EMDB-14114: Structure of the human 48S initiation complex in closed state (h4...

Basic information

Entry

Database: EMDB / ID: EMD-14114

• Title: Structure of the human 48S initiation complex in closed state (h48S AUG closed)

Sample

• Complex: Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA
  • Protein or peptide: x 48 types
  • RNA: x 3 types
• Ligand: x 1 types

• Keywords: 48S / initiation / eIF3 / ternary complex / translation / open state / RIBOSOME

Function / homology

Function:

translation initiation ternary complex / regulation of translation in response to endoplasmic reticulum stress / glial limiting end-foot / HRI-mediated signaling / response to manganese-induced endoplasmic reticulum stress / viral translational termination-reinitiation / Cellular response to mitochondrial stress / positive regulation of type B pancreatic cell apoptotic process / Response of EIF2AK1 (HRI) to heme deficiency / Recycling of eIF2:GDP / methionyl-initiator methionine tRNA binding / negative regulation of translational initiation in response to stress / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / PERK-mediated unfolded protein response / eukaryotic translation initiation factor 3 complex, eIF3m / PERK regulates gene expression / response to kainic acid / IRES-dependent viral translational initiation / translation reinitiation / eukaryotic translation initiation factor 2 complex / formation of cytoplasmic translation initiation complex / multi-eIF complex / regulation of translational initiation in response to stress / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding / mRNA cap binding / formation of translation preinitiation complex / eukaryotic 48S preinitiation complex / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / positive regulation of ubiquitin-protein transferase activity / regulation of translational initiation / positive regulation of respiratory burst involved in inflammatory response / positive regulation of gastrulation / protein-synthesizing GTPase / protein tyrosine kinase inhibitor activity / positive regulation of DNA-templated transcription initiation / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / IRE1-RACK1-PP2A complex / positive regulation of Golgi to plasma membrane protein transport / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / nucleolus organization / TNFR1-mediated ceramide production / negative regulation of RNA splicing / neural crest cell differentiation / metal-dependent deubiquitinase activity / supercoiled DNA binding / cytoplasmic translational initiation / NF-kappaB complex / negative regulation of DNA repair / oxidized purine DNA binding / cysteine-type endopeptidase activator activity involved in apoptotic process / rRNA modification in the nucleus and cytosol / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / negative regulation of bicellular tight junction assembly / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / negative regulation of phagocytosis / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / Formation of the ternary complex, and subsequently, the 43S complex / ion channel inhibitor activity / protein kinase A binding / laminin receptor activity / Ribosomal scanning and start codon recognition / pigmentation / positive regulation of mitochondrial depolarization / Translation initiation complex formation / negative regulation of Wnt signaling pathway / fibroblast growth factor binding / Protein hydroxylation / monocyte chemotaxis / BH3 domain binding / negative regulation of translational frameshifting / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / SARS-CoV-1 modulates host translation machinery / positive regulation of GTPase activity / mTORC1-mediated signalling / TOR signaling / iron-sulfur cluster binding / Peptide chain elongation / regulation of cell division / cellular response to ethanol / Selenocysteine synthesis / Formation of a pool of free 40S subunits / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of protein binding / Eukaryotic Translation Termination / protein serine/threonine kinase inhibitor activity / SRP-dependent cotranslational protein targeting to membrane / Response of EIF2AK4 (GCN2) to amino acid deficiency / ubiquitin ligase inhibitor activity / negative regulation of respiratory burst involved in inflammatory response / Viral mRNA Translation / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)

Domain/homology:

Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor, beta propellor-like domain / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / : / Eukaryotic translation initiation factor eIF2A / eIF3 subunit 6 N terminal domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / EIF3CL-like, C-terminal domain / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / : / eIF3a, PCI domain, TPR-like region / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / : / Eukaryotic translation initiation factor 2 alpha subunit / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / S1 domain profile. / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S2, eukaryotic / Elongation factor Tu domain 2 / 40S Ribosomal protein S10 / S27a-like superfamily / Plectin/S10, N-terminal / Plectin/S10 domain / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25

Component:

Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 2 subunit 1 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Eukaryotic translation initiation factor 2 subunit 3 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 1A, X-chromosomal / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Yi S-H / Petrychenko V / Schliep JE / Goyal A / Linden A / Chari A / Urlaub H / Stark H / Rodnina MV / Adio S / Fischer N

Funding support

Germany, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	Leibniz Prize (to M.V.R.) & SFB 860	Germany
Max Planck Society		Germany

• Citation: Journal: Nucleic Acids Res / Year: 2022; Title: Conformational rearrangements upon start codon recognition in human 48S translation initiation complex.; Authors: Sung-Hui Yi / Valentyn Petrychenko / Jan Erik Schliep / Akanksha Goyal / Andreas Linden / Ashwin Chari / Henning Urlaub / Holger Stark / Marina V Rodnina / Sarah Adio / Niels Fischer / ; Abstract: Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct ribosome populations formed on an mRNA with a cognate AUG codon in the presence of eIF1, eIF1A, eIF2-GTP-Met-tRNAiMet and eIF3. The 'open' 40S subunit conformation differs from the human 48S scanning complex and represents an intermediate preceding the codon recognition step. The 'closed' form is similar to reported structures of complexes from yeast and mammals formed upon codon recognition, except for the orientation of eIF1A, which is unique in our structure. Kinetic experiments show how various initiation factors mediate the population distribution of open and closed conformations until 60S subunit docking. Our results provide insights into the timing and structure of human translation initiation intermediates and suggest the differences in the mechanisms of start codon selection between mammals and yeast.

History

Deposition	Jan 3, 2022	-
Header (metadata) release	May 11, 2022	-
Map release	May 11, 2022	-
Update	Apr 24, 2024	-
Current status	Apr 24, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_14114.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.16 Å

Density

Contour Level	By AUTHOR: 2.15
Minimum - Maximum	-11.100790999999999 - 24.270873999999999
Average (Standard dev.)	0.000000000006434 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 417.59998 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_14114_msk_1.map

Half map: #2

• File: emd_14114_half_map_1.map

Half map: #1

• File: emd_14114_half_map_2.map

Sample components

Entire : Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA

• Entire: Name: Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA

Components

• Complex: Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit B
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit K
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit F
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit L
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit M
  • RNA: mRNA
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit H
  • Protein or peptide: 60S ribosomal protein L41
  • RNA: 18S rRNA
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S30
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit G
  • Protein or peptide: Eukaryotic translation initiation factor 1A, X-chromosomal
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 1
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 3
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
  • RNA: Initiator Met-tRNA-i
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit D
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit C
• Ligand: ZINC ION

Supramolecule #1: Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA

• Supramolecule: Name: Human 48S initiation complex 40S-eIF1-eIF1A-eIF2-eIF3-tRNA-Met-mRNA; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51

Macromolecule #1: Eukaryotic translation initiation factor 3 subunit B

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.464297 KDa

Sequence

String:

MQDAENVAVP EAAEERAEPG QQQPAAEPPP AEGLLRPAGP GAPEAAGTEA SSEEVGIAEA GPESEVRTEP AAEAEAASGP SESPSPPAA EELPGSHAEP PVPAQGEAPG EQARDERSDS RAQAVSEDAG GNEGRAAEAE PRALENGDAD EPSFSDPEDF V DDVSEEEL LGDVLKDRPQ EADGIDSVIV VDNVPQVGPD RLEKLKNVIH KIFSKFGKIT NDFYPEEDGK TKGYIFLEYA SP AHAVDAV KNADGYKLDK QHTFRVNLFT DFDKYMTISD EWDIPEKQPF KDLGNLRYWL EEAECRDQYS VIFESGDRTS IFW NDVKDP VSIEERARWT ETYVRWSPKG TYLATFHQRG IALWGGEKFK QIQRFSHQGV QLIDFSPCER YLVTFSPLMD TQDD PQAII IWDILTGHKK RGFHCESSAH WPIFKWSHDG KFFARMTLDT LSIYETPSMG LLDKKSLKIS GIKDFSWSPG GNIIA FWVP EDKDIPARVT LMQLPTRQEI RVRNLFNVVD CKLHWQKNGD YLCVKVDRTP KGTQGVVTNF EIFRMREKQV PVDVVE MKE TIIAFAWEPN GSKFAVLHGE APRISVSFYH VKNNGKIELI KMFDKQQANT IFWSPQGQFV VLAGLRSMNG ALAFVDT SD CTVMNIAEHY MASDVEWDPT GRYVVTSVSW WSHKVDNAYW LWTFQGRLLQ KNNKDRFCQL LWRPRPPTLL SQEQIKQI K KDLKKYSKIF EQKDRLSQSK ASKELVERRR TMMEDFRKYR KMAQELYMEQ KNERLELRGG VDTDELDSNV DDWEEETIE FFVTEEIIPL GNQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit B

Macromolecule #2: Eukaryotic translation initiation factor 3 subunit K

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.083619 KDa

Sequence

String:

MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLSDS QLKVWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit K

Macromolecule #3: Eukaryotic translation initiation factor 3 subunit F

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: ubiquitinyl hydrolase 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.593645 KDa

Sequence

String:

MATPAVPVSA PPATPTPVPA AAPASVPAPT PAPAAAPVPA AAPASSSDPA AAAAATAAPG QTPASAQAPA QTPAPALPGP ALPGPFPGG RVVRLHPVIL ASIVDSYERR NEGAARVIGT LLGTVDKHSV EVTNCFSVPH NESEDEVAVD MEFAKNMYEL H KKVSPNEL ILGWYATGHD ITEHSVLIHE YYSREAPNPI HLTVDTSLQN GRMSIKAYVS TLMGVPGRTM GVMFTPLTVK YA YYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVP DDFETM LNSNINDLLM VTYLANLTQS QIALNEKLVN L

UniProtKB: Eukaryotic translation initiation factor 3 subunit F

Macromolecule #4: Eukaryotic translation initiation factor 3 subunit L

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 66.803734 KDa

Sequence

String:

MSYPADDYES EAAYDPYAYP SDYDMHTGDP KQDLAYERQY EQQTYQVIPE VIKNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVY EIQDIYENSW TKLTERFFKN TPWPEAEAIA PQVGNDAVFL ILYKELYYRH IYAKVSGGPS LEQRFESYYN Y CNLFNYIL NADGPAPLEL PNQWLWDIID EFIYQFQSFS QYRCKTAKKS EEEIDFLRSN PKIWNVHSVL NVLHSLVDKS NI NRQLEVY TSGGDPESVA GEYGRHSLYK MLGYFSLVGL LRLHSLLGDY YQAIKVLENI ELNKKSMYSR VPECQVTTYY YVG FAYLMM RRYQDAIRVF ANILLYIQRT KSMFQRTTYK YEMINKQNEQ MHALLAIALT MYPMRIDESI HLQLREKYGD KMLR MQKGD PQVYEELFSY SCPKFLSPVV PNYDNVHPNY HKEPFLQQLK VFSDEVQQQA QLSTIRSFLK LYTTMPVAKL AGFLD LTEQ EFRIQLLVFK HKMKNLVWTS GISALDGEFQ SASEVDFYID KDMIHIADTK VARRYGDFFI RQIHKFEELN RTLKKM GQR P

UniProtKB: Eukaryotic translation initiation factor 3 subunit L

Macromolecule #5: Eukaryotic translation initiation factor 3 subunit M

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 42.555832 KDa

Sequence

String:

MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

UniProtKB: Eukaryotic translation initiation factor 3 subunit M

Macromolecule #7: Eukaryotic translation initiation factor 3 subunit H

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.979277 KDa

Sequence

String:

MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR LTPKLMEVCK EKDFSPEALK KANITFEYMF EEVPIVIKNS HLINVLMWEL EKKSAVADKH ELLSLASSNH LG KNLQLLM DRVDEMSQDI VKYNTYMRNT SKQQQQKHQY QQRRQQENMQ RQSRGEPPLP EEDLSKLFKP PQPPARMDSL LIA GQINTY CQNIKEFTAQ NLGKLFMAQA LQEYNN

UniProtKB: Eukaryotic translation initiation factor 3 subunit H

Macromolecule #8: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #10: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #11: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #12: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #13: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #14: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.668938 KDa

Sequence

String:

KVHGSLARAG KVRGQTPKVA KQEKKKKKTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #15: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #16: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #17: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #18: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #19: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #20: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.376516 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG NRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW MPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #21: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.578156 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGPV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #22: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.883938 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTDWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #23: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #24: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #25: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #26: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #27: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #28: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #29: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #30: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #31: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.729369 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPTGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #32: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #33: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #34: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #35: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.091562 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #36: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #37: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #38: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #39: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #40: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #41: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #42: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #43: Eukaryotic translation initiation factor 3 subunit G

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit G / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.662016 KDa

Sequence

String:

MPTGDFDSKP SWADQVEEEG EDDKCVTSEL LKGIPLATGD TSPEPELLPG APLPPPKEVI NGNIKTVTEY KIDEDGKKFK IVRTFRIET RKASKAVARR KNWKKFGNSE FDPPGPNVAT TTVSDDVSMT FITSKEDLNC QEEEDPMNKL KGQKIVSCRI C KGDHWTTR CPYKDTLGPM QKELAEQLGL STGEKEKLPG ELEPVQATQN KTGKYVPPSL RDGASRRGES MQPNRRADDN AT IRVTNLS EDTRETDLQE LFRPFGSISR IYLAKDKTTG QSKGFAFISF HRREDAARAI AGVSGFGYDH LILNVEWAKP STN

UniProtKB: Eukaryotic translation initiation factor 3 subunit G

Macromolecule #44: Eukaryotic translation initiation factor 1A, X-chromosomal

• Macromolecule: Name: Eukaryotic translation initiation factor 1A, X-chromosomal; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.488449 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKA DVILKYNADE ARSLKAYGEL PEHAKINETD TFGPGDDDEI QFDDIGDDDE DIDDI

UniProtKB: Eukaryotic translation initiation factor 1A, X-chromosomal

Macromolecule #45: Eukaryotic translation initiation factor 2 subunit 1

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.16118 KDa

Sequence

String:

MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYGYEGIDAV KEALRAGLNC STENMPIKIN LIAPPRYVMT TT TLERTEG LSVLSQAMAV IKEKIEEKRG VFNVQMEPKV VTDTDETELA RQMERLEREN AEVDGDDDAE EMEAKAED

UniProtKB: Eukaryotic translation initiation factor 2 subunit 1

Macromolecule #46: Eukaryotic translation initiation factor 2 subunit 3

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 3 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 51.178406 KDa

Sequence

String:

MAGGEAGVTL GQPHLSRQDL TTLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIY KLDDPSCPRP ECYRSCGSST PDEFPTDIPG TKGNFKLVRH VSFVDCPGHD ILMATMLNGA AVMDAALLLI A GNESCPQP QTSEHLAAIE IMKLKHILIL QNKIDLVKES QAKEQYEQIL AFVQGTVAEG APIIPISAQL KYNIEVVCEY IV KKIPVPP RDFTSEPRLI VIRSFDVNKP GCEVDDLKGG VAGGSILKGV LKVGQEIEVR PGIVSKDSEG KLMCKPIFSK IVS LFAEHN DLQYAAPGGL IGVGTKIDPT LCRADRMVGQ VLGAVGALPE IFTELEISYF LLRRLLGVRT EGDKKAAKVQ KLSK NEVLM VNIGSLSTGG RVSAVKADLG KIVLTNPVCT EVGEKIALSR RVEKHWRLIG WGQIRRGVTI KPTVDDD

UniProtKB: Eukaryotic translation initiation factor 2 subunit 3

Macromolecule #47: Eukaryotic translation initiation factor 3 subunit A

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 166.903781 KDa

Sequence

String:

MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKMAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQNNT ILRLLQQVSQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGD SSLSRKDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDEDRS HRRDEERPRR LGDDEDREPS LRPDDDRVPR R GMDDDRGP RRGPEEDRFS RRGADDDRPS WRNTDDDRPP RRIADEDRGN WRHADDDRPP RRGLDEDRGS WRTADEDRGP RR GMDDDRG PRRGGADDER SSWRNADDDR GPRRGLDDDR GPRRGMDDDR GPRRGMDDDR GPRRGMDDDR GPRRGLDDDR GPW RNADDD RIPRRGAEDD RGPWRNMDDD RLSRRADDDR FPRRGDDSRP GPWRPLVKPG GWREKEKARE ESWGPPRESR PSEE REWDR EKERDRDNQD REENDKDPER ERDRERDVDR EDRFRRPRDE GGWRRGPAEE SSSWRDSSRR DDRDRDDRRR ERDDR RDLR ERRDLRDDRD RRGPPLRSER EEVSSWRRAD DRKDDRVEER DPPRRVPPPA LSRDRERDRD REREGEKEKA SWRAEK DRE SLRRTKNETD EDGWTTVRR

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

Macromolecule #48: Eukaryotic translation initiation factor 3 subunit E

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.281633 KDa

Sequence

String:

MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETE PIVKMFEDPE TTRQMQSTRD GRMLFDYLAD KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP A TDRNALSS LWGKLASEIL MQNWDAAMED LTRLKETIDN NSVSSPLQSL QQRTWLIHWS LFVFFNHPKG RDNIIDLFLY QP QYLNAIQ TMCPHILRYL TTAVITNKDV RKRRQVLKDL VKVIQQESYT YKDPITEFVE CLYVNFDFDG AQKKLRECES VLV NDFFLV ACLEDFIENA RLFIFETFCR IHQCISINML ADKLNMTPEE AERWIVNLIR NARLDAKIDS KLGHVVMGNN AVSP YQQVI EKTKSLSFRS QMLAMNIEKK LNQNSRSEAP NWATQDSGFY

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

Macromolecule #50: Eukaryotic translation initiation factor 3 subunit D

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 64.060758 KDa

Sequence

String:

MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY QDKRYTNKYS SQFGGGSQYA YFHEEDESSF QLVDTARTQ KTAYQRNRMR FAQRNLRRDK DRRNMLQFNL QILPKSAKQK ERERIRLQKK FQKQFGVRQK WDQKSQKPRD S SVEVRSDW EVKEEMDFPQ LMKMRYLEVS EPQDIECCGA LEYYDKAFDR ITTRSEKPLR SIKRIFHTVT TTDDPVIRKL AK TQGNVFA TDAILATLMS CTRSVYSWDI VVQRVGSKLF FDKRDNSDFD LLTVSETANE PPQDEGNSFN SPRNLAMEAT YIN HNFSQQ CLRMGKERYN FPNPNPFVED DMDKNEIASV AYRYRRWKLG DDIDLIVRCE HDGVMTGANG EVSFINIKTL NEWD SRHCN GVDWRQKLDS QRGAVIATEL KNNSYKLARW TCCALLAGSE YLKLGYVSRY HVKDSSRHVI LGTQQFKPNE FASQI NLSV ENAWGILRCV IDICMKLEEG KYLILKDPNK QVIRVYSLPD GTFSSDEDEE EEEEEEEEEE EEET

UniProtKB: Eukaryotic translation initiation factor 3 subunit D

Macromolecule #51: Eukaryotic translation initiation factor 3 subunit C

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 105.503945 KDa

Sequence

String:

MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITSY K QNPEQSAD EDAEKNEEDS EGSSDEDEDE DGVSAATFLK KKSEAPSGES RKFLKKMDDE DEDSEDSEDD EDWDTGSTSS DS DSEEEEG KQTALASRFL KKAPTTDEDK KAAEKKREDK AKKKHDRKSK RLDEEEEDNE GGEWERVRGG VPLVKEKPKM FAK GTEITH AVVIKKLNEI LQARGKKGTD RAAQIELLQL LVQIAAENNL GEGVIVKIKF NIIASLYDYN PNLATYMKPE MWGK CLDCI NELMDILFAN PNIFVGENIL EESENLHNAD QPLRVRGCIL TLVERMDEEF TKIMQNTDPH SQEYVEHLKD EAQVC AIIE RVQRYLEEKG TTEEVCRIYL LRILHTYYKF DYKAHQRQLT PPEGSSKSEQ DQAENEGEDS AVLMERLCKY IYAKDR TDR IRTCAILCHI YHHALHSRWY QARDLMLMSH LQDNIQHADP PVQILYNRTM VQLGICAFRQ GLTKDAHNAL LDIQSSG RA KELLGQGLLL RSLQERNQEQ EKVERRRQVP FHLHINLELL ECVYLVSAML LEIPYMAAHE SDARRRMISK QFHHQLRV G ERQPLLGPPE SMREHVVAAS KAMKMGDWKT CHSFIINEKM NGKVWDLFPE ADKVRTMLVR KIQEESLRTY LFTYSSVYD SISMETLSDM FELDLPTVHS IISKMIINEE LMASLDQPTQ TVVMHRTEPT AQQNLALQLA EKLGSLVENN ERVFDHKQGT YGGYFRDQK DGYRKNEGYM RRGGYRQQQS QTAY

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

Macromolecule #6: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 82.412445 KDa

Sequence

String:

GGGCAACAAC AACAAGCUAG CCACAACAAC AACAACAACA ACAACAAGUC GACCAACAAC AACAACAACA ACAACAACAA CUCGAGCAA CAACAACAAC AACAACAACA ACAAGGAUCC AAAACAGACC ACCAUGGUAC GUUUCAAGGC UUGAGCCCUC G UCACUGCC CUGUGGGGCA AGGUGACUCU GGAAGAAGUU GGUGGUGAGG CCCUGGGCAG GCUGCAGAGU GUGAGGGAAG GU CUGGUUG UCUACCAA

Macromolecule #9: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 603.102312 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUG AGUACGCACG GCCGGU ACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGU(OMU)CC(PSU) U(OMU)GGUCGCUC GCUCCUCUCC UACUU GGAU AACUGUGGUA (A2M)UUCUAG(A2M)GC UAAUA(OMC)AUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGC GUGCA UUUAUCAGAU CAAAACCAAC CCGGUCAGCC CCUCUCCGGC CCCGGCCGGG GGGCGGGCGC CGGCGGCUUU GGUGA CUCU AGAUAACCUC GGGCCGAUCG CACGCCCCCC GUGGCGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAU GGU AGUCGCCGUG CCUACCAUGG UGACCACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGG CU ACCACAUCCA AGGAAGGCAG CAGGCGCGC(A2M) AAUUACCCAC UCCCGACCCG GGGA(OMG)GUAGU GA(OMC)GAA AAA UAACAAUACA GGACUCUUUC GAGGCCCUGU AAUUGGAAUG AGUCCACUUU AAAUCCUUUA ACGAGGAUCC AUUGGAG GG CAAGUCUGG(PSU) GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU A(OMG)CGUAUAUU AAAGUUGCUG CAGUU(A2M) AAA AGCUCGUAGU U(OMG)GAUCUUGG GAGCGGGCGG GCGGUCCGCC GCGAGGCGAG CCACCGCCCG UCCCCGCCCC UUG CCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCGUU UACUUUGAAA AAA(5MU)UAGAGU G(PSU)(PSU)CAAAGC AGGCCCGAGC CGCCUGGAUA CCGCAGCUAG GAAUAAUGGA AUAGGACCGC GGUUCUAUUU UGU UGGUUU UCGGAACUGA GGCCAUGAUU AAGAGGGACG GCCGGGGGCA UUCGUAUUGC GCCGCUAGAG GUGAAAUUCU UGGA CCGGC GCAAGACGGA CCAGAGCGAA AGCAUUUGCC AAGAAUGUUU UCAUUAAUCA AGA(A2M)CGAAAG UCGGAGGUUC G AAGACGAU CAGAUACCGU CGUAGUUCCG ACCA(PSU)AAACG AUGCCGACCG GCGAUGCGGC GGCGUUAUUC CCAUGACC C GCCGGGCAGC UUCCGGGAAA CCAAAGUCUU UGGGUUCCGG GGGGAGUAUG GUUGCAAAGC UGAAACUUAA AGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAU(PSU)U GACUCAACAC GGGAAACCUC ACCCGGCCCG GACACG GAC AGGAUUGACA GAUUGAUAGC UCUUUCUCGA UUCCGUGGGU GGUGGUGCAU GGCCGUUCUU AGUUGGUGGA GCGAUUU GU CUGGUUAAUU C(5MC)GAUAACGA ACGAGACUCU GGCAUGCUAA CUAGUUACGC GACCCCCGAG CGGUCGGCGU CCCC CAACU UCUUAGAGGG ACAAGUGGCG UUCAGCCACC CGAGAUUGAG CAAUAACAGG UCUGUGAUGC CCUUAGAUGU CCGGG GCUG CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU ACGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUU CGU GAUGGGGAUC GGGGAUUGCA AUUAUUCCCC AUGAACGAGG AAUUCCCAGU AAGUGCGGGU CAUAAGCUUG CGUUGAU U(A2M) AGUCCCUGCC CUUUGUACAC ACCG(OMC)CCGUC GCUACUACCG AUUGGAUGGU UUAGUGAGGC CCUCGGAUCG GCCCCGCCG GGGUCGGCCC ACGGCCCUGG CGGAGCGCUG AGAAGACGGU CGAACUUGAC UAUCUAGAGG AAGUAAAAGU C G(UR3)AACAAG GUUUCCGUAG GUG(MA6)(MA6)CCUGC GGAAGGAUCA UUA

Macromolecule #49: Initiator Met-tRNA-i

• Macromolecule: Name: Initiator Met-tRNA-i / type: rna / ID: 49 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.23151 KDa

Sequence

String:

AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

GENBANK: GENBANK: K00328.1

Macromolecule #52: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 52 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5 ; Details: 20 mM Hepes, pH 7.5, 95 mM KOAc, 3.75 mM Mg(OAc)2, 1 mM ATP, 0.5 mM GTP, 0.25 mM spermidine, 2 mM DTT, 0.4 U/uL RiboLock RNase inhibitor
• Grid: Model: Quantifoil R3.5/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV; Details: Cryo-EM grids were prepared by floating home-made continuous carbon on 40 ul sample in the wells of teflon block (custom-made). The sample-covered carbon was then adsorbed to an EM grid..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Details: Aberration corrections performed using Cs image corrector (CEOS company)
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 15544 / Average exposure time: 1.0 sec. / Average electron dose: 48.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.01 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 990486

Startup model

Type of model: EMDB MAP
EMDB ID:

2450

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 364950
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final 3D classification: Software - Name: RELION (ver. 3.1)

Atomic model buiding 1

Initial model

PDB ID:

6zmw

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL / Protocol: FLEXIBLE FIT / Target criteria: RSCC

Output model

PDB-7qp7:
Structure of the human 48S initiation complex in closed state (h48S AUG closed)