- EMDB-11948: Axonemal 96nm-repeat from active flagella of wild type mouse, sho... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-11948
タイトル
Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations. Reconstruction obtained with classification and averaging of sub-tomograms from cryo-electron tomograms of wild type active flagella.
マップデータ
Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations.
ジャーナル: Science / 年: 2021 タイトル: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. 著者: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis ...著者: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis Alvarez / Gaia Pigino / Carsten Janke / 要旨: Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. ...Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
ダウンロード / ファイル: emd_11948.map.gz / 形式: CCP4 / 大きさ: 3.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations.
ボクセルのサイズ
X=Y=Z: 9.422 Å
密度
表面レベル
登録者による: 6.12 / ムービー #1: 6.12
最小 - 最大
-40.844482 - 43.06595
平均 (標準偏差)
1.0526515 (±7.9630036)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
43
46
サイズ
160
72
88
Spacing
72
160
88
セル
A: 678.384 Å / B: 1507.52 Å / C: 829.136 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
9.422
9.422
9.422
M x/y/z
72
160
88
origin x/y/z
0.000
0.000
0.000
length x/y/z
678.384
1507.520
829.136
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
43
0
46
NC/NR/NS
72
160
88
D min/max/mean
-40.844
43.066
1.053
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添付データ
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試料の構成要素
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全体 : Axonemal 96nm-repeat
全体
名称: Axonemal 96nm-repeat
要素
複合体: Axonemal 96nm-repeat
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超分子 #1: Axonemal 96nm-repeat
超分子
名称: Axonemal 96nm-repeat / タイプ: complex / ID: 1 / 親要素: 0 詳細: Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations. Reconstruction obtained with classification and averaging of ...詳細: Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations. Reconstruction obtained with classification and averaging of sub-tomograms from cryo-electron tomograms of wild type active flagella.