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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Closed Complex I from murine liver | |||||||||
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![]() | respiratory chain complex / mammalian mitochondria / MEMBRANE PROTEIN / ELECTRON TRANSPORT | |||||||||
機能・相同性 | ![]() Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / protein insertion into mitochondrial inner membrane ...Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / protein insertion into mitochondrial inner membrane / mesenchymal stem cell differentiation / circulatory system development / blastocyst hatching / respiratory system process / psychomotor behavior / Mitochondrial protein degradation / cellular response to oxygen levels / stem cell division / respiratory chain complex / response to light intensity / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / neural precursor cell proliferation / positive regulation of mitochondrial membrane potential / [2Fe-2S] cluster assembly / adult walking behavior / cellular response to glucocorticoid stimulus / oxygen sensor activity / response to hydroperoxide / iron-sulfur cluster assembly / positive regulation of ATP biosynthetic process / dopamine metabolic process / adult behavior / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / positive regulation of execution phase of apoptosis / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / neuron development / quinone binding / cellular response to interferon-beta / ATP synthesis coupled electron transport / cellular response to retinoic acid / negative regulation of reactive oxygen species biosynthetic process / extrinsic apoptotic signaling pathway / neurogenesis / tricarboxylic acid cycle / muscle contraction / Neutrophil degranulation / visual perception / cerebellum development / reactive oxygen species metabolic process / DNA damage response, signal transduction by p53 class mediator / aerobic respiration / regulation of mitochondrial membrane potential / respiratory electron transport chain / kidney development / fatty acid metabolic process / mitochondrion organization / response to nicotine / sensory perception of sound / response to cocaine / monooxygenase activity / electron transport chain / mitochondrial membrane / brain development / : / multicellular organism growth / response to hydrogen peroxide / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / cognition / circadian rhythm / positive regulation of protein catabolic process / NAD binding / fatty acid biosynthetic process / cellular senescence / FMN binding / nervous system development / myelin sheath / 4 iron, 4 sulfur cluster binding / neuron apoptotic process / gene expression / response to oxidative stress / in utero embryonic development / response to ethanol / electron transfer activity / response to hypoxia / mitochondrial inner membrane / nuclear speck / nuclear body / mitochondrial matrix / inflammatory response 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.9 Å | |||||||||
![]() | Vercellino I / Sazanov LA | |||||||||
資金援助 | European Union, 1件
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![]() | ![]() タイトル: SCAF1 drives the compositional diversity of mammalian respirasomes. 著者: Irene Vercellino / Leonid A Sazanov / ![]() ![]() 要旨: Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies ...Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies have shown that different tissues/organs are equipped with specific sets of supercomplexes, depending on their metabolic needs, the notion that supercomplexes have a role in the regulation of metabolism has been challenged. However, irrespective of the mechanistic conclusions, the composition of various high molecular weight supercomplexes remains uncertain. Here, using cryogenic electron microscopy, we demonstrate that mammalian (mouse) tissues contain three defined types of 'respirasome', supercomplexes made of CI, CIII and CIV. The stoichiometry and position of CIV differs in the three respirasomes, of which only one contains the supercomplex-associated factor SCAF1, whose involvement in respirasome formation has long been contended. Our structures confirm that the 'canonical' respirasome (the C-respirasome, CICIIICIV) does not contain SCAF1, which is instead associated to a different respirasome (the CS-respirasome), containing a second copy of CIV. We also identify an alternative respirasome (A-respirasome), with CIV bound to the 'back' of CI, instead of the 'toe'. This structural characterization of mouse mitochondrial supercomplexes allows us to hypothesize a mechanistic basis for their specific role in different metabolic conditions. #1: ![]() 年: 2018 タイトル: Real-space refinement in PHENIX for cryo-EM and crystallography 著者: Afonine PV / Adams PD | |||||||||
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構造の表示
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-EMDBアーカイブ
マップデータ | ![]() | 6.6 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 67.2 KB 67.2 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 21.1 KB | 表示 | ![]() |
画像 | ![]() | 52 KB | ||
Filedesc metadata | ![]() | 14.7 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8rgrMC ![]() 8pw5C ![]() 8pw6C ![]() 8pw7C ![]() 8rgpC ![]() 8rgqC ![]() 8rgtC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | composite map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 これらの図は立方格子座標系で作成されたものです | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Closed Complex I from murine liver
+超分子 #1: Closed Complex I from murine liver
+分子 #1: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
+分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
+分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
+分子 #4: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
+分子 #5: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
+分子 #6: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
+分子 #7: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
+分子 #8: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...
+分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
+分子 #10: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
+分子 #11: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
+分子 #12: Acyl carrier protein, mitochondrial
+分子 #13: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
+分子 #14: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
+分子 #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
+分子 #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
+分子 #17: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
+分子 #18: NADH-ubiquinone oxidoreductase chain 3
+分子 #19: NADH-ubiquinone oxidoreductase chain 1
+分子 #20: NADH-ubiquinone oxidoreductase chain 6
+分子 #21: NADH-ubiquinone oxidoreductase chain 4L
+分子 #22: NADH-ubiquinone oxidoreductase chain 5
+分子 #23: NADH-ubiquinone oxidoreductase chain 4
+分子 #24: NADH-ubiquinone oxidoreductase chain 2
+分子 #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...
+分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
+分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
+分子 #28: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
+分子 #29: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
+分子 #30: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
+分子 #31: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
+分子 #32: NADH dehydrogenase [ubiquinone] 1 subunit C2
+分子 #33: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
+分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
+分子 #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...
+分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...
+分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
+分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...
+分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
+分子 #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...
+分子 #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
+分子 #42: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
+分子 #43: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
+分子 #44: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
+分子 #45: IRON/SULFUR CLUSTER
+分子 #46: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #47: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
+分子 #48: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer
+分子 #49: FE2/S2 (INORGANIC) CLUSTER
+分子 #50: FLAVIN MONONUCLEOTIDE
+分子 #51: POTASSIUM ION
+分子 #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
+分子 #53: ZINC ION
+分子 #54: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...
+分子 #55: CARDIOLIPIN
+分子 #56: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE
+分子 #57: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.3 mg/mL | ||||||||||||
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緩衝液 | pH: 7.7 構成要素:
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グリッド | モデル: Quantifoil R0.6/1 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 1 | ||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 撮影したグリッド数: 1 / 実像数: 9453 / 平均露光時間: 4.4 sec. / 平均電子線量: 80.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 50.0 µm / 最大 デフォーカス(補正後): 2.5 µm / 最小 デフォーカス(補正後): 0.5 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.0 µm / 倍率(公称値): 81000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |