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TitleMolecular-scale visualization of sarcomere contraction within native cardiomyocytes.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 4086, Year 2021
Publish dateJul 2, 2021
AuthorsLaura Burbaum / Jonathan Schneider / Sarah Scholze / Ralph T Böttcher / Wolfgang Baumeister / Petra Schwille / Jürgen M Plitzko / Marion Jasnin /
PubMed AbstractSarcomeres, the basic contractile units of striated muscle, produce the forces driving muscular contraction through cross-bridge interactions between actin-containing thin filaments and myosin II- ...Sarcomeres, the basic contractile units of striated muscle, produce the forces driving muscular contraction through cross-bridge interactions between actin-containing thin filaments and myosin II-based thick filaments. Until now, direct visualization of the molecular architecture underlying sarcomere contractility has remained elusive. Here, we use in situ cryo-electron tomography to unveil sarcomere contraction in frozen-hydrated neonatal rat cardiomyocytes. We show that the hexagonal lattice of the thick filaments is already established at the neonatal stage, with an excess of thin filaments outside the trigonal positions. Structural assessment of actin polarity by subtomogram averaging reveals that thin filaments in the fully activated state form overlapping arrays of opposite polarity in the center of the sarcomere. Our approach provides direct evidence for thin filament sliding during muscle contraction and may serve as a basis for structural understanding of thin filament activation and actomyosin interactions inside unperturbed cellular environments.
External linksNat Commun / PubMed:34215727 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution15.7 - 17.7 Å
Structure data

EMDB-11825:
In situ subtomogram average of the sarcomeric actin-tropomyosin filament from neonatal Wistar rat cardiomyocytes in an intermediate state
Method: EM (subtomogram averaging) / Resolution: 17.7 Å

EMDB-11826:
In situ subtomogram average of the sarcomeric actin-tropomyosin filament from neonatal Wistar rat cardiomyocytes in the myosin state
Method: EM (subtomogram averaging) / Resolution: 15.7 Å

EMDB-12572:
Tomogram of myofibrils within a native neonatal Wistar rat cardiomyocyte
Method: EM (tomography)

Source
  • Rattus norvegicus (Norway rat)

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