- EMDB-12454: The cryoEM density map hexamer from PFO perforated VLPs in the pr... -
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基本情報
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データベース: EMDB / ID: EMD-12454
タイトル
The cryoEM density map hexamer from PFO perforated VLPs in the presence of IP6/CypA
マップデータ
merged map low pass to 6 angstrom and b-factor -100
試料
ウイルス: Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
タンパク質・ペプチド: HIV-1 capsid
機能・相同性
機能・相同性情報
integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / 2-LTR circle formation / Uncoating of the HIV Virion / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus ...integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / 2-LTR circle formation / Uncoating of the HIV Virion / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / Binding and entry of HIV virion / viral life cycle / viral process / Assembly Of The HIV Virion / HIV-1 retropepsin / retroviral ribonuclease H / exoribonuclease H / Budding and maturation of HIV virion / exoribonuclease H activity / protein processing / host multivesicular body / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / host cell / viral capsid / RNA-DNA hybrid ribonuclease activity / 転移酵素; リンを含む基を移すもの; 核酸を移すもの / peptidase activity / symbiont-mediated suppression of host gene expression / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / 加水分解酵素; エステル加水分解酵素 / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / DNA binding / zinc ion binding / identical protein binding / membrane 類似検索 - 分子機能
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/S003339/1
英国
Wellcome Trust
206422/Z/17/Z
英国
Wellcome Trust
203141/Z/16/Z
英国
引用
ジャーナル: Sci Adv / 年: 2021 タイトル: Structure of native HIV-1 cores and their interactions with IP6 and CypA. 著者: Tao Ni / Yanan Zhu / Zhengyi Yang / Chaoyi Xu / Yuriy Chaban / Tanya Nesterova / Jiying Ning / Till Böcking / Michael W Parker / Christina Monnie / Jinwoo Ahn / Juan R Perilla / Peijun Zhang / 要旨: The viral capsid plays essential roles in HIV replication and is a major platform engaging host factors. To overcome challenges in study native capsid structure, we used the perfringolysin O to ...The viral capsid plays essential roles in HIV replication and is a major platform engaging host factors. To overcome challenges in study native capsid structure, we used the perfringolysin O to perforate the membrane of HIV-1 particles, thus allowing host proteins and small molecules to access the native capsid while improving cryo–electron microscopy image quality. Using cryo–electron tomography and subtomogram averaging, we determined the structures of native capsomers in the presence and absence of inositol hexakisphosphate (IP6) and cyclophilin A and constructed an all-atom model of a complete HIV-1 capsid. Our structures reveal two IP6 binding sites and modes of cyclophilin A interactions. Free energy calculations substantiate the two binding sites at R18 and K25 and further show a prohibitive energy barrier for IP6 to pass through the pentamer. Our results demonstrate that perfringolysin O perforation is a valuable tool for structural analyses of enveloped virus capsids and interactions with host cell factors.