National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01AI147890
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
5U54AI150472-09
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
U54 AI170855-01
United States
Medical Research Council (MRC, United Kingdom)
MC_UP_1201/16
United Kingdom
Max Planck Society
Germany
Other private
RRID:SCR_021727
Other private
RRID:SCR_021740
National Science Foundation (NSF, United States)
DMR-1719875
United States
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2023 Title: Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM. Authors: Carolyn M Highland / Aaron Tan / Clifton L Ricaña / John A G Briggs / Robert A Dick / Abstract: The HIV-1 capsid houses the viral genome and interacts extensively with host cell proteins throughout the viral life cycle. It is composed of capsid protein (CA), which assembles into a conical ...The HIV-1 capsid houses the viral genome and interacts extensively with host cell proteins throughout the viral life cycle. It is composed of capsid protein (CA), which assembles into a conical fullerene lattice composed of roughly 200 CA hexamers and 12 CA pentamers. Previous structural analyses of individual CA hexamers and pentamers have provided valuable insight into capsid structure and function, but detailed structural information about these assemblies in the broader context of the capsid lattice is lacking. In this study, we combined cryoelectron tomography and single particle analysis (SPA) cryoelectron microscopy to determine structures of continuous regions of the capsid lattice containing both hexamers and pentamers. We also developed a method of liposome scaffold-based in vitro lattice assembly ("lattice templating") that enabled us to directly study the lattice under a wider range of conditions than has previously been possible. Using this approach, we identified a critical role for inositol hexakisphosphate in pentamer formation and determined the structure of the CA lattice bound to the capsid-targeting antiretroviral drug GS-6207 (lenacapavir). Our work reveals key structural details of the mature HIV-1 CA lattice and establishes the combination of lattice templating and SPA as a robust strategy for studying retroviral capsid structure and capsid interactions with host proteins and antiviral compounds.
Entire : HIV-1 CA protein capsid-like particles assembled in the presence ...
Entire
Name: HIV-1 CA protein capsid-like particles assembled in the presence of inositol hexakisphosphate
Components
Complex: HIV-1 CA protein capsid-like particles assembled in the presence of inositol hexakisphosphate
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Supramolecule #1: HIV-1 CA protein capsid-like particles assembled in the presence ...
Supramolecule
Name: HIV-1 CA protein capsid-like particles assembled in the presence of inositol hexakisphosphate type: complex / ID: 1 / Chimera: Yes / Parent: 0
Source (natural)
Organism: Escherichia coli (E. coli)
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Experimental details
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Structure determination
Method
cryo EM
Processing
subtomogram averaging
Aggregation state
particle
-
Sample preparation
Buffer
pH: 6.2 Component:
Concentration
Formula
Name
50.0 mM
MES
MES
2.5 mM
C6H18O24P6
Inositol hexakisphosphate
1.0 mM
C9H15O6P
Tris(2-carboxyethyl)phosphine
Grid
Model: C-flat-2/2 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR Details: The grid was glow discharged for 30 seconds at a current of 20 mA.
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
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Electron microscopy
Microscope
FEI TITAN KRIOS
Specialist optics
Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 3.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Applied symmetry - Point group: C5 (5 fold cyclic) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 6.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: subTOM / Number subtomograms used: 5244
Extraction
Number tomograms: 66 / Number images used: 5264 / Software - Name: MATLAB Details: Pentamer positions in HIV-1 CA protein capsid-like particles were identified by identifying patterns of 5 aligned hexamer positions corresponding to pentamer geometry using MATLAB.
Final angle assignment
Type: OTHER / Software - Name: subTOM
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