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- EMDB-0830: Structure of the human sterol O-acyltransferase 1 tetramer in rho... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0830 | ||||||||||||||||||
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Title | Structure of the human sterol O-acyltransferase 1 tetramer in rhombic shape | ||||||||||||||||||
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Biological species | ![]() | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.6 Å | ||||||||||||||||||
![]() | Chen L / Guan C | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the inhibition mechanism of human sterol O-acyltransferase 1 by a competitive inhibitor. Authors: Chengcheng Guan / Yange Niu / Si-Cong Chen / Yunlu Kang / Jing-Xiang Wu / Koji Nishi / Catherine C Y Chang / Ta-Yuan Chang / Tuoping Luo / Lei Chen / ![]() ![]() Abstract: Sterol O-acyltransferase 1 (SOAT1) is an endoplasmic reticulum (ER) resident, multi-transmembrane enzyme that belongs to the membrane-bound O-acyltransferase (MBOAT) family. It catalyzes the ...Sterol O-acyltransferase 1 (SOAT1) is an endoplasmic reticulum (ER) resident, multi-transmembrane enzyme that belongs to the membrane-bound O-acyltransferase (MBOAT) family. It catalyzes the esterification of cholesterol to generate cholesteryl esters for cholesterol storage. SOAT1 is a target to treat several human diseases. However, its structure and mechanism remain elusive since its discovery. Here, we report the structure of human SOAT1 (hSOAT1) determined by cryo-EM. hSOAT1 is a tetramer consisted of a dimer of dimer. The structure of hSOAT1 dimer at 3.5 Å resolution reveals that a small molecule inhibitor CI-976 binds inside the catalytic chamber and blocks the accessibility of the active site residues H460, N421 and W420. Our results pave the way for future mechanistic study and rational drug design targeting hSOAT1 and other mammalian MBOAT family members. | ||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 20.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.4 KB 10.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.4 KB | Display | ![]() |
Images | ![]() | 91.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 78.4 KB | Display | ![]() |
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Full document | ![]() | 77.5 KB | Display | |
Data in XML | ![]() | 494 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.045 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : human sterol O-acyltransferase 1 tetramer
Entire | Name: human sterol O-acyltransferase 1 tetramer |
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Components |
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-Supramolecule #1: human sterol O-acyltransferase 1 tetramer
Supramolecule | Name: human sterol O-acyltransferase 1 tetramer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
-Macromolecule #1: Structure of the human sterol O-acyltransferase 1
Macromolecule | Name: Structure of the human sterol O-acyltransferase 1 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Sequence | String: MVGEEKMSLR NRLSKSRENP EEDEDQRNPA KESLETPSNG RIDIKQLIAK KIKLTAEAEE LKPFFMKEVG SHFDDFVTNL IEKSASLDN GGCALTTFSV LEGEKNNHRA KDLRAPPEQG KIFIARRSLL DELLEVDHIR TIYHMFIALL ILFILSTLVV D YIDEGRLV ...String: MVGEEKMSLR NRLSKSRENP EEDEDQRNPA KESLETPSNG RIDIKQLIAK KIKLTAEAEE LKPFFMKEVG SHFDDFVTNL IEKSASLDN GGCALTTFSV LEGEKNNHRA KDLRAPPEQG KIFIARRSLL DELLEVDHIR TIYHMFIALL ILFILSTLVV D YIDEGRLV LEFSLLSYAF GKFPTVVWTW WIMFLSTFSV PYFLFQHWAT GYSKSSHPLI RSLFHGFLFM IFQIGVLGFG PT YVVLAYT LPPASRFIII FEQIRFVMKA HSFVRENVPR VLNSAKEKSS TVPIPTVNQY LYFLFAPTLI YRDSYPRNPT VRW GYVAMK FAQVFGCFFY VYYIFERLCA PLFRNIKQEP FSARVLVLCV FNSILPGVLI LFLTFFAFLH CWLNAFAEML RFGD RMFYK DWWNSTSYSN YYRTWNVVVH DWLYYYAYKD FLWFFSKRFK SAAMLAVFAV SAVVHEYALA VCLSFFYPVL FVLFM FFGM AFNFIVNDSR KKPIWNVLMW TSLFLGNGVL LCFYSQEWYA RQHCPLKNPT FLDYVRPRSW TCRYVF |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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