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TitleConformational rearrangements upon start codon recognition in human 48S translation initiation complex.
Journal, issue, pagesNucleic Acids Res, Vol. 50, Issue 9, Page 5282-5298, Year 2022
Publish dateMay 20, 2022
AuthorsSung-Hui Yi / Valentyn Petrychenko / Jan Erik Schliep / Akanksha Goyal / Andreas Linden / Ashwin Chari / Henning Urlaub / Holger Stark / Marina V Rodnina / Sarah Adio / Niels Fischer /
PubMed AbstractSelection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of ...Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct ribosome populations formed on an mRNA with a cognate AUG codon in the presence of eIF1, eIF1A, eIF2-GTP-Met-tRNAiMet and eIF3. The 'open' 40S subunit conformation differs from the human 48S scanning complex and represents an intermediate preceding the codon recognition step. The 'closed' form is similar to reported structures of complexes from yeast and mammals formed upon codon recognition, except for the orientation of eIF1A, which is unique in our structure. Kinetic experiments show how various initiation factors mediate the population distribution of open and closed conformations until 60S subunit docking. Our results provide insights into the timing and structure of human translation initiation intermediates and suggest the differences in the mechanisms of start codon selection between mammals and yeast.
External linksNucleic Acids Res / PubMed:35489072 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 4.7 Å
Structure data

14113

7qp6

EMDB-14113, PDB-7qp6:
Structure of the human 48S initiation complex in open state (h48S AUG open)
Method: EM (single particle) / Resolution: 4.7 Å

14114

7qp7

EMDB-14114, PDB-7qp7:
Structure of the human 48S initiation complex in closed state (h48S AUG closed)
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRIBOSOME / 48S / initiation / eIF3 / ternary complex / translation / open state

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