- EMDB-11947: Axonemal 96nm-repeat from active sperm flagella of glycylation de... -
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Basic information
Entry
Database: EMDB / ID: EMD-11947
Title
Axonemal 96nm-repeat from active sperm flagella of glycylation deficient mouse (Ttll3-/-Ttll8-/- mutation). Reconstruction obtained with cryo-ET and sub-tomogram averaging.
Map data
Axonemal 96nm-repeat from active sperm flagella of glycylation deficient mouse (Ttll3%u2011/%u2011Ttll8%u2011/%u2011 mutation). Reconstruction obtained with cryo-ET and sub-tomogram averaging.
Sample
Complex: Axonemal 96nm-repeat
Biological species
Mus musculus (house mouse)
Method
subtomogram averaging / cryo EM / Resolution: 33.0 Å
Journal: Science / Year: 2021 Title: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. Authors: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis ...Authors: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis Alvarez / Gaia Pigino / Carsten Janke / Abstract: Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. ...Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
History
Deposition
Nov 17, 2020
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Header (metadata) release
Jan 20, 2021
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Map release
Jan 20, 2021
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Update
Jan 20, 2021
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Current status
Jan 20, 2021
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_11947.map.gz / Format: CCP4 / Size: 3.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Axonemal 96nm-repeat from active sperm flagella of glycylation deficient mouse (Ttll3%u2011/%u2011Ttll8%u2011/%u2011 mutation). Reconstruction obtained with cryo-ET and sub-tomogram averaging.
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