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- EMDB-16815: Tomogram of GBP1 coatomers assembled on brain polar lipid-derived... -

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Basic information

Entry
Database: EMDB / ID: EMD-16815
TitleTomogram of GBP1 coatomers assembled on brain polar lipid-derived small unilamellar vesicles.
Map dataElectron cryotomogram of GBP1 coatomers on BPLE-derived SUVs.
Sample
  • Organelle or cellular component: Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 dimers on brain polar lipid-derived SUVs.
KeywordsGBP1 / cryo-ET / liposome / coatomer / IMMUNE SYSTEM
Biological speciesHomo sapiens (human)
Methodelectron tomography / cryo EM
AuthorsKuhm TI / Jakobi AJ
Funding supportEuropean Union, Netherlands, 2 items
OrganizationGrant numberCountry
European Research Council (ERC)852880European Union
Netherlands Organisation for Scientific Research (NWO)NWO.STU.018-2.007 Netherlands
CitationJournal: To Be Published
Title: Structural basis of membrane targeting and coatomer formation by GBP1.
Authors: Kuhm TI / de Agrela Pinto C / Huber S / Taisne C / Giannopoulou EA / Pardon E / Steyaert J / Jakobi AJ
History
DepositionMar 9, 2023-
Header (metadata) releaseMar 27, 2024-
Map releaseMar 27, 2024-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16815.png

Downloads & links

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Map

FileDownload / File: emd_16815.map.gz / Format: CCP4 / Size: 1.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationElectron cryotomogram of GBP1 coatomers on BPLE-derived SUVs.
Voxel sizeX=Y=Z: 6.15 Å
Density
Minimum - Maximum-106.980419999999995 - 57.42351
Average (Standard dev.)1.4562379 (±3.611621)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions10101492197
Spacing14921010197
CellA: 9175.8 Å / B: 6211.5 Å / C: 1211.55 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_16815_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Mask #2

Fileemd_16815_msk_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 di...

EntireName: Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 dimers on brain polar lipid-derived SUVs.
Components
  • Organelle or cellular component: Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 dimers on brain polar lipid-derived SUVs.

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Supramolecule #1: Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 di...

SupramoleculeName: Membrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 dimers on brain polar lipid-derived SUVs.
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Chamber humidity: 98 % / Chamber temperature: 20 K / Instrument: LEICA EM GP / Details: Blotted for 4 seconds from the carbon side..
DetailsMembrane-assembled coatomer formed by GDP-AlF3-stabilised GBP1 dimers on brain polar lipid-derived SUVs.
SectioningOther: NO SECTIONING
Fiducial markerManufacturer: CMC Utrecht / Diameter: 10 nm

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Electron microscopy

MicroscopeJEOL 3200FSC
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 61 / Average exposure time: 3.0 sec. / Average electron dose: 1.64 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 12000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 4.1 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 5.0 µm
Sample stageSpecimen holder model: JEOL 3200FSC CRYOHOLDER / Cooling holder cryogen: NITROGEN

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Image processing

Final reconstructionAlgorithm: BACK PROJECTION / Resolution method: OTHER / Software - Name: IMOD / Number images used: 61

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