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- EMDB-19519: IFTB1 (distal region) in retrograde Intraflagellar transport trains -
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Open data
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Basic information
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Title | IFTB1 (distal region) in retrograde Intraflagellar transport trains | |||||||||
![]() | Overall unfiltered map | |||||||||
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![]() | Cilia / IFT / train / retrograde / PROTEIN TRANSPORT | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 28.28 Å | |||||||||
![]() | Lacey SE / Pigino G | |||||||||
Funding support | European Union, 2 items
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![]() | ![]() Title: Retrograde intraflagellar transport trains are a unique polymeric arrangement of IFT complexes Authors: Lacey SE / Graziadei A / Pigino G | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 7.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14 KB 14 KB | Display Display | ![]() |
Images | ![]() | 20.2 KB | ||
Masks | ![]() | 10.5 MB | ![]() | |
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() ![]() | 57.8 KB 7.9 MB 7.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 827.4 KB | Display | ![]() |
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Full document | ![]() | 827 KB | Display | |
Data in XML | ![]() | 9.2 KB | Display | |
Data in CIF | ![]() | 10.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Overall unfiltered map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 6.06 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: The main map after Locspiral filtering and masking....
File | emd_19519_additional_1.map | ||||||||||||
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Annotation | The main map after Locspiral filtering and masking. This was then used for composite map generation | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_19519_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_19519_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : In situ retrograde intraflagellar transport trains in dhc1b-3 mut...
Entire | Name: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia |
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Components |
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-Supramolecule #1: In situ retrograde intraflagellar transport trains in dhc1b-3 mut...
Supramolecule | Name: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia type: cell / ID: 1 / Parent: 0 Details: Temperature sensitive cells incubated at restrictive temperature (34 degrees C) for 10 hours and plunge frozen on grids. |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 2.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 28.28 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1.3) / Number subtomograms used: 3177 |
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Extraction | Number tomograms: 756 / Number images used: 10455 |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |