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- EMDB-12672: Dimeric Photosystem I of a temperature sensitive mutant Chlamydom... -

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Basic information

Entry
Database: EMDB / ID: EMD-12672
TitleDimeric Photosystem I of a temperature sensitive mutant Chlamydomonas reinhardtii
Map data
Sample
  • Complex: Photosystem I of temperature sensitive Chlamydomonas reinhardtii
    • Protein or peptide: x 20 types
Function / homology
Function and homology information


chloroplast thylakoid lumen / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chloroplast thylakoid membrane / chlorophyll binding ...chloroplast thylakoid lumen / photosynthesis, light harvesting / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chloroplast thylakoid membrane / chlorophyll binding / response to light stimulus / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily ...Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Chlorophyll A-B binding protein, plant and chromista / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Photosystem I PsaD / Photosystem I, reaction centre subunit PsaD superfamily / PsaD / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I reaction centre subunit IX / PsaJ / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / : / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I reaction center subunit VIII / Chlorophyll a-b binding protein, chloroplastic / PSI subunit V / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit IX ...Photosystem I reaction center subunit VIII / Chlorophyll a-b binding protein, chloroplastic / PSI subunit V / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center / Photosystem I reaction center subunit II, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant) / Chlamydomonas smithii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 17.1 Å
AuthorsCaspy I / Nelson N
Funding support Israel, 2 items
OrganizationGrant numberCountry
Israel Science Foundation569/17 Israel
German-Israeli Foundation for Research and DevelopmentG-1483-207/2018 Israel
CitationJournal: Commun Biol / Year: 2021
Title: Dimeric and high-resolution structures of Chlamydomonas Photosystem I from a temperature-sensitive Photosystem II mutant.
Authors: Ido Caspy / Tom Schwartz / Vinzenz Bayro-Kaiser / Mariia Fadeeva / Amit Kessel / Nir Ben-Tal / Nathan Nelson /
Abstract: Water molecules play a pivotal functional role in photosynthesis, primarily as the substrate for Photosystem II (PSII). However, their importance and contribution to Photosystem I (PSI) activity ...Water molecules play a pivotal functional role in photosynthesis, primarily as the substrate for Photosystem II (PSII). However, their importance and contribution to Photosystem I (PSI) activity remains obscure. Using a high-resolution cryogenic electron microscopy (cryo-EM) PSI structure from a Chlamydomonas reinhardtii temperature-sensitive photoautotrophic PSII mutant (TSP4), a conserved network of water molecules - dating back to cyanobacteria - was uncovered, mainly in the vicinity of the electron transport chain (ETC). The high-resolution structure illustrated that the water molecules served as a ligand in every chlorophyll that was missing a fifth magnesium coordination in the PSI core and in the light-harvesting complexes (LHC). The asymmetric distribution of the water molecules near the ETC branches modulated their electrostatic landscape, distinctly in the space between the quinones and FX. The data also disclosed the first observation of eukaryotic PSI oligomerisation through a low-resolution PSI dimer that was comprised of PSI-10LHC and PSI-8LHC.
History
DepositionMar 25, 2021-
Header (metadata) releaseDec 15, 2021-
Map releaseDec 15, 2021-
UpdateDec 15, 2021-
Current statusDec 15, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7o01
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12672.png

Downloads & links

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Map

FileDownload / File: emd_12672.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.48 Å/pix.
x 200 pix.
= 496.2 Å		2.48 Å/pix.
x 200 pix.
= 496.2 Å		2.48 Å/pix.
x 200 pix.
= 496.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.481 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.011 / Movie #1: 0.02
Minimum - Maximum-0.034410067 - 0.09448191
Average (Standard dev.)0.0011442363 (±0.008196531)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 496.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.4812.4812.481
M x/y/z200200200
origin x/y/z0.0000.0000.000
length x/y/z496.200496.200496.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS200200200
D min/max/mean-0.0340.0940.001

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Supplemental data

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Sample components

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Entire : Photosystem I of temperature sensitive Chlamydomonas reinhardtii

EntireName: Photosystem I of temperature sensitive Chlamydomonas reinhardtii
Components
  • Complex: Photosystem I of temperature sensitive Chlamydomonas reinhardtii
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit IV, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit III, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit V, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit psaK, chloroplastic
    • Protein or peptide: PSI subunit V
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic

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Supramolecule #1: Photosystem I of temperature sensitive Chlamydomonas reinhardtii

SupramoleculeName: Photosystem I of temperature sensitive Chlamydomonas reinhardtii
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 890 KDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 82.121938 KDa
SequenceString: KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLSG MYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL YTTAIGGLVM A AAMFFAGW ...String:
KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLSG MYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL YTTAIGGLVM A AAMFFAGW FHYHKAAPKL EWFQNVESML NHHLGGLLGL GSLAWAGHQI HVSLPVNKLL DAGVDPKEIP LPHDLLLNRA IM ADLYPSF AKGIAPFFTL NWSEYSDFLT FKGGLNPVTG GLWLSDTAHH HVAIAVLFLV AGHMYRTNWG IGHSMKEILE AHR GPFTGE GHVGLYEILT TSWHAQLAIN LALFGSLSII VAHHMYAMPP YPYLATDYGT QLSLFTHHTW IGGFCIVGAG AHAA IFMVR DYDPTNNYNN LLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMSAL GRPQDMFSDT AIQLQPVFAQ WIQNT HFLA PQLTAPNALA ATSLTWGGDL VAVGGKVAMM PISLGTSDFM VHHIHAFTIH VTVLILLKGV LFARSSRLIP DKANLG FRF PCDGPGRGGT CQVSAWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVT ASGVSHITGG NFAQSANTIN GWLRDFL WA QSSQVIQSYG SALSAYGLIF LGAHFVWAFS LMFLFSGRGY WQELIESIVW AHNKLKVAPA IQPRALSITQ GRAVGVAH Y LLGGIATTWS FFLARIISVG

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 81.996016 KDa
SequenceString: ATKLFPKFSQ GLAQDPTTRR IWYGLAMAHD FESHDGMTEE NLYQKIFASH FGQLSIIFLW TSGNLFHVAW QGNFEQWVTD PVHIRPIAH AIWDPHFGQP AVEAFTRGGA SGPVNISTSG VYQWWYTIGM RTNQDLYVGS VFLALVSAIF LFAGWLHLQP N FQPSLSWF ...String:
ATKLFPKFSQ GLAQDPTTRR IWYGLAMAHD FESHDGMTEE NLYQKIFASH FGQLSIIFLW TSGNLFHVAW QGNFEQWVTD PVHIRPIAH AIWDPHFGQP AVEAFTRGGA SGPVNISTSG VYQWWYTIGM RTNQDLYVGS VFLALVSAIF LFAGWLHLQP N FQPSLSWF KDAESRLNHH LSGLFGVSSL AWTGHLVHVA IPESRGQHVG WDNFLSVLPH PQGLTPFFTG NWAAYAQSPD TA SHVFGTA QGSGQAILTF LGGFHPQTQS LWLTDMAHHH LAIAVIFIVA GHMYRTNFGI GHRMQAILEA HTPPSGSLGA GHK GLFDTV NNSLHFQLGL ALASVGTITS LVAQHMYSLP PYAFQAIDFT TQAALYTHHQ YIAGFIMCGA FAHGAIFFIR DYDP EQNKG NVLARMLDHK EALISHLSWV SLFLGFHTLG LYVHNDVMQA FGTPEKQILI EPVFAQWIQA AHGKALYGFD FLLSS KTSA AFANGQSLWL PGWLDAINNN QNSLFLTIGP GDFLVHHAIA LGLHTTTLIL VKGALDARGS KLMPDKKDFG YSFPCD GPG RGGTCDISAY DAFYLAVFWM LNTIGWVTFY WHWKHLTLWQ GNVAQFDESS TYLMGWLRDY LWLNSSQLIN GYNPFGM NS LSVWAWTFLF GHLIYATGFM FLISWRGYWQ ELIETLVWAH EKTPLANLVY WKDKPVALSI VQARLVGLAH FSVGYIFT Y AAFLIASTSG RF

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 8.73813 KDa
SequenceString:
AHIVKIYDTC IGCTQCVRAC PLDVLEMVPW DGCKASQMAS APRTEDCVGC KRCETACPTD FLSVRVYLGS ESTRSMGLSY

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Macromolecule #4: Photosystem I reaction center subunit II, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit II, chloroplastic
type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 16.152684 KDa
SequenceString:
WTVPTLNPDT PSPIFGGSTG GLLRKAQTEE FYVITWEAKK EQIFEMPTGG AAIMRQGPNL LKFGKKEQCL ALTTQLRNKF KLTP(SNC)FYRV FPDGKVQYLH PADGVYPEKV NAGRVGANQN MRRIGQNVNP IKVKFSGRMM SPAEI

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Macromolecule #5: Photosystem I reaction center subunit IV, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit IV, chloroplastic
type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 7.021877 KDa
SequenceString:
EVGPKRGSLV KILRPESYWF NQVGKVVSVD QSGVRYPVVV RFENQNYAGV TTNNYALDEV VAA

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Macromolecule #6: Photosystem I reaction center subunit III, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit III, chloroplastic
type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 17.957773 KDa
SequenceString:
DIAGLTPCSE SKAYAKLEKK ELKTLEKRLK QYEADSAPAV ALKATMERTK ARFANYAKAG LLCGNDGLPH LIADPGLALK YGHAGEVFI PTFGFLYVAG YIGYVGRQYL IAVKGEAKPT DKEIIIDVPL ATKLAWQGAG WPLAAVQELQ RGTLLEKEEN I TVSPR

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Macromolecule #7: Photosystem I reaction center subunit V, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit V, chloroplastic
type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 9.572677 KDa
SequenceString:
LDPQIVISGS TAAFLAIGRF VFLGYQRREA NFDSTVGPKT TGATYFDDLQ KNSTIFATND PAGFNIIDVA GWGALGHAVG FAVLAINSL QG

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Macromolecule #8: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 3.970731 KDa
SequenceString:
PFVPPSWAPS VFVPLTGLVL PAIAMATLFV YIEKEAP

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Macromolecule #9: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 4.516257 KDa
SequenceString:
MKDFTTYLST APVIATIWFT FTAGLLIEIN RYFPDPLVF

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Macromolecule #10: Photosystem I reaction center subunit psaK, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit psaK, chloroplastic
type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 8.146467 KDa
SequenceString:
FIGSSTNLIM VASTTATLAA ARFGLAPTVK KNTTAGLKLV DSKNSAGVIS NDPAGFTIVD VLAMGAAGHG LGVGIVLGLK GIGA

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Macromolecule #11: PSI subunit V

MacromoleculeName: PSI subunit V / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 14.130248 KDa
SequenceString:
GMLETPVTSA PIVATYLSNL PAYRTGVAPV LRGVEIGLAH GFLLAGPFIK LGPLRNVPET AEIAGSLSAA GLVLILALCL SIYGSAQFQ STPSIGVKTL SGRSVARDPL FSADGWSEFA AGFLVGGEAG VAWAYVCTQ

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Macromolecule #12: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 12 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 20.368023 KDa
SequenceString: KAGNWLPGSD APAWLPDDLP GNYGFDPLSL GKEPASLKRF TESEVIHGRW AMLGVAGSLA VELLGYGNWY DAPLWAVNGG KATWFGIEV PFDLNALLAF EFVAMAAAEG QRGDAGGVVY PGGAFDPLGF AKDSSKSGEL KLKEIKNGRL AMVAFLGFVA Q HAATGKGP ...String:
KAGNWLPGSD APAWLPDDLP GNYGFDPLSL GKEPASLKRF TESEVIHGRW AMLGVAGSLA VELLGYGNWY DAPLWAVNGG KATWFGIEV PFDLNALLAF EFVAMAAAEG QRGDAGGVVY PGGAFDPLGF AKDSSKSGEL KLKEIKNGRL AMVAFLGFVA Q HAATGKGP IAALGEHLAN PWGANFATNG ISVPFF

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Macromolecule #13: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 23.689025 KDa
SequenceString: LYVGASQSSL AYLDGSLPGD FGFDPLGLLD PVNSGGFIEP KWLQYSEVIH ARWAMLGAAG CIAPEVLGAA GLIPDATNIK WFESGVIPP AGSYNGYWAD PYTIFFVEIV AMQFAELRRL QDFRYPGSMG QQYFLGLEAI FKGSGDAAYP GGPFFNLFNL G KTEAAMKE ...String:
LYVGASQSSL AYLDGSLPGD FGFDPLGLLD PVNSGGFIEP KWLQYSEVIH ARWAMLGAAG CIAPEVLGAA GLIPDATNIK WFESGVIPP AGSYNGYWAD PYTIFFVEIV AMQFAELRRL QDFRYPGSMG QQYFLGLEAI FKGSGDAAYP GGPFFNLFNL G KTEAAMKE LKLKEIKNGR LAMLAMLGYG AQAVMTGKGP FQNLVEHLAD PVNNNILTNF G

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Macromolecule #14: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 23.304396 KDa
SequenceString: VRPVWFPGNP PPAHLDGSLA GDYGFDPLFL GQEPQTLKWY VQAELVHGRF AMLGAAGIIL TSIGAKVGLG FPEWYDAGKV VVEKNNIDF PTLMVIQFYL MGWAETKRWY DFKNPGSQAD GSFLGFTEEF KGLENGYPGG RFFDPMGLSR GDAAKYQEYK Q KEVKNGRL ...String:
VRPVWFPGNP PPAHLDGSLA GDYGFDPLFL GQEPQTLKWY VQAELVHGRF AMLGAAGIIL TSIGAKVGLG FPEWYDAGKV VVEKNNIDF PTLMVIQFYL MGWAETKRWY DFKNPGSQAD GSFLGFTEEF KGLENGYPGG RFFDPMGLSR GDAAKYQEYK Q KEVKNGRL AMIACLGFAA QYAATGKGPL DNLADHLADP NHVNFATNGV SIPIA

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Macromolecule #15: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 23.293613 KDa
SequenceString: RQSWLPGSQI PAHLDTPAAQ ALAGNFGFDP LGLGKDPVAL RWYQQAELIH CRTAMAGVAG ILIPGLLTKA GALNVPEWYD AGKVAIENS FAPWGSLLAV QLFLCGFVEA KRWQDIRKPG SQGEPGSFLG FEASLKGTSE LGYPGGPFDP LGLSKEADKW A DWKLKEVK ...String:
RQSWLPGSQI PAHLDTPAAQ ALAGNFGFDP LGLGKDPVAL RWYQQAELIH CRTAMAGVAG ILIPGLLTKA GALNVPEWYD AGKVAIENS FAPWGSLLAV QLFLCGFVEA KRWQDIRKPG SQGEPGSFLG FEASLKGTSE LGYPGGPFDP LGLSKEADKW A DWKLKEVK NGRLAMLAFL GFVAQKYATG AGPVDNLAAH LKDPWHVNYA TNGVSLPFL

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Macromolecule #16: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 23.018188 KDa
SequenceString: DAALPSWMPG ADLPGYLNGT LPGDFGFDPL YLGQDPVKLK WYAQAELMNA RFAMLAVAGI LVPELLSNIG FSWPGAGVAW YDAGKFEYF APASSLFGVQ MLLFAWVEIR RYQDFVKPGS ANQDPIFTNN KLPDGNEPGY PGGIFDPFGW SKGDIKSLKL K EIKNGRLA ...String:
DAALPSWMPG ADLPGYLNGT LPGDFGFDPL YLGQDPVKLK WYAQAELMNA RFAMLAVAGI LVPELLSNIG FSWPGAGVAW YDAGKFEYF APASSLFGVQ MLLFAWVEIR RYQDFVKPGS ANQDPIFTNN KLPDGNEPGY PGGIFDPFGW SKGDIKSLKL K EIKNGRLA MLAFAGFIGQ AYTTGTTPLK NLSTHLADPW STTVWQNDLA RL

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Macromolecule #17: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 25.122805 KDa
SequenceString: DRKLWAPGVV APEYLKGDLA GDYGWDPLGL GADPTALKWY RQSELQHARW AMLGVAGVLV QEIVKPDVYF YEAGLPQNLP EPFTNINMG GLLAWEFILM HWVEVRRWQD YKNFGSVNED PIFKGNKVPN PEMGYPGGIF DPFGFSKGNL KELQTKEIKN G RLAMIAYM ...String:
DRKLWAPGVV APEYLKGDLA GDYGWDPLGL GADPTALKWY RQSELQHARW AMLGVAGVLV QEIVKPDVYF YEAGLPQNLP EPFTNINMG GLLAWEFILM HWVEVRRWQD YKNFGSVNED PIFKGNKVPN PEMGYPGGIF DPFGFSKGNL KELQTKEIKN G RLAMIAYM AFILQAQATG KGPLAALSAH LSNPFGNNIL KNIGTCTVPH SVDVQGLTIP LTCLWPGSQ

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Macromolecule #18: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 24.937922 KDa
SequenceString: RPLWLPGSTP PAHLKGDLPG DFGFDPLGLG ANAESLKWFK ESELVHSRWA MAAVAGILVQ EIVRPDVFWY NAGKEVESPL GPLGLLAVE FFLMHWVEVR RWQDLRKPGS VDQDPIFSQY KLPPHEVGYP GGVFAPFIPG DLAELKVKEI KNGRLAMLAF V GFVMAAQV ...String:
RPLWLPGSTP PAHLKGDLPG DFGFDPLGLG ANAESLKWFK ESELVHSRWA MAAVAGILVQ EIVRPDVFWY NAGKEVESPL GPLGLLAVE FFLMHWVEVR RWQDLRKPGS VDQDPIFSQY KLPPHEVGYP GGVFAPFIPG DLAELKVKEI KNGRLAMLAF V GFVMAAQV TGKGPIAALQ EHLADPWGTT IFSKAAVVPG QAVAPPCKIP ASVSYKGIEI PTPCFLQGLW P

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Macromolecule #19: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 21.6581 KDa
SequenceString: RPMWYPGATA PAHLDGSMLG DYGFDPLRLG VNKDNLKWFR EAELTNGRWA MAAVVGILFT DAVGLPKFWT AGAEKYALDN QTLALIEVA VFAVLEGKRY EIYKKTGETG FLSFAPFDPM GMKSEEMKLK ELKNGRLAML AFLGFCSQAA VYGKGPIETL Q LHLADPGH ...String:
RPMWYPGATA PAHLDGSMLG DYGFDPLRLG VNKDNLKWFR EAELTNGRWA MAAVVGILFT DAVGLPKFWT AGAEKYALDN QTLALIEVA VFAVLEGKRY EIYKKTGETG FLSFAPFDPM GMKSEEMKLK ELKNGRLAML AFLGFCSQAA VYGKGPIETL Q LHLADPGH NNIYTSSVGP ETAVTVAVLC VLPMIIEATK

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Macromolecule #20: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant)
Molecular weightTheoretical: 19.929818 KDa
SequenceString:
RPTWLPGLNP PAHLKGALAG DNGFDPLGLG QDEGRLKWYA EAEKTNGRWA MMAVAGILGQ ELLGVTPAWW EAGAKEYDIP AQALTPIEF IVMGFLEIKR YQGFKQTGTS GFINSFPFDP AGMNSPSMAT KEVKNGRLAM VAFIGFCVQA LATRTQPIEG L TAHLADPF GKNITYYLTH LPETL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 12418 / Average electron dose: 46.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal magnification: 165000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1208518
CTF correctionSoftware - Name: CTFFIND (ver. 4.1)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6ijo

Final reconstructionResolution.type: BY AUTHOR / Resolution: 17.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.7) / Number images used: 5707
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.0.7)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.0.7)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6jo5

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7o01:
Dimeric Photosystem I of a temperature sensitive mutant Chlamydomonas reinhardtii

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