EMDB-16168: Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex...

Basic information

Entry

Database: EMDB / ID: EMD-16168

• Title: Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Complete composition)
• Map data: Composite density-modified map

Sample

• Complex: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete composition)
  • Protein or peptide: x 57 types
• Ligand: x 19 types

Function / homology

Function:

plastid outer membrane / TIM22 mitochondrial import inner membrane insertion complex / anther dehiscence / vegetative to reproductive phase transition of meristem / chloroplast outer membrane / mitochondrial processing peptidase / cold acclimation / plant-type cell wall / P450-containing electron transport chain / Lyases; Carbon-oxygen lyases; Hydro-lyases / NADH dehydrogenase complex / photorespiration / response to abscisic acid / protein insertion into mitochondrial inner membrane / embryo development ending in seed dormancy / respiratory chain complex III / plant-type vacuole / : / vacuole / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / regulation of reactive oxygen species metabolic process / response to osmotic stress / porin activity / plastid / cobalt ion binding / mitochondrial electron transport, ubiquinol to cytochrome c / ubiquinone binding / pore complex / protein homotrimerization / NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase activity / respiratory chain complex I / : / mitochondrial electron transport, NADH to ubiquinone / mitochondrial respiratory chain complex I assembly / electron transport coupled proton transport / acyl carrier activity / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / quinone binding / : / monoatomic ion transport / response to salt stress / aerobic respiration / respiratory electron transport chain / proton transmembrane transport / chloroplast / carbonate dehydratase activity / mitochondrial membrane / electron transport chain / metalloendopeptidase activity / mitochondrial intermembrane space / fatty acid biosynthetic process / 2 iron, 2 sulfur cluster binding / NAD binding / peroxisome / FMN binding / 4 iron, 4 sulfur cluster binding / mitochondrial outer membrane / carbohydrate metabolic process / mitochondrial inner membrane / electron transfer activity / oxidoreductase activity / mitochondrial matrix / copper ion binding / heme binding / nucleolus / protein homodimerization activity / mitochondrion / proteolysis / zinc ion binding / extracellular region / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / plasma membrane / cytosol

Domain/homology:

Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / Mitochondrial import inner membrane translocase subunit TIM22 / Putative NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / NADH-ubiquinone oxidoreductase 11kDa subunit / NADH-ubiquinone oxidoreductase 11 kDa subunit / At2g27730-like / : / : / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Adrenodoxin / NAD(P)H-quinone oxidoreductase subunit 3, bacterial/plastid / NAD(P)H-quinone oxidoreductase, subunit N/subunit 2 / Cytochrome b-c1 complex, subunit 6 / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit 9 superfamily / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome c1, transmembrane anchor, C-terminal / : / Cytochrome b / : / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / Cytochrome c1 / Cytochrome C1 family / : / Hexapeptide repeat / Cytochrome b/b6, C-terminal / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6 C-terminal region profile. / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / Rieske iron-sulphur protein, C-terminal / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / Tim17/Tim22/Tim23/Pmp24 family / Di-haem cytochrome, transmembrane / Bacterial transferase hexapeptide (six repeats) / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / Rieske iron-sulphur protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / : / Peptidase M16, C-terminal / Peptidase M16 inactive domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / Metalloenzyme, LuxS/M16 peptidase-like / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / Trimeric LpxA-like superfamily / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / : / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / SLBB domain / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8

Component:

Cytochrome b-c1 complex subunit 7-2, mitochondrial / NADH-ubiquinone oxidoreductase chain 2 / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / Outer envelope pore protein 16-3, chloroplastic/mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A / Acyl carrier protein 2, mitochondrial / NADH-ubiquinone oxidoreductase chain 5 / Cytochrome b / Acyl carrier protein 1, mitochondrial / NADH-ubiquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 4L / Cytochrome b-c1 complex subunit 6-1, mitochondrial / Probable mitochondrial-processing peptidase subunit beta, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial / At4g16450 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A / Uncharacterized protein At1g67785 / Transmembrane protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Excitatory amino acid transporter / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B / Cytochrome b-c1 complex subunit Rieske-1, mitochondrial / Cytochrome b-c1 complex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 / Gamma carbonic anhydrase 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / Cytochrome c1 2, heme protein, mitochondrial / Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / Gamma carbonic anhydrase 1, mitochondrial / At1g67350 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / Cytochrome b-c1 complex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / Cytochrome b-c1 complex subunit 8-1, mitochondrial / At2g31490 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein / Gamma carbonic anhydrase-like 2, mitochondrial / 2Fe-2S ferredoxin-like superfamily protein / At2g46540/F11C10.23 / Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial / Uncharacterized protein At2g27730, mitochondrial

• Biological species: Arabidopsis thaliana (thale cress) / thale cress (thale cress)
• Method: single particle reconstruction / cryo EM / Resolution: 2.09 Å
• Authors: Klusch N / Kuehlbrandt W

Funding support

Germany, 1 items

Organization	Grant number	Country
Max Planck Society		Germany

• Citation: Journal: Nat Plants / Year: 2023; Title: Cryo-EM structure of the respiratory I + III supercomplex from Arabidopsis thaliana at 2 Å resolution.; Authors: Niklas Klusch / Maximilian Dreimann / Jennifer Senkler / Nils Rugen / Werner Kühlbrandt / Hans-Peter Braun / ; Abstract: Protein complexes of the mitochondrial respiratory chain assemble into respiratory supercomplexes. Here we present the high-resolution electron cryo-microscopy structure of the Arabidopsis respiratory supercomplex consisting of complex I and a complex III dimer, with a total of 68 protein subunits and numerous bound cofactors. A complex I-ferredoxin, subunit B14.7 and P9, a newly defined subunit of plant complex I, mediate supercomplex formation. The component complexes stabilize one another, enabling new detailed insights into their structure. We describe (1) an interrupted aqueous passage for proton translocation in the membrane arm of complex I; (2) a new coenzyme A within the carbonic anhydrase module of plant complex I defining a second catalytic centre; and (3) the water structure at the proton exit pathway of complex III with a co-purified ubiquinone in the Q site. We propose that the main role of the plant supercomplex is to stabilize its components in the membrane.

History

Deposition	Nov 18, 2022	-
Header (metadata) release	Jan 25, 2023	-
Map release	Jan 25, 2023	-
Update	Feb 1, 2023	-
Current status	Feb 1, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_16168.map.gz / Format: CCP4 / Size: 1.6 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite density-modified map
• Voxel size: X=Y=Z: 0.573 Å

Density

Contour Level	By AUTHOR: 0.6
Minimum - Maximum	-12.511953 - 24.80058
Average (Standard dev.)	4.4749926e-05 (±0.15962407)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 750 750 750 Spacing 750 750 750
Cell	A=B=C: 429.75 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete ...

• Entire: Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete composition)

Components

• Complex: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete composition)
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
  • Protein or peptide: 2Fe-2S ferredoxin-like superfamily protein
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: Acyl carrier protein 1, mitochondrial
  • Protein or peptide: Acyl carrier protein 2, mitochondrial
  • Protein or peptide: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B
  • Protein or peptide: Outer envelope pore protein 16-3, chloroplastic/mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: At2g46540/F11C10.23
  • Protein or peptide: Transmembrane protein
  • Protein or peptide: Excitatory amino acid transporter
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B
  • Protein or peptide: At4g16450
  • Protein or peptide: ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein
  • Protein or peptide: At1g67350
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: AT2G31490 protein
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B
  • Protein or peptide: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: Uncharacterized protein At1g67785
  • Protein or peptide: Uncharacterized protein At2g27730, mitochondrial
  • Protein or peptide: Gamma carbonic anhydrase-like 2, mitochondrial
  • Protein or peptide: Gamma carbonic anhydrase 2, mitochondrial
  • Protein or peptide: Gamma carbonic anhydrase 1, mitochondrial
  • Protein or peptide: Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial
  • Protein or peptide: Probable mitochondrial-processing peptidase subunit beta, mitochondrial
  • Protein or peptide: Cytochrome b
  • Protein or peptide: Cytochrome b-c1 complex subunit Rieske-1, mitochondrial
  • Protein or peptide: Cytochrome c1 2, heme protein, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 7-2, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 8-1, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 6-1, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 9, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 10, mitochondrial
• Ligand: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE
• Ligand: IRON/SULFUR CLUSTER
• Ligand: PHOSPHATIDYLETHANOLAMINE
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: Ubiquinone-9
• Ligand: 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside
• Ligand: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE
• Ligand: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE
• Ligand: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE
• Ligand: FE (III) ION
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: ZINC ION
• Ligand: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate
• Ligand: CARDIOLIPIN
• Ligand: CROTONYL COENZYME A
• Ligand: PROTOPORPHYRIN IX CONTAINING FE
• Ligand: UBIQUINONE-7
• Ligand: water

Supramolecule #1: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete ...

• Supramolecule: Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Complete composition); type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#57
• Source (natural): Organism: Arabidopsis thaliana (thale cress)

Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 13.969396 KDa

Sequence

String:

(FME)MLEFAPIFI YLVISLLVSL ILLGVPFLFA SNSSTYPEKL SAYECGFDPF GDARSRFDIR FYLVSILFLI FDLEVT FFF PWAVSLNKID LFGFWSMMAF LFILTIGFLY EWKRGALDWE

Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 24.071949 KDa

Sequence

String:

MAMITRNTAT RLPLLLQSQR AVAAASVSHL HTSLPALSPS TSPTSYTRPG PPSTSPPPPG LSKAAEFVIS KVDDLMNWAR TGSIWPMTF GLACCAVEMM HTGAARYDLD RFGIIFRPSP RQSDCMIVAG TLTNKMAPAL RKVYDQMPEP RWVISMGSCA N GGGYYHYS YSVVRGCDRI VPVDIYVPGC PPTAEALLYG LLQLQKKINR RKDFLHWWNK

Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 22.91091 KDa

Sequence

String:

MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFLKLHTY TRVQVLIDIC GVDYPSRKRR FEVVYNLLST RYNSRIRVQ TSADEVTRIS SVVSLFPSAG WWEREVWDMF GVSFINHPDL RRILTDYGFE GHPLRKDFPL SGYVQVRYDD P EKRVVSEP IEMTQEFRYF DFASPWEQRS DG

Macromolecule #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 45.062922 KDa

Sequence

String:

MTTRKRQIKN FTLNFGPQHP AAHGVLRLVL EMNGEVVERA EPHIGLLHRG TEKLIEYKTY LQALPYFDRL DYVSMMAQEH AYSLAVEKL LNCEVPLRAQ YIRVLFCEIT RILNHLLALT THAMDVGALT PFLWAFEERE KLLEFYERVS GARMHASFIR P GGVAQDLP LGLCRDIDSF TQQFASRIDE LEEMLTGNRI WKQRLVDIGT VTAQQAKDWG FSGVMLRGSG VCWDLRRAAP YD VYDQLDF DVPVGTRGDC YDRYCIRIEE MRQSLRIIVQ CLNQMPSGMI KADDRKLCPP SRCRMKLSME SLIHHFELYT EGF SVPASS TYTAVEAPKG EFGVFLVSNG SNRPYRCKIR APGFAHLQGL DFMSKHHMLA DVVTIIGTQD IVFGEVDR

Macromolecule #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 28.423607 KDa

Sequence

String:

MLARLAAKRL LEIRQVFRQP TSQVTRSLST ALNYHLDSPD NKPDLPWEFS EANQSKVKEI LSYYPSNYKQ SAVIPLLDLA QQQNGGWLP VSAMNAVAKV IEVAPIRVYE VATFYSMFNR AKVGKYHLLV CGTTPCMIRG SRDIESALLD HLGVKRGEVT K DGLFSVGE MECMGCCVNA PMITVADYSN GSEGYTYNYF EDVTPEKVVE IVEKLRKGEK PPHGTQNPKR IKCGPEGGNK TL LGEPKPP QFRDLDAC

Macromolecule #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 53.522418 KDa

Sequence

String:

MAPVRGILGL QRAVSIWKES NRLTPALRSF STQAASTSTT PQPPPPPPPP EKTHFGGLKD EDRIFTNLYG LHDPFLKGAM KRGDWHRTK DLVLKGTDWI VNEMKKSGLR GRGGAGFPSG LKWSFMPKVS DGRPSYLVVN ADESEPGTCK DREIMRHDPH K LLEGCLIA GVGMRASAAY IYIRGEYVNE RLNLEKARRE AYAAGLLGKN ACGSGYDFEV YIHFGAGAYI CGEETALLES LE GKQGKPR LKPPFPANAG LYGCPTTVTN VETVAVSPTI LRRGPEWFSS FGRKNNAGTK LFCISGHVNK PCTVEEEMSI PLK ELIERH CGGVRGGWDN LLAIIPGGSS VPLIPKNICE DVLMDFDALK AVQSGLGTAA VIVMDKSTDV VDAIARLSYF YKHE SCGQC TPCREGTGWL WMIMERMKVG NAKLEEIDML QEVTKQIEGH TICALGDAAA WPVQGLIRHF RPELERRIRE RAERE LLQA AA

Macromolecule #7: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 81.619367 KDa

Sequence

String:

MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP TQILPPRNPV GGARVHFSNP EDAIEVFVDG YAVKVPKGF TVLQACEVAG VDIPRFCYHS RLSIAGNCRM CLVEVEKSPK PVASCAMPAL PGMKIKTDTP IAKKAREGVM E FLLMNHPL DCPICDQGGE CDLQDQSMAF GSDRGRFTEM KRSVVDKNLG PLVKTVMTRC IQCTRCVRFA SEVAGVQDLG IL GRGSGEE IGTYVEKLMT SELSGNVIDI CPVGALTSKP FAFKARNWEL KATETIDVSD AVGSNIRVDS RGPEVMRIIP RLN EDINEE WISDKTRFCY DGLKRQRLSD PMIRDSDGRF KAVSWRDALA VVGDIIHQVK PDEIVGVAGQ LSDAESMMVL KDFV NRMGS DNVWCEGTAA GVDADLRYSY LMNTSISGLE NADLFLLIGT QPRVEAAMVN ARICKTVRAS NAKVGYVGPP AEFNY DCKH LGTGPDTLKE IAEGRHPFCT ALKNAKNPAI IVGAGLFNRT DKNAILSSVE SIAQANNVVR PDWNGLNFLL QYAAQA AAL DLGLIQQSAK ALESAKFVYL MGADDVNVDK IPKDAFVVYQ GHHGDKAVYR ANVILPASAF TEKEGTYENT EGFTQQT VP AVPTVGDARD DWKIVRALSE VSGVKLPYNS IEGVRSRIKS VAPNLVHTDE REPAAFGPSL KPECKEAMST TPFQTVVE N FYMTNSITRA SKIMAQCSAV LLKKPFV

Macromolecule #8: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 36.02007 KDa

Sequence

String:

MYIAVPAEIL GIILPLLLGV AFLVLAERKV MAFVQRRKGP DVVGSFGLLQ PLADGLKLIL KEPISPSSAN FFLFRMAPVA TFMLSLVAW AVVPFDYGMV LSDLNIGLLY LFAISSLGVY GIIIAGRSSN SKYAFLGALR SAAQMVSYEV SIGLILITVL I CVGSCNLS EIVMAQKQIW FGIPLFPVLV MFFISCLAET NRAPFDLPEA EAELVAGYNV EYSSMGFALF FLGEYANMIL MS GLCTLFF LGGWLPILDL PIFKKIPGSI WFSIKVLFFL FLYIWVRAAF PRYRYDQLMG LGWKVFLPLS LAWVVSVSGL LVT FQWLP

Macromolecule #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 25.536801 KDa

Sequence

String:

MASILARRSL NTLRARHLVL SGQALQGSHL SRLQSRGISY GSNKDDEEAE QLSKEISKDW NTVFERSINT LFLTEMVRGL SLTLKYFFD PKVTINYPFE KGPLSPRFRG EHALRRYPTG EERCIACKLC EAVCPAQAIT IEAEEREDGS RRTTRYDIDM T KCIYCGFC QEACPVDAIV EGPNFEFATE THEELLYDKE KLLENGDRWE TEIAENLRSE SLYR

Macromolecule #10: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 23.690385 KDa

Sequence

String:

MILSVLSSLA LVSGLMVVRA KNPVHSVLFF ILVFCDTSGL LLLLGLDFFA MIFLVVYIGA IAVLFLFVVM MFHIQIAEIH EEVLRYLPV SGIIGLIFWW EMFFILDNES IPLLPTQRNT TSLRYTVYAG KVRSWTNLET LGNLLYTYYF VWFLVSSLIL L VAMIGAIV LTMHRTTKVK RQDVFRRNAI DFRRTIMRRT TDPLTIY

Macromolecule #11: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.193559 KDa

Sequence

String:

(FME)DLIKYFTFS MIIFILGIWG ILLNRRNILI MLMSIELMLL AVNLNFLVFS VSLDDMMGQV FALLVLTVAA AESAIG LAI FVITFRVRGT IAVEFINSIQ G

Macromolecule #12: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 74.497977 KDa

Sequence

String:

MYLLIVFLPL LGSSVAGFFG RFLGSEGSAI MTTTCVSFSS ILSLIAFYEV ALGASACYLR IAPWISSEMF DASWGFLFDS LTVVMLIVV TFISSLVHLY SISYMSEDPH SPRFMCYLSI FTFFMLMLVT GDNFLQLFLG WEGVGLASYL LIHFWFTRLQ A DKAAIKAM LVNRVGDFGL ALGILGCFTL FQTVDFSTIF ACASVPRNSW IFCNMRLNAI SLICILLFIG AVGKSAQIGL HT WLPDAME GPTPVSALIH AATMVTAGVF MIARCSPLFE YSPTALIVIT FAGAMTSFLA ATTGILQNDL KRVIAYSTCS QLG YMIFAC GISNYSVSVF HLMNHAFFKA LLFLSAGSVI HAMSDEQDMR KMGGLASSFP LTYAMMLIGS LSLIGFPFLT GFYS KDVIL ELAYTKYTIS GNFAFWLGSV SVLFTSYYSF RLLFLTFLVP TNSFGRDISR CHDAPIPMAI PLILLALGSL FVGYL AKDM MIGLGTNFWA NSLLVLPKNE ILAESEFAAP TIIKLIPILF STLGAFVAYN VNLVADQFQR AFQTSTFCNR LYSFFN KRW FFDQVLNDFL VRSFLRFGYE VSFEALDKGA IEILGPYGIS YTFRRLAERI SQLQSGFVYH YAFAMLLGLT LFVTFFC MW DSLSSWVDNR LSFILIVSSF YTKSSQE

Macromolecule #13: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 55.995664 KDa

Sequence

String:

MLEHFCECYF NLSGLILCPV LGSIILLFIP NSRIRLIRLI GLCASLITFL YSLVLWIQFD SSTAKFQFVE SLRWLPYENI NFYLGIDGI SLFFVILTTF LIPICILVGW SGMRSYGKEY IIAFLICEFL MIAVFCMLDL LLFYVFFESV LIPMFIIIGV W GSRQRKIK AAYQFFLYTL LGSLFMLLAI LLILFQTGTT DLQILLTTEF SERRQIFLWI AFFASFAVKV PMVPVHIWLP EA HVEAPTA GSVILAGILL KFGTYGFLRF SIPMFPEATL CFTPFIYTLS AIAIIYTSLT TLRQIDLKKI IAYSSVAHMN LVT IGMFSL NIQGIGGSIL LMLSHGLVSS ALFLCVGVLY DRHKTRLVRY YGGLVSTMPN FSTIFFFFTL ANMSLPGTSS FIGE FLILV GAFQRNSLVA TLAALGMILG AAYSLWLYNR VVSGNLKPDF LHKFSDLNGR EVFIFIPFLV GLVWMGVYPK VFLDC MHTS VSNLVQHGKF H

Macromolecule #14: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 55.486836 KDa

Sequence

String:

MKAEFVRILP HMFNLFLAVF PEIFIINATF ILLIHGVVFS TSKKYDYPPL ASNVGWLGLL SVLITLLLLA AGAPLLTIAH LFWNNLFRR DNFTYFCQIF LLLSTAGTIS MCFDFFDQER FDAFEFIVLI LLSTCGMLFM ISAYDLIAMY LAIELQSLCF Y VIAASKRK SEFSTEAGLK YLILGAFSSG ILLFGCSMIY GSTGATHFDQ LAKILTGYEI TGARSSGIFM GILFIAVGFL FK ITAVPFH MWAPDIYEGS PTPVTAFLSI APKISIFANI LRVFIYGSYG ATLQQIFFFC SIASMILGAL AAMAQTKVKR LLA YSSIGH VGYICIGFSC GTIEGIQSLL IGIFIYALMT MDAFAIVLAL RQTRVKYIAD LGALAKTNPI LAITFSITMF SYAG IPPLA GFCSKFYLFF AALGCGAYFL ALVGVVTSVI GCFYYIRLVK RMFFDTPRTW ILYEPMDRNK SLLLAMTSFF ITLFL LYPS PLFSVTHQMA LSLYL

Macromolecule #15: 2Fe-2S ferredoxin-like superfamily protein

• Macromolecule: Name: 2Fe-2S ferredoxin-like superfamily protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 17.626197 KDa

Sequence

String:

MATTLQKLSS QIHRLSPFTR SLIVRTSATS APSPSLGSKK VSDRIVKLSA IDPDGYKQDI IGLSGQTLLR ALTHTGLIDP ASHRLDDIE ACSAECEVQI AEEWLEKLPP RTYDEEYVLK RSSRSRILNK HSRLGCQVVL TQELQGMVVA VPEAKPWDIP

Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 43.988652 KDa

Sequence

String:

MQVVSRRLVQ RPLVGGASIY SSSSLRSLYG VSNHLNGTDN CRYSSSLATK GVGHLARKGT GGRSSVSGIV ATVFGATGFL GRYLVQQLA KMGSQVLVPF RGSEDSPRHL KLMGDLGQVV PMKFDPRDED SIKAVMAKAN VVINLIGREY ETRNFSFEDA N HHIAEKLA LVAKEHGGIM RYIQVSCLGA SVSSPSRMLR AKAAAEEAVL NALPEATIMR PATMIGTEDR ILNPWSMFVK KY GFLPLIG GGTTKFQPVY VVDVAAAIVA ALKDDGSSMG KTYELGGPDV FTTHELAEIM YDMIREWPRY VKLPFPIAKA MAA PRDFMV NKVPFPLPSP QIFNLDQINA LTTDTLVSDN ALKFQDLDLV PHKLKGYPVE FLIQYRKGGP NFGSTVSEKI PTDF YP

Macromolecule #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 17.160445 KDa

Sequence

String:

MALCATTQRT IRIAATLRRV ARPFATDAVV ESDYKRGEIG KVSGIPEEHL SRKVIIYSPA RTATQSGSGK LGKWKINFVS TLKWENPLM GWTSTGDPYA NVGDSALAFD SEEAAKSFAE RHGWDYKVKK PNTPLLKVKS YSDNFKWKGN PQPEN

Macromolecule #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 12.251122 KDa

Sequence

String:

MASNLLKALI RSQILPSSRR NFSVATTQLG IPTDDLVGNH TAKWMQDRSK KSPMELISEV PPIKVDGRIV ACEGDTNPAL GHPIEFICL DLNEPAICKY CGLRYVQDHH H

Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 10.865765 KDa

Sequence

String:

MAWRGSISKS MKELRILLCQ SSPASAPTRT FVEKNYKDLK SLNPKLPILI RECSGVQPQM WARYDMGVER CVNLDGLTEP QILKALENL VKSGATKA

Macromolecule #20: Acyl carrier protein 1, mitochondrial

• Macromolecule: Name: Acyl carrier protein 1, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 13.735628 KDa

Sequence

String:

MALRNAILRH LRVPVQTLGL NQSKIGFLGT IRSFSSHDDH LSREAVVDRV LDVVKSFPKV DPSKVTPEVH FQNDLGLDSL DTVEIVMAI EEEFKLEIPD KEADKIDSCS LAIEYVYNHP MSS

Macromolecule #21: Acyl carrier protein 2, mitochondrial

• Macromolecule: Name: Acyl carrier protein 2, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 14.183111 KDa

Sequence

String:

MAARGAMLRY LRVNVNPTIQ NPRECVLPFS ILLRRFSEEV RGSFLDKSEV TDRVLSVVKN FQKVDPSKVT PKANFQNDLG LDSLDSVEV VMALEEEFGF EIPDNEADKI QSIDLAVDFI ASHPQAK

Macromolecule #22: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subun...

• Macromolecule: Name: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 19.201906 KDa

Sequence

String:

MFLRAIGRPL LAKVKQTTGI VGLDVVPNAR AVLIDLYSKT LKEIQAVPED EGYRKAVESF TRQRLNVCKE EEDWEMIEKR LGCGQVEEL IEEARDELTL IGKMIEWDPW GVPDDYECEV IENDAPIPKH VPQHRPGPLP EQFYKTLEGL IAESKTEIPA A TPSDPQLK E

Macromolecule #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 15.102261 KDa

Sequence

String:

MAAPFALRKI GVPPNSANLT EARRRVFDFF RAACRSIPTI MDIYNLQDVV APSQLRYAIS AQIRNNAHIT DPKVIDLLIF KGMEELTDI VDHAKQRHHI IGQYVVGEGL VQNTGNKDQG KTDFLKNFYT SNYF

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.985954 KDa

Sequence

String:

MSSAVDATGN PIPTSAVLTA SAKHIGMRCM PENVAFLKCK KNDPNPEKCL DKGRDVTRCV LGLLKDLHQK CQKEMDDYVG CMYYYTNEF DLCRKEQEAF EKVCPLK

Macromolecule #25: Outer envelope pore protein 16-3, chloroplastic/mitochondrial

• Macromolecule: Name: Outer envelope pore protein 16-3, chloroplastic/mitochondrial; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 17.017348 KDa

Sequence

String:

MDPAEMRYLE EEDGPLMKTI KGSITGFGAG TIYGTILATW KDVPRVERNV ALPGLIRTLK MMGTHGLTFA AIGGVYIGVE QLVQNFRSK RDFYNGAIGG FVAGASVLGY RARSIPTAIA AGATLAVTSA LIDSGGQTTR VDNGREYYPY TVEKRAEADS

Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 16.145584 KDa

Sequence

String:

MTEAMIRNKP GMASVKDMPL LQDGPPPGGF APVRYARRIS NTGPSAMAMF LAVSGAFAWG MYQVGQGNKI RRALKEEKYA ARRTILPIL QAEEDERFVS EWKKYLEYEA DVMKDVPGWK VGENVYNSGR WMPPATGELR PDVW

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 7.349628 KDa

Sequence

String:

MSLVWLEAML PLGIIGGMLC IMGNSQYYIH KAYHGRPKHI GHDEWDVAME RRDKKVVEKA AAPSS

Macromolecule #28: At2g46540/F11C10.23

• Macromolecule: Name: At2g46540/F11C10.23 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 6.810177 KDa

Sequence

String:

MPVMEKLRMF VAQEPVVAAS CLIGGVGLFL PAVVRPILDS LEASKQVKAP PLTDVIAGVT GKKQS

Macromolecule #29: Transmembrane protein

• Macromolecule: Name: Transmembrane protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 9.876422 KDa

Sequence

String:

MGGGDHGHGA EGGDFRAKVW SMTGGPNCRP KHWRRNTAIA MFGVFLVCIP IAKLSAKLEQ RPHMPVRPIP SQIWCKNFGT KDDYEKEH

Macromolecule #30: Excitatory amino acid transporter

• Macromolecule: Name: Excitatory amino acid transporter / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 9.220749 KDa

Sequence

String:

MPISATMVGA LLGLGTQMYS NALRKLPYMR HPWEHVVGMG LGAVFANQLV KWDVKLKEDL DVMLAKARAA NERRYFDEDR D

Macromolecule #31: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 9.914133 KDa

Sequence

String:

MASGWGITGN KGRCYDFWMD FSECMSHCRE PKDCTLLRED YLECLHHSKE FQRRNRIYKE EQRKLRAASR KGEETGDGTH THH

Macromolecule #32: At4g16450

• Macromolecule: Name: At4g16450 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.383019 KDa

Sequence

String:

(FME)NTDITALEK AQYPVVDRNP AFTKVVGNFS TLDYLRFSTI TGISVTVGYL SGIKPGIKGP SMVTGGLIGL MGGFMY AYQ NSAGRLMGFF PNDGEVASYQ KRGGFSK

Macromolecule #33: ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein

• Macromolecule: Name: ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 12.648286 KDa

Sequence

String:

MPSTQSLTVA AKTLRNRIFS RSGSTSAGPS RWATPGHEER PKGYFMNRTP PAPGQSRKWE DWELPCYITS FLTIVILGVG LNAKPDLSI ETWAHQKALE RLEMEKLATA GDSSD

Macromolecule #34: At1g67350

• Macromolecule: Name: At1g67350 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.808244 KDa

Sequence

String:

MGFIMEFAEN LVLRLMENPE ERDRKAREHI YEMHERCKKI KEMWALPIRP YGFWTFERHN AQLRWDPQIS QVAGRRDPYD DLLEDNYTP PSSSSSSSD

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 7.58259 KDa

Sequence

String:

MGGGGHGGGI TYKGVTVHTP KTWHTVTGKG LCAVMWFWIL YRAKQDGPVV MGWRHPWDGH GDHGHGDHH

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 8.064313 KDa

Sequence

String:

MAKPLGTTGE FFRRRDEWRK HPMLSNQMRH ALPGIGIGVG AFCVYLVGEQ IYSKLMAPSS QSSHQKQPAP SH

Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 13.225222 KDa

Sequence

String:

MAGRLSGVAS RIMGGNGVVA RSVGSSLRQR AGMGLPVGKH IVPDKPLSVN DELMWDNGTA FPEPCIDRIA DTVGKYEALA WLSGGLGFF VGLGLLAVLN DKASKVPFTP RVYPYDNLRV ELGGEP

Macromolecule #38: AT2G31490 protein

• Macromolecule: Name: AT2G31490 protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 8.305539 KDa

Sequence

String:

MGGGMETNKN KFIEDWGSAR ENLEHNFRWT RRNFALIGIF GIALPIIVYK GIVKDFHMQD EDAGRPHRKF L

Macromolecule #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 13.638335 KDa

Sequence

String:

MSGVSTAAYF ARRAAQKERV RILYRRALKD TLNWAVHRHI FYRDASDLRE KFNVNQDVED VDRIDKLIAH GEAEYNKWRH PDPYIVPWA PGGSKFCRNP TPPAGIEIVY NYGLEDNP

Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.757832 KDa

Sequence

String:

MEVPGSSKKM IATQEEMSAA KIALGSRDMC AHLLIPLNKC RQAEFYLPWK CEDERHVYEK CEYELVMERM LAMKKIREEE ALAKQNKLQ GNAAVPLIPK TANA

Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 12.462276 KDa

Sequence

String:

MGRKKGLPEF EESAPDGFDP ENPYKDPVAM VEMREHIVRE KWIQIEKAKI LREKVKWCYR VEGVNHYQKC RHLVQQYLDS TRGVGWGKD HRPISLHGPK PEAVEAE

Macromolecule #42: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 18.346736 KDa

Sequence

String:

MALTVAKSAL EAIREKGLGG FMRMIREEGF MRCLPDGNLL QTKIHNIGAT LVGVDKFGNK YYQKLGDTQY GRHRWVEYAS KDRYNASQV PAEWHGWLHF ITDHTGDELL SLKPKRYGLE HKENFSGEGD AYIYHSKGHT LNPGQKNWTR YQSWVPTKTQ

Macromolecule #43: Uncharacterized protein At1g67785

• Macromolecule: Name: Uncharacterized protein At1g67785 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 7.54679 KDa

Sequence

String:

MVKVLTYFGM TLAAFAFWQS MDKVHVWIAL HQDEKQERME KEAEVRRVRA ELLRKAREED PLA

Macromolecule #44: Uncharacterized protein At2g27730, mitochondrial

• Macromolecule: Name: Uncharacterized protein At2g27730, mitochondrial / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 11.96552 KDa

Sequence

String:

MATRNALRIV SRRFSSGKVL SEEERAAENV FIKKMEQEKL QKLARQGPGE QAAGSASEAK VAGATASASA ESGPKVSEDK NRNYAVVAG VVAIVGSIGW YLKAGGKKQP EVQE

Macromolecule #45: Gamma carbonic anhydrase-like 2, mitochondrial

• Macromolecule: Name: Gamma carbonic anhydrase-like 2, mitochondrial / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 27.985113 KDa

Sequence

String:

MATSLARISK RSITSAVSSN LIRRYFAAEA VAVATTETPK PKSQVTPSPD RVKWDYRGQR QIIPLGQWLP KVAVDAYVAP NVVLAGQVT VWDGSSVWNG AVLRGDLNKI TVGFCSNVQE RCVVHAAWSS PTGLPAQTLI DRYVTVGAYS LLRSCTIEPE C IIGQHSIL MEGSLVETRS ILEAGSVLPP GRRIPSGELW GGNPARFIRT LTNEETLEIP KLAVAINHLS GDYFSEFLPY ST IYLEVEK FKKSLGIAI

Macromolecule #46: Gamma carbonic anhydrase 2, mitochondrial

• Macromolecule: Name: Gamma carbonic anhydrase 2, mitochondrial / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: Lyases; Carbon-oxygen lyases; Hydro-lyases
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 30.102207 KDa

Sequence

String:

MGTLGRAIYT VGNWIRGTGQ ALDRVGSLLQ GSHRIEEHLS RHRTLMNVFD KSPLVDKDVF VAPSASVIGD VQIGKGSSIW YGCVLRGDV NNISVGSGTN IQDNTLVHVA KTNISGKVLP TLIGDNVTVG HSAVIHGCTV EDDAFVGMGA TLLDGVVVEK H AMVAAGSL VKQNTRIPSG EVWGGNPAKF MRKLTDEEIV YISQSAKNYI NLAQIHASEN SKSFEQIEVE RALRKKYARK DE DYDSMLG ITRETPPELI LPDNVLPGGK PVAKVPSTQY F

Macromolecule #47: Gamma carbonic anhydrase 1, mitochondrial

• Macromolecule: Name: Gamma carbonic anhydrase 1, mitochondrial / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO / EC number: Lyases; Carbon-oxygen lyases; Hydro-lyases
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 30.010039 KDa

Sequence

String:

MGTLGRAFYS VGFWIRETGQ ALDRLGCRLQ GKNYFREQLS RHRTLMNVFD KAPIVDKEAF VAPSASVIGD VHIGRGSSIW YGCVLRGDV NTVSVGSGTN IQDNSLVHVA KSNLSGKVHP TIIGDNVTIG HSAVLHGCTV EDETFIGMGA TLLDGVVVEK H GMVAAGAL VRQNTRIPSG EVWGGNPARF LRKLTDEEIA FISQSATNYS NLAQAHAAEN AKPLNVIEFE KVLRKKHALK DE EYDSMLG IVRETPPELN LPNNILPDKE TKRPSNVN

Macromolecule #48: Probable mitochondrial-processing peptidase subunit alpha-1, mito...

• Macromolecule: Name: Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial; type: protein_or_peptide / ID: 48 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 54.459766 KDa

Sequence

String:

MYRTAASRAR ALKGVLTRSL RPARYASSSA VAETSSSTPA YLSWLSGGSR AALTSLDMPL QGVSLPPPLA DKVEPSKLQI TTLPNGLKI ASETTPNPAA SIGLYVDCGS IYEAPYFHGA THLLERMAFK STLNRTHFRL VREIEAIGGN TSASASREQM S YTIDALKT YVPEMVEVLI DSVRNPAFLD WEVNEELRKM KVEIAELAKN PMGFLLEAIH SAGYSGPLAS PLYAPESALD RL NGELLEE FMTENFTAAR MVLAASGVEH EELLKVAEPL TSDLPNVPPQ LAPKSQYVGG DFRQHTGGEA THFAVAFEVP GWN NEKEAV TATVLQMLMG GGGSFSAGGP GKGMHSWLYR RVLNEYQEVQ SCTAFTSIFN DTGLFGIYGC SSPQFAAKAI ELAA KELKD VAGGKVNQAH LDRAKAATKS AVLMNLESRM IAAEDIGRQI LTYGERKPVD QFLKSVDQLT LKDIADFTSK VISKP LTMG SFGDVLAVPS YDTISSKFR

Macromolecule #49: Probable mitochondrial-processing peptidase subunit beta, mitocho...

• Macromolecule: Name: Probable mitochondrial-processing peptidase subunit beta, mitochondrial; type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO / EC number: mitochondrial processing peptidase
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 59.23475 KDa

Sequence

String:

MAMKNLLSLA RRSQRRLFLT QATRSSSSFS AIDSVPASAS PTALSPPPPH LMPYDHAAEI IKNKIKKLEN PDKRFLKYAS PHPILASHN HILSAPETRV TTLPNGLRVA TESNLSAKTA TVGVWIDAGS RFESDETNGT AHFLEHMIFK GTDRRTVRAL E EEIEDIGG HLNAYTSREQ TTYYAKVLDS NVNQALDVLA DILQNSKFEE QRINRERDVI LREMQEVEGQ TDEVVLDHLH AT AFQYTPL GRTILGPAQN VKSITREDLQ NYIKTHYTAS RMVIAAAGAV KHEEVVEQVK KLFTKLSSDP TTTSQLVANE PAS FTGSEV RMIDDDLPLA QFAVAFEGAS WTDPDSVALM VMQTMLGSWN KNVGGGKHVG SDLTQRVAIN EIAESIMAFN TNYK DTGLF GVYAVAKADC LDDLSYAIMY EVTKLAYRVS DADVTRARNQ LKSSLLLHMD GTSPIAEDIG RQLLTYGRRI PTAEL FARI DAVDASTVKR VANKYIYDKD IAISAIGPIQ DLPDYNKFRR RTYWNRY

Macromolecule #50: Cytochrome b

• Macromolecule: Name: Cytochrome b / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 44.310609 KDa

Sequence

String:

MTIRNQRFSL LKQPISSTLN QHLVDYPTPS NLSYWWGFGS LAGICLVIQI VTGVFLAMHY TPHVDLAFNS VEHIMRDVEG GWLLRYMHA NGASMFFIVV YLHIFRGLYY ASYSSPREFV WCLGVVIFLL MIVTAFIGYV LPWGQMSFWG ATVITSLASA I PVVGDTIV TWLWGGFSVD NATLNRFFSL HYLLPFILVG ASLLHLAALH QYGSNNPLGV HSEMDKIAFY PYFYVKDLVG WV AFAIFFS IWIFYAPNVL GHPDNYIPAN PMSTPPHIVP EWYFLPIYAI LRSIPDKAGG VAAIALVFIC LLALPFFKSM YVR SSSFRP IYQGMFWLLL ADCLLLGWIG CQPVEAPFVT IGQISSLVFF LFFAITPILG RVGRGIPNSY TDETDHT

Macromolecule #51: Cytochrome b-c1 complex subunit Rieske-1, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit Rieske-1, mitochondrial; type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO / EC number: quinol-cytochrome-c reductase
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 29.645584 KDa

Sequence

String:

MLRVAGRRLF SVSQRSSTAT SFVVSRDHTL SDGGGDSSSA PRSLPSADLS SYHRSLIRGF SSQVLAQGNE IGFGSEVPAT VEAVKTPNS KIVYDDHNHE RYPPGDPSKR AFAYFVLSGG RFVYASVLRL LVLKLIVSMS ASKDVLALAS LEVDLGSIEP G TTVTVKWR GKPVFIRRRT EDDIKLANSV DVGSLRDPQE DSVRVKNPEW LVVVGVCTHL GCIPLPNAGD YGGWFCPCHG SH YDISGRI RKGPAPYNLE VPTYSFLEEN KLLIG

Macromolecule #52: Cytochrome c1 2, heme protein, mitochondrial

• Macromolecule: Name: Cytochrome c1 2, heme protein, mitochondrial / type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 33.728238 KDa

Sequence

String:

MVGGGVIRQL LRRKLHSQSV ATPVLSWLSS KKANEDAGSA GLRAFALMGA GITGLLSFST VASADEAEHG LECPNYPWPH EGILSSYDH ASIRRGHQVY QQVCASCHSM SLISYRDLVG VAYTEEEAKA MAAEIEVVDG PNDEGEMFTR PGKLSDRLPE P YSNESAAR FANGGAYPPD LSLVTKARHN GQNYVFALLT GYRDPPAGIS IREGLHYNPY FPGGAIAMPK MLNDEAVEYE DG TPATEAQ MGKDVVSFLS WAAEPEMEER KLMGFKWIFL LSLALLQAAY YRRLKWSVLK SRKLVLDVVN

Macromolecule #53: Cytochrome b-c1 complex subunit 7-2, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 7-2, mitochondrial / type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 14.620021 KDa

Sequence

String:

MASFLQRLVD PRKNFLARMH MKSVSNRLRR YGLRYDDLYD PLYDLDIKEA LNRLPREIVD ARNQRLMRAM DLSMKHEYLP DNLQAVQTP FRSYLQDMLA LVKRERAERE ALGALPLYQR TIP

Macromolecule #54: Cytochrome b-c1 complex subunit 8-1, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 8-1, mitochondrial / type: protein_or_peptide / ID: 54 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 8.517977 KDa

Sequence

String:

MGKQPVKLKA VVYALSPFQQ KIMTGLWKDL PEKIHHKVSE NWISATLLVT PVVGTYWYAQ YFKEQEKLEH RF

Macromolecule #55: Cytochrome b-c1 complex subunit 6-1, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 6-1, mitochondrial / type: protein_or_peptide / ID: 55 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 7.997315 KDa

Sequence

String:

MADDEVVDPK KYLEESCKPK CVKPLLEYQA CVKRIQGDDS GHKHCTGQYF DYWQCIDKCV APKLFAKLK

Macromolecule #56: Cytochrome b-c1 complex subunit 9, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 9, mitochondrial / type: protein_or_peptide / ID: 56 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 8.461634 KDa

Sequence

String:

MEYAARRNQK GAFEGFYKLI MRRNSVYVTF IIAGAFFGER AVDYGVHKLW ERNNVGKRYE DISVLGQRPV EE

Macromolecule #57: Cytochrome b-c1 complex subunit 10, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 10, mitochondrial / type: protein_or_peptide / ID: 57 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: thale cress (thale cress)
• Molecular weight: Theoretical: 5.980947 KDa

Sequence

String:

MAGTSGLLNA VKPKIQTIDI QAAAGWGIAA AAGAIWVVQP FGWIKKTFID PPPTEEK

Macromolecule #58: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-...

• Macromolecule: Name: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE; type: ligand / ID: 58 / Number of copies: 10 / Formula: PGT
• Molecular weight: Theoretical: 751.023 Da

Chemical component information

ChemComp-PGT:
(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipid*YM

Macromolecule #59: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 59 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #60: PHOSPHATIDYLETHANOLAMINE

• Macromolecule: Name: PHOSPHATIDYLETHANOLAMINE / type: ligand / ID: 60 / Number of copies: 14 / Formula: PTY
• Molecular weight: Theoretical: 734.039 Da

Chemical component information

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

Macromolecule #61: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 61 / Number of copies: 4 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #62: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 62 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #63: Ubiquinone-9

• Macromolecule: Name: Ubiquinone-9 / type: ligand / ID: 63 / Number of copies: 1 / Formula: UQ9
• Molecular weight: Theoretical: 795.226 Da

Chemical component information

ChemComp-UQ9:
Ubiquinone-9

Macromolecule #64: 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl...

• Macromolecule: Name: 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside; type: ligand / ID: 64 / Number of copies: 6 / Formula: Q7G
• Molecular weight: Theoretical: 1.165315 KDa

Chemical component information

ChemComp-Q7G:
2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside

Macromolecule #65: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

• Macromolecule: Name: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / type: ligand / ID: 65 / Number of copies: 11 / Formula: 3PH
• Molecular weight: Theoretical: 704.998 Da

Chemical component information

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

Macromolecule #66: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5...

• Macromolecule: Name: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE; type: ligand / ID: 66 / Number of copies: 9 / Formula: PC7
• Molecular weight: Theoretical: 763.1 Da

Chemical component information

ChemComp-PC7:
(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipid*YM

Macromolecule #67: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14...

• Macromolecule: Name: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE; type: ligand / ID: 67 / Number of copies: 4 / Formula: UQ5
• Molecular weight: Theoretical: 522.758 Da

Chemical component information

ChemComp-UQ5:
2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE

Macromolecule #68: FE (III) ION

• Macromolecule: Name: FE (III) ION / type: ligand / ID: 68 / Number of copies: 1 / Formula: FE
• Molecular weight: Theoretical: 55.845 Da

Macromolecule #69: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 69 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #70: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 70 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #71: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

• Macromolecule: Name: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate; type: ligand / ID: 71 / Number of copies: 2 / Formula: 8Q1
• Molecular weight: Theoretical: 540.651 Da

Chemical component information

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

Macromolecule #72: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 72 / Number of copies: 9 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Macromolecule #73: CROTONYL COENZYME A

• Macromolecule: Name: CROTONYL COENZYME A / type: ligand / ID: 73 / Number of copies: 1 / Formula: COO
• Molecular weight: Theoretical: 835.608 Da

Chemical component information

ChemComp-COO:
CROTONYL COENZYME A

Macromolecule #74: PROTOPORPHYRIN IX CONTAINING FE

• Macromolecule: Name: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 74 / Number of copies: 6 / Formula: HEM
• Molecular weight: Theoretical: 616.487 Da

Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

Macromolecule #75: UBIQUINONE-7

• Macromolecule: Name: UBIQUINONE-7 / type: ligand / ID: 75 / Number of copies: 1 / Formula: UQ7
• Molecular weight: Theoretical: 658.992 Da

Chemical component information

ChemComp-UQ7:
UBIQUINONE-7

Macromolecule #76: water

• Macromolecule: Name: water / type: ligand / ID: 76 / Number of copies: 4834 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.18 mg/mL
• Buffer: pH: 7.4
• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: CONTINUOUS / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: GRAPHENE / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 215000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1215138

Startup model

Type of model: EMDB MAP
EMDB ID:

11878

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.09 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1.3) / Number images used: 213993
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.3)

Atomic model buiding 1

• Software: Name: Coot (ver. 0.9.5)
• Refinement: Space: REAL

Output model

PDB-8bpx:
Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Complete composition)