ジャーナル: Proc Natl Acad Sci U S A / 年: 2008 タイトル: Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail. 著者: Ye Xiang / Marc C Morais / Daniel N Cohen / Valorie D Bowman / Dwight L Anderson / Michael G Rossmann / 要旨: The small bacteriophage phi29 must penetrate the approximately 250-A thick external peptidoglycan cell wall and cell membrane of the Gram-positive Bacillus subtilis, before ejecting its dsDNA genome ...The small bacteriophage phi29 must penetrate the approximately 250-A thick external peptidoglycan cell wall and cell membrane of the Gram-positive Bacillus subtilis, before ejecting its dsDNA genome through its tail into the bacterial cytoplasm. The tail of bacteriophage phi29 is noncontractile and approximately 380 A long. A 1.8-A resolution crystal structure of gene product 13 (gp13) shows that this tail protein has spatially well separated N- and C-terminal domains, whose structures resemble lysozyme-like enzymes and metallo-endopeptidases, respectively. CryoEM reconstructions of the WT bacteriophage and mutant bacteriophages missing some or most of gp13 shows that this enzyme is located at the distal end of the phi29 tail knob. This finding suggests that gp13 functions as a tail-associated, peptidoglycan-degrading enzyme able to cleave both the polysaccharide backbone and peptide cross-links of the peptidoglycan cell wall. Comparisons of the gp13(-) mutants with the phi29 mature and emptied phage structures suggest the sequence of events that occur during the penetration of the tail through the peptidoglycan layer.
名称: Bacteriophage phi29 mutant sus13(330) / タイプ: sample / ID: 1000 集合状態: capsid protein forms t3 q5 prolate icosahedron Number unique components: 9
分子量
理論値: 35.2 MDa
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超分子 #1: Bacillus phage phi29
超分子
名称: Bacillus phage phi29 / タイプ: virus / ID: 1 詳細: Fibered bacteriophage phi29 mutant sus13(330). A large fragment of gene product 13 could be detected in the mutant particles. NCBI-ID: 10756 / 生物種: Bacillus phage phi29 / ウイルスタイプ: VIRUS-LIKE PARTICLE / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: No / ウイルス・中空状態: No