- EMDB-20830: Cryo-EM reconstruction of the inner membrane rings containing the... -
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Open data
ID or keywords:
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Basic information
Entry
Database: EMDB / ID: EMD-20830
Title
Cryo-EM reconstruction of the inner membrane rings containing the core components of the export apparatus and associated membranes of the needle complex from Salmonella typhimurium type III secretion system.
Map data
Sample
Complex: PrgH and PrgK, the protein components of the inner rings of the Salmonella's needle complex, in complex with the core components of the export apparatus and associated membranes.
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2019 Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway. Authors: Carmen Butan / Maria Lara-Tejero / Wenwei Li / Jun Liu / Jorge E Galán / Abstract: Type III protein secretion systems are essential virulence factors for many important pathogenic bacteria. The entire protein secretion machine is composed of several substructures that organize into ...Type III protein secretion systems are essential virulence factors for many important pathogenic bacteria. The entire protein secretion machine is composed of several substructures that organize into a holostructure or injectisome. The core component of the injectisome is the needle complex, which houses the export apparatus that serves as a gate for the passage of the secreted proteins through the bacterial inner membrane. Here, we describe a high-resolution structure of the export apparatus of the type III secretion system in association with the needle complex and the underlying bacterial membrane, both in isolation and in situ. We show the precise location of the core export apparatus components within the injectisome and bacterial envelope and demonstrate that their deployment results in major membrane remodeling and thinning, which may be central for the protein translocation process. We also show that InvA, a critical export apparatus component, forms a multiring cytoplasmic conduit that provides a pathway for the type III secretion substrates to reach the entrance of the export gate. Combined with structure-guided mutagenesis, our studies provide major insight into potential mechanisms of protein translocation and injectisome assembly.
History
Deposition
Oct 15, 2019
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Header (metadata) release
Nov 13, 2019
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Map release
Jul 1, 2020
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Update
Jul 1, 2020
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Current status
Jul 1, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_20830.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel size
X=Y=Z: 1.32 Å
Density
Contour Level
By AUTHOR: 0.0064 / Movie #1: 0.0064
Minimum - Maximum
-0.026692681 - 0.047679365
Average (Standard dev.)
0.00032297813 (±0.0030752753)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
280
280
280
Spacing
280
280
280
Cell
A=B=C: 369.6 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.32
1.32
1.32
M x/y/z
280
280
280
origin x/y/z
0.000
0.000
0.000
length x/y/z
369.600
369.600
369.600
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
280
280
280
D min/max/mean
-0.027
0.048
0.000
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Supplemental data
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Sample components
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Entire : PrgH and PrgK, the protein components of the inner rings of the S...
Entire
Name: PrgH and PrgK, the protein components of the inner rings of the Salmonella's needle complex, in complex with the core components of the export apparatus and associated membranes.
Components
Complex: PrgH and PrgK, the protein components of the inner rings of the Salmonella's needle complex, in complex with the core components of the export apparatus and associated membranes.
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Supramolecule #1: PrgH and PrgK, the protein components of the inner rings of the S...
Supramolecule
Name: PrgH and PrgK, the protein components of the inner rings of the Salmonella's needle complex, in complex with the core components of the export apparatus and associated membranes. type: complex / ID: 1 / Parent: 0
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