"Tubulin glycylation controls axonemal dynein activity, flagellar beat and male fertility": Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
Map data
Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both %u03B2- and %u03B3-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
Sample
Complex: axonemal outer dynein arm (ODA)
Biological species
Mus musculus (house mouse)
Method
subtomogram averaging / cryo EM / Resolution: 24.7 Å
Journal: Science / Year: 2021 Title: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. Authors: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis ...Authors: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis Alvarez / Gaia Pigino / Carsten Janke / Abstract: Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. ...Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
History
Deposition
Nov 16, 2020
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Header (metadata) release
Jan 20, 2021
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Map release
Jan 20, 2021
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Update
Feb 10, 2021
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Current status
Feb 10, 2021
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_11942.map.gz / Format: CCP4 / Size: 695.3 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both %u03B2- and %u03B3-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
A: 716.072 Å / B: 565.32 Å / C: 367.458 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
9.422
9.422
9.422
M x/y/z
76
60
39
origin x/y/z
0.000
0.000
0.000
length x/y/z
716.072
565.320
367.458
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
32
0
10
NC/NR/NS
76
60
39
D min/max/mean
-22.475
32.507
1.334
-
Supplemental data
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Sample components
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Entire : axonemal outer dynein arm (ODA)
Entire
Name: axonemal outer dynein arm (ODA)
Components
Complex: axonemal outer dynein arm (ODA)
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Supramolecule #1: axonemal outer dynein arm (ODA)
Supramolecule
Name: axonemal outer dynein arm (ODA) / type: complex / ID: 1 / Parent: 0 Details: Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma- heavy chains are in pre-power stroke conformation. Reconstructed with cryo-ET, ...Details: Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma- heavy chains are in pre-power stroke conformation. Reconstructed with cryo-ET, classification and sub-tomogram averaging.
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