H2020 Marie Curie Actions of the European Commission
894862
ドイツ
German Research Foundation (DFG)
EXC 2067/1 39072994
ドイツ
German Research Foundation (DFG)
SFB860
ドイツ
German Research Foundation (DFG)
SPP2191
ドイツ
European Research Council (ERC)
882357
ドイツ
引用
ジャーナル: Cell / 年: 2021 タイトル: Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening. 著者: Sandra Schilbach / Shintaro Aibara / Christian Dienemann / Frauke Grabbe / Patrick Cramer / 要旨: Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of ...Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30-35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase "clamp head loop" and the TFIIF "charged region" that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems.