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- EMDB-19515: Structure of IFTA and IFTB in Retrograde Intraflagellar transport... -

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Basic information

Entry
Database: EMDB / ID: EMD-19515
TitleStructure of IFTA and IFTB in Retrograde Intraflagellar transport trains
Map data
Sample
  • Cell: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
    • Protein or peptide: x 20 types
KeywordsCilia / IFT / train / retrograde / PROTEIN TRANSPORT
Function / homology
Function and homology information


intraciliary transport particle / intraciliary transport particle B binding / intraciliary transport involved in cilium assembly / intraciliary transport particle B / intraciliary transport particle A / cellular component assembly / outer dynein arm assembly / cilium-dependent cell motility / intraciliary retrograde transport / intraciliary transport ...intraciliary transport particle / intraciliary transport particle B binding / intraciliary transport involved in cilium assembly / intraciliary transport particle B / intraciliary transport particle A / cellular component assembly / outer dynein arm assembly / cilium-dependent cell motility / intraciliary retrograde transport / intraciliary transport / regulation of cilium assembly / axoneme assembly / axonemal microtubule / cilium organization / protein localization to cilium / non-motile cilium assembly / ciliary plasm / non-motile cilium / motile cilium / ciliary membrane / post-transcriptional regulation of gene expression / ciliary base / microtubule organizing center / axoneme / cilium assembly / kinesin binding / RNA processing / beta-tubulin binding / centriole / tubulin binding / regulation of microtubule cytoskeleton organization / regulation of protein stability / apical part of cell / protein transport / microtubule binding / cytoskeleton / protein stabilization / cilium / ciliary basal body / GTPase activity / GTP binding / metal ion binding / cytoplasm
Similarity search - Function
Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport protein 20 / Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport complex B, subunit 20 / Intraflagellar transport complex B protein 46 / Intraflagellar transport protein 74 / Tetratricopeptide repeat protein 30 / : ...Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport protein 20 / Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport complex B, subunit 20 / Intraflagellar transport complex B protein 46 / Intraflagellar transport protein 74 / Tetratricopeptide repeat protein 30 / : / : / : / Intraflagellar transport complex B protein 46 C terminal / IFT80 second beta-propeller / IFT52 central domain / IFT52 GIFT domain / Intraflagellar transport protein 25 / Intraflagellar transport protein 81 / IFT81, calponin homology domain / IFT81, N-terminal domain superfamily / Intraflagellar transport 81 calponin homology domain / Intraflagellar transport protein 52 homolog / TRAF3-interacting protein 1, N-terminal / TRAF3-interacting protein 1, C-terminal domain / TRAF3-interacting protein 1, N-terminal domain superfamily / : / Microtubule-binding protein MIP-T3 CH-like domain / Microtubule-binding protein MIP-T3 C-terminal region / Intraflagellar transport protein 52, C-terminal domain / TRAF3-interacting protein 1 / : / IFT122, zinc ribbon domain / Tetratricopeptide repeat protein 21A/21B / : / : / : / : / : / Tetratricopeptide repeat protein 21 ARM repeat / Tetratricopeptide repeat protein 21 second ARM domain / Tetratricopeptide repeat protein 21 N-terminal ARM repeat / Tetratricopeptide repeat protein 21 C-terminal ARM domain / TT21 fifth ARM repeats domain / Tetratricopeptide repeat protein 21 forth ARM domain / Tetratricopeptide repeat / WD repeat protein 35 / WDR19, WD40 repeat / WD repeat-containing protein 19/dyf-2 / : / : / : / : / : / WDR19 second beta-propeller / IFT140 first beta-propeller / IFT140 second beta-propeller / WDR19 first beta-propeller / WDR35 second beta-propeller / IF140 C-terminal TPR domain / WDR35/TULP4 N-terminal / WD repeat-containing protein 35, TPR repeats / : / : / IFT121, second zinc finger domain / IF140/IFT172 TPR domain / : / IFT80/172/WDR35/WDR19 TPR domain / Intraflagellar transport protein 122 homolog / : / : / IFT122 second beta-propeller / IFT122 first beta-propeller / IFT122, TPR domain / Ras of Complex, Roc, domain of DAPkinase / Quinoprotein amine dehydrogenase, beta chain-like / Tetratricopeptide repeat / Small GTPase Rab domain profile. / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Quinoprotein alcohol dehydrogenase-like superfamily / Tetratricopeptide repeat / Class I glutamine amidotransferase-like / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Galactose-binding-like domain superfamily / Rab subfamily of small GTPases / Small GTP-binding protein domain / Tetratricopeptide-like helical domain superfamily / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Uncharacterized protein / Uncharacterized protein / Intraflagellar transport protein 46 / Intraflagellar transport protein 80 / Intraflagellar transport protein 22 / Intraflagellar transport protein 27 / Uncharacterized protein / TRAF3-interacting protein 1 / Uncharacterized protein / Intraflagellar transport protein 121 ...Uncharacterized protein / Uncharacterized protein / Intraflagellar transport protein 46 / Intraflagellar transport protein 80 / Intraflagellar transport protein 22 / Intraflagellar transport protein 27 / Uncharacterized protein / TRAF3-interacting protein 1 / Uncharacterized protein / Intraflagellar transport protein 121 / Intraflagellar transport protein 139 / Intraflagellar transport protein 144 / Intraflagellar transport protein 25 / Intraflagellar transport protein 122 homolog / Intraflagellar transport protein 57 / Intraflagellar transport protein 172 / Intraflagellar transport protein 81 / Intraflagellar transport protein 74 / Intraflagellar transport particle protein IFT20 / Osm-6-like protein
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsubtomogram averaging / cryo EM / Resolution: 15.4 Å
AuthorsLacey S / Pigino G
Funding supportEuropean Union, 2 items
OrganizationGrant numberCountry
European Research Council (ERC)European Union
Other government
CitationJournal: Cell / Year: 2024
Title: Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport.
Authors: Samuel E Lacey / Andrea Graziadei / Gaia Pigino /
Abstract: Bidirectional transport in cilia is carried out by polymers of the IFTA and IFTB protein complexes, called anterograde and retrograde intraflagellar transport (IFT) trains. Anterograde trains deliver ...Bidirectional transport in cilia is carried out by polymers of the IFTA and IFTB protein complexes, called anterograde and retrograde intraflagellar transport (IFT) trains. Anterograde trains deliver cargoes from the cell to the cilium tip, then convert into retrograde trains for cargo export. We set out to understand how the IFT complexes can perform these two directly opposing roles before and after conversion. We use cryoelectron tomography and in situ cross-linking mass spectrometry to determine the structure of retrograde IFT trains and compare it with the known structure of anterograde trains. The retrograde train is a 2-fold symmetric polymer organized around a central thread of IFTA complexes. We conclude that anterograde-to-retrograde remodeling involves global rearrangements of the IFTA/B complexes and requires complete disassembly of the anterograde train. Finally, we describe how conformational changes to cargo-binding sites facilitate unidirectional cargo transport in a bidirectional system.
History
DepositionJan 31, 2024-
Header (metadata) releaseJul 24, 2024-
Map releaseJul 24, 2024-
UpdateAug 6, 2025-
Current statusAug 6, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19515.png

Downloads & links

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Map

FileDownload / File: emd_19515.map.gz / Format: CCP4 / Size: 66 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
6.06 Å/pix.
x 224 pix.
= 1357.44 Å		6.06 Å/pix.
x 345 pix.
= 2090.7 Å		6.06 Å/pix.
x 224 pix.
= 1357.44 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 6.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.5
Minimum - Maximum0.0 - 11.328854
Average (Standard dev.)0.04508458 (±0.47787383)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-30-68-53
Dimensions345224224
Spacing224345224
CellA: 1357.44 Å / B: 2090.7 Å / C: 1357.44 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : In situ retrograde intraflagellar transport trains in dhc1b-3 mut...

EntireName: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
Components
  • Cell: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
    • Protein or peptide: Intraflagellar transport protein 144
    • Protein or peptide: Intraflagellar transport particle protein 140
    • Protein or peptide: Intraflagellar transport protein 122 homolog
    • Protein or peptide: Intraflagellar transport protein 121
    • Protein or peptide: Intraflagellar transport protein 139
    • Protein or peptide: IFT88
    • Protein or peptide: IFT70
    • Protein or peptide: Osm-6-like protein
    • Protein or peptide: Intraflagellar transport protein 172
    • Protein or peptide: Intraflagellar transport protein 80
    • Protein or peptide: IFT54
    • Protein or peptide: Intraflagellar transport particle protein IFT20
    • Protein or peptide: Intraflagellar transport protein 57
    • Protein or peptide: Clusterin-associated protein 1
    • Protein or peptide: Intraflagellar transport protein 74
    • Protein or peptide: Intraflagellar transport protein 81
    • Protein or peptide: Intraflagellar transport protein 27
    • Protein or peptide: Intraflagellar transport protein 25
    • Protein or peptide: Intraflagellar transport protein 46
    • Protein or peptide: Intraflagellar transport protein 22

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Supramolecule #1: In situ retrograde intraflagellar transport trains in dhc1b-3 mut...

SupramoleculeName: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
type: cell / ID: 1 / Parent: 0
Macromolecule list: #1-#2, #15-#19, #3, #14, #4-#6, #20, #7-#13
Details: Temperature sensitive cells incubated at restrictive temperature (34 degrees C) for 10 hours and plunge frozen on grids.
Source (natural)Organism: Chlamydomonas reinhardtii (plant) / Strain: dhc1b-3

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Macromolecule #1: Intraflagellar transport protein 144

MacromoleculeName: Intraflagellar transport protein 144 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 150.972844 KDa
SequenceString: MKKLFGVGPD LLGDGKVLFE WSPKGNFLAA AGSKRKVNIF DRNGRLYDEV HFPPAEYPNP DGRACAAAQM QWDPAGEQLA ILPAGNTHV FIWLAGNKEV QKIESEFKTQ EFSCMAWSRN GMYLGVATVK GNVMMYNARE RKKTPLVGKH TKKIVAAAWN K DNIMALAG ...String:
MKKLFGVGPD LLGDGKVLFE WSPKGNFLAA AGSKRKVNIF DRNGRLYDEV HFPPAEYPNP DGRACAAAQM QWDPAGEQLA ILPAGNTHV FIWLAGNKEV QKIESEFKTQ EFSCMAWSRN GMYLGVATVK GNVMMYNARE RKKTPLVGKH TKKIVAAAWN K DNIMALAG QDKTVTLTDG VTGDTIKTFH LKDVPMDLCV SDKKEDGYSR REENTYSLNI NRKTLYIMQC TAEGDRPLEL AF LDTYGPI MKHSWFGDGY ILLGYKNGYV AVVSSHSREI SEEVHSGKYL DTLTDVTYCA SLGRVAMAGA NCVRVLDANA DYN EIKGDA VDLDANQAIE KVGWTKDGQV LTVGTHNGYM HSFLASLPMV YDFHGTRVLY LTSLLEMTLL DVSRRQTVAR IELE NEPAF CGLGPSHAAV GMNNQAAFYS LGEKVGKVVQ RREYLGTITA IKLNETQAAV LTGGHVVVHP ISVEAGHAPD ELDVV IPGP GQPANITCVA LTPTFVITGS RTGTLSYYLS PDVTPVNEFR HDDGGIVRLF PQATGARLVF EDDKGALHLF NPVNDH VVA VPYTGRAETV MWDTSDTNVM VIGDGTALHS FLYVPVSLTG PQVQDLGKQA VPATHTPLTV CNGVVGCRLK SGAMDNV TL ESHKMLQPGD AVARAAPAKR FAAALKLYKL RDAVECAKQL RQVESWRTLA LAALDVLDID TAINAYREIG DASMVLSL E RVRQHEDRNL LSAHIMVLLE KDYGQAQELF LRSSVPRAAL EMRMDLKHWT DALKLAEQLD PDAIATICKE HGAMLEMTG EYSNAKSHYQ QALDALAVSV GPAQPDLEAA CKAGIARTTL QLGDLRQGRQ LAMQLNSQTL FKECALILEG LQQLTEAAEM YERAGQFER AASIYIQTKN FAAAAPLMAR ISSSKLQLQF AKAKEAEGRW QEAAAAYEAA GDMDAVVRLC LERLSQPQRA Y AIVRKTQS VEAANQLSRF CLQSQDFGGA VEFLLMAGQM DQAFDIAMGH NEMDTFARIV AASAKPVDYQ RIAQYYESRG EY DKAADMW SKCDQAPRAV QLYLKVGTNP ALEKAVQVVE QTRSHQLGVL VLDYVNEEKD GTTRDEFRFK LNIAMGQFAE AAR DALEMA RFEQEEGNYR VAHDKLFGTV KQLEALNTKP PGELLRALML LHSYTLVKSL IAINDHTTAA RMLVRVARNI SKFP KHIVP ILTSTVIECH RANLKKTAFE YASMLMRPEY RDQVAVKYKK KIELMVRKPE KDPEELEEPL ADCPFCNMPG PETEL QCIS CQNILPFDLA TGKRMVLSDW AECPGCKFPA SASQFIRIIS AEGRCPMCND PVDLAHVRKV QDPLTKIKQQ QAQQTN ASG GGAS

UniProtKB: Intraflagellar transport protein 144

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Macromolecule #2: Intraflagellar transport particle protein 140

MacromoleculeName: Intraflagellar transport particle protein 140 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 157.426641 KDa
SequenceString: MEIYYTTKIS GRAGVIAGWS KAPGYPSLLA VALSPNAVGI YNEEGRPLDP NNEANIKNVR GTECAKIAWH PLLPLLAIGW KDGAISFWN AEERKLEEDS KIHRNTISSM TWTASGDRLI TGDENGRISM WKTDRLMRPI HVVAYDEPGA VIRHTVVGLP E EMPDTNSQ ...String:
MEIYYTTKIS GRAGVIAGWS KAPGYPSLLA VALSPNAVGI YNEEGRPLDP NNEANIKNVR GTECAKIAWH PLLPLLAIGW KDGAISFWN AEERKLEEDS KIHRNTISSM TWTASGDRLI TGDENGRISM WKTDRLMRPI HVVAYDEPGA VIRHTVVGLP E EMPDTNSQ VVVAFYVADA QERAVVKWCN DQGYSGVVVE MSEGVHTLVH YPERDQLLVV GSSCTLNVLT RDEQLGTWVT AS KMKFATG TGEAATGLQV AWAGNHTLAS ASEKDNVVRM YNFDTEDNYV LNLEQDSGLV SRVVCLAYDD RYNLLAVGTT DGR VMMYKF NQPNLNLEPV LDFAKCWEMQ PAFFVGNRAL AMEWGPFPRL MVVACNDAIN VCRKTMLSYK FRDGVAIMQV AVDR VVLEN LEVEPQRPPG RLQLQDMQLL GLDLSKGLLL VWDGERAEVY KVTETNDIMP ASQFETTSRC MAINNDSVYR AAENK VEVV NMAGTVKQTL MFDDNHGSPT SMDVARDYLA VVTSANIVRI LKVAGREAKP HAGPAPLCPP ELKDWKVMAM RANGNG NLV AALVVGPEPA RAVRLVVWCG ETNTHHVHDF TPEGRQPVQL MFDTVEPKLL VVQCTPVFTE ANVDERPHGD AAHVVDC AI IFVDPDKGIL LQEYQPIHTG GATACIGSCA PHLLTNKKSM VQPAPGSGAF QPFTSNVSKA IMTSFQGMQD SDDKTRRA L LDFSFNLATG NMDEAFRSVK AIKNPAVWEN MAHMCIRNKR LDVAEHCLSN MEHARGARAL REAKSIEEAD ARVATVAVH LGMIEDAKKL YIACERYDLL NQLYRACGQW DKALEVAEKN DRIHLKSTHY AYGQFMERQG DMEGARKHYE AAGCGLVEVP RMLFEAEKF TELQNYIQAN DSRELILWWG KYLESLGEYA KALDCYRKAG DSLSMVRIHC FQRDWKAAED EVTNSADNAA S FHLARQYE ASGRIPEAIR YYTLAKRYSH GVRLAKTHEL DSDLMNLALK STPAVMIDTA DYLFAKGQHE KAATLYMKGG KL SKAVEMC FQAQLFDVLQ HITDDMTPEK SDPNLYNKCA EFFMGFGHND KAVKMLIAAQ QYGRALELCV EHDVSITEEM ADS MTPDKN AAVSADERNN VICRIAKVAK RQGNFQLAAK KYTQAGDKVK AMKALLRGGD AEKIIFFAGV SRQKDIYLMA ANYL QTLNW HSDPELMKHI ISFYTKAAAW ESLASFYEAC AQIEVDEYRD YEKALQAMRE AAKYVAKSKN DDRDARVGVI NDRIA VAEQ FVAARQLIGS NPQEALRVCD ELLRAIPPNS QDLEAGIRIG DVYALMVEYW YEARNPNEAY KAIEAMRRRG IILSPY LDT RMVEDIYRSL GVALDMAEER RGPANLGLRE SDAGAFVEEE VADEDD

UniProtKB: Uncharacterized protein

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Macromolecule #3: Osm-6-like protein

MacromoleculeName: Osm-6-like protein / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 50.453184 KDa
SequenceString: MEEPGAEEVR ILFSTAKGES HTHKAGFKQL FRRLRSTYRP DKVDKDDFTL DTLRSAHILV LGGPKEKFTA PEVDMLKKFV KNGGSILIL MSEGGEEKAG TNINYFLEQF GMSVNNDAVV RTTHYKYLHP KEVLISDGIL NRAVITGAGK SLNSNDDDEF R VSRGPQAF ...String:
MEEPGAEEVR ILFSTAKGES HTHKAGFKQL FRRLRSTYRP DKVDKDDFTL DTLRSAHILV LGGPKEKFTA PEVDMLKKFV KNGGSILIL MSEGGEEKAG TNINYFLEQF GMSVNNDAVV RTTHYKYLHP KEVLISDGIL NRAVITGAGK SLNSNDDDEF R VSRGPQAF DGTGLEYVFP FGATLSVQKP AVPVLSSGKI AYPMNRPVGA VWAQPGYGRI AVLGSCAMFD DKWLDKEENS KI MDFFFKF LKPHSKIQLN DIDAEEPDVS DLKLLPDTAS LADKLKGCLQ EIDDVPRDWT SLFDDSLFKF DTGLIPEAVS LYE KLGVKK GQLNLIPPSF ETPLPPLQPA VFPPTIREPP PPALELFDLD ESFASETNRL ASLTNKCHGE EDLEYYIMEA GHIL GLKLQ ENANAKHVLS EVFRRIAQYK MGSLGLGQTL DSMGQTLPAA NQFGDQFEL

UniProtKB: Osm-6-like protein

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Macromolecule #4: Intraflagellar transport protein 80

MacromoleculeName: Intraflagellar transport protein 80 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 85.782609 KDa
SequenceString: MRLKVKQSSA NVHSELTAAV GWNVWNELFT CSDDQTIHKW NMLGEPEQKV STLDAYFTDM HWYPVSSKKT QAGGTDVFAV ACTDGSVKI LSRTGRVEKS IEGHKGACIS LRWSYDGTAL ATAGEDGSVK IWSRNGMLRS TLAQADSPVY SIVWAYDCDQ L CYCTGSNV ...String:
MRLKVKQSSA NVHSELTAAV GWNVWNELFT CSDDQTIHKW NMLGEPEQKV STLDAYFTDM HWYPVSSKKT QAGGTDVFAV ACTDGSVKI LSRTGRVEKS IEGHKGACIS LRWSYDGTAL ATAGEDGSVK IWSRNGMLRS TLAQADSPVY SIVWAYDCDQ L CYCTGSNV VIKSLSSNAK QNAWKAHDGV VLKVDWSPIN HLIITGGEDC KYKVWDSFGR LLFQSGLFDY PVTSVAWAPS GE LFAVGGF NTLQLCDRMG WAYSKIHLND TGSIMTLSWT ADSTQLAGGG GSGGVVFGQV VDLALEDGKM QVTVVDDMRI VVN DILNEN ADELPEFRDR VIKVSLGYGY LIVATATQCH VYNTTNLGTP HIFDLKDTVT LLLQAERHFL LLDNSAGIQI YTYE GRQIC NPRFQGLRTE LLNAQMITLS NDTIAVLDQQ ASGTTVRFFD TAQGRPVGEP WQHTLEVKEI ALSQAGTIND RQLIV IDRN RDLYLLPVMK RHVAKLAAMC DSARWHDSTA MLSAMVDQRL CVWYYPSEVY VDKDLLAKTR YTKSDSDFGK SAQIQL FAG NRCLVRRSDG VLVSAATSPY PAVLYDMIRK QQWDKATRLC RFIKDPTMWA TLAAMAMAAK ELNTAEVAFA AIDEVDK TH FVRKVKQIPT EEGRNAELAV YRRKPEEGES ILLQAGLVFR AIKLNIKLFN WERALDLATQ HKQHQDTVLW YRQQFLKN A KLAESITRFM QMNESVVVDQ AAVKKKIEEE RIKESQRPGA KRYV

UniProtKB: Intraflagellar transport protein 80

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Macromolecule #5: IFT54

MacromoleculeName: IFT54 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 54.437715 KDa
SequenceString: MCDNWQATID TLQGASPVFD KPKLSQKLLE KPPFRFLHDV VTAVQQATGF APGLYQGDEL DGKAIQEKDA KVAYLKKIIE VVSMVLGEQ CPARPNKIVA GLEPENTNIF LQMLGRACQK GNGAKAVQKV LGGGGAEPAP AKEEAPPPEK KPEKKEKKEE K PAEKSRAE ...String:
MCDNWQATID TLQGASPVFD KPKLSQKLLE KPPFRFLHDV VTAVQQATGF APGLYQGDEL DGKAIQEKDA KVAYLKKIIE VVSMVLGEQ CPARPNKIVA GLEPENTNIF LQMLGRACQK GNGAKAVQKV LGGGGAEPAP AKEEAPPPEK KPEKKEKKEE K PAEKSRAE ASPARKKAAE PEAEKKSSSK SSSRTKEEPP AKAPAKKKEE PAPEKPSKSK AAPAAEEAPP PPPPPAAEPP AR SASPGGE DPLNKSGSAA PKFQRPTSAR KAPPRVPQPQ QPTMLAGTGI RPGTATRRPN EPKPTDSKVT KPVAVFTDNA KDN SDDEVE VVHEQTPVLS GGANMTGEQG VLVKDILAAE KGLKKAGVDA TADNADTSDQ GSTGIILKRL GGKAAGAGAA AAGP RAHDP SSVRELVQKL CHSSTPLAKS MDYLQEDIEN MRKEYKFWLT EKRMYQDELA RELRLQGEAA NVDAQLADLD GQIKQ ARDR IIGMKGQILR NDETLGKLLA MATAGR

UniProtKB: TRAF3-interacting protein 1

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Macromolecule #6: Intraflagellar transport particle protein IFT20

MacromoleculeName: Intraflagellar transport particle protein IFT20 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.658664 KDa
SequenceString:
MDAVDRGVYF DEDFHVRILD VDKYNASKSL QDNTNVFINN IQNMQGLVDK YVSAIDQQVE RLEAEKLKAI GLRNRVAALS EERKRKQKE QERMLAEKQE ELERLQMEEQ SLIKVKGEQE LMIQKLSDSS SGAAYV

UniProtKB: Intraflagellar transport particle protein IFT20

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Macromolecule #7: Clusterin-associated protein 1

MacromoleculeName: Clusterin-associated protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.733469 KDa
SequenceString: MSFRELRSFT EVMKALGYPR LISMENFRVP NFELVADCLY WLVHRYYPGV EIADDISTES DRVKFLQSVA QVMLTKARMK LNIKRLYAA DGNAVKELLK LASLLYKATS KAGDMDDDAA EAIDVANSLK GFNPKEIKGL AAEIIKAGAG LYDALGQEPE L REHRARAV ...String:
MSFRELRSFT EVMKALGYPR LISMENFRVP NFELVADCLY WLVHRYYPGV EIADDISTES DRVKFLQSVA QVMLTKARMK LNIKRLYAA DGNAVKELLK LASLLYKATS KAGDMDDDAA EAIDVANSLK GFNPKEIKGL AAEIIKAGAG LYDALGQEPE L REHRARAV AGHVDTDFVE RSIREAIAQV EDNIRSLENQ MEDLERNEKT LDNKIEKKKQ ELERQEKRLS TLQSVRPAYM DE YERLQGE LNGLYQSYLD RFRNLEFLEN ELEAYYQAEQ EKMEAQERRL KKMQKRLKEE ELRILRGEQE VDEANMNFDD DDM DLSEDG EHEEAEEGED EDYGAGGQKA TMRRDQFGGQ GGGGGGGRQQ GGGRNVMGNL DGDFDDEASI DEDDNTDGGE VSRQ GSQAG GMGGGGGYGG GGGYMNGGGM DDDENNLIQD DDEDDNDF

UniProtKB: Uncharacterized protein

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Macromolecule #8: Intraflagellar transport protein 74

MacromoleculeName: Intraflagellar transport protein 74 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 71.648398 KDa
SequenceString: MDRPSSRGAL ALGAGGLGKA PTGGAVQQPD RPMTGQRGAA PAGPMRAPAG ASIIGAGPPG TAMRGGPGPA GGPPGTAYKR MGTASQRPG TGQQAAAAAA AARAGQQLQV ENRPITNHGV SGMKTAAAGV GRQVLDKNYF MNELRQKRME IAQVTSNMKS D LEALERKQ ...String:
MDRPSSRGAL ALGAGGLGKA PTGGAVQQPD RPMTGQRGAA PAGPMRAPAG ASIIGAGPPG TAMRGGPGPA GGPPGTAYKR MGTASQRPG TGQQAAAAAA AARAGQQLQV ENRPITNHGV SGMKTAAAGV GRQVLDKNYF MNELRQKRME IAQVTSNMKS D LEALERKQ AQYNSMDKRA SDLSKEVKIL QEALADYNTV LDKVGSQAPV YVIQQEFAAL KDRNEQQRKR VDEVLTERLN LE SKAKQAE SKMSEIQASM DQRLNSMPPS QRNEYTTLVA EQQQLQADSK RFEEVLDELD KALQASEGEL ARNPFKQRSL QLQ EQIRAL TEKKYELTEE ERQSKRSPEE LRADLMAKIK RDNTEVEQMT QQIRELQDQI KKMEERVKSL GGATSGAAAA EEKA NREKF EELLAKERDL NNFMDGFPSR KAAKMQEKQQ KEDGIVGVLE KMVKMQGIIG SNLPSQKKYK EMQDELEYKK MQLEN TQTT QERLKEELTM RRTELEKIDT LEDKIKLELT QLAERQEAME KEMGEFGSVE DIQRKANAAR ERMEGLRSVL LKRKDL LRS IVAERGLKFQ AKRAQLQDHN LQVQLEKMEA KLKNLSAGVF EMDEFIKAKE SETNYRQLAS NIAALVDDLN VHVKKAV V

UniProtKB: Intraflagellar transport protein 74

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Macromolecule #9: Intraflagellar transport protein 81

MacromoleculeName: Intraflagellar transport protein 81 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 77.186484 KDa
SequenceString: MGDVSYIVDS LGLPPFSYQM SLLSFTEKGP QELLQLLSDV FSTISPKHQK VDVAKEVPDQ TADRLIGFLK IIKYRPNVQD PLLFRQLVA AGDRETLYQI LRWVVPQAQL LEKRAFVGYY LSFPDMPEEF NFDPDIMELK EEIKAMQQEF IELHKSSDAI K TLSKDTQA ...String:
MGDVSYIVDS LGLPPFSYQM SLLSFTEKGP QELLQLLSDV FSTISPKHQK VDVAKEVPDQ TADRLIGFLK IIKYRPNVQD PLLFRQLVA AGDRETLYQI LRWVVPQAQL LEKRAFVGYY LSFPDMPEEF NFDPDIMELK EEIKAMQQEF IELHKSSDAI K TLSKDTQA LKNKIKSLEE EKERLGEKVE RAKGAVDKLP DRSSYMEVCT NLRKQQDEEV NLSTAIQTQR ALQEKAEASY HR AAARLRE LQTSYQEGSA GKLLETLNED VKNLRAQVNE RYPKEVEKRQ KRQAALSEAL ASGISTEMDL QRLQHQATAL HNQ ITEIQE RKVAQDKARQ GDKAYLQLRQ AQQMATVSAR KKEELGAKQE RLQEKKTALT AQLDKLNAEG GGSGAVFSEE EWRT KYESM KSKLPIYKKM KKELGDLEAE VFVLAHTEEL LASQEGGLLE KVKRLEKQQG ISGFTETAQH LEKVSEAKSQ MDEEK GMTL IEISRTVEEI NNAINQRKQQ LAPQIKKLRS VRQDFAEFEA KYLEKKTAYD NVVATFEART SALEGEVSGL KAEVSE NET KYHMLHCQLH ITDQNIKKVT SGPAAERLRD KYEAKVKEAE DSTKALRDRQ REIKDTHSTG LSQIDIMNDM LRLLQLK LN LARGIAVDMS QYGGGGGAAG GANGGMAGQT YDTGSANVLQ L

UniProtKB: Intraflagellar transport protein 81

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Macromolecule #10: Intraflagellar transport protein 27

MacromoleculeName: Intraflagellar transport protein 27 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.783967 KDa
SequenceString: MVKKEVKPID ITATLRCKVA VVGEATVGKS ALISMFTSKG SKFLKDYAMT SGVEVVVAPV TIPDTTVSVE LFLLDTAGSD LYKEQISQY WNGVYYAILV FDVSSMESFE SCKAWFELLK SARPDRERPL RAVLVANKTD LPPQRHQVRL DMAQDWATTN T LDFFDVSA ...String:
MVKKEVKPID ITATLRCKVA VVGEATVGKS ALISMFTSKG SKFLKDYAMT SGVEVVVAPV TIPDTTVSVE LFLLDTAGSD LYKEQISQY WNGVYYAILV FDVSSMESFE SCKAWFELLK SARPDRERPL RAVLVANKTD LPPQRHQVRL DMAQDWATTN T LDFFDVSA NPPGKDADAP FLSIATTFYR NYEDKVAAFQ DACRNY

UniProtKB: Intraflagellar transport protein 27

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Macromolecule #11: Intraflagellar transport protein 25

MacromoleculeName: Intraflagellar transport protein 25 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 20.380588 KDa
SequenceString:
MKDYAREENG GLVVMASCSD ERFPPENMLD GKDNTFWVTT GMFPQEFVLR LESCIRVSKI TTLSLNVRKL AVEKCDQDKP DQFEKVFEV ELANRGDRLQ TEVHQVNIRA KYLKFILLQG HGEFATVNRV SVVGGDDDGG GYDEPGGGYG SMQRQPSMGY G GGGGSAAT GFAADPGNAA AGGGGGFEDE F

UniProtKB: Intraflagellar transport protein 25

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Macromolecule #12: Intraflagellar transport protein 46

MacromoleculeName: Intraflagellar transport protein 46 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 37.97641 KDa
SequenceString: MDDSMDYPDR DGDDLDQFQG TARSQVVQNQ PHDEEVNLSE SESFAGADEP PAAPRDASLI ESHDMDEGPA APARTLSPTG YEAGKHAPG GIANSDEAPP GAYNAQEYKH LNVGEDVREL FSYIGRYKPQ TVELDTRIKP FIPDYIPAVG GIDEFIKVPR P DTKPDYLG ...String:
MDDSMDYPDR DGDDLDQFQG TARSQVVQNQ PHDEEVNLSE SESFAGADEP PAAPRDASLI ESHDMDEGPA APARTLSPTG YEAGKHAPG GIANSDEAPP GAYNAQEYKH LNVGEDVREL FSYIGRYKPQ TVELDTRIKP FIPDYIPAVG GIDEFIKVPR P DTKPDYLG LKVLDEPAAK QSDPTVLTLQ LRQLSKEAPG AKADMVGRLE HTDENKAKKI QQWIASINDI HKAKPAATVN YS KRMPEIE ALMQEWPPEV ETFLKTMHMP SGDVELDIKT YARLVCTLLD IPVYDDPVES LHVLFTLYLE FKNNPIFRQH MEM ENKLDG MSGGGGGMMG GGADVLGL

UniProtKB: Intraflagellar transport protein 46

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Macromolecule #13: Intraflagellar transport protein 22

MacromoleculeName: Intraflagellar transport protein 22 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.363285 KDa
SequenceString: MAEHGLKIAV VGPQRTGKTL LCRALAEQPI LLGEMSYQPT AAVRIQEISR VLGIDRVKVQ FWDVSGSVQY QSYWPVLAKE VDGLLMVID PNRPEQERDL ETFYRNFAEP NNLYTRQCMV MAIQVQKEGG GLGGWQGLQG GLKKLSQSYV AINPANPAAG V QEAYSHLD ...String:
MAEHGLKIAV VGPQRTGKTL LCRALAEQPI LLGEMSYQPT AAVRIQEISR VLGIDRVKVQ FWDVSGSVQY QSYWPVLAKE VDGLLMVID PNRPEQERDL ETFYRNFAEP NNLYTRQCMV MAIQVQKEGG GLGGWQGLQG GLKKLSQSYV AINPANPAAG V QEAYSHLD VLFQGALQSK KEALESSYMN QEDQ

UniProtKB: Intraflagellar transport protein 22

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Macromolecule #14: Intraflagellar transport protein 172

MacromoleculeName: Intraflagellar transport protein 172 / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 197.851172 KDa
SequenceString: MQLRYFKSIL PPADQYQKIT SLTWAPNNSR LAAVSTDKVV YLFDENGEKR DKFKTKAAEA NNPNTYIIRA MAFSPDSTKL AIAQSDNIV FIYRLVDPDT GAEKKSICNK FPQACAVTSL VWPKDRPNEV VFGLADGKVR LGMLKNNKSY TCYAHPENSY V VALASSLN ...String:
MQLRYFKSIL PPADQYQKIT SLTWAPNNSR LAAVSTDKVV YLFDENGEKR DKFKTKAAEA NNPNTYIIRA MAFSPDSTKL AIAQSDNIV FIYRLVDPDT GAEKKSICNK FPQACAVTSL VWPKDRPNEV VFGLADGKVR LGMLKNNKSY TCYAHPENSY V VALASSLN GQNVISGHMD GAIWKFNFPA EEGGTPTSSQ LVVHSCVPYS LGWGSCIAAA GNDNRVVFYD LNGREIRSFD YS NNDEVRE FTTCAFNPSG DTVVFGTYNR FYMYTFNIQR NDWEEAGHKQ IDNFYAVSAA SWKPDGSKMT VGSMTGAVDM YDA CVKRHM YKGKFEFTYV SKSAVIVKTL KTGMRIVLKS VYGYEIEKIN IYHDRYLIAR TTYTLLMGDL DTCKLSEIPW DSDG SEKFH FENERVCMVH YAGELHIVEY GRNDVLGTCR TEHMNPYLIS AVVQEARGIA SESKKLAYLI DLQTVRIQDL MAPVG STLA TVNHDTKVDW LELNQRGTHL LFRDKKRHLH LFSLSGQERT TLLNYCQYVQ WVPGSDVIVA QSRNNLCVWY SVNKPD NVT MFPIKGEVVD IERHNHRTEV IVDEGINTVS YALDEALIYF GAALEDQDYE RAVQTLEPLE LTPETEAQWM QLAEQAL AT NQLVIAERCY AALGDIAKSR FLHKVVKKAQ QAAKEFGGDG TDAWSVRAMM AQLNKQWPVS ESLLLAQGKV DDAITLYQ D NHRWEDAIRV ADSTHHANAA ALKQQYLTWL LETGQEEQAG AVKEREGDYL AAIGLYLKGG LPGRAAQVVM SVHNVNWDP ALLDSILASL AKAGLYERAG ELYEHMSRSS EAMQSYRRGH AYRKAIDLAR REFPAEVIII EEEWGDWLVT QKQMDAAINH FIESGATLK AIKAAIDCRQ FAKAAGIIEV LDPREAMPYF RRIAQHYETT GALEEAERYY IRADMARDAV EMYSRAGKWE A AQRVARGY LTESEMRAFY RAKAAEFEAA HKLKEAEKAY LAAGGDDVDK AIAMYKRNKM YDQMIRLVTQ YRKEKVPEAH TL IAQQLEV EGNLREAEKH FVEAKDWKSA VQMYRQVNQW EDALRVAKVY GGVNASKQVA YAWALTLGGD DGAQLLKKMG LLD HAIEYA VESGAFAQAF EMTRAGAKHK LPEVHLKYAM FLEDEGRFAE AEAEFISAGK PKEACDMYMH NQDWDAAMRI AERY DPTMV SEILVSQARV AVERKQWLPA EGLFIKAKRP EAALKMYRDA RMWNDALRVA EQYLPTKVAE VQMELLSGQG AGGGS GGAS ADAVINKARG FERNNDYARA IETYLSLTAQ DTSNQDQLEH CWGQAAQLAI NYQRHRMKDV VNTVSERLQE IGRHQA AGE LHESIDDAQG AIRAYCAGRL WDKARTLAGT NPTFSRYIED QYNNYLLQNQ QADELASRGG QHAQQAIEMY VARDEWA KV HELAAQQGPE VASNYALKHA ERRFKQGDYA QAAQVFAQHG ITAQPQYFEL YKSIAQGVLH ASQGDRNPVA EKSLRDMM Y RLVNVLRSGG GAGKYKVDTD AFQNYYLAAH YLTCAAAAKE QGLKDIAAMN LTSVLRYVGP TIPADRAFYE AGLAWYEAG RKNMAFVMLN RFLDLSDAMD EPDSSAAVIE NADFSDTDIP YDFTIPERAY CTESQREDVR NLVLEISMDR SSDQSLALKA CEHCGKPTY EANLTCHFCK KKYDPCVVTG YPIQSYDRVV FKNNGPELNA IRDMWNKWVE AFGTDPVTGM QAAPMY

UniProtKB: Intraflagellar transport protein 172

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Macromolecule #15: Intraflagellar transport protein 122 homolog

MacromoleculeName: Intraflagellar transport protein 122 homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 139.382922 KDa
SequenceString: MRTVVAWQET PPEKDGVRNV CYDLCFKPDG SQVVAGIGNR VLVYDAADGD LLHALKGHKD SIYCVAYAQN GKRFASGGAD KTVIIWTSK AEGILKYTHN DAIQCLSYNP VTQQLASATG SDVGLWSPEQ KSVAKHKVAS RICSISWTAD GAYLALGCFD G VISIRDKG ...String:
MRTVVAWQET PPEKDGVRNV CYDLCFKPDG SQVVAGIGNR VLVYDAADGD LLHALKGHKD SIYCVAYAQN GKRFASGGAD KTVIIWTSK AEGILKYTHN DAIQCLSYNP VTQQLASATG SDVGLWSPEQ KSVAKHKVAS RICSISWTAD GAYLALGCFD G VISIRDKG GSEKHRIETG PSPVWSICWN PVETNVLCAG CFDGVLKFYM MSGQQKSKDR ELAFDPLCVS YFSSGEYITV SG TDKTVHL YTRDGTYLTK IADRDSWVWA VRPRPKHNFV AVGTEGGGIA MFQLIFSTVH GLYQDRYAYR DQMTDVIIQH LIT EQKVRI KCKDYVKKIA VYKDKLAVQL QNKVVIYELA NADDFDMHYQ SATKIQQKLD CNLLVVTSHH VILCQEKKLQ LYNF DGVKE REWVLDSVIR YIKVVGGPPR REGLLVGLKS GAILKIFVDN PFPIPLIKHT ASVRCLDLSA SRNKLAVVDE NAKVL VYNL ITKELVFEES NANSVAWNSE FEDMFCYSGN GMLSIKTGDF PLHQQKLQGF VVGFKGSKIF CLHYVSMQTI DVPQSA SMY RYLERKDFES AYRVACLGVT EADWKQLALE ALQALNLEVA RKAFIRIRDV RFVELVNRTE AGRKAGTSEQ LLLAEIM AF QGRYQEAARL FTQAGAVDRA MEMFSDLRQF DEAKKWAEEF AASGRGDQRS VQELINRQAE WSEEVKNYDA AAEMYIKA K KYDRAIAILA KHQWWDKLIG VVRQLDKTDA RCLGMCAGHF RRAPHFAYAK ETLLKMDDTK GLITLYVEAE KWDDAFLLL HAHPECRQDV YLPYAKWLSN QDRFDEARLA YQEGGFPSLA TRILEQLCAN AVVETRYADA AFYYYQLAME ALKSIKNPPS NMAPSDRSA LERFTELYDR AEVYYAYEVV HKSVHSPFRT THPDTLFNAS RFLLMRLLPP REVPLGVSVV NVVYVLAKQA V EAGAFKLA RFAYNKLQTL VLPAAWQAEV DLASVVIRSK PFSDKEDLLP VCWRCSTTNP LLNTQGDYCI NCGAPFIRSF VT FEHLPVV EFELEPGVDD EEAGRLLGED AGMEAARRER KAERQAKAAE VGGNMLRLDQ NEIDRMDDAF AAQMMVPNTT IRV DRAMLR RLKTAEVMVR TWPNPVIPKQ YFRVMDQEVP LCCGPCGHFF EQDEFEMAAL ERGTAPFSRT TVRGEGLAPG EDAE DEGAG GNKLGGPLGS ARGPIGGASK ARMSVPFQQG RPLV

UniProtKB: Intraflagellar transport protein 122 homolog

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Macromolecule #16: Intraflagellar transport protein 121

MacromoleculeName: Intraflagellar transport protein 121 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 136.267359 KDa
SequenceString: MFIYLSKKIA IPNNVKLRCI SWNTDQGWIA CGGESGLLKV LRLDGTTSKD VKGKKEGGAP GGNLSLNQTL EGHNGAVICV CWNNAFQKL TTSDEFGLII VWMLHKGMWF EEMINNRNKS VVKDMKWTSN GEKICIVYED GAVIVGSVDG NRLWGKELGM D LALVEWSP ...String:
MFIYLSKKIA IPNNVKLRCI SWNTDQGWIA CGGESGLLKV LRLDGTTSKD VKGKKEGGAP GGNLSLNQTL EGHNGAVICV CWNNAFQKL TTSDEFGLII VWMLHKGMWF EEMINNRNKS VVKDMKWTSN GEKICIVYED GAVIVGSVDG NRLWGKELGM D LALVEWSP DGRLLLFATP GGECHVYDGN GNGVAKVPLY CNEGYAGASK IIGVEWYDGV EGYAEPNCPV LAICLDNGRM QL MRYESDD NAVCIDTGIK PVKVKWNMNG TILAVAGFQM SSAVADGREL WMVQFYNHNG EHLRTLRVPG GGISGISWEG NGL RLALSV DSYVYFANVR PDYKWGYFCN TLVYAFNRPD RSEHCVMFWD TKNNDKYPKY VRKLVAIQAF GDFCVLATKG ENPG EHILI LCNAIGSPVD SKYIEVEPKY LAITNYHVIA ANDEVVYVWQ FRTSFSKVLS TDINAVKRKD VREKMFSVDD SNPAL SDRS PESFRNMTTQ TTDPIACVTA CDSVLMIGRA SGVVNRYSLP HLTLDAQHVL RCRPQMLALN CNLSKMSIID INGVLT FFD LTAKAPGGGA NTMGEHLSFE RKDAWDMRWA DDNPELFAMM EKTRMYIFRG LDPEEPVTSS AYLCSFHDLE ITAAFLD DI MQQPDQPDLE FMVMYETRSL RDTRELLKSA SVDDAYAFVD SNSHPRLWRN LAEHALEQLD FTTADKAFVR CADYQGIQ F VKHLGKLDDK AKQRAEVAVY FKRFDEAEQL YMRMDRPDLA IDMRMRLGDW FKVERLIRES SGDDAQLLNA HNKIGQYYS DRHKWGKAAQ YYAQAKNSEM LVECFYALED FVALGRLMDA LPDGSPLLAN IGEKFQSVGL CNEGVTAFLK AGDTKRAIDC CVLLNQWDQ AVQLAQAHNF PQIEQLLAKY ANHLLEKEKV MDAIELYRKA NHSMEAARLL QDLAKKCADQ KVHPLRVKKL Y VLAALEIE KFKKRTLDMA GPDKTAATLL GTGGTATMAA TAAQTLAGLM TLESAVSGEA GVDSAWRGAE AYHFWLLAHR QL YAGNVDL AMRTALHLRE YEDLLDPVEI YSFLALAAFY NQFFGQCSKA FIKLESMPSI PNDKRESFAD LAMSIFLKHP PAD PRALRE TREKKAPGGG AMAGNSLDAL LEDLGGGREQ VCVASGRIVR DGNVVRCKVC KHLSITHELH GAAVCPLCHA ALPV SGGGA PGRGMSAKGG AMAQMYGAY

UniProtKB: Intraflagellar transport protein 121

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Macromolecule #17: Intraflagellar transport protein 139

MacromoleculeName: Intraflagellar transport protein 139 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 152.227688 KDa
SequenceString: MADRVLALVH YYAREGYFRH VQTVCNEVLK KRPGDGVLTF WRAYGLLMEG NTADAMRDLS SIQGNSDLEL AVAAAQLLGH ESAKVPDHD AIIDLQAKLE IEERTASDQP CLHLASFYLY TKSKERARGL VERVLRNQPD MVPAQVLLGW IIISQQQDDE Y DMLFDESE ...String:
MADRVLALVH YYAREGYFRH VQTVCNEVLK KRPGDGVLTF WRAYGLLMEG NTADAMRDLS SIQGNSDLEL AVAAAQLLGH ESAKVPDHD AIIDLQAKLE IEERTASDQP CLHLASFYLY TKSKERARGL VERVLRNQPD MVPAQVLLGW IIISQQQDDE Y DMLFDESE LDDALSHFEQ AVEHDHNDLQ ALLGKAKIME LKKQLGPCLD VLTEINVRFG WFVPALVEKT RMLMMLGDWE QV TETLQRV LAADQQNIMA QAWNCMISLT REGNNKQAAK QLQDLFSSMN RQEPKNAELF FRVARPFGRL ACSDPTLLGI TYL MADRAA QLRPEMAAYV VEAAAQKLMM DETTNATERF TQALQLDELN LEANAGALEA QIMAGELEEA AGQIMFLEDM FTNA AAAGG GKRKGRGTGD MDDDPDMADP SLGTSSDNPT LLYLKGLLAW KQGMPSEGLG LLERSIAALF SAAADFHGPS LELYA ALNP ARITAVVRLL LQSIGGEPRA PTEAPSPLIS KVTRALDLLN KQAPALQESA LLHARALYLN GNLDGALRKA GEILRM NPE ESSAHLLICS VYVAQDKPEL AVSALDQAVS SNFAIRETPL YHVVQAKVLV ANNKLDDAKR VLESAMNLPG VRTALTV QQ RARLGRKVVE PTLHERATVY LLLADVLARQ SKIPDAPEAK KYIQDAIREF EGTSEEVRVT VADCELAIAR GDVEGALK K LRRIPKESPH YVKARMAMAD IYLRHRKDKA AYIKCYMDLV DHTPDYDSYC MLGEAFMQIQ EPEKAVRAFE SALEFSPKD VDLITRCARA LVTSHDYQRA IDYYTKAIAN ARGGAQHALQ LELGNLLVRL RQYPAATAAI NKALERNRDG LPATENLQLD VEAWSMLAK VHKGKLEMEG YSQAQSRALE LQKQLLLKLR GELPEAVALQ RERTAAICFD LAEQSKRARQ FDRAMELYME A LRHHDTHV PSMLAVAKLH LANGDTDACQ AQCVTLLKHD PDNEEASIML AELMFHKEHY DTAIYHFQQL LERSPNHYGA LA QLILLLR RAGRLEDVPR YFALAEAGSP KAVMDPGYHY CKGIYNRYIN NPREALKELN LARKDTRWGS QAILHMVEIY LNP DNDAVW EEKENADTPE SRQAVATARS LLKQVRGADT TTQRYRVLES YAIMAGKDKN EIEASLNVLL DLANQDPNNV PVLL AMATG FMMLKQTPKA RNQLKRVQKI QYKPDEAEEF ERSWLLLADI HIQGGKYDLA QDLCQKCLKY NKSCAKAWEI MGQIM EREQ AYKDAADHYE NAWKHENQAS AQVGFKLAFN YLKARRYVEA VDVCHKVIKA FPDYPKIRKE ILEKARMGLK P

UniProtKB: Intraflagellar transport protein 139

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Macromolecule #18: IFT88

MacromoleculeName: IFT88 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 86.418125 KDa
SequenceString: MSYGGTEEDD LYGGYDEQSN PLAGSGGAAF KALGADGAPP GTAMMGPPGT AMKSFVPGTA MRGGTAMQQD PSLARPMTSN RGAGFTSAP NKKFDPLNRS MGSTLGSSGG GAMLVARKGD TSPEEQARGM EKTVHELLEK SAADAAKNDI NSALENAMEA K KNERKLCR ...String:
MSYGGTEEDD LYGGYDEQSN PLAGSGGAAF KALGADGAPP GTAMMGPPGT AMKSFVPGTA MRGGTAMQQD PSLARPMTSN RGAGFTSAP NKKFDPLNRS MGSTLGSSGG GAMLVARKGD TSPEEQARGM EKTVHELLEK SAADAAKNDI NSALENAMEA K KNERKLCR FREQNNMADQ INLELMYAVD FNLAHMYHMN KNYSEALNLY TAIVRNKNFP QSGWLRVNMG NIHFEQKKYP SA IKMYRMA LDQISATAKE VRFKIMRNIG LSFVRMGQYP DALQSFATVM DNVPDHQTGY NLVMCNYALS DREGMKNAFI KLL KVSPPS EMDDDDDDDP MGDDDMQVMT MDDGLKDEMR KRNTIITRLI VKAAQLISEK VDRANGFEGG FMWCCEQLRD AGYT KLANE VELAKATRFM GQKQFDKAVG VFKDFEKKEP RVKARAATNL AFLYFLEGET DQADKYSEMA LKSDRYNARA YVNKG CVLV ERGDLEGARS LFNEAAGIDP YCVEAIYNLG LVSQRLNELP YALAAFKKLH NMVPDNVEVI HQIATTYDMM GDFKNA VKW FELLTSLVSN DPGVLARLGA IHARFDDEAK ALHYYQESHR VYPVNMDVIS WLGAYHVKSE VYEKAMPFFD LASKIQP QE VKWALMVASC YRRTNNLPAA LGKYKQIHTQ HPDNVECLRY LVHLCSELGR RAEAAEYMTK LKKAEKAAVP EATTAAAP A AAAAGSGMGG MGGLDDDIGS SAVSAQNRGK KMLVKEHMGG GGGKDNDDWG NEQLGDDLLP M

UniProtKB: Uncharacterized protein

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Macromolecule #19: IFT70

MacromoleculeName: IFT70 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 74.248609 KDa
SequenceString: MAFFQQPARP IAEGQYTQTI YTLIKEQKFA EAIQHLQYQL QNVPESRAAL SLLGYCYYYT GQYDMASQMY EQLVTLYPSN EDYKLYYAQ SLYKGGMYPE ASKAVVKVEG HQKAVTTLLV ACSYEQDDLT GCRRQLDKCA PEDPDTMVNT GCIMFKEGKF E AARQKFND ...String:
MAFFQQPARP IAEGQYTQTI YTLIKEQKFA EAIQHLQYQL QNVPESRAAL SLLGYCYYYT GQYDMASQMY EQLVTLYPSN EDYKLYYAQ SLYKGGMYPE ASKAVVKVEG HQKAVTTLLV ACSYEQDDLT GCRRQLDKCA PEDPDTMVNT GCIMFKEGKF E AARQKFND AVQALGYQPE LLYNIALCYY KTKQFGPALK HLAEIIEKAV REHPELSVGS NTDGMEVRSV GNSQTLKETA LI EAFNLKA AIEYTMKNVE AAKEALTDMP PRAEEELDPV TLHNSALINM DSDPTGGFKK LNFLLQSPPF PPETFANLLL LYC KPSHGF YDLAADVLAE NPQYAGKLLS PDLYDYLQAA IGRYKSPEEA FRRFDELATR HVEQLRRLTK QIQDARIARD NDAI KRAIN EYDEALEAYI PGLMAMASIY WDMELYSNVE KIFRQSAEFC SEHEVWKLNV AHTFFMQDNH YKEAIRYYEP VVKKN ADNL LGVTAIVLAN LCVSYIMTSQ NEEAEELMRK VEKEEERSSM QDPDKPCFHL CIINLVIGTL YCAKGNYEFG VSRIIK SLE PYDKKLETDT WYYAKRCFLA LIENLAKHMI VLKDSSFTEI MAFLNEAEKH GKDIRVVFNE GKHQSRTIAS EARMLKK MF LKLRD

UniProtKB: Uncharacterized protein

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Macromolecule #20: Intraflagellar transport protein 57

MacromoleculeName: Intraflagellar transport protein 57 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 51.374508 KDa
SequenceString: MSSKRGGRSS LAKAPEEAVN GEAFAPEASP PPPGDDGDAG GEDGGAPAPP PPPATKGGPV AVGRSLEIQT TPDVCMEMLA DKLKLLNYE ADFCRKKKPY RKPLSRLYFA VPLANSSEQF FYFTSLATWL LGLAGVELPA PKEFDDPNLT CQNILGAVKK L GFAPPSYH ...String:
MSSKRGGRSS LAKAPEEAVN GEAFAPEASP PPPGDDGDAG GEDGGAPAPP PPPATKGGPV AVGRSLEIQT TPDVCMEMLA DKLKLLNYE ADFCRKKKPY RKPLSRLYFA VPLANSSEQF FYFTSLATWL LGLAGVELPA PKEFDDPNLT CQNILGAVKK L GFAPPSYH PTKLTVGNGK EVVGVLDGLV DFVLERRHHK YSRPAYGNDG QPEEGVQLDD EAEAAAMEGA DELAMPAQNQ AD DDEEEEG VYVDPGRGDA AGPGTGASAA MDAEKAVLVS KVDPTLWKIE LERVAPKLRI TIAADSKDWR SHLDEAHQHK EVI SKAWPD SKTSLERLRA DLNGTLEKLQ TREKFLNEQF ESLMQQYRAA RTTFTDVQET YNRKTEAVAD RNQEMHRIGE TLEE VKAMM DEKGSNIADA TPVARIKTAI KQLNKELHDM EVRIGVVSHT LLQLSLRNKR LLQAQAALSD EEED

UniProtKB: Intraflagellar transport protein 57

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 2.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 15.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1.3)
Details: Composite map, with constituent maps ranging from 15.4 to 28.3 angstrom
Number subtomograms used: 5896
ExtractionNumber tomograms: 756 / Number images used: 10455
CTF correctionSoftware - Name: CTFFIND (ver. 4) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Final angle assignmentType: MAXIMUM LIKELIHOOD

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About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

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