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- EMDB-12975: Structure of Primase-Helicase in SaPI5 -

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Basic information

Entry
Database: EMDB / ID: EMD-12975
TitleStructure of Primase-Helicase in SaPI5
Map data
Sample
  • Organelle or cellular component: Primase-Helicase in SaPI5
    • Protein or peptide: DNA primase
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION
KeywordsHelicase / DNA binding / AMPPNP / REPLICATION
Function / homology
Function and homology information


DNA primase, phage/plasmid / D5 N terminal like / : / NrS-1 polymerase HBD domain / : / Domain of unknown function DUF5906 / Family of unknown function (DUF5906) / DNA primase/nucleoside triphosphatase, C-terminal / Poxvirus D5 protein-like / Bacteriophage/plasmid primase, P4, C-terminal ...DNA primase, phage/plasmid / D5 N terminal like / : / NrS-1 polymerase HBD domain / : / Domain of unknown function DUF5906 / Family of unknown function (DUF5906) / DNA primase/nucleoside triphosphatase, C-terminal / Poxvirus D5 protein-like / Bacteriophage/plasmid primase, P4, C-terminal / D5 N terminal like / Helicase, superfamily 3, DNA virus / Superfamily 3 helicase of DNA viruses domain profile. / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsQiao CC / Mir-Sanchis I
Funding support Sweden, 1 items
OrganizationGrant numberCountry
Knut and Alice Wallenberg Foundation Sweden
CitationJournal: Nucleic Acids Res / Year: 2022
Title: Staphylococcal self-loading helicases couple the staircase mechanism with inter domain high flexibility.
Authors: Cuncun Qiao / Gianluca Debiasi-Anders / Ignacio Mir-Sanchis /
Abstract: Replication is a crucial cellular process. Replicative helicases unwind DNA providing the template strand to the polymerase and promoting replication fork progression. Helicases are multi-domain ...Replication is a crucial cellular process. Replicative helicases unwind DNA providing the template strand to the polymerase and promoting replication fork progression. Helicases are multi-domain proteins which use an ATPase domain to couple ATP hydrolysis with translocation, however the role that the other domains might have during translocation remains elusive. Here, we studied the unexplored self-loading helicases called Reps, present in Staphylococcus aureus pathogenicity islands (SaPIs). Our cryoEM structures of the PriRep5 from SaPI5 (3.3 Å), the Rep1 from SaPI1 (3.9 Å) and Rep1-DNA complex (3.1Å) showed that in both Reps, the C-terminal domain (CTD) undergoes two distinct movements respect the ATPase domain. We experimentally demonstrate both in vitro and in vivo that SaPI-encoded Reps need key amino acids involved in the staircase mechanism of translocation. Additionally, we demonstrate that the CTD's presence is necessary for the maintenance of full ATPase and helicase activities. We speculate that this high interdomain flexibility couples Rep's activities as initiators and as helicases.
History
DepositionMay 19, 2021-
Header (metadata) releaseJul 13, 2022-
Map releaseJul 13, 2022-
UpdateNov 13, 2024-
Current statusNov 13, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_12975.png

Downloads & links

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Map

FileDownload / File: emd_12975.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 360 pix.
= 295.2 Å		0.82 Å/pix.
x 360 pix.
= 295.2 Å		0.82 Å/pix.
x 360 pix.
= 295.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.82 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.008
Minimum - Maximum-0.012518998 - 0.039599884
Average (Standard dev.)0.000029685309 (±0.0013586174)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 295.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_12975_msk_1.map
Projections & Slices
AxesZYX

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Histogram

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Half map: #2

Fileemd_12975_half_map_1.map
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Half map: #1

Fileemd_12975_half_map_2.map
Projections & Slices
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Sample components

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Entire : Primase-Helicase in SaPI5

EntireName: Primase-Helicase in SaPI5
Components
  • Organelle or cellular component: Primase-Helicase in SaPI5
    • Protein or peptide: DNA primase
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Primase-Helicase in SaPI5

SupramoleculeName: Primase-Helicase in SaPI5 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 669 KDa

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Macromolecule #1: DNA primase

MacromoleculeName: DNA primase / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 93.007031 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MGHHHHHHAI KKKDRIIGVK ELEIPQELKL VPNWVLWRAE WNEKQQNFGK VPYSINGYRA STTNKKTWCD FESVSIEYEV DEQYSGIGF VLSDGNNFVC LDIDNAIDKK GQINSELALK MMQLTYCEKS PSGTGLHCFF KGKLPDNRKK KRTDLDIELY D SARFMTVT ...String:
MGHHHHHHAI KKKDRIIGVK ELEIPQELKL VPNWVLWRAE WNEKQQNFGK VPYSINGYRA STTNKKTWCD FESVSIEYEV DEQYSGIGF VLSDGNNFVC LDIDNAIDKK GQINSELALK MMQLTYCEKS PSGTGLHCFF KGKLPDNRKK KRTDLDIELY D SARFMTVT GCTIGQSDIC DNQEVLNTLV DEYFKENLPA NEVVREESNT NIQLSDEDII NIMMKSKQKD KIKDLLQGTY ES YFESSSE AVQSLLHYLA FYTGKNKQQM ERIFLNYNNL TDKWESKRGN TTWGQLELDK AIKNQKTIYT KSIDEFNVIP QGS KDVKQL LNQLGHEERT KMEENWIEEG KRGRKPTTIS PIKCAYILNE HLTFILFDDE ENTKLAMYQF DEGIYTQNTT IIKR VISYL EPKHNSNKAD EVIYHLTNMV DIKEKTNSPY LIPVKNGVFN RKTKQLESFT PDYIFTSKID TSYVRQDIVP EINGW NIDR WIEEIACNDN QVVKLLWQVI NDSMNGNYTR KKAIFFVGDG NNGKGTFQEL LSNVIGYSNI ASLKVNEFDE RFKLSV LEG KTAVIGDDVP VGVYVDDSSN FKSVVTGDPV LVEFKNKPLY RATFKCTVIQ STNGMPKFKD KTGGTLRRLL IVPFNAN FN GIKENFKIKE DYIKNQQVLE YVLYKAINLD FETFDIPDAS KKMLEVFKED NDPVYGFKVN MFDQWTIRKV PKYIVYAF Y KEYCDENGYN ALSSNKFYKQ FEHYLENYWK TDAQRRYDNE ELAKRIYNFN DNRNYIEPIE SGKNYKSYEK VKLKAI

UniProtKB: DNA primase

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Macromolecule #2: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 2 / Number of copies: 4 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Macromolecule #3: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 3 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.4 mg/mL
BufferpH: 8 / Component - Concentration: 200.0 mM / Component - Formula: NaCl / Component - Name: Sodium Chloride
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Number real images: 4760 / Average exposure time: 4.0 sec. / Average electron dose: 1.16 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 165000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 724786
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 185358
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL / Target criteria: Correlation coefficient
Output model

PDB-7ola:
Structure of Primase-Helicase in SaPI5

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