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- EMDB-19448: Alpha-synuclein amyloid fibrils with DNAJB1-Helix V mutant, HSC70... -
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Open data
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Basic information
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Title | Alpha-synuclein amyloid fibrils with DNAJB1-Helix V mutant, HSC70, HSP110 and ATP | ||||||||||||
![]() | Tomogram of alpha-synuclein amyloid fibrils incubated with a variant of DNAJB1 mutated in helix V, Hsc70, Hsp110 and ATP | ||||||||||||
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![]() | Chaperones / ![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | Saibil HR / Monistrol J | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Stepwise recruitment of Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibre disassembly Authors: Monistrol J / Beton JG / Johnston EC / Saibil HR | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 6 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.8 KB 11.8 KB | Display Display | ![]() |
Images | ![]() | 181.2 KB | ||
Filedesc metadata | ![]() | 4.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Tomogram of alpha-synuclein amyloid fibrils incubated with a variant of DNAJB1 mutated in helix V, Hsc70, Hsp110 and ATP | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.8 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Alpha-synuclein amyloid fibrils incubated with a helix V mutant o...
Entire | Name: Alpha-synuclein amyloid fibrils incubated with a helix V mutant of DNAJB1, HSC70, HSP110 and ATP |
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Components |
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-Supramolecule #1: Alpha-synuclein amyloid fibrils incubated with a helix V mutant o...
Supramolecule | Name: Alpha-synuclein amyloid fibrils incubated with a helix V mutant of DNAJB1, HSC70, HSP110 and ATP type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | ![]() |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.5 Component:
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Grid | Model: C-flat-2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber temperature: 277.15 K / Instrument: LEICA EM GP | ||||||||||||||||||||||||
Sectioning | Other: NO SECTIONING | ||||||||||||||||||||||||
Fiducial marker | Manufacturer: EMS / Diameter: 10 nm |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Specialist optics | Energy filter - Name: TFS Selectris |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 116.0 e/Å2 Details: Note: the original pixel size for the tomogram recording was 1.94 A/pixel. This was subsequently rounded to 1.9 A/pixel and the 2x binned tomogram deposited here is listed as 3.8 A/pixel. |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Software - Name: RELION (ver. 4) / Number images used: 121 |
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Details | Note: the original tomogram was recorded at 1.94 A/pixel, but this was rounded to 1.9 A/pixel, so the 2x binned tomogram provided here is at 3.8 A/pixel |