EMDB-14204: Proteasome-ZFAND5 Complex Z+D state

Basic information

Entry

Database: EMDB / ID: EMD-14204

• Title: Proteasome-ZFAND5 Complex Z+D state
• Map data: ZFAND5-proteasome complex Z D state

Sample

• Complex: human proteasome zfand5 complex Z+D state
  • Protein or peptide: x 27 types
• Protein or peptide: x 6 types
• Ligand: x 4 types

• Keywords: proteasome / ZFAND5 / Activation / STRUCTURAL PROTEIN

Function / homology

Function:

Impaired BRCA2 translocation to the nucleus / Impaired BRCA2 binding to SEM1 (DSS1) / thyrotropin-releasing hormone receptor binding / modulation by host of viral transcription / meiosis I / Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases / smooth muscle tissue development / proteasome accessory complex / integrator complex / purine ribonucleoside triphosphate binding / fibroblast migration / respiratory system process / proteasome regulatory particle / positive regulation of proteasomal protein catabolic process / cytosolic proteasome complex / proteasome regulatory particle, lid subcomplex / proteasome-activating activity / vasculature development / proteasome regulatory particle, base subcomplex / metal-dependent deubiquitinase activity / negative regulation of programmed cell death / regulation of endopeptidase activity / skeletal system morphogenesis / protein K63-linked deubiquitination / Homologous DNA Pairing and Strand Exchange / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Regulation of ornithine decarboxylase (ODC) / proteasome core complex / Resolution of D-loop Structures through Holliday Junction Intermediates / Cross-presentation of soluble exogenous antigens (endosomes) / : / K63-linked deubiquitinase activity / Somitogenesis / Impaired BRCA2 binding to RAD51 / myofibril / immune system process / proteasome binding / transcription factor binding / regulation of protein catabolic process / face development / proteasome storage granule / Presynaptic phase of homologous DNA pairing and strand exchange / blastocyst development / platelet-derived growth factor receptor signaling pathway / general transcription initiation factor binding / polyubiquitin modification-dependent protein binding / NF-kappaB binding / endopeptidase activator activity / protein deubiquitination / proteasome assembly / proteasome endopeptidase complex / proteasome core complex, beta-subunit complex / proteasome core complex, alpha-subunit complex / threonine-type endopeptidase activity / mRNA export from nucleus / enzyme regulator activity / regulation of proteasomal protein catabolic process / inclusion body / sarcomere / proteasome complex / Regulation of activated PAK-2p34 by proteasome mediated degradation / ciliary basal body / proteolysis involved in protein catabolic process / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / SCF-beta-TrCP mediated degradation of Emi1 / Asymmetric localization of PCP proteins / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / stem cell differentiation / Vpu mediated degradation of CD4 / Assembly of the pre-replicative complex / Degradation of DVL / negative regulation of inflammatory response to antigenic stimulus / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Dectin-1 mediated noncanonical NF-kB signaling / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / lipopolysaccharide binding / Hh mutants are degraded by ERAD / Degradation of AXIN / Activation of NF-kappaB in B cells / Degradation of GLI1 by the proteasome / Hedgehog ligand biogenesis / Defective CFTR causes cystic fibrosis / G2/M Checkpoints / Negative regulation of NOTCH4 signaling / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Autodegradation of the E3 ubiquitin ligase COP1 / Vif-mediated degradation of APOBEC3G / double-strand break repair via homologous recombination / P-body / Hedgehog 'on' state / Regulation of RUNX3 expression and activity / Degradation of GLI2 by the proteasome

Domain/homology:

: / Zinc finger, AN1-type / AN1-like Zinc finger / AN1-like Zinc finger / Zinc finger AN1-type profile. / AN1-like Zinc finger / Zinc finger, A20-type / A20-like zinc finger / Zinc finger A20-type profile. / A20-like zinc fingers / : / Ubiquitin interaction motif / : / 26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix / 26S proteasome non-ATPase regulatory subunit Rpn12 / 26S proteasome regulatory subunit, C-terminal / Proteasome regulatory subunit C-terminal / DSS1/SEM1 / 26S proteasome regulatory subunit RPN5, C-terminal domain / : / : / DSS1/SEM1 family / 26S proteasome regulatory subunit RPN5 C-terminal domain / 26S proteasome subunit RPN2, N-terminal domain / PSD13 N-terminal repeats / DSS1_SEM1 / 26S proteasome regulatory subunit Rpn6, N-terminal / 6S proteasome subunit Rpn6, C-terminal helix domain / 26S proteasome regulatory subunit RPN6 N-terminal domain / 26S proteasome subunit RPN6 C-terminal helix domain / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit / 26S Proteasome non-ATPase regulatory subunit 13 / 26S proteasome regulatory subunit RPN2, C-terminal / 26S proteasome regulatory subunit RPN2 C-terminal domain / 26S Proteasome non-ATPase regulatory subunit 7/8 / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn1 subunit / RPN1, N-terminal / 26S proteasome non-ATPase regulatory subunit RPN1, C-terminal / : / RPN1 N-terminal domain / 26S proteasome non-ATPase regulatory subunit RPN1 C-terminal / 26S proteasome regulatory subunit 7, OB domain / : / : / PSMD12/CSN4, N-terminal / 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 / 26S proteasome regulatory subunit Rpn7, N-terminal / 26S proteasome subunit RPN7 / 26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4 / Proteasome/cyclosome repeat / Proteasome/cyclosome repeat / Ubiquitin-interacting motif. / PCI/PINT associated module / : / von Willebrand factor type A domain / Proteasome subunit alpha 1 / HEAT repeats / Proteasomal ATPase OB C-terminal domain / Proteasomal ATPase OB C-terminal domain / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Ubiquitin interacting motif / Ubiquitin-interacting motif (UIM) domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Proteasome beta subunit, C-terminal / Proteasome beta subunits C terminal / Proteasome subunit beta 4 / Proteasome subunit beta 2 / Proteasome beta 3 subunit / Proteasome subunit alpha6 / Proteasome subunit alpha5 / Proteasome beta-type subunits signature. / Peptidase T1A, proteasome beta-subunit / Proteasome beta-type subunit, conserved site / Proteasome subunit A N-terminal signature / Proteasome alpha-type subunits signature. / Proteasome alpha-subunit, N-terminal domain / Proteasome subunit A N-terminal signature Add an annotation / von Willebrand factor (vWF) type A domain / : / Proteasome alpha-type subunit / Proteasome alpha-type subunit profile. / VWFA domain profile. / Proteasome B-type subunit / Proteasome beta-type subunit profile. / Proteasome subunit / Proteasome, subunit alpha/beta / von Willebrand factor, type A / AAA ATPase, AAA+ lid domain / AAA+ lid domain / ATPase, AAA-type, conserved site / AAA-protein family signature. / JAB/MPN domain

Component:

Proteasome 26S subunit, ATPase 6 / 26S proteasome non-ATPase regulatory subunit 11 / 26S proteasome non-ATPase regulatory subunit 12 / 26S proteasome non-ATPase regulatory subunit 14 / Proteasome subunit alpha type-7 / 26S proteasome non-ATPase regulatory subunit 3 / AN1-type zinc finger protein 5 / 26S proteasome regulatory subunit 6A / Proteasome subunit beta type-1 / Proteasome subunit alpha type-1 / Proteasome subunit alpha type-2 / Proteasome subunit alpha type-3 / Proteasome subunit alpha type-4 / Proteasome subunit alpha type-5 / Proteasome subunit beta type-4 / Proteasome subunit beta type-6 / Proteasome subunit beta type-5 / 26S proteasome regulatory subunit 7 / 26S proteasome regulatory subunit 6B / 26S proteasome non-ATPase regulatory subunit 8 / Proteasome subunit beta type-3 / Proteasome subunit beta type-2 / 26S proteasome non-ATPase regulatory subunit 7 / 26S proteasome non-ATPase regulatory subunit 4 / 26S proteasome complex subunit SEM1 / Proteasome subunit alpha type-6 / 26S proteasome regulatory subunit 4 / 26S proteasome regulatory subunit 8 / 26S proteasome non-ATPase regulatory subunit 2 / 26S proteasome non-ATPase regulatory subunit 6 / Proteasome subunit beta type-7 / 26S proteasome non-ATPase regulatory subunit 1 / 26S proteasome non-ATPase regulatory subunit 13

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 4.1 Å
• Authors: Zhu Y / Lu Y

Funding support

1 items

Organization	Grant number	Country
Not funded

• Citation: Journal: Mol Cell / Year: 2023; Title: Molecular mechanism for activation of the 26S proteasome by ZFAND5.; Authors: Donghoon Lee / Yanan Zhu / Louis Colson / Xiaorong Wang / Siyi Chen / Emre Tkacik / Lan Huang / Qi Ouyang / Alfred L Goldberg / Ying Lu / ; Abstract: Various hormones, kinases, and stressors (fasting, heat shock) stimulate 26S proteasome activity. To understand how its capacity to degrade ubiquitylated proteins can increase, we studied mouse ZFAND5, which promotes protein degradation during muscle atrophy. Cryo-electron microscopy showed that ZFAND5 induces large conformational changes in the 19S regulatory particle. ZFAND5's AN1 Zn-finger domain interacts with the Rpt5 ATPase and its C terminus with Rpt1 ATPase and Rpn1, a ubiquitin-binding subunit. Upon proteasome binding, ZFAND5 widens the entrance of the substrate translocation channel, yet it associates only transiently with the proteasome. Dissociation of ZFAND5 then stimulates opening of the 20S proteasome gate. Using single-molecule microscopy, we showed that ZFAND5 binds ubiquitylated substrates, prolongs their association with proteasomes, and increases the likelihood that bound substrates undergo degradation, even though ZFAND5 dissociates before substrate deubiquitylation. These changes in proteasome conformation and reaction cycle can explain the accelerated degradation and suggest how other proteasome activators may stimulate proteolysis.

History

Deposition	Jan 27, 2022	-
Header (metadata) release	Feb 8, 2023	-
Map release	Feb 8, 2023	-
Update	Oct 16, 2024	-
Current status	Oct 16, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_14204.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: ZFAND5-proteasome complex Z D state
• Voxel size: X=Y=Z: 1.37 Å

Density

Contour Level	By AUTHOR: 0.0328
Minimum - Maximum	-0.0583596 - 0.12418681
Average (Standard dev.)	0.0010785713 (±0.006963986)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 320 320 320 Spacing 320 320 320
Cell	A=B=C: 438.4 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : human proteasome zfand5 complex Z+D state

• Entire: Name: human proteasome zfand5 complex Z+D state

Components

• Complex: human proteasome zfand5 complex Z+D state
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 1
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 3
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 12
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 11
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 6
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 7
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 13
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 4
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 8
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 2
  • Protein or peptide: Isoform 2 of 26S proteasome regulatory subunit 8
  • Protein or peptide: 26S proteasome complex subunit SEM1
  • Protein or peptide: Proteasome subunit alpha type-6
  • Protein or peptide: Proteasome subunit alpha type-2
  • Protein or peptide: Proteasome subunit alpha type-4
  • Protein or peptide: Proteasome subunit alpha type-7
  • Protein or peptide: Proteasome subunit alpha type-5
  • Protein or peptide: Isoform Long of Proteasome subunit alpha type-1
  • Protein or peptide: Proteasome subunit alpha type-3
  • Protein or peptide: Proteasome subunit beta type-6
  • Protein or peptide: Proteasome subunit beta type-7
  • Protein or peptide: Proteasome subunit beta type-3
  • Protein or peptide: Proteasome subunit beta type-2
  • Protein or peptide: Proteasome subunit beta type-5
  • Protein or peptide: Proteasome subunit beta type-1
  • Protein or peptide: Proteasome subunit beta type-4
  • Protein or peptide: AN1-type zinc finger protein 5
• Protein or peptide: 26S proteasome non-ATPase regulatory subunit 14
• Protein or peptide: 26S protease regulatory subunit 7
• Protein or peptide: 26S proteasome regulatory subunit 4
• Protein or peptide: 26S protease regulatory subunit 6B
• Protein or peptide: 26S proteasome regulatory subunit 10B
• Protein or peptide: 26S protease regulatory subunit 6A
• Ligand: ZINC ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-DIPHOSPHATE

Supramolecule #1: human proteasome zfand5 complex Z+D state

• Supramolecule: Name: human proteasome zfand5 complex Z+D state / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8, #10-#11, #14, #18-#33
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 26S proteasome non-ATPase regulatory subunit 1

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 105.958234 KDa

Sequence

String:

MITSAAGIIS LLDEDEPQLK EFALHKLNAV VNDFWAEISE SVDKIEVLYE DEGFRSRQFA ALVASKVFYH LGAFEESLNY ALGAGDLFN VNDNSEYVET IIAKCIDHYT KQCVENADLP EGEKKPIDQR LEGIVNKMFQ RCLDDHKYKQ AIGIALETRR L DVFEKTIL ESNDVPGMLA YSLKLCMSLM QNKQFRNKVL RVLVKIYMNL EKPDFINVCQ CLIFLDDPQA VSDILEKLVK ED NLLMAYQ ICFDLYESAS QQFLSSVIQN LRTVGTPIAS VPGSTNTGTV PGSEKDSDSM ETEEKTSSAF VGKTPEASPE PKD QTLKMI KILSGEMAIE LHLQFLIRNN NTDLMILKNT KDAVRNSVCH TATVIANSFM HCGTTSDQFL RDNLEWLARA TNWA KFTAT ASLGVIHKGH EKEALQLMAT YLPKDTSPGS AYQEGGGLYA LGLIHANHGG DIIDYLLNQL KNASNDIVRH GGSLG LGLA AMGTARQDVY DLLKTNLYQD DAVTGEAAGL ALGLVMLGSK NAQAIEDMVG YAQETQHEKI LRGLAVGIAL VMYGRM EEA DALIESLCRD KDPILRRSGM YTVAMAYCGS GNNKAIRRLL HVAVSDVNDD VRRAAVESLG FILFRTPEQC PSVVSLL SE SYNPHVRYGA AMALGICCAG TGNKEAINLL EPMTNDPVNY VRQGALIASA LIMIQQTEIT CPKVNQFRQL YSKVINDK H DDVMAKFGAI LAQGILDAGG HNVTISLQSR TGHTHMPSVV GVLVFTQFWF WFPLSHFLSL AYTPTCVIGL NKDLKMPKV QYKSNCKPST FAYPAPLEVP KEKEKEKVST AVLSITAKAK KKEKEKEKKE EEKMEVDEAE KKEEKEKKKE PEPNFQLLDN PARVMPAQL KVLTMPETCR YQPFKPLSIG GIIILKDTSE DIEELVEPVA AHGPKIEEEE QEPEPPEPFE YIDD

UniProtKB: 26S proteasome non-ATPase regulatory subunit 1

Macromolecule #2: 26S proteasome non-ATPase regulatory subunit 3

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.935297 KDa

Sequence

String:

KQEGSARRRG ADKAKPPPGG GEQEPPPPPA PQDVEMKEEA ATGGGSTGEA DGKTAAAAAE HSQRELDTVT LEDIKEHVKQ LEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL P EVEAYLQL LVVIFMMNSK RYKEAQKISD DLMQKISTQN RRALDLVAAK CYYYHARVYE FLDKLDVVRS FLHARLRTAT LR HDADGQA TLLNLLLRNY LHYSLYDQAE KLVSKSVFPE QANNNEWARY LYYTGRIKAI QLEYSEARRT MTNALRKAPQ HTA VGFKQT VHKLLIVVEL LLGEIPDRLQ FRQPSLKRSL MPYFLLTQAV RTGNLAKFNQ VLDQFGEKFQ ADGTYTLIIR LRHN VIKTG VRMISLSYSR ISLADIAQKL QLDSPEDAEF IVAKAIRDGV IEASINHEKG YVQSKEMIDI YSTREPQLAF HQRIS FCLD IHNMSVKAMR FPPKSYNKDL ESAEERRERE QQDLEFAKEM AEDDDDSFP

UniProtKB: 26S proteasome non-ATPase regulatory subunit 3

Macromolecule #3: 26S proteasome non-ATPase regulatory subunit 12

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 12 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.979359 KDa

Sequence

String:

MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIML LSKRRSQLKQ AVAKMVQQCC TYVEEITDLP IKLRLIDTLR MVTEGKIYVE IERARLTKTL ATIKEQNGDV K EAASILQE LQVETYGSME KKERVEFILE QMRLCLAVKD YIRTQIISKK INTKFFQEEN TEKLKLKYYN LMIQLDQHEG SY LSICKHY RAIYDTPCIQ AESEKWQQAL KSVVLYVILA PFDNEQSDLV HRISGDKKLE EIPKYKDLLK LFTTMELMRW STL VEDYGM ELRKGSLESP ATDVFGSTEE GEKRWKDLKN RVVEHNIRIM AKYYTRITMK RMAQLLDLSV DESEAFLSNL VVNK TIFAK VDRLAGIINF QRPKDPNNLL NDWSQKLNSL MSLVNKTTHL IAKEEMIHNL Q

UniProtKB: 26S proteasome non-ATPase regulatory subunit 12

Macromolecule #4: 26S proteasome non-ATPase regulatory subunit 11

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 11 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.526688 KDa

Sequence

String:

MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLV RSLLDLFLDM EAATGQEVEL CLECIEWAKS EKRTFLRQAL EARLVSLYFD TKRYQEALHL GSQLLRELKK M DDKALLVE VQLLESKTYH ALSNLPKARA ALTSARTTAN AIYCPPKLQA TLDMQSGIIH AAEEKDWKTA YSYFYEAFEG YD SIDSPKA ITSLKYMLLC KIMLNTPEDV QALVSGKLAL RYAGRQTEAL KCVAQASKNR SLADFEKALT DYRAELRDDP IIS THLAKL YDNLLEQNLI RVIEPFSRVQ IEHISSLIKL SKADVERKLS QMILDKKFHG ILDQGEGVLI IFDEPPVDKT YEAA LETIQ NMSKVVDSLY NKAKKLT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 11

Macromolecule #5: 26S proteasome non-ATPase regulatory subunit 6

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 45.592285 KDa

Sequence

String:

MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNM APYYEALCKS LDWQIDVDLL NKMKKANEDE LKRLDEELE DAEKNLGESE IRDAMMAKAE YLCRIGDKEG ALTAFRKTYD KTVALGHRLD IVFYLLRIGL FYMDNDLITR N TEKAKSLI EEGGDWDRRN RLKVYQGLYC VAIRDFKQAA ELFLDTVSTF TSYELMDYKT FVTYTVYVSM IALERPDLRE KV IKGAEIL EVLHSLPAVR QYLFSLYECR YSVFFQSLAV VEQEMKKDWL FAPHYRYYVR EMRIHAYSQL LESYRSLTLG YMA EAFGVG VEFIDQELSR FIAAGRLHCK IDKVNEIVET NRPDSKNWQY QETIKKGDLL LNRVQKLSRV INM

UniProtKB: 26S proteasome non-ATPase regulatory subunit 6

Macromolecule #6: 26S proteasome non-ATPase regulatory subunit 7

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.086441 KDa

Sequence

String:

MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE DDKDDSVWFL DHDYLENMYG MFKKVNARE RIVGWYHTGP KLHKNDIAIN ELMKRYCPNS VLVIIDVKPK DLGLPTEAYI SVEEVHDDGT PTSKTFEHVT S EIGAEEAE EVGVEHLLRD IKDTTVGTLS QRITNQVHGL KGLNSKLLDI RSYLEKVATG KLPINHQIIY QLQDVFNLLP DV SLQEFVK AFYLKTNDQM VVVYLASLIR SVVALHNLIN NKIANRDAEK KEGQEKEESK KDRKEDKEKD KDKEKSDVKK EEK KEKK

UniProtKB: 26S proteasome non-ATPase regulatory subunit 7

Macromolecule #7: 26S proteasome non-ATPase regulatory subunit 13

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 42.995359 KDa

Sequence

String:

MKDVPGFLQQ SQNSGPGQPA VWHRLEELYT KKLWHQLTLQ VLDFVQDPCF AQGDGLIKLY ENFISEFEHR VNPLSLVEII LHVVRQMTD PNVALTFLEK TREKVKSSDE AVILCKTAIG ALKLNIGDLQ VTKETIEDVE EMLNNLPGVT SVHSRFYDLS S KYYQTIGN HASYYKDALR FLGCVDIKDL PVSEQQERAF TLGLAGLLGE GVFNFGELLM HPVLESLRNT DRQWLIDTLY AF NSGNVER FQTLKTAWGQ QPDLAANEAQ LLRKIQLLCL MEMTFTRPAN HRQLTFEEIA KSAKITVNEV ELLVMKALSV GLV KGSIDE VDKRVHMTWV QPRVLDLQQI KGMKDRLEFW CTDVKSMEML VEHQAHDILT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 13

Macromolecule #8: 26S proteasome non-ATPase regulatory subunit 4

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 40.78159 KDa

Sequence

String:

MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTG IRVAHLALKH RQGKNHKMRI IAFVGSPVED NEKDLVKLAK RLKKEKVNVD IINFGEEEVN TEKLTAFVNT L NGKDGTGS HLVTVPPGPS LADALISSPI LAGEGGAMLG LGASDFEFGV DPSADPELAL ALRVSMEEQR QRQEEEARRA AA ASAAEAG IATTGTEDSD DALLKMTISQ QEFGRTGLPD LSSMTEEEQI AYAMQMSLQG AEFGQAESAD IDASSAMDTS EPA KEEDDY DVMQDPEFLQ SVLENLPGVD PNNEAIRNAM GSLASQATKD GKKDKKEEDK K

UniProtKB: 26S proteasome non-ATPase regulatory subunit 4

Macromolecule #9: 26S proteasome non-ATPase regulatory subunit 14

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO; EC number: Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.488824 KDa

Sequence

String:

DRLLRLGGGM PGLGQGPPTD APAVDTAEQV YISSLALLKM LKHGRAGVPM EVMGLMLGEF VDDYTVRVID VFAMPQSGTG VSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF R LINANMMV LGHEPRQTTS NLGHLNKPSI QALIHGLNRH YYSITINYRK NELEQKMLLN LHKKSWMEGL TLQDYSEHCK HN ESVVKEM LELAKNYNKA VEEEDKMTPE QLAIKNVGKQ DPKRHLEEHV DVLMTSNIVQ CLAAMLDTVV FK

UniProtKB: 26S proteasome non-ATPase regulatory subunit 14

Macromolecule #10: 26S proteasome non-ATPase regulatory subunit 8

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.536676 KDa

Sequence

String:

FIKGRAPRAP PRERRRATRG GLRQVVAPPR ALGSTSRPHF RRASVCRRRC RKSGGLLAAS RKMAAAAVNG AAGFSSSGPA ATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD I PSFERYMA QLKCYYFDYK EQLPESAYMH QLLGLNLLFL LSQNRVAEFH TELERLPAKD IQTNVYIKHP VSLEQYLMEG SY NKVFLAK GNIPAESYTF FIDILLDTIR DEIAGCIEKA YEKILFTEAT RILFFNTPKK MTDYAKKRGW VLGPNNYYSF ASQ QQKPED TTIPSTELAK QVIEYARQLE MIV

UniProtKB: 26S proteasome non-ATPase regulatory subunit 8

Macromolecule #11: 26S proteasome non-ATPase regulatory subunit 2

• Macromolecule: Name: 26S proteasome non-ATPase regulatory subunit 2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.313625 KDa

Sequence

String:

MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSM TSVPKPLKFL RPHYGKLKEI YENMAPGENK RFAADIISVL AMTMSGEREC LKYRLVGSQE ELASWGHEYV R HLAGEVAK EWQELDDAEK VQREPLLTLV KEIVPYNMAH NAEHEACDLL MEIEQVDMLE KDIDENAYAK VCLYLTSCVN YV PEPENSA LLRCALGVFR KFSRFPEALR LALMLNDMEL VEDIFTSCKD VVVQKQMAFM LGRHGVFLEL SEDVEEYEDL TEI MSNVQL NSNFLALARE LDIMEPKVPD DIYKTHLENN RFGGSGSQVD SARMNLASSF VNGFVNAAFG QDKLLTDDGN KWLY KNKDH GMLSAAASLG MILLWDVDGG LTQIDKYLYS SEDYIKSGAL LACGIVNSGV RNECDPALAL LSDYVLHNSN TMRLG SIFG LGLAYAGSNR EDVLTLLLPV MGDSKSSMEV AGVTALACGM IAVGSCNGDV TSTILQTIME KSETELKDTY ARWLPL GLG LNHLGKGEAI EAILAALEVV SEPFRSFANT LVDVCAYAGS GNVLKVQQLL HICSEHFDSK EKEEDKDKKE KKDKDKK EA PADMGAHQGV AVLGIALIAM GEEIGAEMAL RTFGHLLRYG EPTLRRAVPL ALALISVSNP RLNILDTLSK FSHDADPE V SYNSIFAMGM VGSGTNNARL AAMLRQLAQY HAKDPNNLFM VRLAQGLTHL GKGTLTLCPY HSDRQLMSQV AVAGLLTVL VSFLDVRNII LGKSHYVLYG LVAAMQPRML VTFDEELRPL PVSVRVGQAV DVVGQAGKPK TITGFQTHTT PVLLAHGERA ELATEEFLP VTPILEGFVI LRKNPNYDL

UniProtKB: 26S proteasome non-ATPase regulatory subunit 2

Macromolecule #12: 26S protease regulatory subunit 7

• Macromolecule: Name: 26S protease regulatory subunit 7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 48.700805 KDa

Sequence

String:

MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQS EQPLQVARCT KIINADSEDP KYIINVKQFA KFVVDLSDQV APTDIEEGMR VGVDRNKYQI HIPLPPKIDP T VTMMQVEE KPDVTYSDVG GCKEQIEKLR EVVETPLLHP ERFVNLGIEP PKGVLLFGPP GTGKTLCARA VANRTDACFI RV IGSELVQ KYVGEGARMV RELFEMARTK KACLIFFDEI DAIGGARFDD GAGGDNEVQR TMLELINQLD GFDPRGNIKV LMA TNRPDT LDPALMRPGR LDRKIEFSLP DLEGRTHIFK IHARSMSVER DIRFELLARL CPNSTGAEIR SVCTEAGMFA IRAR RKIAT EKDFLEAVNK VIKSYAKFSA TPRYMTYN

UniProtKB: 26S proteasome regulatory subunit 7

Macromolecule #13: 26S proteasome regulatory subunit 4

• Macromolecule: Name: 26S proteasome regulatory subunit 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 48.429586 KDa

Sequence

String:

MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLE EKQEEERSKV DDLRGTPMSV GTLEEIIDDN HAIVSTSVGS EHYVSILSFV DKDLLEPGCS VLLNHKVHAV I GVLMDDTD PLVTVMKVEK APQETYADIG GLDNQIQEIK ESVELPLTHP EYYEEMGIKP PKGVILYGPP GTGKTLLAKA VA NQTSATF LRVVGSELIQ KYLGDGPKLV RELFRVAEEH APSIVFIDEI DAIGTKRYDS NSGGEREIQR TMLELLNQLD GFD SRGDVK VIMATNRIET LDPALIRPGR IDRKIEFPLP DEKTKKRIFQ IHTSRMTLAD DVTLDDLIMA KDDLSGADIK AICT EAGLM ALRERRMKVT NEDFKKSKEN VLYKKQE

UniProtKB: 26S proteasome regulatory subunit 4

Macromolecule #14: Isoform 2 of 26S proteasome regulatory subunit 8

• Macromolecule: Name: Isoform 2 of 26S proteasome regulatory subunit 8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.852121 KDa

Sequence

String:

MELEEGKAGS GLRQYYLSKI EELQLIVNDK SQNLRRLQAQ RNELNAKVRL LREELQLLQE QGSYVGEVVR AMDKKKVLVK VHPEGKFVV DVDKNIDIND VTPNCRVALR NDSYTLHKIL PNKVDPLVSL MMVEKVPDST YEMIGGLDKQ IKEIKEVIEL P VKHPELFE ALGIAQPKGV LLYGPPGTGK TLLARAVAHH TDCTFIRVSG SELVQKFIGE GARMVRELFV MAREHAPSII FM DEIDSIG SSRLEGGSGG DSEVQRTMLE LLNQLDGFEA TKNIKVIMAT NRIDILDSAL LRPGRIDRKI EFPPPNEEAR LDI LKIHSR KMNLTRGINL RKIAELMPGA SGAEVKGVCT EAGMYALRER RVHVTQEDFE MAVAKVMQKD SEKNMSIKKL WK

UniProtKB: 26S proteasome regulatory subunit 8

Macromolecule #15: 26S protease regulatory subunit 6B

• Macromolecule: Name: 26S protease regulatory subunit 6B / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.426141 KDa

Sequence

String:

MEEIGILVEK AQDEIPALSV SRPQTGLSFL GPEPEDLEDL YSRYKKLQQE LEFLEVQEEY IKDEQKNLKK EFLHAQEEVK RIQSIPLVI GQFLEAVDQN TAIVGSTTGS NYYVRILSTI DRELLKPNAS VALHKHSNAL VDVLPPEADS SIMMLTSDQK P DVMYADIG GMDIQKQEVR EAVELPLTHF ELYKQIGIDP PRGVLMYGPP GCGKTMLAKA VAHHTTAAFI RVVGSEFVQK YL GEGPRMV RDVFRLAKEN APAIIFIDEI DAIATKRFDA QTGADREVQR ILLELLNQMD GFDQNVNVKV IMATNRADTL DPA LLRPGR LDRKIEFPLP DRRQKRLIFS TITSKMNLSE EVDLEDYVAR PDKISGADIN SICQESGMLA VRENRYIVLA KDFE KAYKT VIKKDEQEHE FYK

UniProtKB: 26S proteasome regulatory subunit 6B

Macromolecule #16: 26S proteasome regulatory subunit 10B

• Macromolecule: Name: 26S proteasome regulatory subunit 10B / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 45.867027 KDa

Sequence

String:

MAIPGIPYER RLLIMADPRD KALQDYRKKL LEHKEIDGRL KELREQLKEL TKQYEKSEND LKALQSVGQI VGEVLKQLTE EKFIVKATN GPRYVVGCRR QLDKSKLKPG TRVALDMTTL TIMRYLPREV DPLVYNMSHE DPGNVSYSEI GGLSEQIREL R EVIELPLT NPELFQRVGI IPPKGCLLYG PPGTGKTLLA RAVASQLDCN FLKVVSSSIV DKYIGESARL IREMFNYARD HQ PCIIFMD EIDAIGGRRF SEGTSADREI QRTLMELLNQ MDGFDTLHRV KMIMATNRPD TLDPALLRPG RLDRKIHIDL PNE QARLDI LKIHAGPITK HGEIDYEAIV KLSDGFNGAD LRNVCTEAGM FAIRADHDFV VQEDFMKAVR KVADSKKLES KLDY KPV

UniProtKB: Proteasome 26S subunit, ATPase 6

Macromolecule #17: 26S protease regulatory subunit 6A

• Macromolecule: Name: 26S protease regulatory subunit 6A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.266457 KDa

Sequence

String:

MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYL VSNVIELLDV DPNDQEEDGA NIDLDSQRKG KCAVIKTSTR QTYFLPVIGL VDAEKLKPGD LVGVNKDSYL I LETLPTEY DSRVKAMEVD ERPTEQYSDI GGLDKQIQEL VEAIVLPMNH KEKFENLGIQ PPKGVLMYGP PGTGKTLLAR AC AAQTKAT FLKLAGPQLV QMFIGDGAKL VRDAFALAKE KAPSIIFIDE LDAIGTKRFD SEKAGDREVQ RTMLELLNQL DGF QPNTQV KVIAATNRVD ILDPALLRSG RLDRKIEFPM PNEEARARIM QIHSRKMNVS PDVNYEELAR CTDDFNGAQC KAVC VEAGM IALRRGATEL THEDYMEGIL EVQAKKKANL QYYA

UniProtKB: 26S proteasome regulatory subunit 6A

Macromolecule #18: 26S proteasome complex subunit SEM1

• Macromolecule: Name: 26S proteasome complex subunit SEM1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.284611 KDa

Sequence

String:

MSEKKQPVDL GLLEEDDEFE EFPAEDWAGL DEDEDAHVWE DNWDDDNVED DFSNQLRAEL EKHGYKMETS

UniProtKB: 26S proteasome complex subunit SEM1

Macromolecule #19: Proteasome subunit alpha type-6

• Macromolecule: Name: Proteasome subunit alpha type-6 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.301262 KDa

Sequence

String:

SRGSSAGFDR HITIFSPEGR LYQVEYAFKA INQGGLTSVA VRGKDCAVIV TQKKVPDKLL DSSTVTHLFK ITENIGCVMT GMTADSRSQ VQRARYEAAN WKYKYGYEIP VDMLCKRIAD ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ VYKCDPAGYY C GFKATAAG VKQTESTSFL EKKVKKKFDW TFEQTVETAI TCLSTVLSID FKPSEIEVGV VTVENPKFRI LTEAEIDAHL VA LAERD

UniProtKB: Proteasome subunit alpha type-6

Macromolecule #20: Proteasome subunit alpha type-2

• Macromolecule: Name: Proteasome subunit alpha type-2 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.796338 KDa

Sequence

String:

AERGYSFSLT TFSPSGKLVQ IEYALAAVAG GAPSVGIKAA NGVVLATEKK QKSILYDERS VHKVEPITKH IGLVYSGMGP DYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA T AMGKNYVN GKTFLEKRYN EDLELEDAIH TAILTLKESF EGQMTEDNIE VGICNEAGFR RLTPTEVKDY LAAIA

UniProtKB: Proteasome subunit alpha type-2

Macromolecule #21: Proteasome subunit alpha type-4

• Macromolecule: Name: Proteasome subunit alpha type-4 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.394648 KDa

Sequence

String:

SRRYDSRTTI FSPEGRLYQV EYAMEAIGHA GTCLGILAND GVLLAAERRN IHKLLDEVFF SEKIYKLNED MACSVAGITS DANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK A TCIGNNSA AAVSMLKQDY KEGEMTLKSA LALAIKVLNK TMDVSKLSAE KVEIATLTRE NGKTVIRVLK QKEVEQLIKK HE EEEAKAE REKKEKEQKE KDK

UniProtKB: Proteasome subunit alpha type-4

Macromolecule #22: Proteasome subunit alpha type-7

• Macromolecule: Name: Proteasome subunit alpha type-7 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.798695 KDa

Sequence

String:

SYDRAITVFS PDGHLFQVEY AQEAVKKGST AVGVRGRDIV VLGVEKKSVA KLQDERTVRK ICALDDNVCM AFAGLTADAR IVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA I GRGAKSVR EFLEKNYTDE AIETDDLTIK LVIKALLEVV QSGGKNIELA VMRRDQSLKI LNPEEIEKYV AEIEKEKEEN EK KKQKKAS

UniProtKB: Proteasome subunit alpha type-7

Macromolecule #23: Proteasome subunit alpha type-5

• Macromolecule: Name: Proteasome subunit alpha type-5 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.304779 KDa

Sequence

String:

FLTRSEYDRG VNTFSPEGRL FQVEYAIEAI KLGSTAIGIQ TSEGVCLAVE KRITSPLMEP SSIEKIVEID AHIGCAMSGL IADAKTLID KARVETQNHW FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG PQLFHMDPSG T FVQCDARA IGSASEGAQS SLQEVYHKSM TLKEAIKSSL IILKQVMEEK LNATNIELAT VQPGQNFHMF TKEELEEVIK DI

UniProtKB: Proteasome subunit alpha type-5

Macromolecule #24: Isoform Long of Proteasome subunit alpha type-1

• Macromolecule: Name: Isoform Long of Proteasome subunit alpha type-1 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.150277 KDa

Sequence

String:

QLSKVKFRNQ YDNDVTVWSP QGRIHQIEYA MEAVKQGSAT VGLKSKTHAV LVALKRAQSE LAAHQKKILH VDNHIGISIA GLTADARLL CNFMRQECLD SRFVFDRPLP VSRLVSLIGS KTQIPTQRYG RRPYGVGLLI AGYDDMGPHI FQTCPSANYF D CRAMSIGA RSQSARTYLE RHMSEFMECN LNELVKHGLR ALRETLPAEQ DLTTKNVSIG IVGKDLEFTI YDDDDVSPFL EG LEERPQR KAQPAQPADE PAEKADEPME H

UniProtKB: Proteasome subunit alpha type-1

Macromolecule #25: Proteasome subunit alpha type-3

• Macromolecule: Name: Proteasome subunit alpha type-3 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.338057 KDa

Sequence

String:

SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG Y WGCAIGKA RQAAKTEIEK LQMKEMTCRD IVKEVAKIIY IVHDEVKDKA FELELSWVGE LTNGRHEIVP KDIREEAEKY AK ESLKEED ESDDDNM

UniProtKB: Proteasome subunit alpha type-3

Macromolecule #26: Proteasome subunit beta type-6

• Macromolecule: Name: Proteasome subunit beta type-6 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.246455 KDa

Sequence

String:

AATLLAARGA GPAPAWGPEA FTPDWESREV STGTTIMAVQ FDGGVVLGAD SRTTTGSYIA NRVTDKLTPI HDRIFCCRSG SAADTQAVA DAVTYQLGFH SIELNEPPLV HTAASLFKEM CYRYREDLMA GIIIAGWDPQ EGGQVYSVPM GGMMVRQSFA I GGSGSSYI YGYVDATYRE GMTKEECLQF TANALALAME RDGSSGGVIR LAAIAESGVE RQVLLGDQIP KFAVATLPPA

UniProtKB: Proteasome subunit beta type-6

Macromolecule #27: Proteasome subunit beta type-7

• Macromolecule: Name: Proteasome subunit beta type-7 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.869223 KDa

Sequence

String:

AAVSVYAPPV GGFSFDNCRR NAVLEADFAK RGYKLPKVRK TGTTIAGVVY KDGIVLGADT RATEGMVVAD KNCSKIHFIS PNIYCCGAG TAADTDMTTQ LISSNLELHS LSTGRLPRVV TANRMLKQML FRYQGYIGAA LVLGGVDVTG PHLYSIYPHG S TDKLPYVT MGSGSLAAMA VFEDKFRPDM EEEEAKNLVS EAIAAGIFND LGSGSNIDLC VISKNKLDFL RPYTVPNKKG TR LGRYRCE KGTTAVLTEK ITPLEIEVLE ETVQTMDTS

UniProtKB: Proteasome subunit beta type-7

Macromolecule #28: Proteasome subunit beta type-3

• Macromolecule: Name: Proteasome subunit beta type-3 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.841701 KDa

Sequence

String:

SIMSYNGGAV MAMKGKNCVA IAADRRFGIQ AQMVTTDFQK IFPMGDRLYI GLAGLATDVQ TVAQRLKFRL NLYELKEGRQ IKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP D HLFETISQ AMLNAVDRDA VSGMGVIVHI IEKDKITTRT LKARMD

UniProtKB: Proteasome subunit beta type-3

Macromolecule #29: Proteasome subunit beta type-2

• Macromolecule: Name: Proteasome subunit beta type-2 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.864277 KDa

Sequence

String:

MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLA DCLRSRTPYH VNLLLAGYDE HEGPALYYMD YLAALAKAPF AAHGYGAFLT LSILDRYYTP TISRERAVEL L RKCLEELQ KRFILNLPTF SVRIIDKNGI HDLDNISFPK QGS

UniProtKB: Proteasome subunit beta type-2

Macromolecule #30: Proteasome subunit beta type-5

• Macromolecule: Name: Proteasome subunit beta type-5 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.379053 KDa

Sequence

String:

ALASVLERPL PVNQRGFFGL GGRADLLDLG PGSLSDGLSL AAPGWGVPEE PGIEMLHGTT TLAFKFRHGV IVAADSRATA GAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI C GWDKRGPG LYYVDSEGNR ISGATFSVGS GSVYAYGVMD RGYSYDLEVE QAYDLARRAI YQATYRDAYS GGAVNLYHVR ED GWIRVSS DNVADLHEKY SGSTP

UniProtKB: Proteasome subunit beta type-5

Macromolecule #31: Proteasome subunit beta type-1

• Macromolecule: Name: Proteasome subunit beta type-1 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.391201 KDa

Sequence

String:

LSSTAMYSAP GRDLGMEPHR AAGPLQLRFS PYVFNGGTIL AIAGEDFAIV ASDTRLSEGF SIHTRDSPKC YKLTDKTVIG CSGFHGDCL TLTKIIEARL KMYKHSNNKA MTTGAIAAML STILYSRRFF PYYVYNIIGG LDEEGKGAVY SFDPVGSYQR D SFKAGGSA SAMLQPLLDN QVGFKNMQNV EHVPLSLDRA MRLVKDVFIS AAERDVYTGD ALRICIVTKE GIREETVSLR KD

UniProtKB: Proteasome subunit beta type-1

Macromolecule #32: Proteasome subunit beta type-4

• Macromolecule: Name: Proteasome subunit beta type-4 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.099986 KDa

Sequence

String:

EAFLGSRSGL WAGGPAPGQF YRIPSTPDSF MDPASALYRG PITRTQNPMV TGTSVLGVKF EGGVVIAADM LGSYGSLARF RNISRIMRV NNSTMLGASG DYADFQYLKQ VLGQMVIDEE LLGDGHSYSP RAIHSWLTRA MYSRRSKMNP LWNTMVIGGY A DGESFLGY VDMLGVAYEA PSLATGYGAY LAQPLLREVL EKQPVLSQTE ARDLVERCMR VLYYRDARSY NRFQIATVTE KG VEIEGPL STETNWDIAH MISGFE

UniProtKB: Proteasome subunit beta type-4

Macromolecule #33: AN1-type zinc finger protein 5

• Macromolecule: Name: AN1-type zinc finger protein 5 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.160172 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAQETNQTPG PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QQNSGRMSPM GTASGSNSPT SDSASIQRAE ASLNNCEGAA GSTSEKSRN VPVAALPVTQ QMTEMSISRE DKITTPKTEV SEPVVTQPSP SVSQPSTSQS EEKAPELPKP KKNRCFMCRK K VGLTGFDC RCGNLFCGLH RYSDKHNCPY DYKAEAAAKI RKENPVVVAE KIQRI

UniProtKB: AN1-type zinc finger protein 5

Macromolecule #34: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #35: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 3 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #36: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 3 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #37: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 37 / Number of copies: 2 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 2 mg/mL
• Buffer: pH: 7
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 46.6 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 28928
• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 28928
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD