ジャーナル: Apidologie / 年: 2022 タイトル: Nuclear translocation of vitellogenin in the honey bee (). 著者: Heli Salmela / Gyan P Harwood / Daniel Münch / Christine G Elsik / Elías Herrero-Galán / Maria K Vartiainen / Gro V Amdam / 要旨: Vitellogenin (Vg) is a conserved protein used by nearly all oviparous animals to produce eggs. It is also pleiotropic and performs functions in oxidative stress resistance, immunity, and, in honey ...Vitellogenin (Vg) is a conserved protein used by nearly all oviparous animals to produce eggs. It is also pleiotropic and performs functions in oxidative stress resistance, immunity, and, in honey bees, behavioral development of the worker caste. It has remained enigmatic how Vg affects multiple traits. Here, we asked whether Vg enters the nucleus and acts via DNA-binding. We used cell fractionation, immunohistology, and cell culture to show that a structural subunit of honey bee Vg translocates into cell nuclei. We then demonstrated Vg-DNA binding theoretically and empirically with prediction software and chromatin immunoprecipitation with sequencing (ChIP-seq), finding binding sites at genes influencing immunity and behavior. Finally, we investigated the immunological and enzymatic conditions affecting Vg cleavage and nuclear translocation and constructed a 3D structural model. Our data are the first to show Vg in the nucleus and suggest a new fundamental regulatory role for this ubiquitous protein. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13592-022-00914-9.
pH: 7.5 / 構成要素 - 濃度: 150.0 mM / 構成要素 - 式: NaCl / 構成要素 - 名称: sodium chloride / 詳細: 20 mM Tris
染色
タイプ: NEGATIVE / 材質: Uranyl Acetate 詳細: Aliquotes of pure Vg (1.1 mg/ml in 20 mM Tris, 150 mM NaCl, pH 7.5) were applied to carbon-coated copper grids (30 s) and stained with 2% uranyl acetate
グリッド
材質: COPPER / 前処理 - タイプ: GLOW DISCHARGE
-
電子顕微鏡法
顕微鏡
JEOL 1230
撮影
フィルム・検出器のモデル: TVIPS TEMCAM-F415 (4k x 4k) 平均電子線量: 25.0 e/Å2