISVHGTYFLV GENVYMAAPT LADIISMRLA SASSSISRLL P IAASVQSW SPGTGRVYKT PSMTSQTNQA NQPSSQTQSQ PTSQPATTTT TTALPPPDPR WLEETLMIHE FFGDHHLDKN PI TGKPGDF HLSSTGRKIQ TLPTAAAGNK KPGSGLPPLP AINTKVQQNP MGSGKQVTGK ETKSPKTPSS STGPGTGGMS TGM GKPKKR KSSKAAVTPT S

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

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Macromolecule #4: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 27.738227 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MDSSEDDGNK VAHLWPDPPA FWKDFTPENI ERYNALKQTY AQQHGLSSDA LVRIPDVPAD LINLQPPPEP VDGKWKLYGQ QEALANSLQ SLEDAGIQRL APASETTSDS KHFDRGFELK KLAKSLLVNY LELMGIMSIN PAHASEKVQD IKTIVLNFHH I LNEYRPHQ ...String:
MDSSEDDGNK VAHLWPDPPA FWKDFTPENI ERYNALKQTY AQQHGLSSDA LVRIPDVPAD LINLQPPPEP VDGKWKLYGQ QEALANSLQ SLEDAGIQRL APASETTSDS KHFDRGFELK KLAKSLLVNY LELMGIMSIN PAHASEKVQD IKTIVLNFHH I LNEYRPHQ AREQLIQLMQ DHLDAKRNET AAVRAVVDKA KRLIEGLASI EIPKVEDDNG RKAVEVERAA LAERREIAAW QE ADALFT

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

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Macromolecule #5: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 18.325607 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString:
MTAPLPEGLT PDAVDTLTEL SAIIKKLRAA QQQQAANPSS QAAAGGPGTT GAPGSQPSSS GPNATAAGGA IGTTSLPSSA TTGPTPSNA LHSVKELPAT TDPIKHKLQR ARAAVRLLGD MSRTMAQQEA ELARLEERRR KQAAMLAKAQ EEGARLSKGF G EVGETPVV LVETGDKMAM E

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

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Macromolecule #6: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 133.450359 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MEARAHGALP NNYDRERFIN GVGGDDKALK RKLEEPLSDI TKELDTVIAQ SDVAVADPKG QLCPDADVPD EMEHITDGIL PLNLLLIRL AEFSHSKLEE LVTMLASKPV PQHAVNGNGS HSTLVEDASP ESQEKKRLLL NTIQDLHSRW VKALVITEWA R NAEKVGKL ...String:
MEARAHGALP NNYDRERFIN GVGGDDKALK RKLEEPLSDI TKELDTVIAQ SDVAVADPKG QLCPDADVPD EMEHITDGIL PLNLLLIRL AEFSHSKLEE LVTMLASKPV PQHAVNGNGS HSTLVEDASP ESQEKKRLLL NTIQDLHSRW VKALVITEWA R NAEKVGKL IDIRTHLFKK LELYPQVLND FINLKRDMAW AKVPSPDLKT ALHILTHGEV TWMPDFNFLD PPPLTTEETL RW INDMNLA LHARLQLEEH DKLPPPFKNY TIDSGRVTFR VRGEFEVDLS ISEEDFSEQF WFINFRFDFS PAPAELTPAV RYW MTEKVN NILKTEGLGG CYKYLHEFTL TQKIAELHRQ AIALNKGRWA NSLRVEKLNR NLGIHYWANR LHSQNLKSWI IIGV HSGED PQGLEEPKPS YLMVQWFREG VEQFIDIPFS QEKLSIEEIL EFVTAKHVGY LLFSLFRKFD GKPRFTQGKK ARLEL NVWT QKPEDYYLSM QLLDDEDLKI QVNPWMGDFI TTPSTGPWNR TLNSLGNPAE EGSKELENFR YIYIISVLKA QGKSHG WSV VRSPISQDEL RSIVHSESAS SRETFQAAWT RFVDWDPQWY AMRSMSLAGD QWWLVEVTSQ RQSISGNRLV FFTKMAP CF SEERLTDGFF NGLRDHSRRL IAEITNLREL YPGITPSQLR KLIDPTRQPP VLPVKASKIL GDFGGADAQS IAWLKDPV W LIYRGSACDA AATRVSDSPQ QKRTQPVLDV FEAYLDVTDR RRFQTLNPRL DRDILYNPYT GRFMFRLRAK MGVPVVPLL GIRVRQLQRL LDFLEGLRRV GDQAVPERVT LREIVFSYGA TRKRDSTNQP KVRPWRVRLD LTKEEGVGVV LEKGNPHLRV INRLEDLVN SQKFYSLATY LTLSLPLFRA FEQLENAWQT AQENGRGSCF ILHLSLDKHT IRFVLPGHQR QISLLIQPHE K EGKLVWEV GRPRHDPELL NENCEFNRVL KERVWTISGS GFKGLVTGAA AEPDEGIERL LVLISDAVLH LSTSQPATAP PQ PPQAQPS QQVQQVQQQQ VVSQLQPQAQ QPRSAMPHAS VGQQQPHGAP APMARFPPQQ QQIFQQQRPP QPQPNMHGGQ ISQ PNMHGG PKPQLQGQHG QPGQMVAAPQ QQGQRPGTGT AAGMGRSNAP LVVLD

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

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Macromolecule #7: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 69.61693 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MTDRPFILQV PPTQQRGPQN IAEFVARINP DAFRVLNEAE LRRKVEEEKN GVGQDEDVDM ESSPSDNEGE PADAKDIITA KHEMLRVID QTRQTTLYAL DFVSLLLSKE NPGQAVTTFS PGLRELVGIG TLGATMLDAP TPLTQSRIPD NKLVTIGKRL M DLNKAADT ...String:
MTDRPFILQV PPTQQRGPQN IAEFVARINP DAFRVLNEAE LRRKVEEEKN GVGQDEDVDM ESSPSDNEGE PADAKDIITA KHEMLRVID QTRQTTLYAL DFVSLLLSKE NPGQAVTTFS PGLRELVGIG TLGATMLDAP TPLTQSRIPD NKLVTIGKRL M DLNKAADT ALAASKRLQK EIAAETKYWS EVLAVRNDGW QTSRMPREPQ TMGVKFGFNN AAPEFKAVSI APMRRADNGS VL LDRSSIG HKSQRIQVRI LEDGRIVGRS SLPSPVSADA PLQDRVKEMR DTIFAQELWH EINREARTLL NQGVHLEPSS ITY TMDAST TVSIRLATLG EEEEGLDEQQ EGPQDVMAES LNIALSLLLS HAHRMNELRR SEPGINKGPP RTYPILLPLI SYHK YNQSI QTCLQTLSAH ISVLRSASVD SSMTVKEPLL SSPPGAPAAT SLYTILTRPP AVQFDITITP DSRIRILLKP TQLTG AAFS ICCLPALHPG AQNPLATTCP PSTDDYDSLT QVVSYLQAAI PRALAAHYEA VLVVEATVNG GMLGDLPPSP SRWMPL IDG KGIVDPETMR FGIRFSFGRN PAKGGQLELG AQTDYVDGSR KRVRRNWIWP GAQSSLDSVA KHVLARGPPE E

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

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Macromolecule #8: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 14.486557 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString:
MSVDSSGMEP PPLPSDGPEE PKHGGHTRFE LELEFVQALS NPEYVNWLAS RKYLMNPAFV AYLDYLQYWS RPPYLKYLTY PTATLRMLQ LLQSERFRQE ILVPDVAWQL RLEGMKAAVL WHKEG

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

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Macromolecule #9: Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 32.098467 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MASLNLAPEE LKQLELLRNR FAQLQSSLTS LAGNLIRTEP LPTYESLQAS ASILQQNLRS IQELMTENSD IFKRIAIHPS TNFPGRTQE HMLLQLLRKK LEPEVESWVE EARETARAAG IDVSKLSGPG GGAPTRGMNG YGDDDEYGLD DEDEGVPSDP F NEQWADML ...String:
MASLNLAPEE LKQLELLRNR FAQLQSSLTS LAGNLIRTEP LPTYESLQAS ASILQQNLRS IQELMTENSD IFKRIAIHPS TNFPGRTQE HMLLQLLRKK LEPEVESWVE EARETARAAG IDVSKLSGPG GGAPTRGMNG YGDDDEYGLD DEDEGVPSDP F NEQWADML ETFQHSLHHY VTVQLKKQYT VEEQEMGIEN VRTGLKRELG DEDEDEDEDE EEEEEGVAGV AGGAASQGAG AG TAGAAAK KPVIQPEYLF WLAARGDTRV SRNIEFEAMR KVAQTAKRPA PPR

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

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Macromolecule #10: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 25.50427 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MAQPSQSSVP ESDAGSFPVD IHKPFTPAER IQQLGEIDND IASLLQHLSA ALKALATPPG KRIFQEQDSE SNSPSASDTS NPNDTPPSS CGIDPVTAFK TAQNDFFRTI DRIDKHLTRQ IYALEEAGII TLKSGTGSGA GTVGAGATEE QAQQLPQPQM I PGQPQAAG ...String:
MAQPSQSSVP ESDAGSFPVD IHKPFTPAER IQQLGEIDND IASLLQHLSA ALKALATPPG KRIFQEQDSE SNSPSASDTS NPNDTPPSS CGIDPVTAFK TAQNDFFRTI DRIDKHLTRQ IYALEEAGII TLKSGTGSGA GTVGAGATEE QAQQLPQPQM I PGQPQAAG ITADASVAPV PKARLEPDGM GRYGKLDVGR LNMASSIVER EMEGELWRKA REHLGRMTSQ ASGREDRMEE

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

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Macromolecule #11: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 38.72575 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MPYEIFLAGV VSDNDEAKAR AVLGGFTEMR EHHRFTRVQH YEPQDIKIRG FPTIKELLKE RGPTTALYQE LHNILVRQPY TLQIRTNIT DAILGPAQAG AEAAPHGTVS GGKPHILRWS DIPDPPNQRI PPFITQRKII EIPDQRAPRI LAASKFRNKS D LVEESYHW ...String:
MPYEIFLAGV VSDNDEAKAR AVLGGFTEMR EHHRFTRVQH YEPQDIKIRG FPTIKELLKE RGPTTALYQE LHNILVRQPY TLQIRTNIT DAILGPAQAG AEAAPHGTVS GGKPHILRWS DIPDPPNQRI PPFITQRKII EIPDQRAPRI LAASKFRNKS D LVEESYHW WFNDVEYSMV RTYLISLDPS PQTTDQVPSL AGLEPIGDFW LLYVRMRVEA TPDRMQQAHN QLQQIRDQLL GV FEFKVWD RRAHDTRIME QPTQGNGNVI GDAHNSNKKS NGVSPTTASS SEEVSPVDSQ LTIPLPDDDD GDNVEDTPDT EMD EDDDES EYDSDSERDW PKDD

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

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Macromolecule #12: Med22

MacromoleculeName: Med22 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 18.341615 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString:
MDRDQGASDN LLERKNILIA SIMTSYRDLI THATSPITSA TASPGHAGYS SMALSTAIHA AVKYTEDLLS LTRTLREALW VVGPLTGPG EKDAQAEEGM AKDAEVVWDV LNEMRDRERE RMMAALMEGD TRVRGAVRFE RGDVEVVVAG QGQQIEMGRG V NGEVKSEG A

UniProtKB: Uncharacterized protein

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Macromolecule #13: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 81.633266 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: MSTPIPTPGR NPLQGRTPSQ AHGAAGTPPV STPFSAAQAV FSPNGPRSSP QQFKRSPATV GSTTATGGRP PSAINFDSPS ASAALGALQ PMTPGMDMTI RSLANLGRAS EDERAKRLEE VIHILSKSKG LVSEAGLERL ARSLELEFMW ESSMGSDNKR T LIVAGSAL ...String:
MSTPIPTPGR NPLQGRTPSQ AHGAAGTPPV STPFSAAQAV FSPNGPRSSP QQFKRSPATV GSTTATGGRP PSAINFDSPS ASAALGALQ PMTPGMDMTI RSLANLGRAS EDERAKRLEE VIHILSKSKG LVSEAGLERL ARSLELEFMW ESSMGSDNKR T LIVAGSAL ELLVEFSQDV VQSVTLNFPD SPEIVTKHAQ KAGEILFNDL RLAPGQSPLT KSLARFAANF ERLAVLDKLS IL PGLNLYE AVADVYESLA RLHAWELQKL REDPSLAGKD DEYLENLVLC TKSGKPAMND RGRVGLALDY WKEKHLLRTP EAE VASWIA DNERTWSILI GCAPLRDLSI NPVRISDKWI GPNVVKTSLP DGLHTGEPII DWLDPEPTFI PATDQQQQQQ QQQQ QQQQQ QQQQQQADAP LLPPRLPEVA FHAVFDPPVH IPINLWQHLQ EMGCVLPDET ATQPFQQHHF RSFDSLVIPP PQGKE TDAT ETRVFSCRKK TPFVPRRSGV RNGEGATQKM EYRIHQNTLF VPKPVMCRTL TEMSFSHPQQ LVSILPYLRQ YAFLAQ LLE NSFKPGPALQ LPTDPNADTT TSKPTPNAKA SHKKLDDFEA FLAKHSSHSG PISNMTPSQH QPSSDLSIDV TLRLVLP SP KLQIVFPLHA TSKEEVKGKI AHVTFAVREN GRLEVEMQNM IEEEGDQMEG VKTEGDEDGE EKKRRRPQPG DLARALEV L EDVGKWAEFV RTRWV

UniProtKB: Mediator of RNA polymerase II transcription subunit 1

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Macromolecule #14: Unknown peptide

MacromoleculeName: Unknown peptide / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 1.805216 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #15: HEAT

MacromoleculeName: HEAT / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus)
Molecular weightTheoretical: 17.038955 KDa
Recombinant expressionOrganism: Chaetomium thermophilum (fungus)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5n9j

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 108348
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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