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Yorodumi- EMDB-20098: Cryo-EM reconstruction of the '0110' binding pattern in the ADP-P... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-20098 | |||||||||
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Title | Cryo-EM reconstruction of the '0110' binding pattern in the ADP-Pi condition for dimeric kinesin-1 with two central bound sites and empty flanking sites | |||||||||
Map data | None | |||||||||
Sample |
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Biological species | homo s (cattle) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.55 Å | |||||||||
Authors | Cha HK / Debs G | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structural Intermediates of the Dimeric Kinesin Stepping Cycle Revealed by Cryo-EM Authors: Cha HK / Debs G / Liu X / Liu D / Sindelar CV | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_20098.map.gz | 126.4 MB | EMDB map data format | |
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Header (meta data) | emd-20098-v30.xml emd-20098.xml | 9 KB 9 KB | Display Display | EMDB header |
Images | emd_20098.png | 135.9 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-20098 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-20098 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_20098.map.gz / Format: CCP4 / Size: 160.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | None | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.715 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Dimeric kinesin-1 in the presence of 0.2uM ADP and 10mM Pi bound ...
Entire | Name: Dimeric kinesin-1 in the presence of 0.2uM ADP and 10mM Pi bound to taxol-stabilized microtubules |
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Components |
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-Supramolecule #1: Dimeric kinesin-1 in the presence of 0.2uM ADP and 10mM Pi bound ...
Supramolecule | Name: Dimeric kinesin-1 in the presence of 0.2uM ADP and 10mM Pi bound to taxol-stabilized microtubules type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: homo s (cattle) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.5 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Initial angle assignment | Type: PROJECTION MATCHING |
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Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 7.55 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 3167 |