Biotechnology and Biological Sciences Research Council (BBSRC)
BB/P01948X/1
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/R002517/1
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/S003339/1
United Kingdom
Medical Research Council (MRC, United Kingdom)
MR/S009213/1
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/R018375/1
United Kingdom
Wellcome Trust
202642/Z/16/Z
United Kingdom
Citation
Journal: Nat Commun / Year: 2021 Title: Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Authors: Yoel A Klug / Justin C Deme / Robin A Corey / Mike F Renne / Phillip J Stansfeld / Susan M Lea / Pedro Carvalho / Abstract: Lipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by a phospholipid monolayer. This unique architecture is generated ...Lipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by a phospholipid monolayer. This unique architecture is generated during LD biogenesis at endoplasmic reticulum (ER) sites marked by Seipin, a conserved membrane protein mutated in lipodystrophy. Here structural, biochemical and molecular dynamics simulation approaches reveal the mechanism of LD formation by the yeast Seipin Sei1 and its membrane partner Ldb16. We show that Sei1 luminal domain assembles a homooligomeric ring, which, in contrast to other Seipins, is unable to concentrate triacylglycerol. Instead, Sei1 positions Ldb16, which concentrates triacylglycerol within the Sei1 ring through critical hydroxyl residues. Triacylglycerol recruitment to the complex is further promoted by Sei1 transmembrane segments, which also control Ldb16 stability. Thus, we propose that LD assembly by the Sei1/Ldb16 complex, and likely other Seipins, requires sequential triacylglycerol-concentrating steps via distinct elements in the ER membrane and lumen.
History
Deposition
Jun 22, 2021
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Header (metadata) release
Oct 13, 2021
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Map release
Oct 13, 2021
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Update
Oct 20, 2021
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Current status
Oct 20, 2021
Processing site: PDBe / Status: Released
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Structure visualization
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