German Federal Ministry for Education and Research
81Z0600301
ドイツ
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: Molecular-scale visualization of sarcomere contraction within native cardiomyocytes. 著者: Laura Burbaum / Jonathan Schneider / Sarah Scholze / Ralph T Böttcher / Wolfgang Baumeister / Petra Schwille / Jürgen M Plitzko / Marion Jasnin / 要旨: Sarcomeres, the basic contractile units of striated muscle, produce the forces driving muscular contraction through cross-bridge interactions between actin-containing thin filaments and myosin II- ...Sarcomeres, the basic contractile units of striated muscle, produce the forces driving muscular contraction through cross-bridge interactions between actin-containing thin filaments and myosin II-based thick filaments. Until now, direct visualization of the molecular architecture underlying sarcomere contractility has remained elusive. Here, we use in situ cryo-electron tomography to unveil sarcomere contraction in frozen-hydrated neonatal rat cardiomyocytes. We show that the hexagonal lattice of the thick filaments is already established at the neonatal stage, with an excess of thin filaments outside the trigonal positions. Structural assessment of actin polarity by subtomogram averaging reveals that thin filaments in the fully activated state form overlapping arrays of opposite polarity in the center of the sarcomere. Our approach provides direct evidence for thin filament sliding during muscle contraction and may serve as a basis for structural understanding of thin filament activation and actomyosin interactions inside unperturbed cellular environments.
全体 : Sarcomeric actin-tropomyosin filament in the myosin state within ...
全体
名称: Sarcomeric actin-tropomyosin filament in the myosin state within the native cellular environment of neonatal Wistar rat cardiomyocytes
要素
複合体: Sarcomeric actin-tropomyosin filament in the myosin state within the native cellular environment of neonatal Wistar rat cardiomyocytes
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超分子 #1: Sarcomeric actin-tropomyosin filament in the myosin state within ...
超分子
名称: Sarcomeric actin-tropomyosin filament in the myosin state within the native cellular environment of neonatal Wistar rat cardiomyocytes タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1