National Natural Science Foundation of China (NSFC)
31672489
China
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM071940
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI094386
United States
National Institutes of Health/Office of the Director
1S10OD018111
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
1S10RR23057
United States
National Science Foundation (NSF, United States)
DMR-1548924
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM116792
United States
National Science Foundation (NSF, United States)
DBI-1338135
United States
Citation
Journal: Nat Struct Mol Biol / Year: 2019 Title: Conservative transcription in three steps visualized in a double-stranded RNA virus. Authors: Yanxiang Cui / Yinong Zhang / Kang Zhou / Jingchen Sun / Z Hong Zhou / Abstract: Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV) ...Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 Å) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses.
History
Deposition
Aug 13, 2019
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Header (metadata) release
Sep 18, 2019
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Map release
Nov 20, 2019
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Update
Dec 25, 2019
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Current status
Dec 25, 2019
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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